P41917 (RAN2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP-binding nuclear protein Ran-2

Alternative name(s):
Ras-related nuclear protein 2

Gene names

Name:	RAN2
Ordered Locus Names:	At5g20020
ORF Names:	F28I16.170

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle By similarity.
Subunit structure	Interacts with PhrIP1, RanBP1a and RanBP1b. Ref.1 Ref.8
Subcellular location	Nucleus. Nucleus envelope. Note: Localized in the perinuclear region with the highest concentration at the nuclear envelope at the interphase. Ref.8
Sequence similarities	Belongs to the small GTPase superfamily. Ran family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Nucleus
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into nucleus Traceable author statement Ref.1. Source: TAIR response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay PubMed 15496452. Source: TAIR
Molecular_function	GTP binding Inferred from sequence or structural similarity Ref.1. Source: TAIR GTPase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	GTP-binding nuclear protein Ran-2		PRO_0000208718
Regions
	Nucleotide binding	20 – 27	8	GTP By similarity
	Nucleotide binding	68 – 72	5	GTP By similarity
	Nucleotide binding	125 – 128	4	GTP By similarity
Experimental info
	Sequence conflict	198 – 199	2	QQ → PK Ref.1
	Sequence conflict	198 – 199	2	QQ → PK Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P41917 [UniParc]. Last modified June 6, 2002. Version 3. Checksum: 349390AC56E05AFB	FASTA	221	25,062
	10 20 30 40 50 60 MALPNQQTVD YPSFKLVIVG DGGTGKTTFV KRHLTGEFEK KYEPTIGVEV HPLDFFTNCG 70 80 90 100 110 120 KIRFYCWDTA GQEKFGGLRD GYYIHGQCAI IMFDVTARLT YKNVPTWHRD LCRVCENIPI 130 140 150 160 170 180 VLCGNKVDVK NRQVKAKQVT FHRKKNLQYY EISAKSNYNF EKPFLYLARK LAGDQNLHFV 190 200 210 220 ESPALAPPEV HLDIAAQQQN EADLAAAAAQ PLPDDDDDAF E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis." Haizel T., Merkle T., Pay A., Fejes E., Nagy F. Plant J. 11:93-103(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RANBP1A AND RANBP1B.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Phenotype of the fission yeast cell cycle regulatory mutant pim1-46 is suppressed by a tobacco cDNA encoding a small, Ran-like GTP-binding protein." Merkle T., Haizel T., Matsumoto T., Harter K., Dallmann G., Nagy F. Plant J. 6:555-565(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-221.
[7]	"Analysis of the small GTPase gene superfamily of Arabidopsis." Vernoud V., Horton A.C., Yang Z., Nielsen E. Plant Physiol. 131:1191-1208(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Perinuclear and nuclear envelope localizations of Arabidopsis Ran proteins." Ma L., Hong Z., Zhang Z. Plant Cell Rep. 26:1373-1382(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PHRIP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97380 mRNA. Translation: CAA66048.1. AF296836 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED92780.1. AF370337 mRNA. Translation: AAK44152.1. AY062997 mRNA. Translation: AAL34171.1. BT000424 mRNA. Translation: AAN17401.1. BT000659 mRNA. Translation: AAN31806.1. BT006264 mRNA. Translation: AAP13372.1. AK226813 mRNA. Translation: BAE98909.1. L16790 mRNA. Translation: AAA32852.1.
IPI	IPI00535542.
RefSeq	NP_197502.1. NM_122009.3.
UniGene	At.177.
3D structure databases
ProteinModelPortal	P41917.
SMR	P41917. Positions 14-199.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41917. 2 interactions.
Proteomic databases
PaxDb	P41917.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G20020.1; AT5G20020.1; AT5G20020.
GeneID	832124.
KEGG	ath:AT5G20020.
Organism-specific databases
TAIR	At5g20020.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000216664.
InParanoid	P41917.
KO	K07936.
OMA	QIDMAMQ.
PhylomeDB	P41917.
ProtClustDB	PLN03071.
Gene expression databases
Genevestigator	P41917.
GermOnline	AT5G20020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002041. Ran_GTPase. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00627. GTPRANTC4.
SMART	SM00176. RAN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51418. RAN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RAN2_ARATH

Accession

Primary (citable) accession number: P41917
Secondary accession number(s): Q0WVD9, Q94K33

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 6, 2002
Last modified:	May 1, 2013
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program