Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P42073 (REF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA end formation protein 2
Gene names
Name:REF2
Ordered Locus Names:YDR195W
ORF Names:YD9346.06
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Negative regulator of poly(A) synthesis. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. REF2 is required for 3'-end formation of snoRNAs. Ref.4 Ref.5 Ref.6 Ref.9

Subunit structure

Interacts with FIR1. Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2. Ref.5 Ref.7

Subcellular location

Nucleus Ref.7.

Miscellaneous

Present with 7450 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GLC7P325987EBI-14915,EBI-13715

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533RNA end formation protein 2
PRO_0000097239

Regions

Compositional bias321 – 33111Ser/Thr-rich

Sequences

Sequence LengthMass (Da)Tools
P42073 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 36B0982B005697C4

FASTA53359,819
        10         20         30         40         50         60 
MSAPVPQLVN ISHALQASTI QQIRLDMVDF NKDCKLSSIQ LARIDKYIDS LQAALNQFTK 

        70         80         90        100        110        120 
DNLHIERKEK NVTEADIQLY SGLKSMYLDY LNQLIKLKHE KQHHSTPPIA NDVSLDFFVN 

       130        140        150        160        170        180 
QLPKFSPEER KNYIDNLILN KNSHNRLSKM DGLVDAVINL CVLDTSVAEN VRSYMKLLDT 

       190        200        210        220        230        240 
LGFQKGSNST GTKANLKKKL ASSKAKIKDS EKEKEKEKDK SKVKMKTKLK PSPLLNNDDK 

       250        260        270        280        290        300 
NSSPSPTAST SSMKKLKSGL FNKNEAKSTE SLPTSSKKKL SFSKYLNKDD ADMTKLGTKR 

       310        320        330        340        350        360 
SIDVDFKVNP EASTVASNII SSSTSGSSTT TVATPASSEE PLKKKTKISV QDSNVQSILR 

       370        380        390        400        410        420 
NGKPKKARIS SIKFLDDSQL IKVYGDDLPN QGLQVSPTQL KKILKPFKEG EPKEIILFED 

       430        440        450        460        470        480 
MSIKLKPLDL MFLKNTNSDD YMDISETKGG PIHCETRTPL IYRKNFNHFN PDLNKRPPRE 

       490        500        510        520        530 
PIEFDLNGNT NSTPTIAKAF GKNSLLLRKD RGGLPYKHVP IVKRNKYPPR PVH 

« Hide

References

« Hide 'large scale' references
[1]Russnak R.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"REF2 encodes an RNA-binding protein directly involved in yeast mRNA 3'-end formation."
Russnak R., Nehrke K.W., Platt T.
Mol. Cell. Biol. 15:1689-1697(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE OF 1-429, FUNCTION.
[5]"RNA binding analysis of yeast REF2 and its two-hybrid interaction with a new gene product, FIR1."
Russnak R., Pereira S., Platt T.
Gene Expr. 6:241-258(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FIR1.
[6]"Pti1p and Ref2p found in association with the mRNA 3' end formation complex direct snoRNA maturation."
Dheur S., Vo le T.A., Voisinet-Hakil F., Minet M., Schmitter J.-M., Lacroute F., Wyers F., Minvielle-Sebastia L.
EMBO J. 22:2831-2840(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN SNORNA 3-END FORMATION.
[7]"Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CPF COMPLEX, COMPOSITION OF THE APT COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Identification of factors regulating poly(A) tail synthesis and maturation."
Mangus D.A., Smith M.M., McSweeney J.M., Jacobson A.
Mol. Cell. Biol. 24:4196-4206(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20261 Genomic DNA. Translation: AAA85866.1.
Z48784 Genomic DNA. Translation: CAA88708.1.
BK006938 Genomic DNA. Translation: DAA12038.1.
PIRS52702.
RefSeqNP_010481.3. NM_001180503.3.

3D structure databases

ProteinModelPortalP42073.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32247. 60 interactions.
DIPDIP-871N.
IntActP42073. 31 interactions.
MINTMINT-394246.
STRING4932.YDR195W.

Proteomic databases

PaxDbP42073.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR195W; YDR195W; YDR195W.
GeneID851776.
KEGGsce:YDR195W.

Organism-specific databases

CYGDYDR195w.
SGDS000002603. REF2.

Phylogenomic databases

eggNOGNOG46408.
KOK15543.
OMAINIAHAM.
OrthoDBEOG754HZP.

Enzyme and pathway databases

BioCycYEAST:G3O-29782-MONOMER.

Gene expression databases

GenevestigatorP42073.

Family and domain databases

ProtoNetSearch...

Other

NextBio969576.

Entry information

Entry nameREF2_YEAST
AccessionPrimary (citable) accession number: P42073
Secondary accession number(s): D6VSH8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD