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P42042 (AMYG_BLAAD) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucoamylase

EC=3.2.1.3
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene names
Name:GAA
OrganismBlastobotrys adeninivorans (Yeast) (Arxula adeninivorans)
Taxonomic identifier409370 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesBlastobotrys

Protein attributes

Sequence length624 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 15 family.

Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionglucan 1,4-alpha-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 624606Glucoamylase
PRO_0000001458

Regions

Domain26 – 132107CBM21

Sites

Active site3401Proton acceptor By similarity
Active site3431Proton donor By similarity

Amino acid modifications

Glycosylation541N-linked (GlcNAc...) Potential
Glycosylation701N-linked (GlcNAc...) Potential
Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1111N-linked (GlcNAc...) Potential
Glycosylation1681N-linked (GlcNAc...) Potential
Glycosylation2671N-linked (GlcNAc...) Potential
Glycosylation3331N-linked (GlcNAc...) Potential
Glycosylation4601N-linked (GlcNAc...) Potential
Glycosylation5821N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P42042 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 74C2CDFB43FF71B4

FASTA62468,981
        10         20         30         40         50         60 
MRQFLALAAA ASIAVADSCH TFTLANSPPD DKAVALSSYS YCGGYLSASA FVKNLSYDKL 

        70         80         90        100        110        120 
VTLYWTNADN KSTPLNAGSL DYVKAASDDQ SWELWSLNVT TVPDGVDALL NITYVAASIG 

       130        140        150        160        170        180 
KTNSQQLNVQ VEATGDPIPT PQIPTIYKPY ASPSDFSDDI TNWLKPSNDS QTGIAKSFLF 

       190        200        210        220        230        240 
NNINIPGAAP GTVIAAQSYS EPDYAYTWVR DASLVMDVVN RLYSSAKSEE KRQLYEKILF 

       250        260        270        280        290        300 
QYAKAGAQEQ NDPTAISGMG EPKFYLNNTA FTGSWGRPQN DGPATRAITL IEFANAYLAN 

       310        320        330        340        350        360 
GGSQDTVREQ LYDSDKYPQV APIKKDLQFV ASNWSSPSFD LWEEEESAHF YTRLVQRKAL 

       370        380        390        400        410        420 
LLGADFANDM GDHELSDKLK TQASKLSDTL PEFWDSARQL ILYEYGPVLR GKYSYKDISV 

       430        440        450        460        470        480 
VLGVMHGYAN DNVFSYTNDQ ILATAYQVST SFLDVYKVAN TTSDESGKPL GIPVGRYPED 

       490        500        510        520        530        540 
VYDGVGTSQG NPWYLTTMAM AEFLYRSVQE FEDAGSIIIS DTSLPFWKYF ASSVDHKAGA 

       550        560        570        580        590        600 
KYNKNDQSFK TSLKSLTGWG DAFMRRAKYH TPSSGHMSEE FNRTTGEPRG AKDLTWSYAS 

       610        620 
LLSAAFAREE LRNQKNYLTN VADL 

« Hide

References

[1]"Cloning and expression of an Arxula adeninivorans glucoamylase gene in Saccharomyces cerevisiae."
Bui Minh D., Kunze I., Foerster S., Wartmann T., Horstmann C., Manteuffel R., Kunze G.
Appl. Microbiol. Biotechnol. 44:610-619(1996)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LS3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46901 Genomic DNA. Translation: CAA86997.1.

3D structure databases

ProteinModelPortalP42042.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM21. Carbohydrate-Binding Module Family 21.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPGLA15A_BLAAD.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005036. CBM_21.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamPF03370. CBM_21. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSPR00736. GLHYDRLASE15.
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS51159. CBM21. 1 hit.
PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMYG_BLAAD
AccessionPrimary (citable) accession number: P42042
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 16, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries