P41946 (MEL5_YEASX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-galactosidase 5
EC=3.2.1.22

Alternative name(s):
Alpha-D-galactoside galactohydrolase 5
Melibiase 5

Gene names

Name:

MEL5

Organism

Saccharomyces cerevisiae (Baker's yeast)

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subunit structure	Homotetramer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Chain	19 – 471	453	Alpha-galactosidase 5		PRO_0000001015
Sites
	Active site	149	1	Nucleophile By similarity
	Active site	209	1	Proton donor By similarity
	Binding site	72	1	Substrate By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
Amino acid modifications
	Glycosylation	105	1	N-linked (GlcNAc...) Potential
	Glycosylation	175	1	N-linked (GlcNAc...) Potential
	Glycosylation	270	1	N-linked (GlcNAc...) Potential
	Glycosylation	370	1	N-linked (GlcNAc...) Potential
	Glycosylation	403	1	N-linked (GlcNAc...) Potential
	Glycosylation	422	1	N-linked (GlcNAc...) Potential
	Glycosylation	435	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 74		By similarity
	Disulfide bond	121 ↔ 151		By similarity
	Disulfide bond	221 ↔ 237		By similarity
	Disulfide bond	223 ↔ 230		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41946 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 064FA6F462DD12C7	FASTA	471	52,088
	10 20 30 40 50 60 MFAFYFLTAC TTLKGVFGVS PSYNGLGLTP QMGWDSWNTF ACDVSEQLLL DTADRISDLG 70 80 90 100 110 120 LKDMGYKYVI LDDCWSSGRD SDGFLVADKH KFPNGMGHVA DHLHNNSFLF GMYSSAGEYT 130 140 150 160 170 180 CAGYPGSLGR EEEDAQFFAN NRVDYLKYDN CYNKGQFGTP DVSYHRYKAM SDALNKTGRP 190 200 210 220 230 240 IFYSLCNWGQ DLTFYWGSGI ANSWRMSGDI TAEFTRPDSR CPCDGDEYDC KYAGFHCSIM 250 260 270 280 290 300 NILNKAAPMG QNAGVGGWND LDNLEVGVGN LTDDEEKAHF SMWAMVKSPL IIGADVNHLK 310 320 330 340 350 360 ASSYSIYSQA SVIAINQDPK GIPATRVWRY YVSDTDEYGQ GEIQMWSGPL DNGDQVVALL 370 380 390 400 410 420 NGGSVARPMN TTLEEIFFDS NLGSKELTST WDIYDLWANR VDNSTASAIL EQNKAATGIL 430 440 450 460 470 YNATEQSYKD GLSKNDTRLF GQKIGSLSPN AILNTTVPAH GIAFYRLRPS A

References

[1]	"Consideration of the evolution of the Saccharomyces cerevisiae MEL gene family on the basis of the nucleotide sequences of the genes and their flanking regions." Turakainen H., Kristo P., Korhola M. Yeast 10:1559-1568(1994) [PubMed: 7725791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z37511 Genomic DNA. Translation: CAA85740.1.
PIR	S50311.
3D structure databases
ProteinModelPortal	P41946.
ModBase	Search...
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
SGD	S000029664. MEL5.
Gene expression databases
Genevestigator	P41946.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR006215. Glyco_hydro_melibiase. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27. PR00748. MELIBIASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MEL5_YEASX

Accession

Primary (citable) accession number: P41946

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 21, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program