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P41896 (T2FB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor IIF subunit beta

EC=3.6.4.12
Alternative name(s):
ATP-dependent helicase TFG2
TFIIF medium subunit
TFIIF-beta
Transcription factor G 54 kDa subunit
Gene names
Name:TFG2
Ordered Locus Names:YGR005C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TFG2 shows ATP-dependent DNA-helicase activity By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.

Subcellular location

Nucleus.

Miscellaneous

Present with 520 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TFIIF beta subunit family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Transcription initiation factor IIF subunit beta
PRO_0000211240

Amino acid modifications

Modified residue281Phosphoserine Ref.8
Modified residue341Phosphoserine Ref.8
Modified residue561Phosphoserine Ref.7

Experimental info

Sequence conflict2321N → H in AAA61642. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P41896 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: DDDA895446BBF26F

FASTA40046,605
        10         20         30         40         50         60 
MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD 

        70         80         90        100        110        120 
LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD GSKITLLLNE NDNDSIPHEY 

       130        140        150        160        170        180 
DLELTKKVVE NEYVFTEQNL KKYQQRKKEL EADPEKQRQA YLKKQEREEE LKKKQQQQKR 

       190        200        210        220        230        240 
RNNRKKFNHR VMTDRDGRDR YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR 

       250        260        270        280        290        300 
RNIVKLNNKE RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL 

       310        320        330        340        350        360 
DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT LRPEYKKLKE 

       370        380        390        400 
EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV 

« Hide

References

« Hide 'large scale' references
[1]"TFIIF-TAF-RNA polymerase II connection."
Henry N.L., Campbell A.M., Feaver W.J., Poon D., Weil P.A., Kornberg R.D.
Genes Dev. 8:2868-2878(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 250-279 AND 349-356.
Strain: ATCC 208279 / BJ926.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Purification and characterization of yeast RNA polymerase II general initiation factor g."
Henry N.L., Sayre M.H., Kornberg R.D.
J. Biol. Chem. 267:23388-23392(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28 AND SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U13016 Genomic DNA. Translation: AAA61642.1.
Z72790 Genomic DNA. Translation: CAA96988.1.
AY692871 Genomic DNA. Translation: AAT92890.1.
BK006941 Genomic DNA. Translation: DAA08103.1.
PIRS64294.
RefSeqNP_011519.3. NM_001181134.3.

3D structure databases

ProteinModelPortalP41896.
SMRP41896. Positions 293-356.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33249. 133 interactions.
DIPDIP-1152N.
IntActP41896. 21 interactions.
MINTMINT-562451.
STRING4932.YGR005C.

Proteomic databases

PaxDbP41896.
PeptideAtlasP41896.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR005C; YGR005C; YGR005C.
GeneID852888.
KEGGsce:YGR005C.

Organism-specific databases

CYGDYGR005c.
SGDS000003237. TFG2.

Phylogenomic databases

eggNOGCOG5090.
GeneTreeENSGT00390000016051.
HOGENOMHOG000000947.
KOK03139.
OMAYWSMKAL.
OrthoDBEOG7VB2RC.

Enzyme and pathway databases

BioCycYEAST:G3O-30736-MONOMER.

Gene expression databases

GenevestigatorP41896.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR003196. TFIIF_beta.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR10445. PTHR10445. 1 hit.
PfamPF02270. TFIIF_beta. 1 hit.
[Graphical view]
SUPFAMSSF50916. SSF50916. 1 hit.
ProtoNetSearch...

Other

NextBio972547.
PROP41896.

Entry information

Entry nameT2FB_YEAST
AccessionPrimary (citable) accession number: P41896
Secondary accession number(s): D6VUE2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families