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P42028

- NDUS8_BOVIN

UniProt

P42028 - NDUS8_BOVIN

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

NDUFS8

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi119 – 1191Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi123 – 1231Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitochondrial respiratory chain complex I assembly Source: Ensembl
    2. response to oxidative stress Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    ReactomeiREACT_223064. Respiratory electron transport.

    Protein family/group databases

    TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-23kD
    Short name:
    CI-23kD
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    TYKY subunit
    Gene namesi
    Name:NDUFS8
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 29

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: Ensembl

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3636Mitochondrion1 PublicationAdd
    BLAST
    Chaini37 – 212176NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020011Add
    BLAST

    Proteomic databases

    PaxDbiP42028.
    PRIDEiP42028.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.

    Protein-protein interaction databases

    IntActiP42028. 2 interactions.
    STRINGi9913.ENSBTAP00000016013.

    Structurei

    3D structure databases

    ProteinModelPortaliP42028.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini104 – 133304Fe-4S ferredoxin-type 1Add
    BLAST
    Domaini143 – 172304Fe-4S ferredoxin-type 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 23 kDa subunit family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG1143.
    GeneTreeiENSGT00390000003049.
    HOGENOMiHOG000228289.
    HOVERGENiHBG006547.
    InParanoidiP42028.
    KOiK03941.
    OMAiTYKYVNL.
    OrthoDBiEOG7J9VQT.
    TreeFamiTF105610.

    Family and domain databases

    HAMAPiMF_01351. NDH1_NuoI.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view]
    PfamiPF12838. Fer4_7. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01971. NuoI. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42028-1 [UniParc]FASTAAdd to Basket

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    MRCLTMPMLL RALAQAQAAR AGHASVRGLH SSAVAATYKY VNLREPSMDM    50
    KSVTDRAAQT LLWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE 100
    HALRRYPSGE ERCIACKLCE AVCPAQAITI EAEPRADGSR RTTRYDIDMT 150
    KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE 200
    IAANIQADYL YR 212
    Length:212
    Mass (Da):23,896
    Last modified:November 1, 1995 - v1
    Checksum:i4D5F95E4C918FEF8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti129 – 1291T → I in AAI09907. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58717 mRNA. Translation: AAA30664.1.
    BC109906 mRNA. Translation: AAI09907.1.
    PIRiA38409.
    RefSeqiXP_005227040.1. XM_005226983.1.
    XP_005227041.1. XM_005226984.1.
    UniGeneiBt.5483.

    Genome annotation databases

    EnsembliENSBTAT00000016013; ENSBTAP00000016013; ENSBTAG00000012072.
    GeneIDi287027.
    KEGGibta:287027.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58717 mRNA. Translation: AAA30664.1 .
    BC109906 mRNA. Translation: AAI09907.1 .
    PIRi A38409.
    RefSeqi XP_005227040.1. XM_005226983.1.
    XP_005227041.1. XM_005226984.1.
    UniGenei Bt.5483.

    3D structure databases

    ProteinModelPortali P42028.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P42028. 2 interactions.
    STRINGi 9913.ENSBTAP00000016013.

    Protein family/group databases

    TCDBi 3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PaxDbi P42028.
    PRIDEi P42028.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000016013 ; ENSBTAP00000016013 ; ENSBTAG00000012072 .
    GeneIDi 287027.
    KEGGi bta:287027.

    Organism-specific databases

    CTDi 4728.

    Phylogenomic databases

    eggNOGi COG1143.
    GeneTreei ENSGT00390000003049.
    HOGENOMi HOG000228289.
    HOVERGENi HBG006547.
    InParanoidi P42028.
    KOi K03941.
    OMAi TYKYVNL.
    OrthoDBi EOG7J9VQT.
    TreeFami TF105610.

    Enzyme and pathway databases

    Reactomei REACT_223064. Respiratory electron transport.

    Miscellaneous databases

    NextBioi 20806549.

    Family and domain databases

    HAMAPi MF_01351. NDH1_NuoI.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view ]
    Pfami PF12838. Fer4_7. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01971. NuoI. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex I is encoded in chloroplast genomes."
      Dupuis A., Skehel J.M., Walker J.E.
      Biochemistry 30:2954-2960(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-59.
      Tissue: Heart.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiNDUS8_BOVIN
    AccessioniPrimary (citable) accession number: P42028
    Secondary accession number(s): Q32KV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3