Reviewed, UniProtKB/Swiss-Prot P42028 (NDUS8_BOVIN)

Names and origin

Protein names

Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Complex I-23kD
      Short name=CI-23kD
    TYKY subunit

Gene names

Name:

NDUFS8

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from direct assay. Source: UniProtKB transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Mitochondrion Ref.1
	Chain	37 – 212	176	NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial		PRO_0000020011
Regions
	Domain	104 – 133	30	4Fe-4S ferredoxin-type 1
	Domain	143 – 172	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	113	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	116	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	119	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	123	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	152	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	155	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	158	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	162	1	Iron-sulfur 1 (4Fe-4S) By similarity
Experimental info
	Sequence conflict	129	1	T → I in AAI09907. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P42028-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4D5F95E4C918FEF8	FASTA	212	23,896
	10 20 30 40 50 60 MRCLTMPMLL RALAQAQAAR AGHASVRGLH SSAVAATYKY VNLREPSMDM KSVTDRAAQT 70 80 90 100 110 120 LLWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE HALRRYPSGE ERCIACKLCE 130 140 150 160 170 180 AVCPAQAITI EAEPRADGSR RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFSTETH 190 200 210 EELLYNKEKL LNNGDKWEAE IAANIQADYL YR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex I is encoded in chloroplast genomes." Dupuis A., Skehel J.M., Walker J.E. Biochemistry 30:2954-2960(1991) [PubMed: 1901022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-59. Tissue: Heart.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M58717 mRNA. Translation: AAA30664.1. BC109906 mRNA. Translation: AAI09907.1.
IPI	IPI00707212.
PIR	A38409.
RefSeq	NP_777243.2.
UniGene	Bt.5483
3D structure databases
HSSP	HSSP built from PDB template 2FDN based on UniProtKB P00198.
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.6.1. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
Ensembl	ENSBTAG00000012072. Bos taurus. [Contig view]
GeneID	287027.
KEGG	bta:287027.
Phylogenomic databases
HOVERGEN	P42028.
OMA	P42028. QADFLYR.
Enzyme and pathway databases
BRENDA	1.6.5.3. 251. 1.6.99.3. 251.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS8_BOVIN

Accession

Primary (citable) accession number: P42028
Secondary accession number(s): Q32KV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)