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P41939 (IDHC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] cytoplasmic

Short name=IDH
EC=1.1.1.42
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene names
Name:IDP2
Ordered Locus Names:YLR174W
ORF Names:L9470.12
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function in the production of NADPH for fatty acid and sterol synthesis.

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Enzyme regulation

By catabolite repression.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Post-translational modification

The N-terminus is blocked.

Miscellaneous

Present with 2620 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Isocitrate dehydrogenase [NADP] cytoplasmic
PRO_0000083587

Regions

Nucleotide binding75 – 773NADP By similarity
Nucleotide binding310 – 3156NADP By similarity
Region94 – 1007Substrate binding By similarity

Sites

Metal binding2521Magnesium or manganese By similarity
Metal binding2751Magnesium or manganese By similarity
Binding site771Substrate By similarity
Binding site821NADP By similarity
Binding site1091Substrate By similarity
Binding site1321Substrate By similarity
Binding site2601NADP By similarity
Binding site3281NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1391Critical for catalysis By similarity
Site2121Critical for catalysis By similarity

Experimental info

Sequence conflict24 – 274HLIR → SFNQ in AAA64516. Ref.1
Sequence conflict681G → R in AAA64516. Ref.1
Sequence conflict1851T → N in AAA64516. Ref.1
Sequence conflict232 – 2398ARSYKEKF → LEVIKRSL in AAA64516. Ref.1
Sequence conflict2671I → M in AAA64516. Ref.1
Sequence conflict306 – 3072EA → DR in AAA64516. Ref.1
Sequence conflict316 – 3238FRQHQQGK → LTDYDKGR in AAA64516. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P41939 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 83EB54CC56A29857

FASTA41246,562
        10         20         30         40         50         60 
MTKIKVANPI VEMDGDEQTR IIWHLIRDKL VLPYLDVDLK YYDLSVEYRD QTNDQVTVDS 

        70         80         90        100        110        120 
ATATLKYGVA VKCATITPDE ARVEEFHLKK MWKSPNGTIR NILGGTVFRE PIIIPRIPRL 

       130        140        150        160        170        180 
VPQWEKPIII GRHAFGDQYK ATDVIVPEEG ELRLVYKSKS GTHDVDLKVF DYPEHGGVAM 

       190        200        210        220        230        240 
MMYNTTDSIE GFAKASFELA IERKLPLYST TKNTILKKYD GKFKDVFEAM YARSYKEKFE 

       250        260        270        280        290        300 
SLGIWYEHRL IDDMVAQMLK SKGGYIIAMK NYDGDVESDI VAQGFGSLGL MTSVLITPDG 

       310        320        330        340        350        360 
KTFESEAAHG TVTRHFRQHQ QGKETSTNSI ASIFAWTRGI IQRGKLDNTP DVVKFGQILE 

       370        380        390        400        410 
SATVNTVQED GIMTKDLALI LGKSERSAYV TTEEFIDAVE SRLKKEFEAA AL 

« Hide

References

« Hide 'large scale' references
[1]"Isolation, characterization, and disruption of the yeast gene encoding cytosolic NADP-specific isocitrate dehydrogenase."
Loftus T.M., Hall L.V., Anderson S.L., McAlister-Henn L.
Biochemistry 33:9661-9667(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L26312 Genomic DNA. Translation: AAA64516.1.
U17246 Genomic DNA. Translation: AAB67464.1.
BK006945 Genomic DNA. Translation: DAA09494.1.
PIRS51419.
RefSeqNP_013275.1. NM_001182061.1.

3D structure databases

ProteinModelPortalP41939.
SMRP41939. Positions 2-408.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31445. 17 interactions.
DIPDIP-4252N.
IntActP41939. 1 interaction.
MINTMINT-545477.
STRING4932.YLR174W.

Proteomic databases

PaxDbP41939.
PeptideAtlasP41939.
PRIDEP41939.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR174W; YLR174W; YLR174W.
GeneID850871.
KEGGsce:YLR174W.

Organism-specific databases

CYGDYLR174w.
SGDS000004164. IDP2.

Phylogenomic databases

eggNOGCOG0538.
GeneTreeENSGT00390000012547.
HOGENOMHOG000019858.
KOK00031.
OMAIIWHLIR.
OrthoDBEOG7G4QQD.

Enzyme and pathway databases

BioCycMetaCyc:YLR174W-MONOMER.
YEAST:YLR174W-MONOMER.
SABIO-RKP41939.

Gene expression databases

GenevestigatorP41939.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967206.

Entry information

Entry nameIDHC_YEAST
AccessionPrimary (citable) accession number: P41939
Secondary accession number(s): D6VYH8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families