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Protein

Sporulation-regulated protein 3

Gene

SPR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Septins are GTPases involved in cytokinesis that assemble into filaments and form a ring at the cleavage site. May act by recruiting MYO1 and HOF1, a protein involved in septation, to the site of cleavage. Septins are also involved in cell morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei168GTP; via amide nitrogenBy similarity1
Binding sitei315GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi116 – 123GTPBy similarity8
Nucleotide bindingi247 – 255GTPBy similarity9

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • structural molecule activity Source: SGD

GO - Biological processi

  • ascospore formation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Sporulation

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30776-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation-regulated protein 3
Gene namesi
Name:SPR3
Ordered Locus Names:YGR059W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR059W.
SGDiS000003291. SPR3.

Subcellular locationi

  • Prospore membrane By similarity; Peripheral membrane protein By similarity
  • Bud neck By similarity

  • Note: Present at the bud neck during cell division. Probably interacts with phosphoinosides such as phosphatidylinositol 4-phosphate or phosphatidylinositol 5-phosphate (By similarity). Are localized to the leading edges of the membrane sacs that form near the spindle-pole bodies and gradually extend to engulf the nuclear lobes that contain the haploid chromosome sets, thus forming the spores.By similarity

GO - Cellular componenti

  • ascospore wall Source: SGD
  • cellular bud neck Source: UniProtKB-SubCell
  • prospore membrane Source: SGD
  • septin complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001735021 – 512Sporulation-regulated protein 3Add BLAST512

Proteomic databases

PRIDEiP41901.

Expressioni

Developmental stagei

Sporulation-specific.

Interactioni

Subunit structurei

Interacts with other septin proteins such as SPR28 to form a ring at the bud neck.1 Publication

Protein-protein interaction databases

BioGridi33304. 92 interactors.
DIPiDIP-3011N.
IntActiP41901. 2 interactors.
MINTiMINT-437888.

Structurei

3D structure databases

ProteinModelPortaliP41901.
SMRiP41901.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini106 – 365Septin-type GAdd BLAST260

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili376 – 406Sequence analysisAdd BLAST31
Coiled coili451 – 496Sequence analysisAdd BLAST46

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00860000134437.
InParanoidiP41901.
KOiK12773.
OMAiRSYIFQE.
OrthoDBiEOG092C3HL6.

Family and domain databases

CDDicd01850. CDC_Septin. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR030379. G_SEPTIN_dom.
IPR027417. P-loop_NTPase.
IPR016491. Septin.
[Graphical view]
PANTHERiPTHR18884. PTHR18884. 2 hits.
PfamiPF00735. Septin. 1 hit.
[Graphical view]
PIRSFiPIRSF006698. Septin. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51719. G_SEPTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41901-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSKGSRLST DCPVEFPKIV SGFAEEVKIR RQSSQGQYAV DSHPPKSPEL
60 70 80 90 100
KHRRQRSSSF VNGKCRNRDL PLLDNKKAQE INTNSHGQDI GIKNLPRQRE
110 120 130 140 150
LLNAKNGIDF TLMVAGQSGL GKTTFINSLF STSLIDDDIK ENKPIIRYKS
160 170 180 190 200
IVEGDGTHLN FNVIDTPGFG NNMDNAFTWR TMVNYIDEEI RSYIFQEEQP
210 220 230 240 250
DRTKMVDNRV HCCLYFLRPS NKGIDTLDVV TMKKLAKRVN LIPVIAKSDL
260 270 280 290 300
LTKEELKNFK TQVREIIRVQ DIPVCFFFGD EVLNATQDIF QKYPFSIIAS
310 320 330 340 350
NEYIFNEKGE KVKGRQYKWG AVDIENEKYC DFKILQKTIF DWNLIDLVES
360 370 380 390 400
TEDYYEKCRS EMLRTRLLKA RDCLTTKSVD ITEEQRKFLE EEMNFDEIEE
410 420 430 440 450
NKLKNYKCYE IINKTVMDKV ATEWDPEFIT RQLEAKKKFN ELSNREISKF
460 470 480 490 500
RDWKKSLFME QENFNQEIEQ LNHKLENLQL ECQDLEYKLL IGKSSNSHST
510
DSATLVNVHI KR
Length:512
Mass (Da):59,845
Last modified:November 1, 1995 - v1
Checksum:i1FC55B13E6A88F5D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26E → D in AAC13870 (PubMed:7590307).Curated1
Sequence conflicti250L → C in AAC13870 (PubMed:7590307).Curated1
Sequence conflicti263V → L in AAC13870 (PubMed:7590307).Curated1
Sequence conflicti317Y → H in AAC13870 (PubMed:7590307).Curated1
Sequence conflicti481E → G in AAC13870 (PubMed:7590307).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31767 Genomic DNA. Translation: AAC37482.1.
U24129 Genomic DNA. Translation: AAC13870.1.
Z72844 Genomic DNA. Translation: CAA97061.1.
BK006941 Genomic DNA. Translation: DAA08155.1.
PIRiS48524.
RefSeqiNP_011573.3. NM_001181188.3.

Genome annotation databases

EnsemblFungiiYGR059W; YGR059W; YGR059W.
GeneIDi852950.
KEGGisce:YGR059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31767 Genomic DNA. Translation: AAC37482.1.
U24129 Genomic DNA. Translation: AAC13870.1.
Z72844 Genomic DNA. Translation: CAA97061.1.
BK006941 Genomic DNA. Translation: DAA08155.1.
PIRiS48524.
RefSeqiNP_011573.3. NM_001181188.3.

3D structure databases

ProteinModelPortaliP41901.
SMRiP41901.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33304. 92 interactors.
DIPiDIP-3011N.
IntActiP41901. 2 interactors.
MINTiMINT-437888.

Proteomic databases

PRIDEiP41901.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR059W; YGR059W; YGR059W.
GeneIDi852950.
KEGGisce:YGR059W.

Organism-specific databases

EuPathDBiFungiDB:YGR059W.
SGDiS000003291. SPR3.

Phylogenomic databases

GeneTreeiENSGT00860000134437.
InParanoidiP41901.
KOiK12773.
OMAiRSYIFQE.
OrthoDBiEOG092C3HL6.

Enzyme and pathway databases

BioCyciYEAST:G3O-30776-MONOMER.

Miscellaneous databases

PROiP41901.

Family and domain databases

CDDicd01850. CDC_Septin. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR030379. G_SEPTIN_dom.
IPR027417. P-loop_NTPase.
IPR016491. Septin.
[Graphical view]
PANTHERiPTHR18884. PTHR18884. 2 hits.
PfamiPF00735. Septin. 1 hit.
[Graphical view]
PIRSFiPIRSF006698. Septin. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51719. G_SEPTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPR3_YEAST
AccessioniPrimary (citable) accession number: P41901
Secondary accession number(s): D6VUJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.