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P42061 (APPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Oligopeptide-binding protein AppA
Gene names
Name:appA
Ordered Locus Names:BSU11381/BSU11382
ORF Names:BSU11380
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapeptides but not tripeptides.

Subcellular location

Cell membrane; Lipid-anchor Probable.

Sequence similarities

Belongs to the bacterial solute-binding protein 5 family.

Sequence caution

The sequence AL009126 differs from that shown. Reason: Frameshift at position 156.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 543520Oligopeptide-binding protein AppA
PRO_0000031791

Amino acid modifications

Lipidation241N-palmitoyl cysteine Probable
Lipidation241S-diacylglycerol cysteine Probable

Experimental info

Sequence conflict2521V → E in AAA62358. Ref.1

Secondary structure

1 ................................................................................................ 543
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P42061 [UniParc].

Last modified November 3, 2009. Version 2.
Checksum: 87E465405D36975B

FASTA54361,917
        10         20         30         40         50         60 
MKRRKTALMM LSVLMVLAIF LSACSGSKSS NSSAKKSAGK PQQGGDLVVG SIGEPTLFNS 

        70         80         90        100        110        120 
LYSTDDASTD IENMLYSFLT KTDEKLNVKL SLAESIKELD GGLAYDVKIK KGVKFHDGKE 

       130        140        150        160        170        180 
LTADDVVFTY SVPLSKDYKG ERGSTYEMLK SVEKKGDYEV LFKLKYKDGN FYNNALDSTA 

       190        200        210        220        230        240 
ILPKHILGNV PIADLEENEF NRKKPIGSGP FKFKEWKQGQ YIKLEANDDY FEGRPYLDTV 

       250        260        270        280        290        300 
TYKVIPDANA AVAQLQAGDI NFFNVPATDY KTAEKFNNLK IVTDLALSYV YIGWNEKNEL 

       310        320        330        340        350        360 
FKDKKVRQAL TTALDRESIV SQVLDGDGEV AYIPESPLSW NYPKDIDVPK FEYNEKKAKQ 

       370        380        390        400        410        420 
MLAEAGWKDT NGDGILDKDG KKFSFTLKTN QGNKVREDIA VVVQEQLKKI GIEVKTQIVE 

       430        440        450        460        470        480 
WSALVEQMNP PNWDFDAMVM GWSLSTFPDQ YDIFHSSQIK KGLNYVWYKN AEADKLMKDA 

       490        500        510        520        530        540 
KSISDRKQYS KEYEQIYQKI AEDQPYTFLY YPNNHMAMPE NLEGYKYHPK RDLYNIEKWW 


LAK

« Hide

References

« Hide 'large scale' references
[1]"Identification of a second oligopeptide transport system in Bacillus subtilis and determination of its role in sporulation."
Koide A., Hoch J.A.
Mol. Microbiol. 13:417-426(1994) [PubMed: 7997159] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U20909 Genomic DNA. Translation: AAA62358.1.
AL009126 Genomic DNA. No translation available.
PIRI40545.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1XOCX-ray1.55A25-543[»]
ProteinModelPortalP42061.
SMRP42061. Positions 40-543.
ModBaseSearch...

Protein family/group databases

TCDB3.A.1.5.20. ATP-binding cassette (ABC) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GenomeReviewsGene locus appA in contig AL009126_GR.
NMPDRfig|224308.1.peg.1139.

Organism-specific databases

GenoListBSU11381. [Micado]
BSU11382. [Micado]

Phylogenomic databases

HOGENOMHBG567744.
PhylomeDBP42061.

Family and domain databases

InterProIPR000914. SBP_bac_5.
IPR023765. SBP_bac_5_CS.
[Graphical view]
PfamPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PROSITEPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPPA_BACSU
AccessionPrimary (citable) accession number: P42061
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 3, 2009
Last modified: September 21, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents