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P41896

- T2FB_YEAST

UniProt

P41896 - T2FB_YEAST

Protein

Transcription initiation factor IIF subunit beta

Gene

TFG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TFG2 shows ATP-dependent DNA-helicase activity By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. core RNA polymerase II binding transcription factor activity Source: SGD
    3. DNA binding Source: UniProtKB-KW
    4. helicase activity Source: UniProtKB-KW

    GO - Biological processi

    1. promoter clearance from RNA polymerase II promoter Source: SGD
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    4. transcriptional start site selection at RNA polymerase II promoter Source: SGD
    5. transcription elongation from RNA polymerase II promoter Source: SGD
    6. transcription initiation from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30736-MONOMER.
    ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor IIF subunit beta (EC:3.6.4.12)
    Alternative name(s):
    ATP-dependent helicase TFG2
    TFIIF medium subunit
    TFIIF-beta
    Transcription factor G 54 kDa subunit
    Gene namesi
    Name:TFG2
    Ordered Locus Names:YGR005C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR005c.
    SGDiS000003237. TFG2.

    Subcellular locationi

    GO - Cellular componenti

    1. transcription factor TFIIF complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Transcription initiation factor IIF subunit betaPRO_0000211240Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei28 – 281Phosphoserine1 Publication
    Modified residuei34 – 341Phosphoserine1 Publication
    Modified residuei56 – 561Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP41896.
    PaxDbiP41896.
    PeptideAtlasiP41896.

    Expressioni

    Gene expression databases

    GenevestigatoriP41896.

    Interactioni

    Subunit structurei

    TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.

    Protein-protein interaction databases

    BioGridi33249. 133 interactions.
    DIPiDIP-1152N.
    IntActiP41896. 21 interactions.
    MINTiMINT-562451.
    STRINGi4932.YGR005C.

    Structurei

    3D structure databases

    ProteinModelPortaliP41896.
    SMRiP41896. Positions 293-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TFIIF beta subunit family.Curated

    Phylogenomic databases

    eggNOGiCOG5090.
    GeneTreeiENSGT00390000016051.
    HOGENOMiHOG000000947.
    KOiK03139.
    OMAiYWSMKAL.
    OrthoDBiEOG7VB2RC.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR003196. TFIIF_beta.
    IPR011039. TFIIF_interaction.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10445. PTHR10445. 1 hit.
    PfamiPF02270. TFIIF_beta. 1 hit.
    [Graphical view]
    SUPFAMiSSF50916. SSF50916. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P41896-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET    50
    KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD 100
    GSKITLLLNE NDNDSIPHEY DLELTKKVVE NEYVFTEQNL KKYQQRKKEL 150
    EADPEKQRQA YLKKQEREEE LKKKQQQQKR RNNRKKFNHR VMTDRDGRDR 200
    YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR RNIVKLNNKE 250
    RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL 300
    DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT 350
    LRPEYKKLKE EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV 400
    Length:400
    Mass (Da):46,605
    Last modified:October 1, 1996 - v2
    Checksum:iDDDA895446BBF26F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti232 – 2321N → H in AAA61642. (PubMed:7995524)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U13016 Genomic DNA. Translation: AAA61642.1.
    Z72790 Genomic DNA. Translation: CAA96988.1.
    AY692871 Genomic DNA. Translation: AAT92890.1.
    BK006941 Genomic DNA. Translation: DAA08103.1.
    PIRiS64294.
    RefSeqiNP_011519.3. NM_001181134.3.

    Genome annotation databases

    EnsemblFungiiYGR005C; YGR005C; YGR005C.
    GeneIDi852888.
    KEGGisce:YGR005C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U13016 Genomic DNA. Translation: AAA61642.1 .
    Z72790 Genomic DNA. Translation: CAA96988.1 .
    AY692871 Genomic DNA. Translation: AAT92890.1 .
    BK006941 Genomic DNA. Translation: DAA08103.1 .
    PIRi S64294.
    RefSeqi NP_011519.3. NM_001181134.3.

    3D structure databases

    ProteinModelPortali P41896.
    SMRi P41896. Positions 293-356.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33249. 133 interactions.
    DIPi DIP-1152N.
    IntActi P41896. 21 interactions.
    MINTi MINT-562451.
    STRINGi 4932.YGR005C.

    Proteomic databases

    MaxQBi P41896.
    PaxDbi P41896.
    PeptideAtlasi P41896.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR005C ; YGR005C ; YGR005C .
    GeneIDi 852888.
    KEGGi sce:YGR005C.

    Organism-specific databases

    CYGDi YGR005c.
    SGDi S000003237. TFG2.

    Phylogenomic databases

    eggNOGi COG5090.
    GeneTreei ENSGT00390000016051.
    HOGENOMi HOG000000947.
    KOi K03139.
    OMAi YWSMKAL.
    OrthoDBi EOG7VB2RC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30736-MONOMER.
    Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

    Miscellaneous databases

    NextBioi 972547.
    PROi P41896.

    Gene expression databases

    Genevestigatori P41896.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR003196. TFIIF_beta.
    IPR011039. TFIIF_interaction.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10445. PTHR10445. 1 hit.
    Pfami PF02270. TFIIF_beta. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50916. SSF50916. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 250-279 AND 349-356.
      Strain: ATCC 208279 / BJ926.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Purification and characterization of yeast RNA polymerase II general initiation factor g."
      Henry N.L., Sayre M.H., Kornberg R.D.
      J. Biol. Chem. 267:23388-23392(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28 AND SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiT2FB_YEAST
    AccessioniPrimary (citable) accession number: P41896
    Secondary accession number(s): D6VUE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 520 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3