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P42043

- HEMH1_ARATH

UniProt

P42043 - HEMH1_ARATH

Protein

Ferrochelatase-1, chloroplastic/mitochondrial

Gene

HEM15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the ferrous insertion into protoporphyrin IX. May have a role in dealing with oxidative stress. May be involved in wound-induced supply of heme to defensive hemoproteins outside plastids.1 Publication

    Catalytic activityi

    Protoheme + 2 H+ = protoporphyrin + Fe2+.

    Pathwayi

    GO - Molecular functioni

    1. ferrochelatase activity Source: TAIR

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
    2. heme biosynthetic process Source: TAIR
    3. response to oxidative stress Source: TAIR
    4. tetrapyrrole biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Iron

    Enzyme and pathway databases

    BioCyciARA:AT5G26030-MONOMER.
    ARA:GQT-1215-MONOMER.
    UniPathwayiUPA00668.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ferrochelatase-1, chloroplastic/mitochondrial (EC:4.99.1.1)
    Short name:
    AtFC1
    Alternative name(s):
    Ferrochelatase I
    Heme synthase 1
    Protoheme ferro-lyase 1
    Gene namesi
    Name:HEM15
    Ordered Locus Names:At5g26030
    ORF Names:T1N24.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G26030.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast membrane Source: UniProtKB-SubCell
    3. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    4. mitochondrion Source: TAIR
    5. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Membrane, Mitochondrion, Plastid, Thylakoid

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype, but decreased heme content in roots.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3535Chloroplast and mitochondrionSequence AnalysisAdd
    BLAST
    Chaini36 – 466431Ferrochelatase-1, chloroplastic/mitochondrialPRO_0000008880Add
    BLAST

    Proteomic databases

    PaxDbiP42043.
    PRIDEiP42043.

    Expressioni

    Tissue specificityi

    Present in both leaves and roots.1 Publication

    Inductioni

    Up-regulated by wounding and reactive oxygen species. Not regulated by methyl jasmonate.1 Publication

    Gene expression databases

    GenevestigatoriP42043.

    Structurei

    3D structure databases

    ProteinModelPortaliP42043.
    SMRiP42043. Positions 89-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ferrochelatase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0276.
    HOGENOMiHOG000060727.
    InParanoidiP42043.
    KOiK01772.
    OMAiESGVENW.
    PhylomeDBiP42043.

    Family and domain databases

    HAMAPiMF_00323. Ferrochelatase.
    InterProiIPR001015. Ferrochelatase.
    IPR019772. Ferrochelatase_AS.
    [Graphical view]
    PANTHERiPTHR11108. PTHR11108. 1 hit.
    PfamiPF00762. Ferrochelatase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00109. hemH. 1 hit.
    PROSITEiPS00534. FERROCHELATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42043-1 [UniParc]FASTAAdd to Basket

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    MQATALSSGF NPLTKRKDHR FPRSCSQRNS LSLIQCDIKE RSFGESMTIT    50
    NRGLSFKTNV FEQARSVTGD CSYDETSAKA RSHVVAEDKI GVLLLNLGGP 100
    ETLNDVQPFL YNLFADPDII RLPRPFQFLQ GTIAKFISVV RAPKSKEGYA 150
    AIGGGSPLRK ITDEQADAIK MSLQAKNIAA NVYVGMRYWY PFTEEAVQQI 200
    KKDKITRLVV LPLYPQYSIS TTGSSIRVLQ DLFRKDPYLA GVPVAIIKSW 250
    YQRRGYVNSM ADLIEKELQT FSDPKEVMIF FSAHGVPVSY VENAGDPYQK 300
    QMEECIDLIM EELKARGVLN DHKLAYQSRV GPVQWLKPYT DEVLVDLGKS 350
    GVKSLLAVPV SFVSEHIETL EEIDMEYREL ALESGVENWG RVPALGLTPS 400
    FITDLADAVI ESLPSAEAMS NPNAVVDSED SESSDAFSYI VKMFFGSILA 450
    FVLLLSPKMF HAFRNL 466
    Length:466
    Mass (Da):52,033
    Last modified:November 1, 1995 - v1
    Checksum:iBAB2F960A5AEBB6A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73417 mRNA. Translation: CAA51819.1.
    Y13382 Genomic DNA. Translation: CAA73809.1.
    AF149413 Genomic DNA. Translation: AAD40138.1.
    CP002688 Genomic DNA. Translation: AED93514.1.
    CP002688 Genomic DNA. Translation: AED93515.1.
    PIRiA54125.
    RefSeqiNP_001031941.1. NM_001036864.1.
    NP_197975.3. NM_122504.4.
    UniGeneiAt.58.
    At.70191.

