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Protein

ETS domain-containing protein Elk-1

Gene

Elk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi5 – 8682ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_323000. ERK/MAPK targets.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS domain-containing protein Elk-1
Gene namesi
Name:Elk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:101833. Elk1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429ETS domain-containing protein Elk-1PRO_0000204096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki231 – 231Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki250 – 250Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki255 – 255Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei325 – 3251Phosphoserine; by MAPK1By similarity
Modified residuei337 – 3371Phosphothreonine; by MAPK1By similarity
Modified residuei354 – 3541Phosphothreonine; by MAPK1By similarity
Modified residuei364 – 3641Phosphothreonine; by MAPK1By similarity
Modified residuei369 – 3691Phosphothreonine; by MAPK1By similarity
Modified residuei384 – 3841Phosphoserine; by MAPK1 and MAPK8By similarity
Modified residuei390 – 3901Phosphoserine; by MAPK1By similarity
Modified residuei418 – 4181Phosphothreonine; by MAPK1By similarity
Modified residuei423 – 4231Phosphoserine; by MAPK1By similarity

Post-translational modificationi

Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention (By similarity).By similarity
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1 but also MAPK8 and/or MAPK9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CaMK4, MAPK11, MAPK12 and MAPK14 (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP41969.

PTM databases

PhosphoSiteiP41969.

Expressioni

Tissue specificityi

Predominantly expressed in the brain, and to a lesser extent in the heart, liver and muscle.

Gene expression databases

BgeeiP41969.
CleanExiMM_ELK1.
ExpressionAtlasiP41969. baseline and differential.
GenevisibleiP41969. MM.

Interactioni

Subunit structurei

Interacts in its sumoylated form with PIAS2 which enhances its transcriptional activator activity. Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9 (By similarity).By similarity

Protein-protein interaction databases

BioGridi199429. 2 interactions.
DIPiDIP-61141N.
STRINGi10090.ENSMUSP00000009550.

Structurei

3D structure databases

ProteinModelPortaliP41969.
SMRiP41969. Positions 5-90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni350 – 40051Sufficient for interaction with MAD2L2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG269367.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000237332.
HOVERGENiHBG004344.
InParanoidiP41969.
KOiK04375.
OMAiMDTAGQA.
OrthoDBiEOG7NPFTD.
TreeFamiTF317732.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPSVTLWQF LLQLLREQGN GHIISWTSRD GGEFKLVDAE EVARLWGLRK
60 70 80 90 100
NKTNMNYDKL SRALRYYYDK NIIRKVSGQK FVYKFVSYPE VAGCSTEDCP
110 120 130 140 150
PQPEVSVTSA IAMAPATVHA GPGDTATGKP GTPKGAGMTG QGGLARSSRN
160 170 180 190 200
EYMRSGLYST FTIQSLQPQP QPPIPPRPAS VLPNTTPAGV PAPASGSRST
210 220 230 240 250
SPNPLEACLE AEEAGLPLQV ILTPPEAPNQ KSEELSLDPS FGHPQPPEVK
260 270 280 290 300
VEGPKEELEA ARAGGFSSEA VKAEPEVSAS EGLLARLPAI LTENTAQVCG
310 320 330 340 350
LSTSTTEITQ PQKGRKPRDL ELPLSPSLLG GQGPERTPGS GTSSGLQAPG
360 370 380 390 400
PALTPSLLPT HTLTPVLLTP SSLPPSIHFW STLSPIAPRS PAKLSFQFPS
410 420
SGSAQVHIPS ISVDGLSTPV VLSPGPQKP
Length:429
Mass (Da):45,271
Last modified:July 27, 2011 - v3
Checksum:i2E1C5B80790C6DA7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331P → T in CAA85391 (PubMed:7958835).Curated
Sequence conflicti249 – 2491V → A in CAA60715 (PubMed:8863747).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87257 mRNA. Translation: CAA60715.1.
AK132354 mRNA. Translation: BAE21121.1.
AL671853 Genomic DNA. Translation: CAM19337.1.
CH466625 Genomic DNA. Translation: EDL00733.1.
Z36939 mRNA. Translation: CAA85391.1.
CCDSiCCDS30048.1.
PIRiJC4965.
RefSeqiNP_031948.4. NM_007922.5.
UniGeneiMm.405823.
Mm.490895.

Genome annotation databases

EnsembliENSMUST00000009550; ENSMUSP00000009550; ENSMUSG00000009406.
GeneIDi13712.
KEGGimmu:13712.
UCSCiuc009suc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87257 mRNA. Translation: CAA60715.1.
AK132354 mRNA. Translation: BAE21121.1.
AL671853 Genomic DNA. Translation: CAM19337.1.
CH466625 Genomic DNA. Translation: EDL00733.1.
Z36939 mRNA. Translation: CAA85391.1.
CCDSiCCDS30048.1.
PIRiJC4965.
RefSeqiNP_031948.4. NM_007922.5.
UniGeneiMm.405823.
Mm.490895.

3D structure databases

ProteinModelPortaliP41969.
SMRiP41969. Positions 5-90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199429. 2 interactions.
DIPiDIP-61141N.
STRINGi10090.ENSMUSP00000009550.

PTM databases

PhosphoSiteiP41969.

Proteomic databases

PRIDEiP41969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009550; ENSMUSP00000009550; ENSMUSG00000009406.
GeneIDi13712.
KEGGimmu:13712.
UCSCiuc009suc.2. mouse.

Organism-specific databases

CTDi2002.
MGIiMGI:101833. Elk1.

Phylogenomic databases

eggNOGiNOG269367.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000237332.
HOVERGENiHBG004344.
InParanoidiP41969.
KOiK04375.
OMAiMDTAGQA.
OrthoDBiEOG7NPFTD.
TreeFamiTF317732.

Enzyme and pathway databases

ReactomeiREACT_323000. ERK/MAPK targets.

Miscellaneous databases

NextBioi284482.
PROiP41969.
SOURCEiSearch...

Gene expression databases

BgeeiP41969.
CleanExiMM_ELK1.
ExpressionAtlasiP41969. baseline and differential.
GenevisibleiP41969. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Net, a new ets transcription factor that is activated by Ras."
    Giovane A., Pintzas A., Maira S.-M., Sobieszczuk P., Wasylyk B.
    Genes Dev. 8:1502-1513(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-224.
    Tissue: Embryo.

Entry informationi

Entry nameiELK1_MOUSE
AccessioniPrimary (citable) accession number: P41969
Secondary accession number(s): Q3V1M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.