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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial

Gene

GPD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the production of glycerol under anaerobic growth conditions. Glycerol production serves as a redox sink by consuming the excess cytosolic NADH during anaerobic metabolism.1 Publication

Miscellaneous

Present with 8966 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-49 is the initiator.Curated

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei178NADBy similarity1
Binding sitei201NAD; via amide nitrogenBy similarity1
Binding sitei201SubstrateBy similarity1
Binding sitei234NAD; via amide nitrogenBy similarity1
Active sitei294Proton acceptorBy similarity1
Binding sitei359NADBy similarity1
Binding sitei388NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 95NADBy similarity6

GO - Molecular functioni

  • glycerol-3-phosphate dehydrogenase [NAD+] activity Source: SGD
  • NAD binding Source: InterPro
  • protein homodimerization activity Source: InterPro

GO - Biological processi

  • glycerol-3-phosphate catabolic process Source: InterPro
  • glycerol metabolic process Source: SGD
  • NADH oxidation Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YOL059W-MONOMER
ReactomeiR-SCE-1483166 Synthesis of PA

Protein family/group databases

MoonProtiP41911

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial (EC:1.1.1.8)
Gene namesi
Name:GPD2
Synonyms:GPD3
Ordered Locus Names:YOL059W
ORF Names:O1222
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL059W
SGDiS000005420 GPD2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 16MitochondrionSequence analysisAdd BLAST16
ChainiPRO_000004341017 – 440Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrialAdd BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphoserineCombined sources1
Modified residuei72PhosphoserineCombined sources1
Modified residuei75PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41911
PaxDbiP41911
PRIDEiP41911

PTM databases

iPTMnetiP41911

Expressioni

Inductioni

By anaerobic growth conditions and other conditions leading to accumulation of cytosolic NADH.2 Publications

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34342, 118 interactors
DIPiDIP-1348N
IntActiP41911, 7 interactors
MINTiP41911
STRINGi4932.YOL059W

Structurei

3D structure databases

ProteinModelPortaliP41911
SMRiP41911
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni359 – 360Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000003114
HOGENOMiHOG000246855
InParanoidiP41911
KOiK00006
OMAiPHQFIHK
OrthoDBiEOG092C3ZQP

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006168 G3P_DH_NAD-dep
IPR006109 G3P_DH_NAD-dep_C
IPR017751 G3P_DH_NAD-dep_euk
IPR011128 G3P_DH_NAD-dep_N
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF07479 NAD_Gly3P_dh_C, 1 hit
PF01210 NAD_Gly3P_dh_N, 1 hit
PRINTSiPR00077 GPDHDRGNASE
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR03376 glycerol3P_DH, 1 hit
PROSITEiView protein in PROSITE
PS00957 NAD_G3PDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41911-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAVRRLTRY TFLKRTHPVL YTRRAYKILP SRSTFLRRSL LQTQLHSKMT
60 70 80 90 100
AHTNIKQHKH CHEDHPIRRS DSAVSIVHLK RAPFKVTVIG SGNWGTTIAK
110 120 130 140 150
VIAENTELHS HIFEPEVRMW VFDEKIGDEN LTDIINTRHQ NVKYLPNIDL
160 170 180 190 200
PHNLVADPDL LHSIKGADIL VFNIPHQFLP NIVKQLQGHV APHVRAISCL
210 220 230 240 250
KGFELGSKGV QLLSSYVTDE LGIQCGALSG ANLAPEVAKE HWSETTVAYQ
260 270 280 290 300
LPKDYQGDGK DVDHKILKLL FHRPYFHVNV IDDVAGISIA GALKNVVALA
310 320 330 340 350
CGFVEGMGWG NNASAAIQRL GLGEIIKFGR MFFPESKVET YYQESAGVAD
360 370 380 390 400
LITTCSGGRN VKVATYMAKT GKSALEAEKE LLNGQSAQGI ITCREVHEWL
410 420 430 440
QTCELTQEFP LFEAVYQIVY NNVRMEDLPE MIEELDIDDE
Length:440
Mass (Da):49,422
Last modified:October 1, 1996 - v2
Checksum:iFA6C07034D3EC720
GO

Sequence cautioni

The sequence CAA84532 differs from that shown. Reason: Frameshift at position 411.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35169 Genomic DNA Translation: CAA84532.1 Sequence problems.
X91067 Genomic DNA Translation: CAA62526.1
Z74801 Genomic DNA Translation: CAA99068.1
AY558560 Genomic DNA Translation: AAS56886.1
BK006948 Genomic DNA Translation: DAA10724.1
PIRiS61719
RefSeqiNP_014582.1, NM_001183314.1

Genome annotation databases

EnsemblFungiiYOL059W; YOL059W; YOL059W
GeneIDi854095
KEGGisce:YOL059W

Similar proteinsi

Entry informationi

Entry nameiGPD2_YEAST
AccessioniPrimary (citable) accession number: P41911
Secondary accession number(s): D6W208, P50905
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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