Glucoamylase precursor - Arxula adeninivorans (Yeast)

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Reviewed, UniProtKB/Swiss-Prot P42042 (AMYG_ARXAD)

Last modified June 16, 2009. Version 52. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Glucoamylase
    EC=3.2.1.3
Alternative name(s):
    Glucan 1,4-alpha-glucosidase
    1,4-alpha-D-glucan glucohydrolase

Gene names

Name:

GAA

Organism

Arxula adeninivorans (Yeast)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › mitosporic Dipodascaceae › Arxula

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 15 family.

Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucan 1,4-alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

18

Potential

Chain

606

Glucoamylase

PRO_0000001458

Regions

Domain

107

CBM21

Sites

Active site

1

Proton acceptor By similarity

Active site

1

Proton donor By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P42042-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 74C2CDFB43FF71B4
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624

68,981

        10         20         30         40         50         60 
MRQFLALAAA ASIAVADSCH TFTLANSPPD DKAVALSSYS YCGGYLSASA FVKNLSYDKL 

        70         80         90        100        110        120 
VTLYWTNADN KSTPLNAGSL DYVKAASDDQ SWELWSLNVT TVPDGVDALL NITYVAASIG 

       130        140        150        160        170        180 
KTNSQQLNVQ VEATGDPIPT PQIPTIYKPY ASPSDFSDDI TNWLKPSNDS QTGIAKSFLF 

       190        200        210        220        230        240 
NNINIPGAAP GTVIAAQSYS EPDYAYTWVR DASLVMDVVN RLYSSAKSEE KRQLYEKILF 

       250        260        270        280        290        300 
QYAKAGAQEQ NDPTAISGMG EPKFYLNNTA FTGSWGRPQN DGPATRAITL IEFANAYLAN 

       310        320        330        340        350        360 
GGSQDTVREQ LYDSDKYPQV APIKKDLQFV ASNWSSPSFD LWEEEESAHF YTRLVQRKAL 

       370        380        390        400        410        420 
LLGADFANDM GDHELSDKLK TQASKLSDTL PEFWDSARQL ILYEYGPVLR GKYSYKDISV 

       430        440        450        460        470        480 
VLGVMHGYAN DNVFSYTNDQ ILATAYQVST SFLDVYKVAN TTSDESGKPL GIPVGRYPED 

       490        500        510        520        530        540 
VYDGVGTSQG NPWYLTTMAM AEFLYRSVQE FEDAGSIIIS DTSLPFWKYF ASSVDHKAGA 

       550        560        570        580        590        600 
KYNKNDQSFK TSLKSLTGWG DAFMRRAKYH TPSSGHMSEE FNRTTGEPRG AKDLTWSYAS 

       610        620 
LLSAAFAREE LRNQKNYLTN VADL

References

[1]	"Cloning and expression of an Arxula adeninivorans glucoamylase gene in Saccharomyces cerevisiae." Bui Minh D., Kunze I., Foerster S., Wartmann T., Horstmann C., Manteuffel R., Kunze G. Appl. Microbiol. Biotechnol. 44:610-619(1996) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LS3.

Cross-references

Sequence databases
	Z46901 Genomic DNA. Translation: CAA86997.1.
3D structure databases
HSSP	HSSP built from PDB template 1GAI based on UniProtKB P04064.
ModBase	Search...
Protein family/group databases
CAZy	CBM21. Carbohydrate-Binding Module Family 21. GH15. Glycoside Hydrolase Family 15.
Enzyme and pathway databases
BRENDA	3.2.1.3. 97311.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR005036. CBM_21. IPR000165. Glyco_hydro_15. IPR011613. Glyco_hydro_15_rel. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
Pfam	PF03370. CBM_21. 1 hit. PF00723. Glyco_hydro_15. 1 hit. [Graphical view]
PRINTS	PR00736. GLHYDRLASE15.
PROSITE	PS51159. CBM21. 1 hit. PS00820. GLUCOAMYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYG_ARXAD

Accession

Primary (citable) accession number: P42042

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents