NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor - Brassica oleracea (Wild cabbage)

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Reviewed, UniProtKB/Swiss-Prot P42027 (NDUS7_BRAOL)

Last modified November 25, 2008. Version 52. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names	Recommended name: NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial EC=1.6.5.3 EC=1.6.99.3 Alternative name(s): Complex I-20kD Short name=CI-20kD
Organism	Brassica oleracea (Wild cabbage)
Taxonomic identifier	3712 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion

Chain

? – 215

NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial

PRO_0000020034

Sites

Metal binding

1

Iron-sulfur (4Fe-4S) Potential

Metal binding

1

Iron-sulfur (4Fe-4S) Potential

Metal binding

1

Iron-sulfur (4Fe-4S) Potential

Metal binding

1

Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P42027-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: E39E62D038C11FC3
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215

23,839

        10         20         30         40         50         60 
MAMITRNTAT RLPLVLQSHR AAAVSHLHTS LPALSPATTP TSYTRPGPPS TSAPPPGLSK 

        70         80         90        100        110        120 
TAEFVISKVD DLMNWARRGS IWPMTFGLAC CAVEMMHTGA ARYDLDRFGI IFRPSPRQSD 

       130        140        150        160        170        180 
CMIVAGTLTN KMAPALRKVY DQMPEPRWVI SMGSCANGGG YYHYSYSVVR GCDRIVPVDI 

       190        200        210 
YVPGCPPTAE ALLYGLLQLQ KKINRRKDFL HWWNK

References

[1]

"Nucleotide sequence of a cDNA clone from broccoli with high identity with the PSST subunit of NADH:ubiquinone oxidoreductase."
Pogson B.J., Downs C.G., Davies K.M., Morris S.C., Buchanan-Wollaston V.
Plant Physiol. 108:859-860(1995) [PubMed: 7610188] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Shogun.
Tissue: Floret.

Cross-references

Sequence databases
	X82274 mRNA. Translation: CAA57725.1.
PIR	S48826.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR006138. NADH_DHase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS7_BRAOL

Accession

Primary (citable) accession number: P42027

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents