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Protein

T-lymphocyte activation antigen CD86

Gene

CD86

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
(Microbial infection) Acts as a receptor for adenovirus subgroup B.1 Publication

GO - Molecular functioni

  • coreceptor activity Source: UniProtKB
  • phosphatidylinositol-4,5-bisphosphate 3-kinase activity Source: Reactome
  • receptor activity Source: ProtInc
  • virus receptor activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Host cell receptor for virus entry, Receptor

Keywords - Biological processi

Adaptive immunity, Host-virus interaction, Immunity

Enzyme and pathway databases

BioCyciZFISH:ENSG00000114013-MONOMER.
ReactomeiR-HSA-1257604. PIP3 activates AKT signaling.
R-HSA-2219530. Constitutive Signaling by Aberrant PI3K in Cancer.
R-HSA-389356. CD28 co-stimulation.
R-HSA-389357. CD28 dependent PI3K/Akt signaling.
R-HSA-389359. CD28 dependent Vav1 pathway.
R-HSA-389513. CTLA4 inhibitory signaling.
R-HSA-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
T-lymphocyte activation antigen CD86
Alternative name(s):
Activation B7-2 antigen
B70
BU63
CTLA-4 counter-receptor B7.2
FUN-1
CD_antigen: CD86
Gene namesi
Name:CD86
Synonyms:CD28LG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:1705. CD86.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 247ExtracellularSequence analysisAdd BLAST224
Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
Topological domaini269 – 329CytoplasmicSequence analysisAdd BLAST61

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • external side of plasma membrane Source: Ensembl
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB
  • intracellular membrane-bounded organelle Source: Ensembl
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi942.
OpenTargetsiENSG00000114013.
PharmGKBiPA26243.

Chemistry databases

ChEMBLiCHEMBL2364156.
DrugBankiDB01281. Abatacept.
DB00098. Anti-thymocyte Globulin (Rabbit).
DB06681. Belatacept.
GuidetoPHARMACOLOGYi2745.

Polymorphism and mutation databases

BioMutaiCD86.
DMDMi317373339.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001455024 – 329T-lymphocyte activation antigen CD86Add BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi40 ↔ 110PROSITE-ProRule annotation1 Publication
Glycosylationi47N-linked (GlcNAc...)Sequence analysis1
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Glycosylationi146N-linked (GlcNAc...)Sequence analysis1
Glycosylationi154N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi157 ↔ 218PROSITE-ProRule annotation
Glycosylationi177N-linked (GlcNAc...)Sequence analysis1
Glycosylationi192N-linked (GlcNAc...)Sequence analysis1
Glycosylationi213N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiP42081.
PeptideAtlasiP42081.
PRIDEiP42081.

PTM databases

iPTMnetiP42081.
PhosphoSitePlusiP42081.

Expressioni

Tissue specificityi

Expressed by activated B-lymphocytes and monocytes.

Gene expression databases

BgeeiENSG00000114013.
CleanExiHS_CD86.
ExpressionAtlasiP42081. baseline and differential.
GenevisibleiP42081. HS.

Organism-specific databases

HPAiCAB004319.

Interactioni

Subunit structurei

Homodimer. Interacts with MARCH8.Curated3 Publications
(Microbial infection) Interacts with adenovirus subgroup b fiber protein.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CTLA4P164103EBI-1030956,EBI-1030991

Protein-protein interaction databases

BioGridi107380. 6 interactors.
DIPiDIP-35606N.
IntActiP42081. 4 interactors.
MINTiMINT-6631610.
STRINGi9606.ENSP00000332049.

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 31Combined sources6
Beta strandi36 – 39Combined sources4
Helixi50 – 52Combined sources3
Beta strandi53 – 59Combined sources7
Beta strandi64 – 69Combined sources6
Beta strandi76 – 78Combined sources3
Turni80 – 84Combined sources5
Beta strandi85 – 89Combined sources5
Turni90 – 93Combined sources4
Beta strandi94 – 97Combined sources4
Helixi102 – 104Combined sources3
Beta strandi106 – 115Combined sources10
Beta strandi117 – 133Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I85X-ray3.20A/B26-134[»]
1NCNX-ray2.70A/B26-134[»]
ProteinModelPortaliP42081.
SMRiP42081.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42081.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 131Ig-like V-typeAdd BLAST99
Domaini150 – 225Ig-like C2-typeAdd BLAST76

