P42027 (NDUS7_BRAOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial EC=1.6.5.3 EC=1.6.99.3
Organism	Brassica oleracea (Wild cabbage)
Taxonomic identifier	3712 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Subunit structure	Complex I is composed of about 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 215		NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial		PRO_0000020034
Sites
	Metal binding	90	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	91	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	155	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	185	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P42027 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E39E62D038C11FC3	FASTA	215	23,839
	10 20 30 40 50 60 MAMITRNTAT RLPLVLQSHR AAAVSHLHTS LPALSPATTP TSYTRPGPPS TSAPPPGLSK 70 80 90 100 110 120 TAEFVISKVD DLMNWARRGS IWPMTFGLAC CAVEMMHTGA ARYDLDRFGI IFRPSPRQSD 130 140 150 160 170 180 CMIVAGTLTN KMAPALRKVY DQMPEPRWVI SMGSCANGGG YYHYSYSVVR GCDRIVPVDI 190 200 210 YVPGCPPTAE ALLYGLLQLQ KKINRRKDFL HWWNK

References

[1]

"Nucleotide sequence of a cDNA clone from broccoli with high identity with the PSST subunit of NADH:ubiquinone oxidoreductase." Pogson B.J., Downs C.G., Davies K.M., Morris S.C., Buchanan-Wollaston V. Plant Physiol. 108:859-860(1995) [PubMed: 7610188] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Shogun. Tissue: Floret.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82274 mRNA. Translation: CAA57725.1.
PIR	S48826.
3D structure databases
ProteinModelPortal	P42027.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UQ_OxRdtase_20Kd_su. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. NuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS7_BRAOL

Accession

Primary (citable) accession number: P42027

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 21, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program