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Protein

Drosomycin

Gene

Drs

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses antifungal activity and is active against a relatively broad spectrum of filamentous fungi. It inhibits spore germination at high concentrations and at low concentrations delays growth of hyphae which subsequently exhibit abnormal morphology. Spz C-106 in the hemolymph controls expression of the antifungal peptide by acting as a ligand of Tl and inducing an intracellular signaling pathway.2 Publications

GO - Biological processi

  • antibacterial humoral response Source: FlyBase
  • antifungal humoral response Source: FlyBase
  • cytolysis in other organism Source: FlyBase
  • defense response Source: FlyBase
  • defense response to fungus Source: FlyBase
  • defense response to Gram-negative bacterium Source: FlyBase
  • defense response to protozoan Source: FlyBase
  • innate immune response Source: FlyBase
  • response to bacterium Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Protein family/group databases

TCDBi8.B.1.1.9. the long (4c-c) scorpion toxin (l-st) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Drosomycin
Alternative name(s):
Cysteine-rich peptide
Gene namesi
Name:Drs
Synonyms:CRP
ORF Names:CG10810
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0283461. Drs.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 2661 PublicationPRO_0000007038
Chaini27 – 7044DrosomycinPRO_0000007039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 70
Disulfide bondi37 ↔ 59
Disulfide bondi45 ↔ 65
Disulfide bondi49 ↔ 67

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP41964.
PRIDEiP41964.

Expressioni

Tissue specificityi

Synthesized in the fat body and is secreted into the blood. In hemolymph 6 hours after immune challenge, levels of expression increase for first 24 hours and persist for the following three weeks.1 Publication

Gene expression databases

BgeeiP41964.
GenevisibleiP41964. DM.

Interactioni

Protein-protein interaction databases

DIPiDIP-18879N.
MINTiMINT-291861.
STRINGi7227.FBpp0072935.

Structurei

Secondary structure

1
70
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni39 – 413Combined sources
Helixi42 – 5211Combined sources
Beta strandi54 – 607Combined sources
Turni61 – 633Combined sources
Beta strandi64 – 685Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MYNNMR-A27-70[»]
ProteinModelPortaliP41964.
SMRiP41964. Positions 27-70.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41964.

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00840000130934.
InParanoidiP41964.
OMAiHETCRRV.
OrthoDBiEOG75F4GX.
PhylomeDBiP41964.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41964-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQIKYLFAL FAVLMLVVLG ANEADADCLS GRYKGPCAVW DNETCRRVCK
60 70
EEGRSSGHCS PSLKCWCEGC
Length:70
Mass (Da):7,752
Last modified:November 1, 1995 - v1
Checksum:i1A702E959ECEF181
GO

Mass spectrometryi

Molecular mass is 4889.5 Da from positions 27 - 70. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75595 mRNA. Translation: CAA53267.1.
AJ885056 Genomic DNA. Translation: CAI76995.1.
AJ885057 Genomic DNA. Translation: CAI77002.1.
AJ885058 Genomic DNA. Translation: CAI77009.1.
AJ885059 Genomic DNA. Translation: CAI77016.1.
AJ885060 Genomic DNA. Translation: CAI77023.1.
AJ885061 Genomic DNA. Translation: CAI77030.1.
AJ885062 Genomic DNA. Translation: CAI77037.1.
AJ885063 Genomic DNA. Translation: CAI77044.1.
AJ885064 Genomic DNA. Translation: CAI77051.1.
AJ885065 Genomic DNA. Translation: CAI77058.1.
AJ885066 Genomic DNA. Translation: CAI77065.1.
AJ885067 Genomic DNA. Translation: CAI77072.1.
AJ885068 Genomic DNA. Translation: CAI77079.1.
AJ885069 Genomic DNA. Translation: CAI77086.1.
AJ885070 Genomic DNA. Translation: CAI77093.1.
AJ885071 Genomic DNA. Translation: CAI77100.1.
AJ885072 Genomic DNA. Translation: CAI77107.1.
AJ885073 Genomic DNA. Translation: CAI77114.1.
AJ885074 Genomic DNA. Translation: CAI77121.1.
AJ885075 Genomic DNA. Translation: CAI77128.1.
AJ885086 Genomic DNA. Translation: CAI77205.1.
AE014296 Genomic DNA. Translation: AAF47767.2.
AY119009 mRNA. Translation: AAM50869.1.
PIRiA55824.
RefSeqiNP_523901.1. NM_079177.4.
UniGeneiDm.14479.

