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P41948 (MEP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ammonium transporter MEP2
Gene names
Name:MEP2
Synonyms:AMT2
Ordered Locus Names:YNL142W
ORF Names:N1207, N1820
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth. Ref.1 Ref.5 Ref.6 Ref.7

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ammonia transporter channel (TC 1.A.11.2) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Ammonium transporter MEP2
PRO_0000139755

Regions

Topological domain1 – 3131Extracellular Potential
Transmembrane32 – 5221Helical; Potential
Topological domain53 – 6210Cytoplasmic Potential
Transmembrane63 – 8321Helical; Potential
Topological domain84 – 12239Extracellular Potential
Transmembrane123 – 14321Helical; Potential
Topological domain144 – 1529Cytoplasmic Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 18714Extracellular Potential
Transmembrane188 – 20821Helical; Potential
Topological domain209 – 23022Cytoplasmic Potential
Transmembrane231 – 25121Helical; Potential
Topological domain252 – 2576Extracellular Potential
Transmembrane258 – 27821Helical; Potential
Topological domain279 – 30022Cytoplasmic Potential
Transmembrane301 – 32121Helical; Potential
Topological domain322 – 34625Extracellular Potential
Transmembrane347 – 36721Helical; Potential
Topological domain368 – 39326Cytoplasmic Potential
Transmembrane394 – 41421Helical; Potential
Topological domain415 – 49985Extracellular Potential

Amino acid modifications

Glycosylation41N-linked (GlcNAc...) Ref.6

Experimental info

Mutagenesis41N → Q: Molecular weight equal to non-glycosylated form. Ref.6
Mutagenesis2521N → Q: Molecular weight similar to wild-type glycosylated form. Ref.6
Mutagenesis3681N → Q: Molecular weight similar to wild-type glycosylated form. Ref.6
Mutagenesis4831N → Q: Molecular weight similar to wild-type glycosylated form. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P41948 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 9E81D08018B68D94

FASTA49953,401
        10         20         30         40         50         60 
MSYNFTGTPT GEGTGGNSLT TDLNTQFDLA NMGWIGVASA GVWIMVPGIG LLYSGLSRKK 

        70         80         90        100        110        120 
HALSLLWASM MASAVCIFQW FFWGYSLAFS HNTRGNGFIG TLEFFGFRNV LGAPSSVSSL 

       130        140        150        160        170        180 
PDILFAVYQG MFAAVTGALM LGGACERARL FPMMVFLFLW MTIVYCPIAC WVWNAEGWLV 

       190        200        210        220        230        240 
KLGSLDYAGG LCVHLTSGHG GLVYALILGK RNDPVTRKGM PKYKPHSVTS VVLGTVFLWF 

       250        260        270        280        290        300 
GWMFFNGGSA GNATIRAWYS IMSTNLAAAC GGLTWMVIDY FRCGRKWTTV GLCSGIIAGL 

       310        320        330        340        350        360 
VGITPAAGFV PIWSAVVIGV VTGAGCNLAV DLKSLLRIDD GLDCYSIHGV GGCIGSVLTG 

       370        380        390        400        410        420 
IFAADYVNAT AGSYISPIDG GWINHHYKQV GYQLAGICAA LAWTVTVTSI LLLTMNAIPF 

       430        440        450        460        470        480 
LKLRLSADEE ELGTDAAQIG EFTYEESTAY IPEPIRSKTS AQMPPPHENI DDKIVGNTDA 

       490 
EKNSTPSDAS STKNTDHIV

« Hide

References

« Hide 'large scale' references
[1]"A family of ammonium transporters in Saccharomyces cerevisiae."
Marini A.-M., Soussi-Boudekou S., Vissers S., Andre B.
Mol. Cell. Biol. 17:4282-4293(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: Sigma 1278B.
[2]"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
Mallet L., Bussereau F., Jacquet M.
Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The MEP2 ammonium permease regulates pseudohyphal differentiation in Saccharomyces cerevisiae."
Lorenz M.C., Heitman J.
EMBO J. 17:1236-1247(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: Sigma 1278B.
[6]"In vivo N-glycosylation of the mep2 high-affinity ammonium transporter of Saccharomyces cerevisiae reveals an extracytosolic N-terminus."
Marini A.-M., Andre B.
Mol. Microbiol. 38:552-564(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TOPOLOGY, MUTAGENESIS OF ASN-4; ASN-252; ASN-368 AND ASN-483, GLYCOSYLATION AT ASN-4.
Strain: Sigma 1278B.
[7]"Evidence that fungal MEP proteins mediate diffusion of the uncharged species NH(3) across the cytoplasmic membrane."
Soupene E., Ramirez R.M., Kustu S.
Mol. Cell. Biol. 21:5733-5741(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X83608 Genomic DNA. Translation: CAA58587.1.
Z46843 Genomic DNA. Translation: CAA86884.1.
Z71418 Genomic DNA. Translation: CAA96025.1.
BK006947 Genomic DNA. Translation: DAA10406.1.
PIRS51089.
RefSeqNP_014257.1. NM_001182980.1.

3D structure databases

ProteinModelPortalP41948.
SMRP41948. Positions 30-444.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4340N.
IntActP41948. 1 interaction.
MINTMINT-568645.
STRING4932.YNL142W.

Protein family/group databases

TCDB1.A.11.3.2. ammonia transporter channel (Amt) family.

Proteomic databases

PaxDbP41948.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL142W; YNL142W; YNL142W.
GeneID855580.
KEGGsce:YNL142W.

Organism-specific databases

CYGDYNL142w.
SGDS000005086. MEP2.

Phylogenomic databases

eggNOGCOG0004.
GeneTreeENSGT00530000064546.
HOGENOMHOG000017736.
KOK03320.
OMAFAMITPA.
OrthoDBEOG49W5PW.

Gene expression databases

GenevestigatorP41948.
GermOnlineYNL142W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERPTHR11730. PTHR11730. 1 hit.
PfamPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMSSF111352. RH_like_transpt. 1 hit.
TIGRFAMsTIGR00836. amt. 1 hit.
PROSITEPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP41948.
ChEMBLCHEMBL1741183.
NextBio979706.

Entry information

Entry nameMEP2_YEAST
AccessionPrimary (citable) accession number: P41948
Secondary accession number(s): D6W140
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents