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Protein

Enolase

Gene

ENO

Organism
Davidiella tassiana (Mycosphaerella tassiana) (Cladosporium herbarum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei159 – 1591SubstrateBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Active sitei211 – 2111Proton donorBy similarity
Metal bindingi246 – 2461MagnesiumBy similarity
Metal bindingi297 – 2971MagnesiumBy similarity
Binding sitei297 – 2971SubstrateBy similarity
Metal bindingi324 – 3241MagnesiumBy similarity
Binding sitei324 – 3241SubstrateBy similarity
Active sitei349 – 3491Proton acceptorBy similarity
Binding sitei400 – 4001SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Allergen Cla h VI
Allergen: Cla h 6
Gene namesi
Name:ENO
Synonyms:CLAH6
OrganismiDavidiella tassiana (Mycosphaerella tassiana) (Cladosporium herbarum)
Taxonomic identifieri29918 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesDothideomycetidaeCapnodialesCladosporiaceaeCladosporium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE. Second most important allergen of C.herbarum in terms of frequency of sensitization.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi141 – 1411K → A or Q: No effect on IgE-binding. 1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei221. Cla h 6.
3205. Cla h 6.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440EnolasePRO_0000134046Add
BLAST

Proteomic databases

PRIDEiP42040.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP42040.
SMRiP42040. Positions 3-434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni120 – 18970IgE-binding determinantAdd
BLAST
Regioni376 – 3794Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the enolase family.Curated

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPISKIHSRY VYDSRGNPTV EVDIVTETGL HRAIVPSGAS TGSHEACELR
60 70 80 90 100
DGDKSKWAGK GVTKAVANVN EIIAPALIKE NLDVKDQAAV DAFLNKLDGT
110 120 130 140 150
TNKTKIGANA ILGVSMAVAK AAAAEKRVPL YAHISDLSGT KKPFVLPVPF
160 170 180 190 200
MNVVNGGSHA GGRLAFQEFM IVPSGAPSFT EAMRQGAEVY QKLKSLTKKR
210 220 230 240 250
YGQSAGNVGD EGGVAPDIQT AEEALDLITD AIEEAGYTGQ IKIAMDVASS
260 270 280 290 300
EFYKADEKKY DLDFKNPDSD KSKWITYEQL ADQYKQLAAK YPIVSIEDPF
310 320 330 340 350
AEDDWEAWSY FYKTSGSDFQ IVGDDLTVTN PEFIKKAIET KACNALLLKV
360 370 380 390 400
NQIGTITEAI NAAKDSFAAG WGVMVSHRSG ETEDVTIADI VVGLRAGQIK
410 420 430 440
TGAPARSERL AKLNQILRIE EELGDKAVYA GDNFRTAINL
Length:440
Mass (Da):47,519
Last modified:December 15, 1998 - v2
Checksum:iFF43316D94740DFB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1751G → A in AAR00929 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78226 mRNA. Translation: CAA55070.1.
U82438 Genomic DNA. Translation: AAR00929.2.
PIRiS43113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78226 mRNA. Translation: CAA55070.1.
U82438 Genomic DNA. Translation: AAR00929.2.
PIRiS43113.

3D structure databases

ProteinModelPortaliP42040.
SMRiP42040. Positions 3-434.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei221. Cla h 6.
3205. Cla h 6.0101.

Proteomic databases

PRIDEiP42040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of major and minor allergens of Alternaria alternata and Cladosporium herbarum."
    Achatz G., Oberkofler H., Lechenauer E., Simon-Nobbe B., Unger A., Kandler D., Ebner C., Prillinger H., Kraft D., Breitenbach M.
    Mol. Immunol. 32:213-227(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
    Strain: 280202-Berlin.
  2. Simon B.
    Submitted (OCT-1998) to UniProtKB
    Cited for: SEQUENCE REVISION TO 38; 285; 286; 427 AND 428.
  3. Simon-Nobbe B., Oberkofler H., Probst G., Breitenbach M., Achatz G.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 280202-Berlin.
    Tissue: Hyphae.
  4. "Enolases are highly conserved fungal allergens."
    Breitenbach M., Simon B., Probst G., Oberkofler H., Ferreira F., Briza P., Achatz G., Unger A., Ebner C., Kraft D., Hirschwehr R.
    Int. Arch. Allergy Immunol. 113:114-117(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALLERGEN.
  5. Cited for: IGE-BINDING, MUTAGENESIS OF LYS-141.
  6. Cited for: IGE-BINDING.
  7. "The allergens of Cladosporium herbarum and Alternaria alternata."
    Breitenbach M., Simon-Nobbe B.
    Chem. Immunol. 81:48-72(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiENO_DAVTA
AccessioniPrimary (citable) accession number: P42040
Secondary accession number(s): Q6LC77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 15, 1998
Last modified: May 11, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.