Reviewed, UniProtKB/Swiss-Prot P42032 (NUOH_RHOCA)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit H
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit H
    NDH-1 subunit H

Gene names

Name:	nuoH
Synonyms:	ndhA

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone. HAMAP MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex Probable. HAMAP MF_01350
Subcellular location	Cellular chromatophore membrane; Multi-pass membrane protein Ref.3 Ref.4.
Disruption phenotype	No functional NADH-quinone oxidoreductase complex. Cells lacking this gene have a nearly normal respiratory growth phenotype on lactate, however they are unable to perform anaerobic photosynthesis. It is suggested that in R.capsulatus this complex may function in reverse flow under physiological conditions. Ref.2
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	NADH-quinone oxidoreductase subunit H HAMAP MF_01350		PRO_0000117531
Regions
	Topological domain	1 – 15	15	Lumenal Ref.4
	Transmembrane	16 – 35	20	1 Probable
	Topological domain	36 – 86	51	Cytoplasmic Ref.4
	Transmembrane	87 – 106	20	2 Probable
	Topological domain	107 – 110	4	Lumenal Ref.4
	Transmembrane	111 – 130	20	3 Probable
	Topological domain	131 – 156	26	Cytoplasmic Ref.4
	Transmembrane	157 – 176	20	4 Probable
	Topological domain	177 – 191	15	Lumenal Ref.4
	Transmembrane	192 – 211	20	5 Probable
	Topological domain	212 – 245	34	Cytoplasmic Ref.4
	Transmembrane	246 – 265	20	6 Probable
	Topological domain	266 – 276	11	Lumenal Ref.4
	Transmembrane	277 – 296	20	7 Probable
	Topological domain	297 – 313	17	Cytoplasmic Ref.4
	Transmembrane	314 – 333	20	8 Probable
	Topological domain	334 – 345	12	Lumenal Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P42032-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5F9E9D640D911854	FASTA	345	37,852
	10 20 30 40 50 60 MADFWATSLG QTLILLAQGL GIIAFVMIGL LLLVWGDRKI WAAVQMRKGP NVVGAFGLLQ 70 80 90 100 110 120 SVADAAKYVF KEIVVPAGVD KPVYFLAPML SLVLALLAWV VVPFNEGWVM ADINVAVLFV 130 140 150 160 170 180 FAVSSLEVYG VIMGGWASNS KYPFLGSLRS AAQMISYEVS MGLIIVGVII STGSMNLSAI 190 200 210 220 230 240 VEAQRGDFGL LNWYWLPHLP MVALFFISAL AETNRPPFDL PEAESELVAG FMVEYSSTPY 250 260 270 280 290 300 LLFMAGEYIA VWLMCALTSV LFFGGWLSPI PGVPDGVLWM VAKMAAVFFV FAMVKAIVPR 310 320 330 340 YRYDQLMRIG WKVFLPLSLA WVVVVAFLAK FEVLGGFWAR WSIGA

References

[1]	"Identification of two genes of Rhodobacter capsulatus coding for proteins homologous to the ND1 and 23 kDa subunits of the mitochondrial Complex I." Dupuis A. FEBS Lett. 301:215-218(1992) [PubMed: 1568483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33303 / B10.
[2]	"Genetic disruption of the respiratory NADH-ubiquinone reductase of Rhodobacter capsulatus leads to an unexpected photosynthesis-negative phenotype." Dupuis A., Peinnequin A., Darrouzet E., Lunardi J. FEMS Microbiol. Lett. 148:107-114(1997) Cited for: DISRUPTION PHENOTYPE. Strain: ATCC 33303 / B10.
[3]	"Distal genes of the nuo operon of Rhodobacter capsulatus equivalent to the mitochondrial ND subunits are all essential for the biogenesis of the respiratory NADH-ubiquinone oxidoreductase." Dupuis A., Darrouzet E., Duborjal H., Pierrard B., Chevallet M., van Belzen R., Albracht S.P.J., Lunardi J. Mol. Microbiol. 28:531-541(1998) [PubMed: 9632256] [Abstract] Cited for: SUBCELLULAR LOCATION IN CHROMATOPHORE. Strain: ATCC 33303 / B10.
[4]	"Transmembrane orientation and topology of the NADH:quinone oxidoreductase putative quinone binding subunit NuoH." Roth R., Haegerhaell C. Biochim. Biophys. Acta 1504:352-362(2001) [PubMed: 11245799] [Abstract] Cited for: TOPOLOGY, SUBCELLULAR LOCATION. Strain: ATCC 11166 / DSM 1710 / IFO 16435 / LMG 2962 / NCIB 8254.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF029365 Genomic DNA. Translation: AAC24997.1.
PIR	S22368.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.6.99.5. 567.
Family and domain databases
HAMAP	MF_01350_B. NDH1_NuoH_B. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOH_RHOCA

Accession

Primary (citable) accession number: P42032

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)