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Protein

Transcription initiation factor IIF subunit beta

Gene

TFG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TFG2 shows ATP-dependent DNA-helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

GO - Biological processi

  • promoter clearance from RNA polymerase II promoter Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: GOC
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • transcriptional start site selection at RNA polymerase II promoter Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
  • transcription initiation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30736-MONOMER.
ReactomeiREACT_273109. Formation of the Early Elongation Complex.
REACT_277632. mRNA Capping.
REACT_294700. mRNA Splicing - Minor Pathway.
REACT_296055. RNA Polymerase II Promoter Escape.
REACT_300180. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_310018. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_315180. RNA Polymerase II Pre-transcription Events.
REACT_317581. Processing of Capped Intron-Containing Pre-mRNA.
REACT_322294. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_345860. RNA Polymerase II Transcription Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIF subunit beta (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase TFG2
TFIIF medium subunit
TFIIF-beta
Transcription factor G 54 kDa subunit
Gene namesi
Name:TFG2
Ordered Locus Names:YGR005C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGR005c.
EuPathDBiFungiDB:YGR005C.
SGDiS000003237. TFG2.

Subcellular locationi

GO - Cellular componenti

  • transcription factor TFIIF complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Transcription initiation factor IIF subunit betaPRO_0000211240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281Phosphoserine1 Publication
Modified residuei34 – 341Phosphoserine1 Publication
Modified residuei56 – 561Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41896.
PaxDbiP41896.
PeptideAtlasiP41896.

Interactioni

Subunit structurei

TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.

Protein-protein interaction databases

BioGridi33249. 133 interactions.
DIPiDIP-1152N.
IntActiP41896. 21 interactions.
MINTiMINT-562451.
STRINGi4932.YGR005C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V1Nelectron microscopy7.80R1-400[»]
4V1Oelectron microscopy9.70R1-400[»]
ProteinModelPortaliP41896.
SMRiP41896. Positions 54-142, 293-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIF beta subunit family.Curated

Phylogenomic databases

eggNOGiCOG5090.
GeneTreeiENSGT00390000016051.
HOGENOMiHOG000000947.
InParanoidiP41896.
KOiK03139.
OMAiLKERTRQ.
OrthoDBiEOG7VB2RC.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003196. TFIIF_beta.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10445. PTHR10445. 1 hit.
PfamiPF02270. TFIIF_beta. 1 hit.
[Graphical view]
SUPFAMiSSF50916. SSF50916. 1 hit.

Sequencei

Sequence statusi: Complete.

P41896-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET
60 70 80 90 100
KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD
110 120 130 140 150
GSKITLLLNE NDNDSIPHEY DLELTKKVVE NEYVFTEQNL KKYQQRKKEL
160 170 180 190 200
EADPEKQRQA YLKKQEREEE LKKKQQQQKR RNNRKKFNHR VMTDRDGRDR
210 220 230 240 250
YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR RNIVKLNNKE
260 270 280 290 300
RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL
310 320 330 340 350
DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT
360 370 380 390 400
LRPEYKKLKE EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV
Length:400
Mass (Da):46,605
Last modified:October 1, 1996 - v2
Checksum:iDDDA895446BBF26F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2321N → H in AAA61642 (PubMed:7995524).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13016 Genomic DNA. Translation: AAA61642.1.
Z72790 Genomic DNA. Translation: CAA96988.1.
AY692871 Genomic DNA. Translation: AAT92890.1.
BK006941 Genomic DNA. Translation: DAA08103.1.
PIRiS64294.
RefSeqiNP_011519.3. NM_001181134.3.

Genome annotation databases

EnsemblFungiiYGR005C; YGR005C; YGR005C.
GeneIDi852888.
KEGGisce:YGR005C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13016 Genomic DNA. Translation: AAA61642.1.
Z72790 Genomic DNA. Translation: CAA96988.1.
AY692871 Genomic DNA. Translation: AAT92890.1.
BK006941 Genomic DNA. Translation: DAA08103.1.
PIRiS64294.
RefSeqiNP_011519.3. NM_001181134.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V1Nelectron microscopy7.80R1-400[»]
4V1Oelectron microscopy9.70R1-400[»]
ProteinModelPortaliP41896.
SMRiP41896. Positions 54-142, 293-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33249. 133 interactions.
DIPiDIP-1152N.
IntActiP41896. 21 interactions.
MINTiMINT-562451.
STRINGi4932.YGR005C.

Proteomic databases

MaxQBiP41896.
PaxDbiP41896.
PeptideAtlasiP41896.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR005C; YGR005C; YGR005C.
GeneIDi852888.
KEGGisce:YGR005C.

Organism-specific databases

CYGDiYGR005c.
EuPathDBiFungiDB:YGR005C.
SGDiS000003237. TFG2.

Phylogenomic databases

eggNOGiCOG5090.
GeneTreeiENSGT00390000016051.
HOGENOMiHOG000000947.
InParanoidiP41896.
KOiK03139.
OMAiLKERTRQ.
OrthoDBiEOG7VB2RC.

Enzyme and pathway databases

BioCyciYEAST:G3O-30736-MONOMER.
ReactomeiREACT_273109. Formation of the Early Elongation Complex.
REACT_277632. mRNA Capping.
REACT_294700. mRNA Splicing - Minor Pathway.
REACT_296055. RNA Polymerase II Promoter Escape.
REACT_300180. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_310018. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_315180. RNA Polymerase II Pre-transcription Events.
REACT_317581. Processing of Capped Intron-Containing Pre-mRNA.
REACT_322294. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_345860. RNA Polymerase II Transcription Initiation.

Miscellaneous databases

NextBioi972547.
PROiP41896.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003196. TFIIF_beta.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10445. PTHR10445. 1 hit.
PfamiPF02270. TFIIF_beta. 1 hit.
[Graphical view]
SUPFAMiSSF50916. SSF50916. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 250-279 AND 349-356.
    Strain: ATCC 208279 / BJ926.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Purification and characterization of yeast RNA polymerase II general initiation factor g."
    Henry N.L., Sayre M.H., Kornberg R.D.
    J. Biol. Chem. 267:23388-23392(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28 AND SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiT2FB_YEAST
AccessioniPrimary (citable) accession number: P41896
Secondary accession number(s): D6VUE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 520 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.