NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial precursor

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P42028 (NDUS8_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-23kD
Short name=CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
TYKY subunit

Gene names

Name:

NDUFS8

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Repeat Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex I assembly Inferred from electronic annotation. Source: Compara response to oxidative stress Inferred from direct assay PubMed 12857734. Source: UniProtKB
Cellular_component	mitochondrial respiratory chain complex I Inferred from electronic annotation. Source: Compara
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 36

Mitochondrion Ref.1

Chain

37 – 212

176

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

PRO_0000020011

Regions

Domain

104 – 133

4Fe-4S ferredoxin-type 1

Domain

143 – 172

4Fe-4S ferredoxin-type 2

Sites

Metal binding

113

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

116

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

119

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

123

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

152

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

155

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

158

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

162

Iron-sulfur 1 (4Fe-4S) By similarity

Experimental info

Sequence conflict

129

T → I in AAI09907. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P42028 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4D5F95E4C918FEF8
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FASTA

212

23,896

        10         20         30         40         50         60 
MRCLTMPMLL RALAQAQAAR AGHASVRGLH SSAVAATYKY VNLREPSMDM KSVTDRAAQT 

        70         80         90        100        110        120 
LLWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE HALRRYPSGE ERCIACKLCE 

       130        140        150        160        170        180 
AVCPAQAITI EAEPRADGSR RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFSTETH 

       190        200        210 
EELLYNKEKL LNNGDKWEAE IAANIQADYL YR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex I is encoded in chloroplast genomes." Dupuis A., Skehel J.M., Walker J.E. Biochemistry 30:2954-2960(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-59. Tissue: Heart.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58717 mRNA. Translation: AAA30664.1. BC109906 mRNA. Translation: AAI09907.1.
IPI	IPI00707212.
PIR	A38409.
RefSeq	NP_777243.2. NM_174818.3.
UniGene	Bt.5483.
3D structure databases
ProteinModelPortal	P42028.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42028. 2 interactions.
STRING	9913.ENSBTAP00000016013.
Protein family/group databases
TCDB	3.D.1.6.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PaxDb	P42028.
PRIDE	P42028.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000016013; ENSBTAP00000016013; ENSBTAG00000012072.
GeneID	287027.
KEGG	bta:287027.
Organism-specific databases
CTD	4728.
Phylogenomic databases
eggNOG	COG1143.
GeneTree	ENSGT00390000003049.
HOGENOM	HOG000228289.
HOVERGEN	HBG006547.
InParanoid	P42028.
KO	K03941.
OMA	CPAMAIT.
OrthoDB	EOG43R3NR.
Family and domain databases
Gene3D	1.10.1060.10. 1 hit.
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012285. Fum_reductase_C. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF12838. Fer4_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806549.

Entry information

Entry name

NDUS8_BOVIN

Accession

Primary (citable) accession number: P42028
Secondary accession number(s): Q32KV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents