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P41996

- CPG2_CAEEL

UniProt

P41996 - CPG2_CAEEL

Protein

Chondroitin proteoglycan-2

Gene

cpg-2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (15 Aug 2003)
      Previous versions | rss
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    Functioni

    Required for polar body extrusion during cytokinesis in embryo development. Affects cortical granule size. Has roles in meiotic chromosome segregation, osmotic barrier function and polarization in conjunction with cpg-2. Binds chitin.4 Publications

    GO - Molecular functioni

    1. chitin binding Source: UniProtKB

    GO - Biological processi

    1. chitin metabolic process Source: InterPro
    2. eggshell formation Source: UniProtKB
    3. embryo development Source: UniProtKB
    4. mitotic cytokinesis Source: UniProtKB
    5. regulation of cytokinesis Source: WormBase

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    Chitin-binding

    Protein family/group databases

    CAZyiCBM14. Carbohydrate-Binding Module Family 14.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chondroitin proteoglycan-2
    Alternative name(s):
    Cytokinesis protein B0280.5
    Gene namesi
    Name:cpg-2
    ORF Names:B0280.5
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiB0280.5; CE31868; WBGene00015102; cpg-2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    Pathology & Biotechi

    Disruption phenotypei

    Worms lacking cpg-2 and cpg-1 exhibit defects in cytokinesis during embryo development more specifically meiotic chromosome segregation, polar-body extrusion, osmotic barrier function and polarization. Embryos lacking cpg-2 and cpg-1 proteins have multiple nuclei lacking plasma membranes and may also have weak egg shells. Oocytes lacking cpg-2 and cpg-1 show cortical granules that are reduced in size.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 524506Chondroitin proteoglycan-2PRO_0000023617Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi54 ↔ 67PROSITE-ProRule annotation
    Glycosylationi103 – 1031O-linked (Xyl...) (chondroitin sulfate)1 Publication
    Glycosylationi119 – 1191O-linked (Xyl...) (chondroitin sulfate)1 Publication
    Disulfide bondi168 ↔ 181PROSITE-ProRule annotation
    Glycosylationi208 – 2081O-linked (Xyl...) (chondroitin sulfate)1 Publication
    Glycosylationi212 – 2121O-linked (Xyl...) (chondroitin sulfate)1 Publication
    Disulfide bondi277 ↔ 290PROSITE-ProRule annotation
    Disulfide bondi337 ↔ 350PROSITE-ProRule annotation
    Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi432 ↔ 445PROSITE-ProRule annotation
    Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi500 ↔ 514PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Proteoglycan

    Proteomic databases

    PaxDbiP41996.

    Expressioni

    Tissue specificityi

    Expressed in the germline.1 Publication

    Developmental stagei

    Expressed throughout development but appears to be up-regulated in adults.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi41203. 1 interaction.
    MINTiMINT-1061878.
    STRINGi6239.B0280.5.

    Structurei

    3D structure databases

    ProteinModelPortaliP41996.
    SMRiP41996. Positions 27-80, 138-192, 251-295, 309-358, 415-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 7858Chitin-binding type-2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini135 – 19258Chitin-binding type-2 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini244 – 30158Chitin-binding type-2 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini306 – 36156Chitin-binding type-2 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini400 – 45657Chitin-binding type-2 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini469 – 52456Chitin-binding type-2 6PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi75 – 258184Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 6 chitin-binding type-2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG241358.
    GeneTreeiENSGT00650000093766.
    HOGENOMiHOG000017714.
    InParanoidiP41996.
    OMAiFDCSTDG.
    PhylomeDBiP41996.

