P41896 (T2FB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor IIF subunit beta
EC=3.6.4.12

Alternative name(s):
ATP-dependent helicase TFG2
TFIIF medium subunit
TFIIF-beta
Transcription factor G 54 kDa subunit

Gene names

Name:	TFG2
Ordered Locus Names:	YGR005C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TFG2 shows ATP-dependent DNA-helicase activity By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.
Subcellular location	Nucleus.
Miscellaneous	Present with 520 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the TFIIF beta subunit family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	RNA polymerase II transcriptional preinitiation complex assembly Inferred by curator. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor TFIIF complex Inferred from direct assay Ref.1. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Transcription initiation factor IIF subunit beta		PRO_0000211240
Amino acid modifications
	Modified residue	28	1	Phosphoserine Ref.7
	Modified residue	34	1	Phosphoserine Ref.7
	Modified residue	56	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	232	1	N → H in AAA61642. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P41896 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: DDDA895446BBF26F	FASTA	400	46,605
	10 20 30 40 50 60 MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD 70 80 90 100 110 120 LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD GSKITLLLNE NDNDSIPHEY 130 140 150 160 170 180 DLELTKKVVE NEYVFTEQNL KKYQQRKKEL EADPEKQRQA YLKKQEREEE LKKKQQQQKR 190 200 210 220 230 240 RNNRKKFNHR VMTDRDGRDR YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR 250 260 270 280 290 300 RNIVKLNNKE RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL 310 320 330 340 350 360 DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT LRPEYKKLKE 370 380 390 400 EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TFIIF-TAF-RNA polymerase II connection." Henry N.L., Campbell A.M., Feaver W.J., Poon D., Weil P.A., Kornberg R.D. Genes Dev. 8:2868-2878(1994) [PubMed: 7995524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 250-279 AND 349-356. Strain: ATCC 208279 / BJ926.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Purification and characterization of yeast RNA polymerase II general initiation factor g." Henry N.L., Sayre M.H., Kornberg R.D. J. Biol. Chem. 267:23388-23392(1992) [PubMed: 1331085] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28; SER-34 AND SER-56, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13016 Genomic DNA. Translation: AAA61642.1. Z72790 Genomic DNA. Translation: CAA96988.1. AY692871 Genomic DNA. Translation: AAT92890.1. BK006941 Genomic DNA. Translation: DAA08103.1.
PIR	S64294.
RefSeq	NP_011519.1. NM_001181134.1.
3D structure databases
ProteinModelPortal	P41896.
SMR	P41896. Positions 291-359.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1152N.
IntAct	P41896. 21 interactions.
MINT	MINT-562451.
STRING	P41896.
Proteomic databases
PeptideAtlas	P41896.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR005C; YGR005C; YGR005C.
GeneID	852888.
KEGG	sce:YGR005C.
NMPDR	fig|4932.3.peg.2629.
Organism-specific databases
CYGD	YGR005c.
SGD	S000003237. TFG2.
Phylogenomic databases
eggNOG	fuNOG11091.
GeneTree	EFGT00050000005787.
HOGENOM	HBG396105.
OMA	KTIPKKT.
OrthoDB	EOG4Q886J.
Gene expression databases
ArrayExpress	P41896.
Genevestigator	P41896.
GermOnline	YGR005C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003196. TFIIF_beta. IPR011039. TFIIF_interaction. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K03139.
PANTHER	PTHR10445. TFIIF_beta. 1 hit.
Pfam	PF02270. TFIIF_beta. 1 hit. [Graphical view]
SUPFAM	SSF50916. TFIIF_interactn. 1 hit.
ProtoNet	Search...
Other
NextBio	972547.

Entry information

Entry name

T2FB_YEAST

Accession

Primary (citable) accession number: P41896
Secondary accession number(s): D6VUE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program