P42026 (NDUS7_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-20kD
Short name=CI-20kD
NADH-ubiquinone oxidoreductase 20 kDa subunit
PSST subunit

Gene names

Name:

NDUFS7

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Subunit structure	Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex I assembly Inferred from sequence or structural similarity. Source: UniProtKB transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain complex I Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Mitochondrion
	Chain	38 – 216	179	NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial		PRO_0000020026
Sites
	Metal binding	91	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	92	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	156	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	186	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P42026 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 514B8A63C59BE641	FASTA	216	23,771
	10 20 30 40 50 60 MAALAALRLL HPILAVRSGV GAALQVRGVH SSMAADSPSS TQPAVSQARA VVPKPAALPS 70 80 90 100 110 120 SRGEYVVAKL DDLINWARRS SLWPMTFGLA CCAVEMMHMA APRYDMDRFG VVFRASPRQS 130 140 150 160 170 180 DVMIVAGTLT NKMAPALRKV YDQMPEPRYV VSMGSCANGG GYYHYSYSVV RGCDRIVPVD 190 200 210 IYVPGCPPTA EALLYGILQL QKKIKREKRL RIWYRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NADH: ubiquinone oxidoreductase from bovine heart mitochondria. A fourth nuclear encoded subunit with a homologue encoded in chloroplast genomes." Arizmendi J.M., Runswick M.J., Skehel J.M., Walker J.E. FEBS Lett. 301:237-242(1992) [PubMed: 1577158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Heart.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65020 mRNA. Translation: CAA46154.1. BC109716 mRNA. Translation: AAI09717.1.
IPI	IPI00697645.
PIR	S22371.
RefSeq	NP_001033111.1. NM_001038022.1.
UniGene	Bt.315.
3D structure databases
ProteinModelPortal	P42026.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42026. 2 interactions.
STRING	P42026.
Protein family/group databases
TCDB	3.D.1.6.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PRIDE	P42026.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000025870; ENSBTAP00000025870; ENSBTAG00000019419.
GeneID	338079.
KEGG	bta:338079.
Organism-specific databases
CTD	374291.
Phylogenomic databases
eggNOG	maNOG17775.
HOVERGEN	HBG001576.
InParanoid	P42026.
OMA	MKMAERV.
PhylomeDB	P42026.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UQ_OxRdtase_20Kd_su. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
KO	K03940.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. NuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS7_BOVIN

Accession

Primary (citable) accession number: P42026
Secondary accession number(s): Q2TBS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 16, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program