    Genome annotation databases

    EnsemblPlantsiAT5G26030.1; AT5G26030.1; AT5G26030.
    AT5G26030.2; AT5G26030.2; AT5G26030.
    GeneIDi832672.
    KEGGiath:AT5G26030.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73417 mRNA. Translation: CAA51819.1 .
    Y13382 Genomic DNA. Translation: CAA73809.1 .
    AF149413 Genomic DNA. Translation: AAD40138.1 .
    CP002688 Genomic DNA. Translation: AED93514.1 .
    CP002688 Genomic DNA. Translation: AED93515.1 .
    PIRi A54125.
    RefSeqi NP_001031941.1. NM_001036864.1.
    NP_197975.3. NM_122504.4.
    UniGenei At.58.
    At.70191.

    3D structure databases

    ProteinModelPortali P42043.
    SMRi P42043. Positions 89-420.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P42043.
    PRIDEi P42043.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G26030.1 ; AT5G26030.1 ; AT5G26030 .
    AT5G26030.2 ; AT5G26030.2 ; AT5G26030 .
    GeneIDi 832672.
    KEGGi ath:AT5G26030.

    Organism-specific databases

    TAIRi AT5G26030.

    Phylogenomic databases

    eggNOGi COG0276.
    HOGENOMi HOG000060727.
    InParanoidi P42043.
    KOi K01772.
    OMAi ESGVENW.
    PhylomeDBi P42043.

    Enzyme and pathway databases

    UniPathwayi UPA00668 .
    BioCyci ARA:AT5G26030-MONOMER.
    ARA:GQT-1215-MONOMER.

    Miscellaneous databases

    PROi P42043.

    Gene expression databases

    Genevestigatori P42043.

    Family and domain databases

    HAMAPi MF_00323. Ferrochelatase.
    InterProi IPR001015. Ferrochelatase.
    IPR019772. Ferrochelatase_AS.
    [Graphical view ]
    PANTHERi PTHR11108. PTHR11108. 1 hit.
    Pfami PF00762. Ferrochelatase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00109. hemH. 1 hit.
    PROSITEi PS00534. FERROCHELATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a cDNA encoding chloroplast ferrochelatase from Arabidopsis thaliana by functional complementation of a yeast mutant."
      Smith A.G., Santana M.A., Wallace-Cook A.D.M., Roper J.M., Labbe-Bois R.
      J. Biol. Chem. 269:13405-13413(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Landsberg erecta.
      Tissue: Seedling.
    2. "Two different genes encode ferrochelatase in Arabidopsis: mapping, expression and subcellular targeting of the precursor proteins."
      Chow K.-S., Singh D.P., Walker A., Smith A.G.
      Plant J. 15:531-541(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Landsberg erecta.
    3. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Molecular localisation of ferrochelatase in higher plant chloroplasts."
      Roper J.M., Smith A.G.
      Eur. J. Biochem. 246:32-37(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "A single precursor protein for ferrochelatase-I from Arabidopsis is imported in vitro into both chloroplasts and mitochondria."
      Chow K.-S., Singh D.P., Roper J.M., Smith A.G.
      J. Biol. Chem. 272:27565-27571(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Induction of isoforms of tetrapyrrole biosynthetic enzymes, AtHEMA2 and AtFC1, under stress conditions and their physiological functions in Arabidopsis."
      Nagai S., Koide M., Takahashi S., Kikuta A., Aono M., Sasaki-Sekimoto Y., Ohta H., Takamiya K., Masuda T.
      Plant Physiol. 144:1039-1051(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY WOUNDING AND REACTIVE OXYGEN SPECIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiHEMH1_ARATH
    AccessioniPrimary (citable) accession number: P42043
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3