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Protein

Transcription initiation factor IIF subunit beta

Gene

TFG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TFG2 shows ATP-dependent DNA-helicase activity (By similarity).By similarity

Miscellaneous

Present with 520 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30736-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-72086 mRNA Capping
R-SCE-72165 mRNA Splicing - Minor Pathway
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-SCE-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIF subunit beta (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase TFG2
TFIIF medium subunit
TFIIF-beta
Transcription factor G 54 kDa subunit
Gene namesi
Name:TFG2
Ordered Locus Names:YGR005C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR005C
SGDiS000003237 TFG2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002112401 – 400Transcription initiation factor IIF subunit betaAdd BLAST400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei56PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41896
PaxDbiP41896
PRIDEiP41896

PTM databases

iPTMnetiP41896

Interactioni

Subunit structurei

TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33249, 243 interactors
DIPiDIP-1152N
IntActiP41896, 32 interactors
MINTiP41896
STRINGi4932.YGR005C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V1Nelectron microscopy7.80R1-400[»]
4V1Oelectron microscopy9.70R1-400[»]
5FMFelectron microscopy6.00V54-140[»]
V210-227[»]
V291-359[»]
5FYWelectron microscopy4.35R1-400[»]
5FZ5electron microscopy8.80R1-400[»]
5SVAelectron microscopy15.30g1-400[»]
ProteinModelPortaliP41896
SMRiP41896
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIF beta subunit family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016051
HOGENOMiHOG000000947
InParanoidiP41896
KOiK03139
OMAiKTTRMPQ
OrthoDBiEOG092C4JNL

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR003196 TFIIF_beta
IPR011039 TFIIF_interaction
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10445 PTHR10445, 1 hit
PfamiView protein in Pfam
PF02270 TFIIF_beta, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50916 SSF50916, 1 hit

Sequencei

Sequence statusi: Complete.

P41896-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET
60 70 80 90 100
KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD
110 120 130 140 150
GSKITLLLNE NDNDSIPHEY DLELTKKVVE NEYVFTEQNL KKYQQRKKEL
160 170 180 190 200
EADPEKQRQA YLKKQEREEE LKKKQQQQKR RNNRKKFNHR VMTDRDGRDR
210 220 230 240 250
YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR RNIVKLNNKE
260 270 280 290 300
RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL
310 320 330 340 350
DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT
360 370 380 390 400
LRPEYKKLKE EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV
Length:400
Mass (Da):46,605
Last modified:October 1, 1996 - v2
Checksum:iDDDA895446BBF26F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232N → H in AAA61642 (PubMed:7995524).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13016 Genomic DNA Translation: AAA61642.1
Z72790 Genomic DNA Translation: CAA96988.1
AY692871 Genomic DNA Translation: AAT92890.1
BK006941 Genomic DNA Translation: DAA08103.1
PIRiS64294
RefSeqiNP_011519.3, NM_001181134.3

Genome annotation databases

EnsemblFungiiYGR005C; YGR005C; YGR005C
GeneIDi852888
KEGGisce:YGR005C

Similar proteinsi

Entry informationi

Entry nameiT2FB_YEAST
AccessioniPrimary (citable) accession number: P41896
Secondary accession number(s): D6VUE2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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