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Protein

Glutathione reductase

Gene

GLR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Maintains high levels of reduced glutathione in the cytosol.

Miscellaneous

The active site is a redox-active disulfide bond.
Present with 7600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei472Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 61FADBy similarity9

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: SGD
  • cellular response to oxidative stress Source: SGD
  • glutathione metabolic process Source: SGD
  • protein glutathionylation Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciYEAST:YPL091W-MONOMER
BRENDAi1.8.1.7 984
ReactomeiR-SCE-3299685 Detoxification of Reactive Oxygen Species
R-SCE-499943 Interconversion of nucleotide di- and triphosphates
R-SCE-5263617 Metabolism of ingested MeSeO2H into MeSeH
R-SCE-5628897 TP53 Regulates Metabolic Genes

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione reductase (EC:1.8.1.7)
Short name:
GR
Short name:
GRase
Gene namesi
Name:GLR1
Ordered Locus Names:YPL091W
ORF Names:LPG17W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL091W
SGDiS000006012 GLR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4119

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000679721 – 483Glutathione reductaseAdd BLAST483
Isoform 2 (identifier: P41921-2)
Initiator methionineiRemovedCombined sources

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Disulfide bondi61 ↔ 66Redox-activeBy similarity
Isoform 2 (identifier: P41921-2)
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

MaxQBiP41921
PaxDbiP41921
PRIDEiP41921

2D gel databases

COMPLUYEAST-2DPAGEiP41921

PTM databases

iPTMnetiP41921

Interactioni

Protein-protein interaction databases

BioGridi36090, 82 interactors
DIPiDIP-4020N
IntActiP41921, 3 interactors
MINTiP41921
STRINGi4932.YPL091W

Chemistry databases

BindingDBiP41921

Structurei

Secondary structure

1483
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 29Combined sources7
Helixi33 – 44Combined sources12
Beta strandi49 – 55Combined sources7
Helixi59 – 64Combined sources6
Helixi66 – 82Combined sources17
Turni83 – 89Combined sources7
Helixi98 – 100Combined sources3
Helixi105 – 129Combined sources25
Beta strandi132 – 141Combined sources10
Beta strandi147 – 154Combined sources8
Beta strandi157 – 166Combined sources10
Beta strandi170 – 172Combined sources3
Helixi181 – 183Combined sources3
Helixi187 – 192Combined sources6
Beta strandi198 – 203Combined sources6
Helixi207 – 218Combined sources12
Beta strandi222 – 226Combined sources5
Beta strandi228 – 232Combined sources5
Helixi238 – 251Combined sources14
Beta strandi254 – 256Combined sources3
Beta strandi261 – 266Combined sources6
Beta strandi273 – 277Combined sources5
Beta strandi282 – 291Combined sources10
Beta strandi295 – 297Combined sources3
Helixi303 – 306Combined sources4
Beta strandi329 – 331Combined sources3
Helixi333 – 335Combined sources3
Helixi342 – 357Combined sources16
Helixi360 – 362Combined sources3
Beta strandi374 – 376Combined sources3
Beta strandi382 – 386Combined sources5
Helixi389 – 396Combined sources8
Helixi398 – 400Combined sources3
Beta strandi401 – 408Combined sources8
Helixi411 – 415Combined sources5
Beta strandi422 – 429Combined sources8
Turni430 – 433Combined sources4
Beta strandi434 – 442Combined sources9
Helixi445 – 457Combined sources13
Helixi462 – 466Combined sources5
Helixi476 – 480Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HQMX-ray2.40A/B17-483[»]
ProteinModelPortaliP41921
SMRiP41921
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41921

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

GeneTreeiENSGT00390000007578
HOGENOMiHOG000276712
InParanoidiP41921
KOiK00383
OMAiGLECACF
OrthoDBiEOG092C2ILO

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
IPR006322 Glutathione_Rdtase_euk/bac
IPR001100 Pyr_nuc-diS_OxRdtase
IPR004099 Pyr_nucl-diS_OxRdtase_dimer
IPR012999 Pyr_OxRdtase_I_AS
PANTHERiPTHR42737 PTHR42737, 1 hit
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF02852 Pyr_redox_dim, 1 hit
PIRSFiPIRSF000350 Mercury_reductase_MerA, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit
TIGRFAMsiTIGR01421 gluta_reduc_1, 1 hit
PROSITEiView protein in PROSITE
PS00076 PYRIDINE_REDOX_1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P41921-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSATKQTFR SLQIRTMSTN TKHYDYLVIG GGSGGVASAR RAASYGAKTL
60 70 80 90 100
LVEAKALGGT CVNVGCVPKK VMWYASDLAT RVSHANEYGL YQNLPLDKEH
110 120 130 140 150
LTFNWPEFKQ KRDAYVHRLN GIYQKNLEKE KVDVVFGWAR FNKDGNVEVQ
160 170 180 190 200
KRDNTTEVYS ANHILVATGG KAIFPENIPG FELGTDSDGF FRLEEQPKKV
210 220 230 240 250
VVVGAGYIGI ELAGVFHGLG SETHLVIRGE TVLRKFDECI QNTITDHYVK
260 270 280 290 300
EGINVHKLSK IVKVEKNVET DKLKIHMNDS KSIDDVDELI WTIGRKSHLG
310 320 330 340 350
MGSENVGIKL NSHDQIIADE YQNTNVPNIY SLGDVVGKVE LTPVAIAAGR
360 370 380 390 400
KLSNRLFGPE KFRNDKLDYE NVPSVIFSHP EAGSIGISEK EAIEKYGKEN
410 420 430 440 450
IKVYNSKFTA MYYAMLSEKS PTRYKIVCAG PNEKVVGLHI VGDSSAEILQ
460 470 480
GFGVAIKMGA TKADFDNCVA IHPTSAEELV TMR
Length:483
Mass (Da):53,441
Last modified:October 1, 1996 - v2
Checksum:i203BBB770120504A
GO
Isoform 2 (identifier: P41921-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: Missing.

Show »
Length:467
Mass (Da):51,578
Checksum:i0E4C84151AF31609
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti315Q → E in BAA07109 (Ref. 4) Curated1
Sequence conflicti428C → G in BAA07109 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0581241 – 16Missing in isoform 2. CuratedAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35342 Genomic DNA Translation: AAA92575.1
U43281 Genomic DNA Translation: AAB68208.1
D37871 mRNA Translation: BAA07109.1
BK006949 Genomic DNA Translation: DAA11342.1
PIRiS61975
RefSeqiNP_015234.1, NM_001183905.1 [P41921-1]

Genome annotation databases

EnsemblFungiiYPL091W; YPL091W; YPL091W [P41921-1]
GeneIDi856014
KEGGisce:YPL091W

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiGSHR_YEAST
AccessioniPrimary (citable) accession number: P41921
Secondary accession number(s): D6W3S6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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