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYP1. Eukaryota.
ENOG410ZD66. LUCA.
GeneTreeiENSGT00720000108819.
HOGENOMiHOG000276893.
HOVERGENiHBG004093.
InParanoidiP42081.
KOiK05413.
OMAiDKGLYQC.
OrthoDBiEOG091G0QVY.
PhylomeDBiP42081.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF13895. Ig_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42081-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPQCTMGLS NILFVMAFLL SGAAPLKIQA YFNETADLPC QFANSQNQSL
60 70 80 90 100
SELVVFWQDQ ENLVLNEVYL GKEKFDSVHS KYMGRTSFDS DSWTLRLHNL
110 120 130 140 150
QIKDKGLYQC IIHHKKPTGM IRIHQMNSEL SVLANFSQPE IVPISNITEN
160 170 180 190 200
VYINLTCSSI HGYPEPKKMS VLLRTKNSTI EYDGVMQKSQ DNVTELYDVS
210 220 230 240 250
ISLSVSFPDV TSNMTIFCIL ETDKTRLLSS PFSIELEDPQ PPPDHIPWIT
260 270 280 290 300
AVLPTVIICV MVFCLILWKW KKKKRPRNSY KCGTNTMERE ESEQTKKREK
310 320
IHIPERSDEA QRVFKSSKTS SCDKSDTCF
Length:329
Mass (Da):37,682
Last modified:January 11, 2011 - v2
Checksum:iC249DAEEB889D911
GO
Isoform 2 (identifier: P42081-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Show »
Length:323
Mass (Da):37,007
Checksum:iA492C705D89B65B7
GO
Isoform 3 (identifier: P42081-2) [UniParc]FASTAAdd to basket
Also known as: CD86 deltaEC

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.
     22-234: Missing.

Show »
Length:110
Mass (Da):12,818
Checksum:i0AE70C853C85F666
GO
Isoform 4 (identifier: P42081-4) [UniParc]FASTAAdd to basket
Also known as: CD86 deltaTM

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.
     235-282: Missing.

Show »
Length:275
Mass (Da):31,293
Checksum:i137A523F73708BD5
GO
Isoform 5 (identifier: P42081-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     22-133: Missing.

Note: No experimental confirmation available.
Show »
Length:217
Mass (Da):24,773
Checksum:i7AF72282EEF8B7CE
GO
Isoform 6 (identifier: P42081-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Note: No experimental confirmation available.
Show »
Length:247
Mass (Da):28,420
Checksum:i60CBC08652F42C26
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27K → E in AAA86473 (PubMed:7541777).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021916170S → N.Corresponds to variant rs9282642dbSNPEnsembl.1
Natural variantiVAR_055003185V → I.8 PublicationsCorresponds to variant rs2681417dbSNPEnsembl.1
Natural variantiVAR_014650310A → T.2 PublicationsCorresponds to variant rs1129055dbSNPEnsembl.1
Natural variantiVAR_021917323D → N.Corresponds to variant rs9282648dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0472201 – 82Missing in isoform 6. CuratedAdd BLAST82
Alternative sequenceiVSP_0231241 – 6Missing in isoform 2, isoform 3 and isoform 4. 3 Publications6
Alternative sequenceiVSP_00912522 – 234Missing in isoform 3. 1 PublicationAdd BLAST213
Alternative sequenceiVSP_04722122 – 133Missing in isoform 5. 1 PublicationAdd BLAST112
Alternative sequenceiVSP_040324235 – 282Missing in isoform 4. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04343 mRNA. Translation: AAB03814.1.
L25259 mRNA. Translation: AAA58389.1.
CR541844 mRNA. Translation: CAG46642.1.
EF064748 Genomic DNA. Translation: ABK41931.1.
AK294663 mRNA. Translation: BAH11839.1.
AK301237 mRNA. Translation: BAH13438.1.
AK316203 mRNA. Translation: BAH14574.1.
AC068630 Genomic DNA. No translation available.
BC040261 mRNA. Translation: AAH40261.1.
U17722
, U17717, U17718, U17719, U17721 Genomic DNA. Translation: AAA86473.1.
CCDSiCCDS3009.1. [P42081-1]
CCDS43138.1. [P42081-3]
CCDS56272.1. [P42081-5]
CCDS56273.1. [P42081-6]
CCDS74991.1. [P42081-4]
PIRiA48754.
JC7605.
RefSeqiNP_001193853.1. NM_001206924.1.
NP_001193854.1. NM_001206925.1.
NP_008820.3. NM_006889.4.
NP_787058.4. NM_175862.4.
NP_795711.1. NM_176892.1.
UniGeneiHs.171182.

