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Protein

Ammonium transporter MEP2

Gene

MEP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Under ammonium limitation acts as an ammonium sensor, generating a signal that leads to pseudohyphal growth.4 Publications

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: SGD

GO - Biological processi

  • ammonium transport Source: SGD
  • nitrogen utilization Source: SGD
  • organic cation transport Source: GO_Central
  • pseudohyphal growth Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ammonia transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33160-MONOMER.

Protein family/group databases

TCDBi1.A.11.3.2. the ammonium transporter channel (amt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonium transporter MEP2
Gene namesi
Name:MEP2
Synonyms:AMT2
Ordered Locus Names:YNL142W
ORF Names:N1207, N1820
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL142W.
SGDiS000005086. MEP2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31ExtracellularSequence analysisAdd BLAST31
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Topological domaini53 – 62CytoplasmicSequence analysis10
Transmembranei63 – 83HelicalSequence analysisAdd BLAST21
Topological domaini84 – 122ExtracellularSequence analysisAdd BLAST39
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 152CytoplasmicSequence analysis9
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 187ExtracellularSequence analysisAdd BLAST14
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Topological domaini209 – 230CytoplasmicSequence analysisAdd BLAST22
Transmembranei231 – 251HelicalSequence analysisAdd BLAST21
Topological domaini252 – 257ExtracellularSequence analysis6
Transmembranei258 – 278HelicalSequence analysisAdd BLAST21
Topological domaini279 – 300CytoplasmicSequence analysisAdd BLAST22
Transmembranei301 – 321HelicalSequence analysisAdd BLAST21
Topological domaini322 – 346ExtracellularSequence analysisAdd BLAST25
Transmembranei347 – 367HelicalSequence analysisAdd BLAST21
Topological domaini368 – 393CytoplasmicSequence analysisAdd BLAST26
Transmembranei394 – 414HelicalSequence analysisAdd BLAST21
Topological domaini415 – 499ExtracellularSequence analysisAdd BLAST85

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4N → Q: Molecular weight equal to non-glycosylated form. 1 Publication1
Mutagenesisi252N → Q: Molecular weight similar to wild-type glycosylated form. 1 Publication1
Mutagenesisi368N → Q: Molecular weight similar to wild-type glycosylated form. 1 Publication1
Mutagenesisi483N → Q: Molecular weight similar to wild-type glycosylated form. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1741183.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001397551 – 499Ammonium transporter MEP2Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi4N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP41948.

PTM databases

iPTMnetiP41948.

Interactioni

Protein-protein interaction databases

BioGridi35685. 45 interactors.
DIPiDIP-4340N.
IntActiP41948. 1 interactor.
MINTiMINT-568645.

Chemistry databases

BindingDBiP41948.