Genome annotation databases

EnsemblMetazoaiFBtr0073073; FBpp0072935; FBgn0010381.
GeneIDi38419.
KEGGidme:Dmel_CG10810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75595 mRNA. Translation: CAA53267.1.
AJ885056 Genomic DNA. Translation: CAI76995.1.
AJ885057 Genomic DNA. Translation: CAI77002.1.
AJ885058 Genomic DNA. Translation: CAI77009.1.
AJ885059 Genomic DNA. Translation: CAI77016.1.
AJ885060 Genomic DNA. Translation: CAI77023.1.
AJ885061 Genomic DNA. Translation: CAI77030.1.
AJ885062 Genomic DNA. Translation: CAI77037.1.
AJ885063 Genomic DNA. Translation: CAI77044.1.
AJ885064 Genomic DNA. Translation: CAI77051.1.
AJ885065 Genomic DNA. Translation: CAI77058.1.
AJ885066 Genomic DNA. Translation: CAI77065.1.
AJ885067 Genomic DNA. Translation: CAI77072.1.
AJ885068 Genomic DNA. Translation: CAI77079.1.
AJ885069 Genomic DNA. Translation: CAI77086.1.
AJ885070 Genomic DNA. Translation: CAI77093.1.
AJ885071 Genomic DNA. Translation: CAI77100.1.
AJ885072 Genomic DNA. Translation: CAI77107.1.
AJ885073 Genomic DNA. Translation: CAI77114.1.
AJ885074 Genomic DNA. Translation: CAI77121.1.
AJ885075 Genomic DNA. Translation: CAI77128.1.
AJ885086 Genomic DNA. Translation: CAI77205.1.
AE014296 Genomic DNA. Translation: AAF47767.2.
AY119009 mRNA. Translation: AAM50869.1.
PIRiA55824.
RefSeqiNP_523901.1. NM_079177.4.
UniGeneiDm.14479.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MYNNMR-A27-70[»]
ProteinModelPortaliP41964.
SMRiP41964. Positions 27-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-18879N.
MINTiMINT-291861.
STRINGi7227.FBpp0072935.

Protein family/group databases

TCDBi8.B.1.1.9. the long (4c-c) scorpion toxin (l-st) superfamily.

Proteomic databases

PaxDbiP41964.
PRIDEiP41964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073073; FBpp0072935; FBgn0010381.
GeneIDi38419.
KEGGidme:Dmel_CG10810.

Organism-specific databases

CTDi38419.
FlyBaseiFBgn0283461. Drs.

Phylogenomic databases

GeneTreeiENSGT00840000130934.
InParanoidiP41964.
OMAiHETCRRV.
OrthoDBiEOG75F4GX.
PhylomeDBiP41964.

Miscellaneous databases

EvolutionaryTraceiP41964.
GenomeRNAii38419.
NextBioi808566.
PROiP41964.

Gene expression databases

BgeeiP41964.
GenevisibleiP41964. DM.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Insect immunity. Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides."
    Fehlbaum P., Bulet P., Michaut L., Lagueux M., Broekaert W.F., Hetru C., Hoffmann J.A.
    J. Biol. Chem. 269:33159-33163(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-70.
    Strain: Oregon-R.
  2. "The evolution of antifungal peptides in Drosophila."
    Jiggins F.M., Kim K.W.
    Genetics 171:1847-1859(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: G125, G128, G130, G140, G141, G179, G185, G187, KY258, N01, N02, N03, N06, N07, N14, N15, N16, N17, N22, N29 and N30.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Larva and Pupae.
  6. "The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults."
    Lemaitre B., Nicolas E., Michaut L., Reichhart J.-M., Hoffmann J.A.
    Cell 86:973-983(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Differential display of peptides induced during the immune response of Drosophila: a matrix-assisted laser desorption ionization time-of-flight mass spectrometry study."
    Uttenweiler-Joseph S., Moniatte M., Lagueux M., van Dorsselaer A., Hoffmann J.A., Bulet P.
    Proc. Natl. Acad. Sci. U.S.A. 95:11342-11347(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY, INDUCTION, TISSUE SPECIFICITY.
  8. "Binding of the Drosophila cytokine Spatzle to Toll is direct and establishes signaling."
    Weber A.N., Tauszig-Delamasure S., Hoffmann J.A., Lelievre E., Gascan H., Ray K.P., Morse M.A., Imler J.L., Gay N.J.
    Nat. Immunol. 4:794-800(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Solution structure of drosomycin, the first inducible antifungal protein from insects."
    Landon C., Sodano P., Hetru C., Hoffmann J.A., Ptak M.
    Protein Sci. 6:1878-1884(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiDMYC_DROME
AccessioniPrimary (citable) accession number: P41964
Secondary accession number(s): Q2UYN5, Q9VZQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.