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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

NDUFS8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi116Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi119Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi123Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi152Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi158Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
TYKY subunit
Gene namesi
Name:NDUFS8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 36Mitochondrion1 PublicationAdd BLAST36
ChainiPRO_000002001137 – 212NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialAdd BLAST176

Proteomic databases

PaxDbiP42028.
PeptideAtlasiP42028.
PRIDEiP42028.

Expressioni

Gene expression databases

BgeeiENSBTAG00000012072.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.2 Publications

Protein-protein interaction databases

IntActiP42028. 2 interactors.
STRINGi9913.ENSBTAP00000016013.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35I37-212[»]
5LDWelectron microscopy4.27I37-212[»]
5LDXelectron microscopy5.60I37-212[»]
ProteinModelPortaliP42028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 1334Fe-4S ferredoxin-type 1Add BLAST30
Domaini143 – 1724Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3256. Eukaryota.
COG1143. LUCA.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
HOVERGENiHBG006547.
InParanoidiP42028.
KOiK03941.
OMAiRGDLYYT.
OrthoDBiEOG091G0OQD.
TreeFamiTF105610.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCLTMPMLL RALAQAQAAR AGHASVRGLH SSAVAATYKY VNLREPSMDM
60 70 80 90 100
KSVTDRAAQT LLWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE
110 120 130 140 150
HALRRYPSGE ERCIACKLCE AVCPAQAITI EAEPRADGSR RTTRYDIDMT
160 170 180 190 200
KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE
210
IAANIQADYL YR
Length:212
Mass (Da):23,896
Last modified:November 1, 1995 - v1
Checksum:i4D5F95E4C918FEF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129T → I in AAI09907 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58717 mRNA. Translation: AAA30664.1.
BC109906 mRNA. Translation: AAI09907.1.
PIRiA38409.
RefSeqiNP_001289598.1. NM_001302669.1.
XP_005227041.1. XM_005226984.2.
UniGeneiBt.5483.

Genome annotation databases

EnsembliENSBTAT00000016013; ENSBTAP00000016013; ENSBTAG00000012072.
GeneIDi287027.
KEGGibta:287027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58717 mRNA. Translation: AAA30664.1.
BC109906 mRNA. Translation: AAI09907.1.
PIRiA38409.
RefSeqiNP_001289598.1. NM_001302669.1.
XP_005227041.1. XM_005226984.2.
UniGeneiBt.5483.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35I37-212[»]
5LDWelectron microscopy4.27I37-212[»]
5LDXelectron microscopy5.60I37-212[»]
ProteinModelPortaliP42028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP42028. 2 interactors.
STRINGi9913.ENSBTAP00000016013.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP42028.
PeptideAtlasiP42028.
PRIDEiP42028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000016013; ENSBTAP00000016013; ENSBTAG00000012072.
GeneIDi287027.
KEGGibta:287027.

Organism-specific databases

CTDi4728.

Phylogenomic databases

eggNOGiKOG3256. Eukaryota.
COG1143. LUCA.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
HOVERGENiHBG006547.
InParanoidiP42028.
KOiK03941.
OMAiRGDLYYT.
OrthoDBiEOG091G0OQD.
TreeFamiTF105610.

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Gene expression databases

BgeeiENSBTAG00000012072.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS8_BOVIN
AccessioniPrimary (citable) accession number: P42028
Secondary accession number(s): Q32KV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.