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Reviewed, UniProtKB/Swiss-Prot P42028 (NDUS8_BOVIN)

Last modified November 25, 2008. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Complex I-23kD
      Short name=CI-23kD
    TYKY subunit
Gene names
Name: NDUFS8
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activity

NADH + ubiquinone = NAD(+) + ubiquinol.

NADH + acceptor = NAD(+) + reduced acceptor.

Cofactor

Binds 2 4Fe-4S clusters per subunit By similarity.

Subunit structure

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.

Subcellular location

Mitochondrion.

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3636Mitochondrion
Chain37 – 212176NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
PRO_0000020011

Regions

Domain104 – 133304Fe-4S ferredoxin-type 1
Domain143 – 172304Fe-4S ferredoxin-type 2

Sites

Metal binding1131Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1161Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1191Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1231Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1521Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1551Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1581Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1621Iron-sulfur 1 (4Fe-4S) By similarity

Experimental info

Sequence conflict1291T → I in AAI09907. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P42028-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4D5F95E4C918FEF8

FASTA21223,896
        10         20         30         40         50         60 
MRCLTMPMLL RALAQAQAAR AGHASVRGLH SSAVAATYKY VNLREPSMDM KSVTDRAAQT 

        70         80         90        100        110        120 
LLWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE HALRRYPSGE ERCIACKLCE 

       130        140        150        160        170        180 
AVCPAQAITI EAEPRADGSR RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFSTETH 

       190        200        210 
EELLYNKEKL LNNGDKWEAE IAANIQADYL YR 

« Hide

References

« Hide 'large scale' references
[1]"A homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex I is encoded in chloroplast genomes."
Dupuis A., Skehel J.M., Walker J.E.
Biochemistry 30:2954-2960(1991) [PubMed: 1901022] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-59.
Tissue: Heart.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

M58717 mRNA. Translation: AAA30664.1.
BC109906 mRNA. Translation: AAI09907.1.
PIRA38409.
RefSeqNP_777243.2.
UniGeneBt.5483

3D structure databases

HSSPHSSP built from PDB template 2FDN based on UniProtKB P00198.
ModBaseSearch...

Genome annotation databases

EnsemblENSBTAG00000012072. Bos taurus. [Contig view]
GeneID287027.
KEGGbta:287027.

Phylogenomic databases

HOVERGENP42028.

Family and domain databases

InterProIPR001450. 4Fe4S_Fe_S_bd.
IPR010226. NADH_quinone_OxRdtase_I.
[Graphical view]
PfamPF00037. Fer4. 2 hits.
[Graphical view]
PRINTSPR00353. 4FE4SFRDOXIN.
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS8_BOVIN
AccessionPrimary (citable) accession number: P42028
Secondary accession number(s): Q32KV7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 25, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents