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Protein

Peroxisomal long-chain fatty acid import protein 2

Gene

PXA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the import of activated long-chain fatty acids from the cytosol to the peroxisomal matrix.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi645 – 6528ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: SGD
  • ATP binding Source: UniProtKB-KW
  • peroxisomal fatty-acyl-CoA transporter activity Source: UniProtKB

GO - Biological processi

  • fatty acid beta-oxidation Source: UniProtKB
  • fatty acid transport Source: SGD
  • fatty-acyl-CoA transport Source: UniProtKB
  • long-chain fatty acid catabolic process Source: UniProtKB
  • peroxisomal long-chain fatty acid import Source: UniProtKB
  • very long-chain fatty acid catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34044-MONOMER.
BRENDAi3.6.3.47. 984.
ReactomeiR-SCE-1369062. ABC transporters in lipid homeostasis.
R-SCE-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.
R-SCE-2046105. Linoleic acid (LA) metabolism.
R-SCE-2046106. alpha-linolenic acid (ALA) metabolism.
R-SCE-390247. Beta-oxidation of very long chain fatty acids.

Protein family/group databases

TCDBi3.A.1.203.6. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal long-chain fatty acid import protein 2
Alternative name(s):
Peroxisomal ABC transporter 1
Gene namesi
Name:PXA1
Synonyms:PAL1, PAT2, SSH2
Ordered Locus Names:YPL147W
ORF Names:LPI1W, P2607
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL147W.
SGDiS000006068. PXA1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei60 – 8021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei207 – 22721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei247 – 26721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei330 – 35021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei353 – 37321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei455 – 47521HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of peroxisomal membrane Source: SGD
  • peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 870870Peroxisomal long-chain fatty acid import protein 2PRO_0000093314Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence analysis
Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence analysis
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP41909.
PeptideAtlasiP41909.
PRIDEiP41909.

Interactioni

Subunit structurei

Forms a heterodimer with PXA2.

Binary interactionsi

WithEntry#Exp.IntActNotes
PXA2P342302EBI-14344,EBI-2464632

Protein-protein interaction databases

BioGridi36036. 81 interactions.
DIPiDIP-7809N.
IntActiP41909. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP41909.
SMRiP41909. Positions 607-823.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini210 – 501292ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini608 – 866259ABC transporterPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003950.
HOGENOMiHOG000206081.
InParanoidiP41909.
KOiK15628.
OMAiFNGIRLE.
OrthoDBiEOG7H4F37.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005283. FA_transporter.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06472. ABC_membrane_2. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00954. 3a01203. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTLAAPAK LKSLLLNLHT HCIGLHVNDV TPKVYFKLLI RHLLQISRSN
60 70 80 90 100
AAHPKLRRRA QILLVSLFLS GVTLFSGVTY STFKIILKCY KFYKFPWKRR
110 120 130 140 150
NRRPLIRRTR SQMQLDSGAR IMYIPEVELV DRQSPDDNKF MNATDKKKRK
160 170 180 190 200
RIFIPPKDND VYEHDKFLFK NVELERAKNS QLFYSKFLNQ MNVLSKILIP
210 220 230 240 250
TVFDKNFLLL TAQIFFLVMR TWLSLFVAKL DGQIVKNIIA GRGRSFLWDL
260 270 280 290 300
GCWFLIAVPA SYTNSAIKLL QRKLSLNFRV NLTRYIHDMY LDKRLTFYKL
310 320 330 340 350
IFDAKASNSV IKNIDNSITN DVAKFCDATC SVFANIAKPV IDLIFFSVYL
360 370 380 390 400
RDNLGTVGVA GIFVNYFITG FILRKYTPPL GKLAGERSAS DGDYYNYHLN
410 420 430 440 450
MINNSEEIAF YQGTAVERTK VKELYDVLME KMLLVDKVKF GYNMLEDYVL
460 470 480 490 500
KYTWSGLGYV FASIPIVMST LATGINSEEK NMKEFIVNKR LMLSLADAGS
510 520 530 540 550
RLMHSIKDIS QLTGYTNRIF TLLSVLHRVH SLNFNYGAVP SILSIRTEDA
560 570 580 590 600
SRNSNLLPTT DNSQDAIRGT IQRNFNGIRL ENIDVIIPSV RASEGIKLIN
610 620 630 640 650
KLTFQIPLHI DPITSKSNSI QDLSKANDIK LPFLQGSGSS LLILGPNGCG
660 670 680 690 700
KSSIQRIIAE IWPVYNKNGL LSIPSENNIF FIPQKPYFSR GGTLRDQIIY
710 720 730 740 750
PMSSDEFFDR GFRDKELVQI LVEVKLDYLL KRGVGLTYLD AIADWKDLLS
760 770 780 790 800
GGEKQRVNFA RIMFHKPLYV VLDEATNAIS VDMEDYLFNL LKRYRFNFIS
810 820 830 840 850
ISQRPTLIKY HEMLLEIGEN RDGKWQLQAV GTDEAITSID NEIEELERKL
860 870
ERVKGWEDER TKLREKLEII
Length:870
Mass (Da):100,025
Last modified:September 5, 2006 - v2
Checksum:i45B20F888802E00A
GO

