Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Oligopeptide-binding protein AppA

Gene

appA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapeptides but not tripeptides.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Competence, Peptide transport, Protein transport, Sporulation, Transport

Protein family/group databases

TCDBi3.A.1.5.20. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligopeptide-binding protein AppA
Gene namesi
Name:appA
Ordered Locus Names:BSU11381/BSU11382
ORF Names:BSU11380
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23PROSITE-ProRule annotationAdd BLAST23
ChainiPRO_000003179124 – 543Oligopeptide-binding protein AppAAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi24N-palmitoyl cysteineCurated1
Lipidationi24S-diacylglycerol cysteineCurated1

Keywords - PTMi

Lipoprotein, Palmitate

Structurei

Secondary structure

1543
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi46 – 53Combined sources8
Turni60 – 62Combined sources3
Helixi66 – 75Combined sources10
Beta strandi79 – 82Combined sources4
Beta strandi88 – 99Combined sources12
Helixi100 – 102Combined sources3
Beta strandi104 – 109Combined sources6
Helixi123 – 131Combined sources9
Helixi132 – 134Combined sources3
Helixi143 – 145Combined sources3
Turni146 – 148Combined sources3
Beta strandi149 – 156Combined sources8
Beta strandi159 – 166Combined sources8
Helixi170 – 173Combined sources4
Helixi175 – 178Combined sources4
Helixi184 – 187Combined sources4
Helixi192 – 194Combined sources3
Turni195 – 197Combined sources3
Helixi199 – 201Combined sources3
Beta strandi208 – 217Combined sources10
Turni218 – 220Combined sources3
Beta strandi221 – 226Combined sources6
Beta strandi236 – 244Combined sources9
Helixi248 – 256Combined sources9
Beta strandi262 – 264Combined sources3
Helixi267 – 269Combined sources3
Helixi270 – 273Combined sources4
Beta strandi279 – 285Combined sources7
Beta strandi287 – 294Combined sources8
Helixi299 – 301Combined sources3
Helixi304 – 312Combined sources9
Helixi316 – 323Combined sources8
Beta strandi328 – 330Combined sources3
Beta strandi333 – 335Combined sources3
Helixi355 – 364Combined sources10
Beta strandi370 – 375Combined sources6
Beta strandi384 – 390Combined sources7
Helixi394 – 408Combined sources15
Turni409 – 411Combined sources3
Beta strandi413 – 419Combined sources7
Helixi421 – 428Combined sources8
Turni430 – 432Combined sources3
Beta strandi436 – 443Combined sources8
Helixi451 – 454Combined sources4
Helixi456 – 458Combined sources3
Turni459 – 461Combined sources3
Helixi471 – 480Combined sources10
Helixi486 – 503Combined sources18
Beta strandi505 – 511Combined sources7
Beta strandi513 – 519Combined sources7
Beta strandi522 – 524Combined sources3
Turni532 – 535Combined sources4
Helixi536 – 538Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XOCX-ray1.55A25-543[»]
ProteinModelPortaliP42061.
SMRiP42061.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42061.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiP42061.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRRKTALMM LSVLMVLAIF LSACSGSKSS NSSAKKSAGK PQQGGDLVVG
60 70 80 90 100
SIGEPTLFNS LYSTDDASTD IENMLYSFLT KTDEKLNVKL SLAESIKELD
110 120 130 140 150
GGLAYDVKIK KGVKFHDGKE LTADDVVFTY SVPLSKDYKG ERGSTYEMLK
160 170 180 190 200
SVEKKGDYEV LFKLKYKDGN FYNNALDSTA ILPKHILGNV PIADLEENEF
210 220 230 240 250
NRKKPIGSGP FKFKEWKQGQ YIKLEANDDY FEGRPYLDTV TYKVIPDANA
260 270 280 290 300
AVAQLQAGDI NFFNVPATDY KTAEKFNNLK IVTDLALSYV YIGWNEKNEL
310 320 330 340 350
FKDKKVRQAL TTALDRESIV SQVLDGDGEV AYIPESPLSW NYPKDIDVPK
360 370 380 390 400
FEYNEKKAKQ MLAEAGWKDT NGDGILDKDG KKFSFTLKTN QGNKVREDIA
410 420 430 440 450
VVVQEQLKKI GIEVKTQIVE WSALVEQMNP PNWDFDAMVM GWSLSTFPDQ
460 470 480 490 500
YDIFHSSQIK KGLNYVWYKN AEADKLMKDA KSISDRKQYS KEYEQIYQKI
510 520 530 540
AEDQPYTFLY YPNNHMAMPE NLEGYKYHPK RDLYNIEKWW LAK
Length:543
Mass (Da):61,917
Last modified:November 3, 2009 - v2
Checksum:i87E465405D36975B
GO

Sequence cautioni

The sequence AL009126 differs from that shown. Reason: Frameshift at position 156.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252V → E in AAA62358 (PubMed:7997159).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20909 Genomic DNA. Translation: AAA62358.1.
AL009126 Genomic DNA. No translation available.
PIRiI40545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20909 Genomic DNA. Translation: AAA62358.1.
AL009126 Genomic DNA. No translation available.
PIRiI40545.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XOCX-ray1.55A25-543[»]
ProteinModelPortaliP42061.
SMRiP42061.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.A.1.5.20. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiP42061.

Miscellaneous databases

EvolutionaryTraceiP42061.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPPA_BACSU
AccessioniPrimary (citable) accession number: P42061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 3, 2009
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.