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P41972

- SYI1_STAAU

UniProt

P41972 - SYI1_STAAU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.By similarity

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Cofactori

    Binds 1 zinc ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylate
    Binding sitei598 – 5981ATPBy similarity
    Binding sitei632 – 6321tRNA
    Binding sitei640 – 6401tRNA
    Metal bindingi886 – 8861Zinc
    Metal bindingi889 – 8891Zinc
    Metal bindingi906 – 9061Zinc
    Metal bindingi909 – 9091Zinc

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:ileS
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 917917Isoleucine--tRNA ligasePRO_0000098463Add
    BLAST

    Proteomic databases

    PRIDEiP41972.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1
    917
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 63
    Helixi20 – 3314
    Helixi36 – 438
    Turni44 – 463
    Beta strandi58 – 614
    Helixi65 – 8218
    Turni83 – 853
    Beta strandi93 – 953
    Helixi99 – 10810
    Helixi117 – 14024
    Turni141 – 1433
    Helixi157 – 17216
    Beta strandi176 – 18611
    Turni187 – 1904
    Helixi195 – 1973
    Beta strandi198 – 20912
    Beta strandi212 – 2143
    Helixi220 – 2223
    Beta strandi230 – 2323
    Helixi234 – 2363
    Helixi237 – 2393
    Beta strandi242 – 2454
    Turni256 – 2594
    Turni265 – 2684
    Helixi269 – 2724
    Turni273 – 2764
    Turni290 – 2923
    Helixi301 – 3033
    Beta strandi306 – 3127
    Beta strandi318 – 3203
    Helixi333 – 3364
    Turni337 – 3393
    Beta strandi340 – 3423
    Beta strandi351 – 3533
    Beta strandi363 – 3653
    Helixi369 – 3724
    Helixi375 – 3773
    Helixi380 – 3834
    Beta strandi388 – 39710
    Turni398 – 4003
    Beta strandi405 – 41410
    Helixi416 – 42813
    Beta strandi430 – 4345
    Helixi435 – 44713
    Beta strandi454 – 4563
    Beta strandi458 – 4603
    Beta strandi469 – 4713
    Helixi477 – 49014
    Helixi493 – 4975
    Helixi500 – 5034
    Beta strandi504 – 5074
    Beta strandi511 – 5133
    Helixi527 – 5326
    Helixi534 – 5374
    Turni538 – 5414
    Beta strandi547 – 5559
    Helixi556 – 5594
    Helixi562 – 57413
    Beta strandi578 – 5858
    Beta strandi591 – 5944
    Beta strandi598 – 6014
    Helixi606 – 6127
    Helixi615 – 6239
    Helixi635 – 65723
    Helixi664 – 6674
    Helixi671 – 6733
    Helixi676 – 69722
    Helixi701 – 71313
    Helixi714 – 7196
    Helixi720 – 7289
    Helixi736 – 75621
    Turni757 – 7593
    Helixi761 – 7688
    Beta strandi775 – 7784
    Helixi779 – 7813
    Helixi792 – 81726
    Helixi824 – 8263
    Beta strandi827 – 8326
    Helixi839 – 8424
    Helixi843 – 8453
    Helixi849 – 8524
    Beta strandi855 – 8606
    Beta strandi868 – 8714
    Beta strandi874 – 8807
    Beta strandi882 – 8854
    Turni887 – 8893
    Beta strandi892 – 8943
    Beta strandi897 – 8993
    Beta strandi902 – 9054
    Helixi907 – 9148

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FFYX-ray2.20A1-917[»]
    1QU2X-ray2.20A1-917[»]
    1QU3X-ray2.90A1-917[»]
    ProteinModelPortaliP41972.
    SMRiP41972. Positions 1-917.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP41972.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi595 – 5995"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.By similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41972-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETLLMP KTDFPMRGGL PNKEPQIQEK WDAEDQYHKA LEKNKGNETF    50
    ILHDGPPYAN GNLHMGHALN KILKDFIVRY KTMQGFYAPY VPGWDTHGLP 100
    IEQALTKKGV DRKKMSTAEF REKCKEFALE QIELQKKDFR RLGVRGDFND 150
    PYITLKPEYE AAQIRIFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY 200
    HDKRSASIYV AFNVKDDKGV VDADAKFIIW TTTPWTIPSN VAITVHPELK 250
    YGQYNVNGEK YIIAEALSDA VAEALDWDKA SIKLEKEYTG KELEYVVAQH 300
    PFLDRESLVI NGDHVTTDAG TGCVHTAPGH GEDDYIVGQK YELPVISPID 350
    DKGVFTEEGG QFEGMFYDKA NKAVTDLLTE KGALLKLDFI THSYPHDWRT 400
    KKPVIFRATP QWFASISKVR QDILDAIENT NFKVNWGKTR IYNMVRDRGE 450
    WVISRQRVWG VPLPVFYAEN GEIIMTKETV NHVADLFAEH GSNIWFEREA 500
    KDLLPEGFTH PGSPNGTFTK ETDIMDVWFD SGSSHRGVLE TRPELSFPAD 550
    MYLEGSDQYR GWFNSSITTS VATRGVSPYK FLLSHGFVMD GEGKKMSKSL 600
    GNVIVPDQVV KQKGADIARL WVSSTDYLAD VRISDEILKQ TSDVYRKIRN 650
    TLRFMLGNIN DFNPDTDSIP ESELLEVDRY LLNRLREFTA STINNYENFD 700
    YLNIYQEVQN FINVELSNFY LDYGKDILYI EQRDSHIRRS MQTVLYQILV 750
    DMTKLLAPIL VHTAEEVWSH TPHVKEESVH LADMPKVVEV DQALLDKWRT 800
    FMNLRDDVNR ALETARNEKV IGKSLEAKVT IASNDKFNAS EFLTSFDALH 850
    QLFIVSQVKV VDKLDDQATA YEHGDIVIEH ADGEKCERCW NYSEDLGAVD 900
    ELTHLCPRCQ QVVKSLV 917
    Length:917
    Mass (Da):104,885
    Last modified:November 1, 1995 - v1
    Checksum:i8A2B644A03A85636
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74219 Genomic DNA. Translation: CAA52296.1.
    PIRiS40178.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74219 Genomic DNA. Translation: CAA52296.1 .
    PIRi S40178.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FFY X-ray 2.20 A 1-917 [» ]
    1QU2 X-ray 2.20 A 1-917 [» ]
    1QU3 X-ray 2.90 A 1-917 [» ]
    ProteinModelPortali P41972.
    SMRi P41972. Positions 1-917.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1982.
    DrugBanki DB00410. Mupirocin.

    Proteomic databases

    PRIDEi P41972.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P41972.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis and toxic overexpression in Escherichia coli of a staphylococcal gene encoding isoleucyl-tRNA synthetase."
      Chalker A.F., Ward J.M., Fosberry A.P., Hodgson J.E.
      Gene 141:103-108(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 9144 / Oxford / NCIB 6571 / NCTC 6571.
    2. "Insights into editing from an Ile-tRNA synthetase structure with tRNAIle and mupirocin."
      Silvian L.F., Wang J., Steitz T.A.
      Science 285:1074-1077(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH METAL IONS; ILE-TRNA AND MUPIROCIN.

    Entry informationi

    Entry nameiSYI1_STAAU
    AccessioniPrimary (citable) accession number: P41972
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3