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P42042

- AMYG_BLAAD

UniProt

P42042 - AMYG_BLAAD

Protein

Glucoamylase

Gene

GAA

Organism
Blastobotrys adeninivorans (Yeast) (Arxula adeninivorans)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei340 – 3401Proton acceptorPROSITE-ProRule annotation
    Active sitei343 – 3431Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM21. Carbohydrate-Binding Module Family 21.
    GH15. Glycoside Hydrolase Family 15.
    mycoCLAPiGLA15A_BLAAD.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucoamylase (EC:3.2.1.3)
    Alternative name(s):
    1,4-alpha-D-glucan glucohydrolase
    Glucan 1,4-alpha-glucosidase
    Gene namesi
    Name:GAA
    OrganismiBlastobotrys adeninivorans (Yeast) (Arxula adeninivorans)
    Taxonomic identifieri409370 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesBlastobotrys

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 624606GlucoamylasePRO_0000001458Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi70 – 701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi582 – 5821N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliP42042.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 132107CBM21PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 15 family.Curated
    Contains 1 CBM21 (carbohydrate binding type-21) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    PhylomeDBiP42042.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR005036. CBM_21.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view]
    PfamiPF03370. CBM_21. 1 hit.
    PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    PRINTSiPR00736. GLHYDRLASE15.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS51159. CBM21. 1 hit.
    PS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42042-1 [UniParc]FASTAAdd to Basket

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    MRQFLALAAA ASIAVADSCH TFTLANSPPD DKAVALSSYS YCGGYLSASA    50
    FVKNLSYDKL VTLYWTNADN KSTPLNAGSL DYVKAASDDQ SWELWSLNVT 100
    TVPDGVDALL NITYVAASIG KTNSQQLNVQ VEATGDPIPT PQIPTIYKPY 150
    ASPSDFSDDI TNWLKPSNDS QTGIAKSFLF NNINIPGAAP GTVIAAQSYS 200
    EPDYAYTWVR DASLVMDVVN RLYSSAKSEE KRQLYEKILF QYAKAGAQEQ 250
    NDPTAISGMG EPKFYLNNTA FTGSWGRPQN DGPATRAITL IEFANAYLAN 300
    GGSQDTVREQ LYDSDKYPQV APIKKDLQFV ASNWSSPSFD LWEEEESAHF 350
    YTRLVQRKAL LLGADFANDM GDHELSDKLK TQASKLSDTL PEFWDSARQL 400
    ILYEYGPVLR GKYSYKDISV VLGVMHGYAN DNVFSYTNDQ ILATAYQVST 450
    SFLDVYKVAN TTSDESGKPL GIPVGRYPED VYDGVGTSQG NPWYLTTMAM 500
    AEFLYRSVQE FEDAGSIIIS DTSLPFWKYF ASSVDHKAGA KYNKNDQSFK 550
    TSLKSLTGWG DAFMRRAKYH TPSSGHMSEE FNRTTGEPRG AKDLTWSYAS 600
    LLSAAFAREE LRNQKNYLTN VADL 624
    Length:624
    Mass (Da):68,981
    Last modified:November 1, 1995 - v1
    Checksum:i74C2CDFB43FF71B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46901 Genomic DNA. Translation: CAA86997.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46901 Genomic DNA. Translation: CAA86997.1 .

    3D structure databases

    ProteinModelPortali P42042.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM21. Carbohydrate-Binding Module Family 21.
    GH15. Glycoside Hydrolase Family 15.
    mycoCLAPi GLA15A_BLAAD.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    PhylomeDBi P42042.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR005036. CBM_21.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view ]
    Pfami PF03370. CBM_21. 1 hit.
    PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    PRINTSi PR00736. GLHYDRLASE15.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS51159. CBM21. 1 hit.
    PS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of an Arxula adeninivorans glucoamylase gene in Saccharomyces cerevisiae."
      Bui Minh D., Kunze I., Foerster S., Wartmann T., Horstmann C., Manteuffel R., Kunze G.
      Appl. Microbiol. Biotechnol. 44:610-619(1996)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LS3.

    Entry informationi

    Entry nameiAMYG_BLAAD
    AccessioniPrimary (citable) accession number: P42042
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3