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Protein

ETS domain-containing protein Elk-1

Gene

Elk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2 (By similarity). Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi5 – 86ETSPROSITE-ProRule annotationAdd BLAST82

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-198753. ERK/MAPK targets.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS domain-containing protein Elk-1
Gene namesi
Name:Elk1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:101833. Elk1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002040961 – 429ETS domain-containing protein Elk-1Add BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki231Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki250Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki255Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei325Phosphoserine; by MAPK1By similarity1
Modified residuei337Phosphothreonine; by MAPK1By similarity1
Modified residuei354Phosphothreonine; by MAPK1By similarity1
Modified residuei364Phosphothreonine; by MAPK1By similarity1
Modified residuei369Phosphothreonine; by MAPK1By similarity1
Modified residuei384Phosphoserine; by MAPK1 and MAPK8By similarity1
Modified residuei390Phosphoserine; by MAPK1By similarity1
Modified residuei418Phosphothreonine; by MAPK1By similarity1
Modified residuei423Phosphoserine; by MAPK1By similarity1

Post-translational modificationi

Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention (By similarity).By similarity
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1 but also MAPK8 and/or MAPK9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CaMK4, MAPK11, MAPK12 and MAPK14 (By similarity). Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP41969.
PaxDbiP41969.
PRIDEiP41969.

PTM databases

iPTMnetiP41969.
PhosphoSitePlusiP41969.

Expressioni

Tissue specificityi

Predominantly expressed in the brain, and to a lesser extent in the heart, liver and muscle.

Gene expression databases

BgeeiENSMUSG00000009406.
CleanExiMM_ELK1.
GenevisibleiP41969. MM.

Interactioni

Subunit structurei

Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9. Interacts with POU1F1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199429. 2 interactors.
DIPiDIP-61141N.
STRINGi10090.ENSMUSP00000009550.

Structurei

3D structure databases

ProteinModelPortaliP41969.
SMRiP41969.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni350 – 400Sufficient for interaction with MAD2L2By similarityAdd BLAST51

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000237332.
HOVERGENiHBG004344.
InParanoidiP41969.
KOiK04375.
OMAiKSEEPNM.
OrthoDBiEOG091G0CL4.
TreeFamiTF317732.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPSVTLWQF LLQLLREQGN GHIISWTSRD GGEFKLVDAE EVARLWGLRK
60 70 80 90 100
NKTNMNYDKL SRALRYYYDK NIIRKVSGQK FVYKFVSYPE VAGCSTEDCP
110 120 130 140 150
PQPEVSVTSA IAMAPATVHA GPGDTATGKP GTPKGAGMTG QGGLARSSRN
160 170 180 190 200
EYMRSGLYST FTIQSLQPQP QPPIPPRPAS VLPNTTPAGV PAPASGSRST
210 220 230 240 250
SPNPLEACLE AEEAGLPLQV ILTPPEAPNQ KSEELSLDPS FGHPQPPEVK
260 270 280 290 300
VEGPKEELEA ARAGGFSSEA VKAEPEVSAS EGLLARLPAI LTENTAQVCG
310 320 330 340 350
LSTSTTEITQ PQKGRKPRDL ELPLSPSLLG GQGPERTPGS GTSSGLQAPG
360 370 380 390 400
PALTPSLLPT HTLTPVLLTP SSLPPSIHFW STLSPIAPRS PAKLSFQFPS
410 420
SGSAQVHIPS ISVDGLSTPV VLSPGPQKP
Length:429
Mass (Da):45,271
Last modified:July 27, 2011 - v3
Checksum:i2E1C5B80790C6DA7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133P → T in CAA85391 (PubMed:7958835).Curated1
Sequence conflicti249V → A in CAA60715 (PubMed:8863747).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87257 mRNA. Translation: CAA60715.1.
AK132354 mRNA. Translation: BAE21121.1.
AL671853 Genomic DNA. Translation: CAM19337.1.
CH466625 Genomic DNA. Translation: EDL00733.1.
Z36939 mRNA. Translation: CAA85391.1.
CCDSiCCDS30048.1.
PIRiJC4965.
RefSeqiNP_031948.4. NM_007922.5.
UniGeneiMm.405823.
Mm.490895.

Genome annotation databases

EnsembliENSMUST00000009550; ENSMUSP00000009550; ENSMUSG00000009406.
GeneIDi13712.
KEGGimmu:13712.
UCSCiuc009suc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87257 mRNA. Translation: CAA60715.1.
AK132354 mRNA. Translation: BAE21121.1.
AL671853 Genomic DNA. Translation: CAM19337.1.
CH466625 Genomic DNA. Translation: EDL00733.1.
Z36939 mRNA. Translation: CAA85391.1.
CCDSiCCDS30048.1.
PIRiJC4965.
RefSeqiNP_031948.4. NM_007922.5.
UniGeneiMm.405823.
Mm.490895.

3D structure databases

ProteinModelPortaliP41969.
SMRiP41969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199429. 2 interactors.
DIPiDIP-61141N.
STRINGi10090.ENSMUSP00000009550.

PTM databases

iPTMnetiP41969.
PhosphoSitePlusiP41969.

Proteomic databases

MaxQBiP41969.
PaxDbiP41969.
PRIDEiP41969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009550; ENSMUSP00000009550; ENSMUSG00000009406.
GeneIDi13712.
KEGGimmu:13712.
UCSCiuc009suc.2. mouse.

Organism-specific databases

CTDi2002.
MGIiMGI:101833. Elk1.

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000237332.
HOVERGENiHBG004344.
InParanoidiP41969.
KOiK04375.
OMAiKSEEPNM.
OrthoDBiEOG091G0CL4.
TreeFamiTF317732.

Enzyme and pathway databases

ReactomeiR-MMU-198753. ERK/MAPK targets.

Miscellaneous databases

PROiP41969.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000009406.
CleanExiMM_ELK1.
GenevisibleiP41969. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELK1_MOUSE
AccessioniPrimary (citable) accession number: P41969
Secondary accession number(s): Q3V1M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.