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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

NDUFS8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi119 – 1191Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi123 – 1231Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiREACT_281214. Respiratory electron transport.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
TYKY subunit
Gene namesi
Name:NDUFS8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 29

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636Mitochondrion1 PublicationAdd
BLAST
Chaini37 – 212176NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000020011Add
BLAST

Proteomic databases

PaxDbiP42028.
PRIDEiP42028.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

IntActiP42028. 2 interactions.
STRINGi9913.ENSBTAP00000016013.

Structurei

3D structure databases

ProteinModelPortaliP42028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 133304Fe-4S ferredoxin-type 1Add
BLAST
Domaini143 – 172304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG1143.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
HOVERGENiHBG006547.
InParanoidiP42028.
KOiK03941.
OMAiDLFKCIY.
OrthoDBiEOG7J9VQT.
TreeFamiTF105610.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCLTMPMLL RALAQAQAAR AGHASVRGLH SSAVAATYKY VNLREPSMDM
60 70 80 90 100
KSVTDRAAQT LLWTELIRGL GMTLSYLFRE PATINYPFEK GPLSPRFRGE
110 120 130 140 150
HALRRYPSGE ERCIACKLCE AVCPAQAITI EAEPRADGSR RTTRYDIDMT
160 170 180 190 200
KCIYCGFCQE ACPVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE
210
IAANIQADYL YR
Length:212
Mass (Da):23,896
Last modified:November 1, 1995 - v1
Checksum:i4D5F95E4C918FEF8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291T → I in AAI09907 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58717 mRNA. Translation: AAA30664.1.
BC109906 mRNA. Translation: AAI09907.1.
PIRiA38409.
RefSeqiNP_001289598.1. NM_001302669.1.
XP_005227041.1. XM_005226984.2.
UniGeneiBt.5483.

Genome annotation databases

EnsembliENSBTAT00000016013; ENSBTAP00000016013; ENSBTAG00000012072.
GeneIDi287027.
KEGGibta:287027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58717 mRNA. Translation: AAA30664.1.
BC109906 mRNA. Translation: AAI09907.1.
PIRiA38409.
RefSeqiNP_001289598.1. NM_001302669.1.
XP_005227041.1. XM_005226984.2.
UniGeneiBt.5483.

3D structure databases

ProteinModelPortaliP42028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP42028. 2 interactions.
STRINGi9913.ENSBTAP00000016013.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP42028.
PRIDEiP42028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000016013; ENSBTAP00000016013; ENSBTAG00000012072.
GeneIDi287027.
KEGGibta:287027.

Organism-specific databases

CTDi4728.

Phylogenomic databases

eggNOGiCOG1143.
GeneTreeiENSGT00390000003049.
HOGENOMiHOG000228289.
HOVERGENiHBG006547.
InParanoidiP42028.
KOiK03941.
OMAiDLFKCIY.
OrthoDBiEOG7J9VQT.
TreeFamiTF105610.

Enzyme and pathway databases

ReactomeiREACT_281214. Respiratory electron transport.

Miscellaneous databases

NextBioi20806549.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex I is encoded in chloroplast genomes."
    Dupuis A., Skehel J.M., Walker J.E.
    Biochemistry 30:2954-2960(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 37-59.
    Tissue: Heart.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiNDUS8_BOVIN
AccessioniPrimary (citable) accession number: P42028
Secondary accession number(s): Q32KV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 1, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.