Sequence cautioni

The sequence AAB68215.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAC49009.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17065 Genomic DNA. Translation: AAC49009.1. Different initiation.
L38491 Genomic DNA. Translation: AAC37480.1.
X96770 Genomic DNA. Translation: CAA65546.1.
U43703 Genomic DNA. Translation: AAB68215.1. Different initiation.
Z73503 Genomic DNA. Translation: CAA97852.1.
Z73502 Genomic DNA. Translation: CAA97851.1.
BK006949 Genomic DNA. Translation: DAA11288.1.
PIRiS65158.
RefSeqiNP_015178.1. NM_001183961.1.

Genome annotation databases

EnsemblFungiiYPL147W; YPL147W; YPL147W.
GeneIDi855956.
KEGGisce:YPL147W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17065 Genomic DNA. Translation: AAC49009.1. Different initiation.
L38491 Genomic DNA. Translation: AAC37480.1.
X96770 Genomic DNA. Translation: CAA65546.1.
U43703 Genomic DNA. Translation: AAB68215.1. Different initiation.
Z73503 Genomic DNA. Translation: CAA97852.1.
Z73502 Genomic DNA. Translation: CAA97851.1.
BK006949 Genomic DNA. Translation: DAA11288.1.
PIRiS65158.
RefSeqiNP_015178.1. NM_001183961.1.

3D structure databases

ProteinModelPortaliP41909.
SMRiP41909. Positions 607-823.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36036. 81 interactions.
DIPiDIP-7809N.
IntActiP41909. 2 interactions.

Protein family/group databases

TCDBi3.A.1.203.6. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBiP41909.
PeptideAtlasiP41909.
PRIDEiP41909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL147W; YPL147W; YPL147W.
GeneIDi855956.
KEGGisce:YPL147W.

Organism-specific databases

EuPathDBiFungiDB:YPL147W.
SGDiS000006068. PXA1.

Phylogenomic databases

GeneTreeiENSGT00390000003950.
HOGENOMiHOG000206081.
InParanoidiP41909.
KOiK15628.
OMAiFNGIRLE.
OrthoDBiEOG7H4F37.

Enzyme and pathway databases

BioCyciYEAST:G3O-34044-MONOMER.
BRENDAi3.6.3.47. 984.
ReactomeiR-SCE-1369062. ABC transporters in lipid homeostasis.
R-SCE-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.
R-SCE-2046105. Linoleic acid (LA) metabolism.
R-SCE-2046106. alpha-linolenic acid (ALA) metabolism.
R-SCE-390247. Beta-oxidation of very long chain fatty acids.

Miscellaneous databases

NextBioi980747.
PROiP41909.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005283. FA_transporter.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06472. ABC_membrane_2. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00954. 3a01203. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PXA1, a possible Saccharomyces cerevisiae ortholog of the human adrenoleukodystrophy gene."
    Shani N., Watkins P.A., Valle D.
    Proc. Natl. Acad. Sci. U.S.A. 92:6012-6016(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CH1305.
  2. "The PAL1 gene product is a peroxisomal ATP-binding cassette transporter in the yeast Saccharomyces cerevisiae."
    Swartzman E.E., Viswanathan M.N., Thorner J.
    J. Cell Biol. 132:549-563(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The ABC transporter proteins Pat1 and Pat2 are required for import of long-chain fatty acids into peroxisomes of Saccharomyces cerevisiae."
    Hettema E.H., van Roermund C.W.T., Distel B., van den Berg M., Vilela C., Rodrigues-Pousada C., Wanders R.J.A., Tabak H.F.
    EMBO J. 15:3813-3822(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPXA1_YEAST
AccessioniPrimary (citable) accession number: P41909
Secondary accession number(s): D6W3M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 5, 2006
Last modified: April 13, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.