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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial

Gene

GPD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the production of glycerol under anaerobic growth conditions. Glycerol production serves as a redox sink by consuming the excess cytosolic NADH during anaerobic metabolism.1 Publication

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei178 – 1781NADBy similarity
Binding sitei201 – 2011NAD; via amide nitrogenBy similarity
Binding sitei201 – 2011SubstrateBy similarity
Binding sitei234 – 2341NAD; via amide nitrogenBy similarity
Active sitei294 – 2941Proton acceptorBy similarity
Binding sitei359 – 3591NADBy similarity
Binding sitei388 – 3881NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 956NADBy similarity

GO - Molecular functioni

  • glycerol-3-phosphate dehydrogenase [NAD+] activity Source: SGD
  • NAD binding Source: InterPro

GO - Biological processi

  • glycerol-3-phosphate catabolic process Source: InterPro
  • glycerol metabolic process Source: SGD
  • NADH oxidation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YOL059W-MONOMER.
ReactomeiR-SCE-1483166. Synthesis of PA.
R-SCE-75109. Triglyceride Biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial (EC:1.1.1.8)
Gene namesi
Name:GPD2
Synonyms:GPD3
Ordered Locus Names:YOL059W
ORF Names:O1222
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL059W.
SGDiS000005420. GPD2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • glycerol-3-phosphate dehydrogenase complex Source: InterPro
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1616MitochondrionSequence analysisAdd
BLAST
Chaini17 – 440424Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrialPRO_0000043410Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701PhosphoserineCombined sources
Modified residuei72 – 721PhosphoserineCombined sources
Modified residuei75 – 751PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41911.

PTM databases

iPTMnetiP41911.

Expressioni

Inductioni

By anaerobic growth conditions and other conditions leading to accumulation of cytosolic NADH.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi34342. 58 interactions.
DIPiDIP-1348N.
IntActiP41911. 5 interactions.
MINTiMINT-411876.

Structurei

3D structure databases

ProteinModelPortaliP41911.
SMRiP41911. Positions 83-434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni359 – 3602Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000003114.
HOGENOMiHOG000246855.
InParanoidiP41911.
KOiK00006.
OMAiGDLCVTC.
OrthoDBiEOG092C3ZQP.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41911-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAVRRLTRY TFLKRTHPVL YTRRAYKILP SRSTFLRRSL LQTQLHSKMT
60 70 80 90 100
AHTNIKQHKH CHEDHPIRRS DSAVSIVHLK RAPFKVTVIG SGNWGTTIAK
110 120 130 140 150
VIAENTELHS HIFEPEVRMW VFDEKIGDEN LTDIINTRHQ NVKYLPNIDL
160 170 180 190 200
PHNLVADPDL LHSIKGADIL VFNIPHQFLP NIVKQLQGHV APHVRAISCL
210 220 230 240 250
KGFELGSKGV QLLSSYVTDE LGIQCGALSG ANLAPEVAKE HWSETTVAYQ
260 270 280 290 300
LPKDYQGDGK DVDHKILKLL FHRPYFHVNV IDDVAGISIA GALKNVVALA
310 320 330 340 350
CGFVEGMGWG NNASAAIQRL GLGEIIKFGR MFFPESKVET YYQESAGVAD
360 370 380 390 400
LITTCSGGRN VKVATYMAKT GKSALEAEKE LLNGQSAQGI ITCREVHEWL
410 420 430 440
QTCELTQEFP LFEAVYQIVY NNVRMEDLPE MIEELDIDDE
Length:440
Mass (Da):49,422
Last modified:October 1, 1996 - v2
Checksum:iFA6C07034D3EC720
GO

Sequence cautioni

The sequence CAA84532 differs from that shown. Reason: Frameshift at position 411. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35169 Genomic DNA. Translation: CAA84532.1. Sequence problems.
X91067 Genomic DNA. Translation: CAA62526.1.
Z74801 Genomic DNA. Translation: CAA99068.1.
AY558560 Genomic DNA. Translation: AAS56886.1.
BK006948 Genomic DNA. Translation: DAA10724.1.
PIRiS61719.
RefSeqiNP_014582.1. NM_001183314.1.

Genome annotation databases

EnsemblFungiiYOL059W; YOL059W; YOL059W.
GeneIDi854095.
KEGGisce:YOL059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35169 Genomic DNA. Translation: CAA84532.1. Sequence problems.
X91067 Genomic DNA. Translation: CAA62526.1.
Z74801 Genomic DNA. Translation: CAA99068.1.
AY558560 Genomic DNA. Translation: AAS56886.1.
BK006948 Genomic DNA. Translation: DAA10724.1.
PIRiS61719.
RefSeqiNP_014582.1. NM_001183314.1.

3D structure databases

ProteinModelPortaliP41911.
SMRiP41911. Positions 83-434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34342. 58 interactions.
DIPiDIP-1348N.
IntActiP41911. 5 interactions.
MINTiMINT-411876.

PTM databases

iPTMnetiP41911.

Proteomic databases

MaxQBiP41911.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL059W; YOL059W; YOL059W.
GeneIDi854095.
KEGGisce:YOL059W.

Organism-specific databases

EuPathDBiFungiDB:YOL059W.
SGDiS000005420. GPD2.

Phylogenomic databases

GeneTreeiENSGT00390000003114.
HOGENOMiHOG000246855.
InParanoidiP41911.
KOiK00006.
OMAiGDLCVTC.
OrthoDBiEOG092C3ZQP.

Enzyme and pathway databases

BioCyciYEAST:YOL059W-MONOMER.
ReactomeiR-SCE-1483166. Synthesis of PA.
R-SCE-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiP41911.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPD2_YEAST
AccessioniPrimary (citable) accession number: P41911
Secondary accession number(s): D6W208, P50905
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8966 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-49 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.