Structurei

Secondary structure

1499
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 26Combined sources4
Helixi29 – 43Combined sources15
Helixi45 – 55Combined sources11
Helixi65 – 82Combined sources18
Helixi84 – 89Combined sources6
Beta strandi91 – 100Combined sources10
Helixi105 – 107Combined sources3
Beta strandi111 – 114Combined sources4
Helixi122 – 142Combined sources21
Helixi150 – 163Combined sources14
Helixi165 – 172Combined sources8
Turni178 – 183Combined sources6
Turni190 – 193Combined sources4
Helixi194 – 207Combined sources14
Helixi214 – 216Combined sources3
Helixi228 – 247Combined sources20
Helixi248 – 250Combined sources3
Beta strandi251 – 254Combined sources4
Helixi255 – 282Combined sources28
Helixi291 – 302Combined sources12
Turni303 – 309Combined sources7
Helixi314 – 330Combined sources17
Helixi333 – 336Combined sources4
Helixi342 – 362Combined sources21
Helixi365 – 368Combined sources4
Helixi369 – 374Combined sources6
Helixi382 – 384Combined sources3
Helixi389 – 415Combined sources27
Turni419 – 421Combined sources3
Turni427 – 429Combined sources3
Helixi436 – 438Combined sources3
Beta strandi441 – 444Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AEXX-ray3.20A/B/C/D/E/F/H/I/J1-499[»]
ProteinModelPortaliP41948.
SMRiP41948.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP41948.
KOiK03320.
OMAiCVLWVVY.
OrthoDBiEOG092C1Z22.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYNFTGTPT GEGTGGNSLT TDLNTQFDLA NMGWIGVASA GVWIMVPGIG
60 70 80 90 100
LLYSGLSRKK HALSLLWASM MASAVCIFQW FFWGYSLAFS HNTRGNGFIG
110 120 130 140 150
TLEFFGFRNV LGAPSSVSSL PDILFAVYQG MFAAVTGALM LGGACERARL
160 170 180 190 200
FPMMVFLFLW MTIVYCPIAC WVWNAEGWLV KLGSLDYAGG LCVHLTSGHG
210 220 230 240 250
GLVYALILGK RNDPVTRKGM PKYKPHSVTS VVLGTVFLWF GWMFFNGGSA
260 270 280 290 300
GNATIRAWYS IMSTNLAAAC GGLTWMVIDY FRCGRKWTTV GLCSGIIAGL
310 320 330 340 350
VGITPAAGFV PIWSAVVIGV VTGAGCNLAV DLKSLLRIDD GLDCYSIHGV
360 370 380 390 400
GGCIGSVLTG IFAADYVNAT AGSYISPIDG GWINHHYKQV GYQLAGICAA
410 420 430 440 450
LAWTVTVTSI LLLTMNAIPF LKLRLSADEE ELGTDAAQIG EFTYEESTAY
460 470 480 490
IPEPIRSKTS AQMPPPHENI DDKIVGNTDA EKNSTPSDAS STKNTDHIV
Length:499
Mass (Da):53,401
Last modified:November 1, 1995 - v1
Checksum:i9E81D08018B68D94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83608 Genomic DNA. Translation: CAA58587.1.
Z46843 Genomic DNA. Translation: CAA86884.1.
Z71418 Genomic DNA. Translation: CAA96025.1.
BK006947 Genomic DNA. Translation: DAA10406.1.
PIRiS51089.
RefSeqiNP_014257.1. NM_001182980.1.

Genome annotation databases

EnsemblFungiiYNL142W; YNL142W; YNL142W.
GeneIDi855580.
KEGGisce:YNL142W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83608 Genomic DNA. Translation: CAA58587.1.
Z46843 Genomic DNA. Translation: CAA86884.1.
Z71418 Genomic DNA. Translation: CAA96025.1.
BK006947 Genomic DNA. Translation: DAA10406.1.
PIRiS51089.
RefSeqiNP_014257.1. NM_001182980.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AEXX-ray3.20A/B/C/D/E/F/H/I/J1-499[»]
ProteinModelPortaliP41948.
SMRiP41948.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35685. 45 interactors.
DIPiDIP-4340N.
IntActiP41948. 1 interactor.
MINTiMINT-568645.

Chemistry databases

BindingDBiP41948.
ChEMBLiCHEMBL1741183.

Protein family/group databases

TCDBi1.A.11.3.2. the ammonium transporter channel (amt) family.

PTM databases

iPTMnetiP41948.

Proteomic databases

PRIDEiP41948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL142W; YNL142W; YNL142W.
GeneIDi855580.
KEGGisce:YNL142W.

Organism-specific databases

EuPathDBiFungiDB:YNL142W.
SGDiS000005086. MEP2.

Phylogenomic databases

GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP41948.
KOiK03320.
OMAiCVLWVVY.
OrthoDBiEOG092C1Z22.

Enzyme and pathway databases

BioCyciYEAST:G3O-33160-MONOMER.

Miscellaneous databases

PROiP41948.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEP2_YEAST
AccessioniPrimary (citable) accession number: P41948
Secondary accession number(s): D6W140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.