P41920 (YRB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ran-specific GTPase-activating protein 1

Alternative name(s):
Chromosome stability protein 20
Perinuclear array-localized protein
Ran-binding protein 1
Short name=RANBP1

Gene names

Name:	YRB1
Synonyms:	CST20, HTN1, SFO1
Ordered Locus Names:	YDR002W
ORF Names:	YD8119.08

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important for the export of protein containing nuclear export signal (NES) out of the nucleus. Stimulates the GTPase activity of GSP1 and GSP2.
Subunit structure	Interacts with GSP1 and PRP20.
Subcellular location	Cytoplasm. Nucleus. Note: Shuttles between the nucleus and cytoplasm.
Sequence similarities	Belongs to the RANBP1 family. Contains 1 RanBD1 domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from mutant phenotype PubMed 10991951. Source: SGD RNA export from nucleus Inferred from mutant phenotype Ref.7. Source: SGD positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC protein import into nucleus Inferred from mutant phenotype Ref.7. Source: SGD ubiquitin-dependent protein catabolic process Inferred from mutant phenotype PubMed 10991951. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.8. Source: SGD nucleus Inferred from direct assay Ref.8. Source: SGD
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW Ran GTPase binding Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 201

201

Ran-specific GTPase-activating protein 1

PRO_0000213670

Regions

Domain

64 – 200

137

RanBD1

Amino acid modifications

Modified residue

23

1

Phosphoserine Ref.12

Modified residue

60

1

Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12

Secondary structure

1

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201

Helix Strand Turn

Details...

Beta strand

83 – 97

15

Turn

98 – 101

4

Beta strand

102 – 116

15

Turn

117 – 119

3

Beta strand

122 – 127

6

Turn

129 – 131

3

Beta strand

134 – 139

6

Beta strand

153 – 163

11

Beta strand

165 – 168

4

Beta strand

170 – 180

11

Helix

181 – 199

19

Sequences

	Sequence		Length	Mass (Da)
	P41920 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 46C3766B0906A75B	FASTA	201	22,953
	10 20 30 40 50 60 MSSEDKKPVV DKKEEAAPKP PSSAVFSMFG GKKAEKPETK KDEEDTKEET KKEGDDAPES 70 80 90 100 110 120 PDIHFEPVVH LEKVDVKTME EDEEVLYKVR AKLFRFDADA KEWKERGTGD CKFLKNKKTN 130 140 150 160 170 180 KVRILMRRDK TLKICANHII APEYTLKPNV GSDRSWVYAC TADIAEGEAE AFTFAIRFGS 190 200 KENADKFKEE FEKAQEINKK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast homologue of mammalian Ran binding protein 1." Butler G., Wolfe K.H. Biochim. Biophys. Acta 1219:711-712(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H9.
[2]	"Molecular analysis of the neutral trehalase gene from Saccharomyces cerevisiae." Kopp M., Mueller H., Holzer H. J. Biol. Chem. 268:4766-4774(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Ran-binding protein-1 is an essential component of the Ran/RCC1 molecular switch system in budding yeast." Ouspenski I.I., Mueller U.W., Matynia A., Sazer S., Elledge S.J., Brinkley B.R. J. Biol. Chem. 270:1975-1978(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[4]	Trueheart J., Richards S., Macara I.G., Thorner J. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Mutants in a yeast Ran binding protein are defective in nuclear transport." Schlenstedt G., Wong D.H., Koepp D.M., Silver P.A. EMBO J. 14:5367-5378(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Yeast Ran-binding protein 1 (Yrb1) shuttles between the nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway." Kunzler M., Gerstberger T., Stutz F., Bischoff F.R., Hurt E. Mol. Cell. Biol. 20:4295-4308(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND SER-60, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z33503 Genomic DNA. Translation: CAA83911.1.
X65925 Genomic DNA. No translation available.
L38489 Genomic DNA. Translation: AAA57276.1.
Z48008 Genomic DNA. Translation: CAA88062.1.
BK006938 Genomic DNA. Translation: DAA11849.1.

PIR

S50219.

RefSeq

NP_010285.1. NM_001180310.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3M1I	X-ray	2.00	B	11-201	[»]
4GMX	X-ray	2.10	B	62-201	[»]
4GPT	X-ray	2.22	B	62-201	[»]
4HAT	X-ray	1.78	B	62-201	[»]
4HAU	X-ray	2.00	B	62-201	[»]
4HAV	X-ray	2.00	B	62-201	[»]
4HAW	X-ray	1.90	B	62-201	[»]
4HAX	X-ray	2.28	B	62-201	[»]
4HAY	X-ray	2.30	B	62-201	[»]
4HAZ	X-ray	1.90	B	62-201	[»]
4HB0	X-ray	2.20	B	62-201	[»]
4HB2	X-ray	1.80	B	62-201	[»]
4HB3	X-ray	2.80	B	62-201	[»]
4HB4	X-ray	2.05	B	62-201	[»]

ProteinModelPortal

P41920.

SMR

P41920. Positions 71-201.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-838N.

IntAct

P41920. 5 interactions.

MINT

MINT-398068.

STRING

4932.YDR002W.

Proteomic databases

PaxDb

P41920.

PeptideAtlas

P41920.

PRIDE

P41920.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDR002W; YDR002W; YDR002W.

GeneID

851565.

KEGG

sce:YDR002W.

Organism-specific databases

CYGD

YDR002w.

SGD

S000002409. YRB1.

Phylogenomic databases

eggNOG

COG5171.

GeneTree

ENSGT00690000102083.

HOGENOM

HOG000176323.

KO

K15306.

OMA

HEDRDTT.

OrthoDB

EOG496327.

Gene expression databases

Genevestigator

P41920.

GermOnline

YDR002W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.30.29.30. 1 hit.

InterPro

IPR011993. PH_like_dom.
IPR000156. Ran_bind_dom.
[Graphical view]

Pfam

PF00638. Ran_BP1. 1 hit.
[Graphical view]

SMART

SM00160. RanBD. 1 hit.
[Graphical view]

PROSITE

PS50196. RANBD1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P41920.

NextBio

969007.

Entry information

Entry name

YRB1_YEAST

Accession

Primary (citable) accession number: P41920
Secondary accession number(s): D6VRY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program