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Protein

Transcription initiation factor IIF subunit beta

Gene

TFG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TFG2 shows ATP-dependent DNA-helicase activity (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30736-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-72165. mRNA Splicing - Minor Pathway.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIF subunit beta (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase TFG2
TFIIF medium subunit
TFIIF-beta
Transcription factor G 54 kDa subunit
Gene namesi
Name:TFG2
Ordered Locus Names:YGR005C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR005C.
SGDiS000003237. TFG2.

Subcellular locationi

GO - Cellular componenti

  • transcription factor TFIIF complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002112401 – 400Transcription initiation factor IIF subunit betaAdd BLAST400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei56PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41896.
PRIDEiP41896.

PTM databases

iPTMnetiP41896.

Interactioni

Subunit structurei

TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.

Protein-protein interaction databases

BioGridi33249. 129 interactors.
DIPiDIP-1152N.
IntActiP41896. 22 interactors.
MINTiMINT-562451.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V1Nelectron microscopy7.80R1-400[»]
4V1Oelectron microscopy9.70R1-400[»]
5FMFelectron microscopy6.00V54-142[»]
5FYWelectron microscopy4.35R1-400[»]
5FZ5electron microscopy8.80R1-400[»]
5SVAelectron microscopy15.30g1-400[»]
ProteinModelPortaliP41896.
SMRiP41896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIF beta subunit family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016051.
HOGENOMiHOG000000947.
InParanoidiP41896.
KOiK03139.
OMAiAPREYPF.
OrthoDBiEOG092C4JNL.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003196. TFIIF_beta.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10445. PTHR10445. 2 hits.
PfamiPF02270. TFIIF_beta. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50916. SSF50916. 1 hit.

Sequencei

Sequence statusi: Complete.

P41896-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET
60 70 80 90 100
KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD
110 120 130 140 150
GSKITLLLNE NDNDSIPHEY DLELTKKVVE NEYVFTEQNL KKYQQRKKEL
160 170 180 190 200
EADPEKQRQA YLKKQEREEE LKKKQQQQKR RNNRKKFNHR VMTDRDGRDR
210 220 230 240 250
YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR RNIVKLNNKE
260 270 280 290 300
RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL
310 320 330 340 350
DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT
360 370 380 390 400
LRPEYKKLKE EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV
Length:400
Mass (Da):46,605
Last modified:October 1, 1996 - v2
Checksum:iDDDA895446BBF26F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232N → H in AAA61642 (PubMed:7995524).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13016 Genomic DNA. Translation: AAA61642.1.
Z72790 Genomic DNA. Translation: CAA96988.1.
AY692871 Genomic DNA. Translation: AAT92890.1.
BK006941 Genomic DNA. Translation: DAA08103.1.
PIRiS64294.
RefSeqiNP_011519.3. NM_001181134.3.

Genome annotation databases

EnsemblFungiiYGR005C; YGR005C; YGR005C.
GeneIDi852888.
KEGGisce:YGR005C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13016 Genomic DNA. Translation: AAA61642.1.
Z72790 Genomic DNA. Translation: CAA96988.1.
AY692871 Genomic DNA. Translation: AAT92890.1.
BK006941 Genomic DNA. Translation: DAA08103.1.
PIRiS64294.
RefSeqiNP_011519.3. NM_001181134.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V1Nelectron microscopy7.80R1-400[»]
4V1Oelectron microscopy9.70R1-400[»]
5FMFelectron microscopy6.00V54-142[»]
5FYWelectron microscopy4.35R1-400[»]
5FZ5electron microscopy8.80R1-400[»]
5SVAelectron microscopy15.30g1-400[»]
ProteinModelPortaliP41896.
SMRiP41896.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33249. 129 interactors.
DIPiDIP-1152N.
IntActiP41896. 22 interactors.
MINTiMINT-562451.

PTM databases

iPTMnetiP41896.

Proteomic databases

MaxQBiP41896.
PRIDEiP41896.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR005C; YGR005C; YGR005C.
GeneIDi852888.
KEGGisce:YGR005C.

Organism-specific databases

EuPathDBiFungiDB:YGR005C.
SGDiS000003237. TFG2.

Phylogenomic databases

GeneTreeiENSGT00390000016051.
HOGENOMiHOG000000947.
InParanoidiP41896.
KOiK03139.
OMAiAPREYPF.
OrthoDBiEOG092C4JNL.

Enzyme and pathway databases

BioCyciYEAST:G3O-30736-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-72086. mRNA Capping.
R-SCE-72165. mRNA Splicing - Minor Pathway.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiP41896.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003196. TFIIF_beta.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10445. PTHR10445. 2 hits.
PfamiPF02270. TFIIF_beta. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF50916. SSF50916. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2FB_YEAST
AccessioniPrimary (citable) accession number: P41896
Secondary accession number(s): D6VUE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 520 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.