Reviewed, UniProtKB/Swiss-Prot P41903 (PTE1_YEAST)

Names and origin

Protein names

Recommended name:
    Peroxisomal acyl-coenzyme A thioester hydrolase 1
    EC=3.1.2.2
Alternative name(s):
    Peroxisomal long-chain acyl-CoA thioesterase 1

Gene names

Name:	TES1
Synonyms:	PTE1
Ordered Locus Names:	YJR019C
ORF Names:	J1456

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.
Catalytic activity	Palmitoyl-CoA + H2O = CoA + palmitate.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the C/M/P thioester hydrolase family.

Ontologies

Keywords
Cellular component	Peroxisome
Molecular function	Hydrolase Serine esterase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	acyl-CoA metabolic process Inferred from electronic annotation. Source: InterPro fatty acid beta-oxidation Inferred from genetic interaction. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD peroxisome Ref.2 Inferred from direct assay. Source: SGD
Molecular function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW palmitoyl-CoA hydrolase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P43582	1	EBI-14154,EBI-22766
PRP40	P33203	1	EBI-14154,EBI-701
SSM4	P40318	1	EBI-14154,EBI-18208

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 349

349

Peroxisomal acyl-coenzyme A thioester hydrolase 1

PRO_0000202158

Regions

Motif

347 – 349

3

Microbody targeting signal Potential

Sites

Active site

259

1

Charge relay system By similarity

Active site

282

1

Charge relay system By similarity

Active site

333

1

Charge relay system By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

349

Helix Strand Turn

Details...

Beta strand

17 – 19

3

Beta strand

21 – 29

9

Helix

42 – 54

13

Beta strand

62 – 70

9

Beta strand

81 – 89

9

Beta strand

91 – 102

12

Beta strand

105 – 115

11

Sequences

	Sequence		Length	Mass (Da)
	P41903-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F1B5A51C9A46783E	FASTA	349	40,260
	10 20 30 40 50 60 MSASKMAMSN LEKILELVPL SPTSFVTKYL PAAPVGSKGT FGGTLVSQSL LASLHTVPLN 70 80 90 100 110 120 FFPTSLHSYF IKGGDPRTKI TYHVQNLRNG RNFIHKQVSA YQHDKLIFTS MILFAVQRSK 130 140 150 160 170 180 EHDSLQHWET IPGLQGKQPD PHRYEEATSL FQKEVLDPQK LSRYASLSDR FQDATSMSKY 190 200 210 220 230 240 VDAFQYGVME YQFPKDMFYS ARHTDELDYF VKVRPPITTV EHAGDESSLH KHHPYRIPKS 250 260 270 280 290 300 ITPENDARYN YVAFAYLSDS YLLLTIPYFH NLPLYCHSFS VSLDHTIYFH QLPHVNNWIY 310 320 330 340 LKISNPRSHW DKHLVQGKYF DTQSGRIMAS VSQEGYVVYG SERDIRAKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hani J., Stumpf G., Domdey H. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DH484.
[2]	"Identification of peroxisomal acyl-CoA thioesterases in yeast and humans." Jones J.M., Nau K., Geraghty M.T., Erdmann R., Gould S.J. J. Biol. Chem. 274:9216-9223(1999) [PubMed: 10092594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X85972 Genomic DNA. Translation: CAA59960.1.
X87611 Genomic DNA. Translation: CAA60943.1.
AF124265 Genomic DNA. Translation: AAD27617.1.
Z49519 Genomic DNA. Translation: CAA89543.1.

PIR

S52763.

RefSeq

NP_012553.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TBU	X-ray	2.20	A/B/C/D	1-118	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:4480N.

IntAct

P41903. 6 interactions.

STRING

P41903.

Proteomic databases

PeptideAtlas

P41903.

Genome annotation databases

Ensembl

YJR019C; YJR019C; YJR019C; Saccharomyces cerevisiae. [Genome view]

GeneID

853477.

GenomeReviews

Gene locus YJR019C in contig Y13136_GR.

KEGG

sce:YJR019C.

NMPDR

fig|4932.3.peg.3527.

Organism-specific databases

CYGD

YJR019c.

SGD

S000003780. TES1.

Phylogenomic databases

HOGENOM

P41903.

OMA

NIWIRAN.

Enzyme and pathway databases

BRENDA

3.1.2.2. 250.

Gene expression databases

ArrayExpress

P41903.

Genevestigator

P41903.

GermOnline

YJR019C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR003703. Acyl_CoA_thio.
[Graphical view]

PANTHER

PTHR11066. Acyl_CoA_thio. 1 hit.

Pfam

PF02551. Acyl_CoA_thio. 2 hits.
[Graphical view]

TIGRFAMs

TIGR00189. tesB. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

974083.

Entry information

Entry name

PTE1_YEAST

Accession

Primary (citable) accession number: P41903

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)