Genome annotation databases

EnsembliENST00000264468; ENSP00000264468; ENSG00000114013. [P42081-4]
ENST00000330540; ENSP00000332049; ENSG00000114013. [P42081-1]
ENST00000393627; ENSP00000377248; ENSG00000114013. [P42081-3]
ENST00000469710; ENSP00000418988; ENSG00000114013. [P42081-6]
ENST00000493101; ENSP00000420230; ENSG00000114013. [P42081-5]
GeneIDi942.
KEGGihsa:942.
UCSCiuc003eet.4. human. [P42081-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

CD86 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04343 mRNA. Translation: AAB03814.1.
L25259 mRNA. Translation: AAA58389.1.
CR541844 mRNA. Translation: CAG46642.1.
EF064748 Genomic DNA. Translation: ABK41931.1.
AK294663 mRNA. Translation: BAH11839.1.
AK301237 mRNA. Translation: BAH13438.1.
AK316203 mRNA. Translation: BAH14574.1.
AC068630 Genomic DNA. No translation available.
BC040261 mRNA. Translation: AAH40261.1.
U17722
, U17717, U17718, U17719, U17721 Genomic DNA. Translation: AAA86473.1.
CCDSiCCDS3009.1. [P42081-1]
CCDS43138.1. [P42081-3]
CCDS56272.1. [P42081-5]
CCDS56273.1. [P42081-6]
CCDS74991.1. [P42081-4]
PIRiA48754.
JC7605.
RefSeqiNP_001193853.1. NM_001206924.1.
NP_001193854.1. NM_001206925.1.
NP_008820.3. NM_006889.4.
NP_787058.4. NM_175862.4.
NP_795711.1. NM_176892.1.
UniGeneiHs.171182.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I85X-ray3.20A/B26-134[»]
1NCNX-ray2.70A/B26-134[»]
ProteinModelPortaliP42081.
SMRiP42081.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107380. 6 interactors.
DIPiDIP-35606N.
IntActiP42081. 4 interactors.
MINTiMINT-6631610.
STRINGi9606.ENSP00000332049.

Chemistry databases

ChEMBLiCHEMBL2364156.
DrugBankiDB01281. Abatacept.
DB00098. Anti-thymocyte Globulin (Rabbit).
DB06681. Belatacept.
GuidetoPHARMACOLOGYi2745.

PTM databases

iPTMnetiP42081.
PhosphoSitePlusiP42081.

Polymorphism and mutation databases

BioMutaiCD86.
DMDMi317373339.

Proteomic databases

PaxDbiP42081.
PeptideAtlasiP42081.
PRIDEiP42081.

Protocols and materials databases

DNASUi942.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264468; ENSP00000264468; ENSG00000114013. [P42081-4]
ENST00000330540; ENSP00000332049; ENSG00000114013. [P42081-1]
ENST00000393627; ENSP00000377248; ENSG00000114013. [P42081-3]
ENST00000469710; ENSP00000418988; ENSG00000114013. [P42081-6]
ENST00000493101; ENSP00000420230; ENSG00000114013. [P42081-5]
GeneIDi942.
KEGGihsa:942.
UCSCiuc003eet.4. human. [P42081-1]

Organism-specific databases

CTDi942.
DisGeNETi942.
GeneCardsiCD86.
HGNCiHGNC:1705. CD86.
HPAiCAB004319.
MIMi601020. gene.
neXtProtiNX_P42081.
OpenTargetsiENSG00000114013.
PharmGKBiPA26243.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IYP1. Eukaryota.
ENOG410ZD66. LUCA.
GeneTreeiENSGT00720000108819.
HOGENOMiHOG000276893.
HOVERGENiHBG004093.
InParanoidiP42081.
KOiK05413.
OMAiDKGLYQC.
OrthoDBiEOG091G0QVY.
PhylomeDBiP42081.
TreeFamiTF331083.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000114013-MONOMER.
ReactomeiR-HSA-1257604. PIP3 activates AKT signaling.
R-HSA-2219530. Constitutive Signaling by Aberrant PI3K in Cancer.
R-HSA-389356. CD28 co-stimulation.
R-HSA-389357. CD28 dependent PI3K/Akt signaling.
R-HSA-389359. CD28 dependent Vav1 pathway.
R-HSA-389513. CTLA4 inhibitory signaling.
R-HSA-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Miscellaneous databases

ChiTaRSiCD86. human.
EvolutionaryTraceiP42081.
GeneWikiiCD86.
GenomeRNAii942.
PROiP42081.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114013.
CleanExiHS_CD86.
ExpressionAtlasiP42081. baseline and differential.
GenevisibleiP42081. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF13895. Ig_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD86_HUMAN
AccessioniPrimary (citable) accession number: P42081
Secondary accession number(s): A0N0P0
, B7Z2F3, B7Z702, E7ETN5, E9PC27, Q13655, Q6FHB1, Q6GTS4, Q7M4L5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 11, 2011
Last modified: November 2, 2016
This is version 171 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.