P41970 (ELK3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ETS domain-containing protein Elk-3

Alternative name(s):
ETS-related protein ERP
ETS-related protein NET
Serum response factor accessory protein 2
Short name=SAP-2
Short name=SRF accessory protein 2

Gene names

Name:	ELK3
Synonyms:	NET, SAP2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element.
Subunit structure	Interacts with CTBP1.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: HGNC signal transduction Traceable author statement. Source: ProtInc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: HPA nucleus Inferred by curator. Source: HGNC
Molecular function	protein binding Inferred from physical interaction. Source: IntAct purine-rich negative regulatory element binding Inferred from direct assay. Source: HGNC sequence-specific DNA binding transcription factor activity Non-traceable author statement. Source: ProtInc transcription corepressor activity Inferred from direct assay. Source: HGNC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	3	EBI-1758534,EBI-389883

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	ETS domain-containing protein Elk-3		PRO_0000204097
Regions
	DNA binding	5 – 85	81	ETS
	Motif	273 – 277	5	CTBP-binding motif
	Compositional bias	207 – 212	6	Poly-Ala
Amino acid modifications
	Modified residue	115	1	Phosphoserine Ref.9
	Modified residue	245	1	Phosphoserine Ref.7
	Modified residue	396	1	Phosphoserine Ref.8
Natural variations
	Natural variant	169	1	P → L. Corresponds to variant rs35332676 [ dbSNP | Ensembl ].		VAR_048946
Experimental info
	Sequence conflict	114	1	A → V in CAA85309. Ref.1
	Sequence conflict	117	1	E → G in CAG47047. Ref.3
	Sequence conflict	128	1	A → V in CAA85309. Ref.1
	Sequence conflict	152	1	Q → E in CAA85309. Ref.1
	Sequence conflict	163	1	T → R in CAA85309. Ref.1
	Sequence conflict	249	1	N → K in CAA85309. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P41970 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: DD4515270ECED1E3	FASTA	407	44,240
	10 20 30 40 50 60 MESAITLWQF LLQLLLDQKH EHLICWTSND GEFKLLKAEE VAKLWGLRKN KTNMNYDKLS 70 80 90 100 110 120 RALRYYYDKN IIKKVIGQKF VYKFVSFPEI LKMDPHAVEI SRESLLLQDS DCKASPEGRE 130 140 150 160 170 180 AHKHGLAALR STSRNEYIHS GLYSSFTINS LQNPPDAFKA IKTEKLEEPP EDSPPVEEVR 190 200 210 220 230 240 TVIRFVTNKT DKHVTRPVVS LPSTSEAAAA SAFLASSVSA KISSLMLPNA ASISSASPFS 250 260 270 280 290 300 SRSPSLSPNS PLPSEHRSLF LEAACHDSDS LEPLNLSSGS KTKSPSLPPK AKKPKGLEIS 310 320 330 340 350 360 APPLVLSGTD IGSIALNSPA LPSGSLTPAF FTAQTPNGLL LTPSPLLSSI HFWSSLSPVA 370 380 390 400 PLSPARLQGP STLFQFPTLL NGHMPVPIPS LDRAASPVLL SSNSQKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Net, a new ets transcription factor that is activated by Ras." Giovane A., Pintzas A., Maira S.-M., Sobieszczuk P., Wasylyk B. Genes Dev. 8:1502-1513(1994) [PubMed: 7958835] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Comparative analysis of the ternary complex factors Elk-1, SAP-1a and SAP-2 (ERP/NET)." Price M.A., Rogers A.E., Treisman R. EMBO J. 14:2589-2601(1995) [PubMed: 7540136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-396, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36715 mRNA. Translation: CAA85309.1. CR542251 mRNA. Translation: CAG47047.1. AK312694 mRNA. Translation: BAG35573.1. CH471054 Genomic DNA. Translation: EAW97561.1. BC017371 mRNA. Translation: AAH17371.1.
IPI	IPI00018266.
PIR	I38062.
RefSeq	NP_005221.2. NM_005230.2.
UniGene	Hs.46523.
3D structure databases
ProteinModelPortal	P41970.
SMR	P41970. Positions 2-151.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41970. 3 interactions.
MINT	MINT-7242082.
STRING	P41970.
PTM databases
PhosphoSite	P41970.
Polymorphism databases
DMDM	116241349.
Proteomic databases
PRIDE	P41970.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000228741; ENSP00000228741; ENSG00000111145.
GeneID	2004.
KEGG	hsa:2004.
UCSC	uc001teo.1. human.
Organism-specific databases
CTD	2004.
GeneCards	GC12P096521.
H-InvDB	HIX0010903.
HGNC	HGNC:3325. ELK3.
HPA	HPA001600.
MIM	600247. gene.
neXtProt	NX_P41970.
PharmGKB	PA27752.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04021.
GeneTree	ENSGT00600000083997.
HOGENOM	HBG716750.
HOVERGEN	HBG004344.
InParanoid	P41970.
OMA	NKTDKHV.
OrthoDB	EOG4TMR2X.
PhylomeDB	P41970.
Gene expression databases
ArrayExpress	P41970.
Bgee	P41970.
CleanEx	HS_ELK3.
Genevestigator	P41970.
GermOnline	ENSG00000111145. Homo sapiens.
Family and domain databases
InterPro	IPR000418. Ets. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K09430.
Pfam	PF00178. Ets. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	8109.
SOURCE	Search...

Entry information

Entry name

ELK3_HUMAN

Accession

Primary (citable) accession number: P41970
Secondary accession number(s): B2R6S6 , Q6FG57, Q6GU29, Q9UD17

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.