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Protein

Muscarinic acetylcholine receptor M3

Gene

CHRM3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-BTA-390648. Muscarinic acetylcholine receptors.
R-BTA-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M3
Gene namesi
Name:CHRM3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 28

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 67ExtracellularBy similarityAdd BLAST67
Transmembranei68 – 91Helical; Name=1By similarityAdd BLAST24
Topological domaini92 – 104CytoplasmicBy similarityAdd BLAST13
Transmembranei105 – 125Helical; Name=2By similarityAdd BLAST21
Topological domaini126 – 142ExtracellularBy similarityAdd BLAST17
Transmembranei143 – 164Helical; Name=3By similarityAdd BLAST22
Topological domaini165 – 184CytoplasmicBy similarityAdd BLAST20
Transmembranei185 – 207Helical; Name=4By similarityAdd BLAST23
Topological domaini208 – 229ExtracellularBy similarityAdd BLAST22
Transmembranei230 – 252Helical; Name=5By similarityAdd BLAST23
Topological domaini253 – 492CytoplasmicBy similarityAdd BLAST240
Transmembranei493 – 513Helical; Name=6By similarityAdd BLAST21
Topological domaini514 – 527ExtracellularBy similarityAdd BLAST14
Transmembranei528 – 547Helical; Name=7By similarityAdd BLAST20
Topological domaini548 – 590CytoplasmicBy similarityAdd BLAST43

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690271 – 590Muscarinic acetylcholine receptor M3Add BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi6N-linked (GlcNAc...)Sequence analysis1
Glycosylationi7N-linked (GlcNAc...)Sequence analysis1
Glycosylationi15N-linked (GlcNAc...)Sequence analysis1
Glycosylationi41N-linked (GlcNAc...)Sequence analysis1
Glycosylationi48N-linked (GlcNAc...)Sequence analysis1
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi141 ↔ 221PROSITE-ProRule annotation
Modified residuei385PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP41984.
PRIDEiP41984.

PTM databases

iPTMnetiP41984.

Expressioni

Gene expression databases

BgeeiENSBTAG00000008059.

Interactioni

Subunit structurei

Homodimer; the dimers can form tetramers. Interacts with NALCN.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010601.

Structurei

3D structure databases

ProteinModelPortaliP41984.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi275 – 281Basolateral sorting signalBy similarity7

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP41984.
KOiK04131.
OMAiKSMDDGG.
OrthoDBiEOG091G06VI.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41984-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLHNNNTTS PLFPNISSSW IHGPSDTGLP PGTVTHFGSY NISRAAGNLS
60 70 80 90 100
SPNGTTSDPL GGHTIWQVVF IAFLTGVLAL VTIIGNILVI VAFKVNKQLK
110 120 130 140 150
TVNNYFLLSL ACADLIIGVI SMNLFTTYII MNRWALGNLA CDLWLSIDYV
160 170 180 190 200
ASNASVMNLL VISFDRYFSI TRPLTYRAKR TTKRAGVMIG LAWVISFILW
210 220 230 240 250
APAILFWQYF VGKRTVPPGE CFIQFLSEPT ITFGTAIAAF YMPVTIMTIL
260 270 280 290 300
YWRIYKETEK RTKELAGLQA SGTEAEAENF VHPTGSSRSC SSYELQQQSM
310 320 330 340 350
KRSARRKYGR CHFWFTTKSW KPSAEQMDQD HSSSDSWNNN DAAASLENSA
360 370 380 390 400
SSDEEDIGSE TRAIYSIVLK LPGHSTILNS TKLPSSDNLQ VPEEELGSVG
410 420 430 440 450
LERKPSKLQT QQSMDDGGSF QKSFSKLPIQ LESAVDTAKA SDVNSSVGKT
460 470 480 490 500
TATLPLSFKE ATLAKRFALK TRSQITKRKR MSLIKEKKAA QTLSAILLAF
510 520 530 540 550
IITWTPYNIM VLVNTFCDSC IPKTYWNLGY WLCYINSTVN PVCYALCNKT
560 570 580 590
FRNTFKMLLL CQCDKRKRRK QQYQQRQSVI FHKRVPEQAL
Length:590
Mass (Da):66,103
Last modified:November 1, 1995 - v1
Checksum:i4DE04EDE33CCA8D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti424F → S in AAA30653 (Ref. 2) Curated1
Sequence conflicti438A → G (Ref. 2) Curated1
Sequence conflicti440A → G (Ref. 2) Curated1
Sequence conflicti452A → G in AAA30653 (Ref. 2) Curated1
Sequence conflicti461A → G in AAA30653 (Ref. 2) Curated1
Sequence conflicti467F → L in AAA30653 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08286 mRNA. Translation: AAA51866.1.
L27103 mRNA. Translation: AAA30653.1.
PIRiS47572.
RefSeqiNP_776695.1. NM_174270.2.
UniGeneiBt.501.

Genome annotation databases

EnsembliENSBTAT00000010601; ENSBTAP00000010601; ENSBTAG00000008059.
GeneIDi281685.
KEGGibta:281685.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08286 mRNA. Translation: AAA51866.1.
L27103 mRNA. Translation: AAA30653.1.
PIRiS47572.
RefSeqiNP_776695.1. NM_174270.2.
UniGeneiBt.501.

3D structure databases

ProteinModelPortaliP41984.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010601.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP41984.

Proteomic databases

PaxDbiP41984.
PRIDEiP41984.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010601; ENSBTAP00000010601; ENSBTAG00000008059.
GeneIDi281685.
KEGGibta:281685.

Organism-specific databases

CTDi1131.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP41984.
KOiK04131.
OMAiKSMDDGG.
OrthoDBiEOG091G06VI.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-BTA-390648. Muscarinic acetylcholine receptors.
R-BTA-416476. G alpha (q) signalling events.

Gene expression databases

BgeeiENSBTAG00000008059.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM3_BOVIN
AccessioniPrimary (citable) accession number: P41984
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 5, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.