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Protein

Glucoamylase

Gene

GAA

Organism
Blastobotrys adeninivorans (Yeast) (Arxula adeninivorans)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei340Proton acceptorPROSITE-ProRule annotation1
Active sitei343Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPiGLA15A_ARXAD.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GAA
OrganismiBlastobotrys adeninivorans (Yeast) (Arxula adeninivorans)
Taxonomic identifieri409370 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesTrichomonascaceaeBlastobotrys

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000000145819 – 624GlucoamylaseAdd BLAST606

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi54N-linked (GlcNAc...)Sequence analysis1
Glycosylationi70N-linked (GlcNAc...)Sequence analysis1
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Glycosylationi111N-linked (GlcNAc...)Sequence analysis1
Glycosylationi168N-linked (GlcNAc...)Sequence analysis1
Glycosylationi267N-linked (GlcNAc...)Sequence analysis1
Glycosylationi333N-linked (GlcNAc...)Sequence analysis1
Glycosylationi460N-linked (GlcNAc...)Sequence analysis1
Glycosylationi582N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP42042.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 132CBM21PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated
Contains 1 CBM21 (carbohydrate binding type-21) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

PhylomeDBiP42042.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005036. CBM21_dom.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQFLALAAA ASIAVADSCH TFTLANSPPD DKAVALSSYS YCGGYLSASA
60 70 80 90 100
FVKNLSYDKL VTLYWTNADN KSTPLNAGSL DYVKAASDDQ SWELWSLNVT
110 120 130 140 150
TVPDGVDALL NITYVAASIG KTNSQQLNVQ VEATGDPIPT PQIPTIYKPY
160 170 180 190 200
ASPSDFSDDI TNWLKPSNDS QTGIAKSFLF NNINIPGAAP GTVIAAQSYS
210 220 230 240 250
EPDYAYTWVR DASLVMDVVN RLYSSAKSEE KRQLYEKILF QYAKAGAQEQ
260 270 280 290 300
NDPTAISGMG EPKFYLNNTA FTGSWGRPQN DGPATRAITL IEFANAYLAN
310 320 330 340 350
GGSQDTVREQ LYDSDKYPQV APIKKDLQFV ASNWSSPSFD LWEEEESAHF
360 370 380 390 400
YTRLVQRKAL LLGADFANDM GDHELSDKLK TQASKLSDTL PEFWDSARQL
410 420 430 440 450
ILYEYGPVLR GKYSYKDISV VLGVMHGYAN DNVFSYTNDQ ILATAYQVST
460 470 480 490 500
SFLDVYKVAN TTSDESGKPL GIPVGRYPED VYDGVGTSQG NPWYLTTMAM
510 520 530 540 550
AEFLYRSVQE FEDAGSIIIS DTSLPFWKYF ASSVDHKAGA KYNKNDQSFK
560 570 580 590 600
TSLKSLTGWG DAFMRRAKYH TPSSGHMSEE FNRTTGEPRG AKDLTWSYAS
610 620
LLSAAFAREE LRNQKNYLTN VADL
Length:624
Mass (Da):68,981
Last modified:November 1, 1995 - v1
Checksum:i74C2CDFB43FF71B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46901 Genomic DNA. Translation: CAA86997.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46901 Genomic DNA. Translation: CAA86997.1.

3D structure databases

ProteinModelPortaliP42042.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.
GH15. Glycoside Hydrolase Family 15.
mycoCLAPiGLA15A_ARXAD.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

PhylomeDBiP42042.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005036. CBM21_dom.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMYG_BLAAD
AccessioniPrimary (citable) accession number: P42042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.