    Family and domain databases

    Gene3Di2.170.140.10. 5 hits.
    InterProiIPR002557. Chitin-bd_dom.
    [Graphical view]
    PfamiPF01607. CBM_14. 6 hits.
    [Graphical view]
    SMARTiSM00494. ChtBD2. 6 hits.
    [Graphical view]
    SUPFAMiSSF57625. SSF57625. 6 hits.
    PROSITEiPS50940. CHIT_BIND_II. 6 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P41996-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTVAALTLL AFATAANGQF LQDCTNALDG LYALGECEPQ FLTCSGGIAR    50
    IMDCPADLIY NEPLLICDWR HNVIGCEGSG ESSGETSGEG SGESSGEASG 100
    EGSGEASGEG SGEASGEGSG EASGEGSGSG EETVENVCEN LEDGAYSSGG 150
    CTTYYFFCTT NTARFLSCPT PLFYDADSQK CIWKSLVEEC KEDLTITDGS 200
    GETSGEGSGE ASGEASGEGS GEASGESSGQ GSGEASGEGS GELEPTCEGK 250
    ADGIHPNGVC STNFLTCSGG IARIMDCPAS LVFNPTILVC DWPRDVAECA 300
    GLPTPQPTCE EDGYFSFGQC SSSFTACTNG RAIVMFCPAG LKFSESTVRC 350
    DYESNVSECQ ETSGEESGEA SGEQSGEGSG EASGEASGES SGEGSGVEEQ 400
    NQCVGLDNGL HAIGCSPRVL SCQNGHVDIF ECPSSLVFND QSLICDYPQT 450
    SLKCLIEDTI LIDETPIAAF DCSTDGLFSD GLCSATYHQC TAGQLINFTC 500
    AASNAVFSAA NTECVDSSTL LQCH 524
    Length:524
    Mass (Da):53,652
    Last modified:August 15, 2003 - v3
    Checksum:iFFCB9A750385FB34
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ340624 mRNA. Translation: ABC65812.1.
    FO080148 Genomic DNA. Translation: CCD61602.1.
    PIRiT15299.
    RefSeqiNP_498551.3. NM_066150.10.
    UniGeneiCel.17405.

    Genome annotation databases

    EnsemblMetazoaiB0280.5; B0280.5; WBGene00015102.
    GeneIDi175991.
    KEGGicel:CELE_B0280.5.
    UCSCiB0280.5. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ340624 mRNA. Translation: ABC65812.1 .
    FO080148 Genomic DNA. Translation: CCD61602.1 .
    PIRi T15299.
    RefSeqi NP_498551.3. NM_066150.10.
    UniGenei Cel.17405.

    3D structure databases

    ProteinModelPortali P41996.
    SMRi P41996. Positions 27-80, 138-192, 251-295, 309-358, 415-451.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 41203. 1 interaction.
    MINTi MINT-1061878.
    STRINGi 6239.B0280.5.

    Protein family/group databases

    CAZyi CBM14. Carbohydrate-Binding Module Family 14.

    Proteomic databases

    PaxDbi P41996.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai B0280.5 ; B0280.5 ; WBGene00015102 .
    GeneIDi 175991.
    KEGGi cel:CELE_B0280.5.
    UCSCi B0280.5. c. elegans.

    Organism-specific databases

    CTDi 175991.
    WormBasei B0280.5 ; CE31868 ; WBGene00015102 ; cpg-2.

    Phylogenomic databases

    eggNOGi NOG241358.
    GeneTreei ENSGT00650000093766.
    HOGENOMi HOG000017714.
    InParanoidi P41996.
    OMAi FDCSTDG.
    PhylomeDBi P41996.

    Miscellaneous databases

    NextBioi 890638.

    Family and domain databases

    Gene3Di 2.170.140.10. 5 hits.
    InterProi IPR002557. Chitin-bd_dom.
    [Graphical view ]
    Pfami PF01607. CBM_14. 6 hits.
    [Graphical view ]
    SMARTi SM00494. ChtBD2. 6 hits.
    [Graphical view ]
    SUPFAMi SSF57625. SSF57625. 6 hits.
    PROSITEi PS50940. CHIT_BIND_II. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of novel chondroitin proteoglycans in Caenorhabditis elegans: embryonic cell division depends on CPG-1 and CPG-2."
      Olson S.K., Bishop J.R., Yates J.R., Oegema K., Esko J.D.
      J. Cell Biol. 173:985-994(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, GLYCOSYLATION AT SER-103; SER-119; SER-208 AND SER-212, FUNCTION, DISRUPTION PHENOTYPE.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    4. "Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C. elegans germ cell development."
      Lee M.-H., Schedl T.
      Genes Dev. 15:2408-2420(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegans embryo."
      Johnston W.L., Krizus A., Dennis J.W.
      BMC Biol. 4:35-35(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase."
      Bembenek J.N., Richie C.T., Squirrell J.M., Campbell J.M., Eliceiri K.W., Poteryaev D., Spang A., Golden A., White J.G.
      Development 134:3837-3848(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCPG2_CAEEL
    AccessioniPrimary (citable) accession number: P41996
    Secondary accession number(s): Q1A3T5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: August 15, 2003
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3