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Protein

RNA end formation protein 2

Gene

REF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Negative regulator of poly(A) synthesis. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. REF2 is required for 3'-end formation of snoRNAs.4 Publications

Miscellaneous

Present with 7450 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • chromatin binding Source: SGD
  • RNA binding Source: SGD

GO - Biological processi

  • mRNA 3'-end processing Source: SGD
  • negative regulation of mRNA polyadenylation Source: SGD
  • positive regulation of mitotic actomyosin contractile ring assembly Source: SGD
  • protein dephosphorylation Source: SGD
  • regulation of protein localization to cell division site involved in cytokinesis Source: SGD
  • snoRNA 3'-end processing Source: SGD
  • termination of RNA polymerase II transcription, poly(A)-coupled Source: SGD

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29782-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA end formation protein 2
Gene namesi
Name:REF2
Ordered Locus Names:YDR195W
ORF Names:YD9346.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR195W
SGDiS000002603 REF2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972391 – 533RNA end formation protein 2Add BLAST533

Proteomic databases

MaxQBiP42073
PaxDbiP42073
PRIDEiP42073

PTM databases

CarbonylDBiP42073
iPTMnetiP42073

Interactioni

Subunit structurei

Interacts with FIR1. Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GLC7P325988EBI-14915,EBI-13715

Protein-protein interaction databases

BioGridi32247, 295 interactors
DIPiDIP-871N
IntActiP42073, 31 interactors
MINTiP42073
STRINGi4932.YDR195W

Structurei

3D structure databases

ProteinModelPortaliP42073
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi321 – 331Ser/Thr-richAdd BLAST11

Phylogenomic databases

InParanoidiP42073
KOiK15543
OMAiGGLPYKR
OrthoDBiEOG092C29T4

Sequencei

Sequence statusi: Complete.

P42073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPVPQLVN ISHALQASTI QQIRLDMVDF NKDCKLSSIQ LARIDKYIDS
60 70 80 90 100
LQAALNQFTK DNLHIERKEK NVTEADIQLY SGLKSMYLDY LNQLIKLKHE
110 120 130 140 150
KQHHSTPPIA NDVSLDFFVN QLPKFSPEER KNYIDNLILN KNSHNRLSKM
160 170 180 190 200
DGLVDAVINL CVLDTSVAEN VRSYMKLLDT LGFQKGSNST GTKANLKKKL
210 220 230 240 250
ASSKAKIKDS EKEKEKEKDK SKVKMKTKLK PSPLLNNDDK NSSPSPTAST
260 270 280 290 300
SSMKKLKSGL FNKNEAKSTE SLPTSSKKKL SFSKYLNKDD ADMTKLGTKR
310 320 330 340 350
SIDVDFKVNP EASTVASNII SSSTSGSSTT TVATPASSEE PLKKKTKISV
360 370 380 390 400
QDSNVQSILR NGKPKKARIS SIKFLDDSQL IKVYGDDLPN QGLQVSPTQL
410 420 430 440 450
KKILKPFKEG EPKEIILFED MSIKLKPLDL MFLKNTNSDD YMDISETKGG
460 470 480 490 500
PIHCETRTPL IYRKNFNHFN PDLNKRPPRE PIEFDLNGNT NSTPTIAKAF
510 520 530
GKNSLLLRKD RGGLPYKHVP IVKRNKYPPR PVH
Length:533
Mass (Da):59,819
Last modified:February 1, 1996 - v2
Checksum:i36B0982B005697C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20261 Genomic DNA Translation: AAA85866.1
Z48784 Genomic DNA Translation: CAA88708.1
BK006938 Genomic DNA Translation: DAA12038.1
PIRiS52702
RefSeqiNP_010481.3, NM_001180503.3

Genome annotation databases

EnsemblFungiiYDR195W; YDR195W; YDR195W
GeneIDi851776
KEGGisce:YDR195W

Similar proteinsi

Entry informationi

Entry nameiREF2_YEAST
AccessioniPrimary (citable) accession number: P42073
Secondary accession number(s): D6VSH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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