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P42043 (HEMH1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ferrochelatase-1, chloroplastic/mitochondrial
EC=4.99.1.1
Alternative name(s):
Ferrochelatase I
Heme synthase 1
Protoheme ferro-lyase 1
Gene names
Name:HEM15
Ordered Locus Names:At5g26030
ORF Names:T1N24.17
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the ferrous insertion into protoporphyrin IX. May have a role in dealing with oxidative stress.

Catalytic activity

Protoheme + 2 H+ = protoporphyrin + Fe2+.

Pathway

Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.

Subcellular location

Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast thylakoid membrane; Peripheral membrane protein. Mitochondrion Ref.5 Ref.6.

Tissue specificity

Present in both leaves and roots.

Sequence similarities

Belongs to the ferrochelatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast and mitochondrion Potential
Chain? – 466Ferrochelatase-1, chloroplastic/mitochondrialPRO_0000008880

Sequences

Sequence LengthMass (Da)Tools
P42043 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: BAB2F960A5AEBB6A

FASTA46652,033
        10         20         30         40         50         60 
MQATALSSGF NPLTKRKDHR FPRSCSQRNS LSLIQCDIKE RSFGESMTIT NRGLSFKTNV 

        70         80         90        100        110        120 
FEQARSVTGD CSYDETSAKA RSHVVAEDKI GVLLLNLGGP ETLNDVQPFL YNLFADPDII 

       130        140        150        160        170        180 
RLPRPFQFLQ GTIAKFISVV RAPKSKEGYA AIGGGSPLRK ITDEQADAIK MSLQAKNIAA 

       190        200        210        220        230        240 
NVYVGMRYWY PFTEEAVQQI KKDKITRLVV LPLYPQYSIS TTGSSIRVLQ DLFRKDPYLA 

       250        260        270        280        290        300 
GVPVAIIKSW YQRRGYVNSM ADLIEKELQT FSDPKEVMIF FSAHGVPVSY VENAGDPYQK 

       310        320        330        340        350        360 
QMEECIDLIM EELKARGVLN DHKLAYQSRV GPVQWLKPYT DEVLVDLGKS GVKSLLAVPV 

       370        380        390        400        410        420 
SFVSEHIETL EEIDMEYREL ALESGVENWG RVPALGLTPS FITDLADAVI ESLPSAEAMS 

       430        440        450        460 
NPNAVVDSED SESSDAFSYI VKMFFGSILA FVLLLSPKMF HAFRNL

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a cDNA encoding chloroplast ferrochelatase from Arabidopsis thaliana by functional complementation of a yeast mutant."
Smith A.G., Santana M.A., Wallace-Cook A.D.M., Roper J.M., Labbe-Bois R.
J. Biol. Chem. 269:13405-13413(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Landsberg erecta.
Tissue: Seedling.
[2]"Two different genes encode ferrochelatase in Arabidopsis: mapping, expression and subcellular targeting of the precursor proteins."
Chow K.-S., Singh D.P., Walker A., Smith A.G.
Plant J. 15:531-541(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[3]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Molecular localisation of ferrochelatase in higher plant chloroplasts."
Roper J.M., Smith A.G.
Eur. J. Biochem. 246:32-37(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"A single precursor protein for ferrochelatase-I from Arabidopsis is imported in vitro into both chloroplasts and mitochondria."
Chow K.-S., Singh D.P., Roper J.M., Smith A.G.
J. Biol. Chem. 272:27565-27571(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X73417 mRNA. Translation: CAA51819.1.
Y13382 Genomic DNA. Translation: CAA73809.1.
AF149413 Genomic DNA. Translation: AAD40138.1.
CP002688 Genomic DNA. Translation: AED93514.1.
CP002688 Genomic DNA. Translation: AED93515.1.
IPIIPI00539854.
PIRA54125.
RefSeqNP_001031941.1. NM_001036864.1.
NP_197975.3. NM_122504.4.
UniGeneAt.58.
At.70191.

3D structure databases

ProteinModelPortalP42043.
SMRP42043. Positions 89-420.
ModBaseSearch...

Proteomic databases

PaxDbP42043.
PRIDEP42043.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G26030.1; AT5G26030.1; AT5G26030.
AT5G26030.2; AT5G26030.2; AT5G26030.
GeneID832672.
KEGGath:AT5G26030.

Organism-specific databases

TAIRAt5g26030.

Phylogenomic databases

eggNOGCOG0276.
HOGENOMHOG000060727.
InParanoidP42043.
KOK01772.
OMALLLSPKM.
PhylomeDBP42043.
ProtClustDBPLN02449.

Enzyme and pathway databases

BioCycARA:AT5G26030-MONOMER.
UniPathwayUPA00252; UER00325.

Gene expression databases

ArrayExpressP42043.
GenevestigatorP42043.
GermOnlineAT5G26030. Arabidopsis thaliana.

Family and domain databases

InterProIPR001015. Ferrochelatase.
IPR019772. Ferrochelatase_AS.
[Graphical view]
PANTHERPTHR11108. PTHR11108. 1 hit.
PfamPF00762. Ferrochelatase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00109. hemH. 1 hit.
PROSITEPS00534. FERROCHELATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEMH1_ARATH
AccessionPrimary (citable) accession number: P42043
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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