Reviewed, UniProtKB/Swiss-Prot P41911 (GPD2_YEAST)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+] 2, mitochondrial
    EC=1.1.1.8

Gene names

Name:	GPD2
Synonyms:	GPD3
Ordered Locus Names:	YOL059W
ORF Names:	O1222

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the production of glycerol under anaerobic growth conditions. Glycerol production serves as a redox sink by consuming the excess cytosolic NADH during anaerobic metabolism. Ref.5
Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Subcellular location	Cytoplasm. Mitochondrion Ref.8 Ref.10 Ref.11.
Induction	By anaerobic growth conditions and other conditions leading to accumulation of cytosolic NADH. Ref.11 Ref.6
Miscellaneous	Present with 8966 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
Caution	It is uncertain whether Met-1 or Met-49 is the initiator.
Sequence caution	The sequence CAA84532.1 differs from that shown. Reason: Frameshift at position 411.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADH oxidation Ref.6 Inferred from mutant phenotype. Source: SGD glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Traceable author statement. Source: SGD glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro mitochondrion Ref.10 Inferred from direct assay. Source: SGD
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Ref.6 Inferred from mutant phenotype. Source: SGD protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IPP1	P00817	1	EBI-7791,EBI-9338
RNR1	P21524	1	EBI-7791,EBI-15234
RNR2	P09938	1	EBI-7791,EBI-15240
VMA2	P16140	1	EBI-7791,EBI-20254

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Mitochondrion Potential
	Chain	17 – 440	424	Glycerol-3-phosphate dehydrogenase [NAD+] 2, mitochondrial		PRO_0000043410
Regions
	Nucleotide binding	90 – 95	6	NAD By similarity
	Region	359 – 360	2	Substrate binding By similarity
Sites
	Active site	294	1	Proton acceptor By similarity
	Binding site	178	1	NAD By similarity
	Binding site	201	1	NAD; via amide nitrogen By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	234	1	NAD; via amide nitrogen By similarity
	Binding site	359	1	NAD By similarity
	Binding site	388	1	NAD By similarity
Amino acid modifications
	Modified residue	70	1	Phosphoserine Ref.13 Ref.15
	Modified residue	72	1	Phosphoserine Ref.13 Ref.15 Ref.7 Ref.12 Ref.14
	Modified residue	75	1	Phosphoserine Ref.13 Ref.15 Ref.7 Ref.12 Ref.14

Sequences

	Sequence		Length	Mass (Da)
	P41911-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: FA6C07034D3EC720	FASTA	440	49,422
	10 20 30 40 50 60 MLAVRRLTRY TFLKRTHPVL YTRRAYKILP SRSTFLRRSL LQTQLHSKMT AHTNIKQHKH 70 80 90 100 110 120 CHEDHPIRRS DSAVSIVHLK RAPFKVTVIG SGNWGTTIAK VIAENTELHS HIFEPEVRMW 130 140 150 160 170 180 VFDEKIGDEN LTDIINTRHQ NVKYLPNIDL PHNLVADPDL LHSIKGADIL VFNIPHQFLP 190 200 210 220 230 240 NIVKQLQGHV APHVRAISCL KGFELGSKGV QLLSSYVTDE LGIQCGALSG ANLAPEVAKE 250 260 270 280 290 300 HWSETTVAYQ LPKDYQGDGK DVDHKILKLL FHRPYFHVNV IDDVAGISIA GALKNVVALA 310 320 330 340 350 360 CGFVEGMGWG NNASAAIQRL GLGEIIKFGR MFFPESKVET YYQESAGVAD LITTCSGGRN 370 380 390 400 410 420 VKVATYMAKT GKSALEAEKE LLNGQSAQGI ITCREVHEWL QTCELTQEFP LFEAVYQIVY 430 440 NNVRMEDLPE MIEELDIDDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of GPD2, a second gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) in Saccharomyces cerevisiae, and its comparison with GPD1." Eriksson P., Andre L., Ansell R., Blomberg A., Adler L. Mol. Microbiol. 17:95-107(1995) [PubMed: 7476212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of a 26 kb region on the left arm of yeast chromosome XV." Mannhaupt G., Vetter I., Schwarzlose C., Mitzel S., Feldmann H. Yeast 12:67-76(1996) [PubMed: 8789261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 90843 / S288c / FY73.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 90843 / S288c / FY73.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Physiological response to anaerobicity of glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae." Bjoerkqvist S., Ansell R., Adler L., Liden G. Appl. Environ. Microbiol. 63:128-132(1997) [PubMed: 8979347] [Abstract] Cited for: FUNCTION.
[6]	"The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation." Ansell R., Granath K., Hohmann S., Thevelein J.M., Adler L. EMBO J. 16:2179-2187(1997) [PubMed: 9171333] [Abstract] Cited for: INDUCTION.
[7]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72 AND SER-75, MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]	"Distinct intracellular localization of Gpd1p and Gpd2p, the two yeast isoforms of NAD+-dependent glycerol-3-phosphate dehydrogenase, explains their different contributions to redox-driven glycerol production." Valadi A., Granath K., Gustafsson L., Adler L. J. Biol. Chem. 279:39677-39685(2004) [PubMed: 15210723] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION.
[12]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72 AND SER-75, MASS SPECTROMETRY.
[13]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70; SER-72 AND SER-75, MASS SPECTROMETRY.
[14]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72 AND SER-75, MASS SPECTROMETRY.
[15]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70; SER-72 AND SER-75, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35169 Genomic DNA. Translation: CAA84532.1. Sequence problems. X91067 Genomic DNA. Translation: CAA62526.1. Z74801 Genomic DNA. Translation: CAA99068.1. AY558560 Genomic DNA. Translation: AAS56886.1.
PIR	S61719.
RefSeq	NP_014582.1.
3D structure databases
SMR	P41911. Positions 82-431.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1348N.
IntAct	P41911. 12 interactions.
STRING	P41911.
Proteomic databases
PeptideAtlas	P41911.
Genome annotation databases
Ensembl	YOL059W; YOL059W; YOL059W; Saccharomyces cerevisiae. [Genome view]
GeneID	854095.
KEGG	sce:YOL059W.
NMPDR	fig|4932.3.peg.5674.
Organism-specific databases
CYGD	YOL059w.
SGD	S000005420. GPD2.
Phylogenomic databases
eggNOG	fuNOG04977.
HOGENOM	HBG586392.
OMA	THESAGV.
OrthoDB	EOG9J9PGK.
PhylomeDB	P41911.
Enzyme and pathway databases
BRENDA	1.1.1.8. 250.
Gene expression databases
ArrayExpress	P41911.
Genevestigator	P41911.
GermOnline	YOL059W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PRINTS	PR00077. GPDHDRGNASE.
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975758.

Entry information

Entry name

GPD2_YEAST

Accession

Primary (citable) accession number: P41911
Secondary accession number(s): P50905

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)