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Protein

Oligopeptide-binding protein AppA

Gene

appA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapeptides but not tripeptides.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Competence, Peptide transport, Protein transport, Sporulation, Transport

Protein family/group databases

TCDBi3.A.1.5.20. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligopeptide-binding protein AppA
Gene namesi
Name:appA
Ordered Locus Names:BSU11381/BSU11382
ORF Names:BSU11380
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323PROSITE-ProRule annotationAdd
BLAST
Chaini24 – 543520Oligopeptide-binding protein AppAPRO_0000031791Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi24 – 241N-palmitoyl cysteineCurated
Lipidationi24 – 241S-diacylglycerol cysteineCurated

Keywords - PTMi

Lipoprotein, Palmitate

Structurei

Secondary structure

1
543
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi46 – 538Combined sources
Turni60 – 623Combined sources
Helixi66 – 7510Combined sources
Beta strandi79 – 824Combined sources
Beta strandi88 – 9912Combined sources
Helixi100 – 1023Combined sources
Beta strandi104 – 1096Combined sources
Helixi123 – 1319Combined sources
Helixi132 – 1343Combined sources
Helixi143 – 1453Combined sources
Turni146 – 1483Combined sources
Beta strandi149 – 1568Combined sources
Beta strandi159 – 1668Combined sources
Helixi170 – 1734Combined sources
Helixi175 – 1784Combined sources
Helixi184 – 1874Combined sources
Helixi192 – 1943Combined sources
Turni195 – 1973Combined sources
Helixi199 – 2013Combined sources
Beta strandi208 – 21710Combined sources
Turni218 – 2203Combined sources
Beta strandi221 – 2266Combined sources
Beta strandi236 – 2449Combined sources
Helixi248 – 2569Combined sources
Beta strandi262 – 2643Combined sources
Helixi267 – 2693Combined sources
Helixi270 – 2734Combined sources
Beta strandi279 – 2857Combined sources
Beta strandi287 – 2948Combined sources
Helixi299 – 3013Combined sources
Helixi304 – 3129Combined sources
Helixi316 – 3238Combined sources
Beta strandi328 – 3303Combined sources
Beta strandi333 – 3353Combined sources
Helixi355 – 36410Combined sources
Beta strandi370 – 3756Combined sources
Beta strandi384 – 3907Combined sources
Helixi394 – 40815Combined sources
Turni409 – 4113Combined sources
Beta strandi413 – 4197Combined sources
Helixi421 – 4288Combined sources
Turni430 – 4323Combined sources
Beta strandi436 – 4438Combined sources
Helixi451 – 4544Combined sources
Helixi456 – 4583Combined sources
Turni459 – 4613Combined sources
Helixi471 – 48010Combined sources
Helixi486 – 50318Combined sources
Beta strandi505 – 5117Combined sources
Beta strandi513 – 5197Combined sources
Beta strandi522 – 5243Combined sources
Turni532 – 5354Combined sources
Helixi536 – 5383Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XOCX-ray1.55A25-543[»]
ProteinModelPortaliP42061.
SMRiP42061. Positions 40-543.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42061.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiP42061.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRRKTALMM LSVLMVLAIF LSACSGSKSS NSSAKKSAGK PQQGGDLVVG
60 70 80 90 100
SIGEPTLFNS LYSTDDASTD IENMLYSFLT KTDEKLNVKL SLAESIKELD
110 120 130 140 150
GGLAYDVKIK KGVKFHDGKE LTADDVVFTY SVPLSKDYKG ERGSTYEMLK
160 170 180 190 200
SVEKKGDYEV LFKLKYKDGN FYNNALDSTA ILPKHILGNV PIADLEENEF
210 220 230 240 250
NRKKPIGSGP FKFKEWKQGQ YIKLEANDDY FEGRPYLDTV TYKVIPDANA
260 270 280 290 300
AVAQLQAGDI NFFNVPATDY KTAEKFNNLK IVTDLALSYV YIGWNEKNEL
310 320 330 340 350
FKDKKVRQAL TTALDRESIV SQVLDGDGEV AYIPESPLSW NYPKDIDVPK
360 370 380 390 400
FEYNEKKAKQ MLAEAGWKDT NGDGILDKDG KKFSFTLKTN QGNKVREDIA
410 420 430 440 450
VVVQEQLKKI GIEVKTQIVE WSALVEQMNP PNWDFDAMVM GWSLSTFPDQ
460 470 480 490 500
YDIFHSSQIK KGLNYVWYKN AEADKLMKDA KSISDRKQYS KEYEQIYQKI
510 520 530 540
AEDQPYTFLY YPNNHMAMPE NLEGYKYHPK RDLYNIEKWW LAK
Length:543
Mass (Da):61,917
Last modified:November 3, 2009 - v2
Checksum:i87E465405D36975B
GO

Sequence cautioni

The sequence AL009126 differs from that shown. Reason: Frameshift at position 156. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 2521V → E in AAA62358 (PubMed:7997159).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20909 Genomic DNA. Translation: AAA62358.1.
AL009126 Genomic DNA. No translation available.
PIRiI40545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20909 Genomic DNA. Translation: AAA62358.1.
AL009126 Genomic DNA. No translation available.
PIRiI40545.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XOCX-ray1.55A25-543[»]
ProteinModelPortaliP42061.
SMRiP42061. Positions 40-543.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.A.1.5.20. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiP42061.

Miscellaneous databases

EvolutionaryTraceiP42061.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPPA_BACSU
AccessioniPrimary (citable) accession number: P42061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 3, 2009
Last modified: February 17, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.