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P41896

- T2FB_YEAST

UniProt

P41896 - T2FB_YEAST

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Protein

Transcription initiation factor IIF subunit beta

Gene
TFG2, YGR005C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. TFG2 shows ATP-dependent DNA-helicase activity By similarity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. core RNA polymerase II binding transcription factor activity Source: SGD
  3. DNA binding Source: UniProtKB-KW
  4. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. promoter clearance from RNA polymerase II promoter Source: SGD
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  4. transcriptional start site selection at RNA polymerase II promoter Source: SGD
  5. transcription elongation from RNA polymerase II promoter Source: SGD
  6. transcription initiation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30736-MONOMER.
ReactomeiREACT_191540. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIF subunit beta (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase TFG2
TFIIF medium subunit
TFIIF-beta
Transcription factor G 54 kDa subunit
Gene namesi
Name:TFG2
Ordered Locus Names:YGR005C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR005c.
SGDiS000003237. TFG2.

Subcellular locationi

GO - Cellular componenti

  1. transcription factor TFIIF complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Transcription initiation factor IIF subunit betaPRO_0000211240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281Phosphoserine1 Publication
Modified residuei34 – 341Phosphoserine1 Publication
Modified residuei56 – 561Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41896.
PaxDbiP41896.
PeptideAtlasiP41896.

Expressioni

Gene expression databases

GenevestigatoriP41896.

Interactioni

Subunit structurei

TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.

Protein-protein interaction databases

BioGridi33249. 133 interactions.
DIPiDIP-1152N.
IntActiP41896. 21 interactions.
MINTiMINT-562451.
STRINGi4932.YGR005C.

Structurei

3D structure databases

ProteinModelPortaliP41896.
SMRiP41896. Positions 293-356.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5090.
GeneTreeiENSGT00390000016051.
HOGENOMiHOG000000947.
KOiK03139.
OMAiYWSMKAL.
OrthoDBiEOG7VB2RC.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003196. TFIIF_beta.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10445. PTHR10445. 1 hit.
PfamiPF02270. TFIIF_beta. 1 hit.
[Graphical view]
SUPFAMiSSF50916. SSF50916. 1 hit.

Sequencei

Sequence statusi: Complete.

P41896-1 [UniParc]FASTAAdd to Basket

« Hide

MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET    50
KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD 100
GSKITLLLNE NDNDSIPHEY DLELTKKVVE NEYVFTEQNL KKYQQRKKEL 150
EADPEKQRQA YLKKQEREEE LKKKQQQQKR RNNRKKFNHR VMTDRDGRDR 200
YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR RNIVKLNNKE 250
RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL 300
DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT 350
LRPEYKKLKE EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV 400
Length:400
Mass (Da):46,605
Last modified:October 1, 1996 - v2
Checksum:iDDDA895446BBF26F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2321N → H in AAA61642. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U13016 Genomic DNA. Translation: AAA61642.1.
Z72790 Genomic DNA. Translation: CAA96988.1.
AY692871 Genomic DNA. Translation: AAT92890.1.
BK006941 Genomic DNA. Translation: DAA08103.1.
PIRiS64294.
RefSeqiNP_011519.3. NM_001181134.3.

Genome annotation databases

EnsemblFungiiYGR005C; YGR005C; YGR005C.
GeneIDi852888.
KEGGisce:YGR005C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U13016 Genomic DNA. Translation: AAA61642.1 .
Z72790 Genomic DNA. Translation: CAA96988.1 .
AY692871 Genomic DNA. Translation: AAT92890.1 .
BK006941 Genomic DNA. Translation: DAA08103.1 .
PIRi S64294.
RefSeqi NP_011519.3. NM_001181134.3.

3D structure databases

ProteinModelPortali P41896.
SMRi P41896. Positions 293-356.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33249. 133 interactions.
DIPi DIP-1152N.
IntActi P41896. 21 interactions.
MINTi MINT-562451.
STRINGi 4932.YGR005C.

Proteomic databases

MaxQBi P41896.
PaxDbi P41896.
PeptideAtlasi P41896.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR005C ; YGR005C ; YGR005C .
GeneIDi 852888.
KEGGi sce:YGR005C.

Organism-specific databases

CYGDi YGR005c.
SGDi S000003237. TFG2.

Phylogenomic databases

eggNOGi COG5090.
GeneTreei ENSGT00390000016051.
HOGENOMi HOG000000947.
KOi K03139.
OMAi YWSMKAL.
OrthoDBi EOG7VB2RC.

Enzyme and pathway databases

BioCyci YEAST:G3O-30736-MONOMER.
Reactomei REACT_191540. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi 972547.
PROi P41896.

Gene expression databases

Genevestigatori P41896.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR003196. TFIIF_beta.
IPR011039. TFIIF_interaction.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR10445. PTHR10445. 1 hit.
Pfami PF02270. TFIIF_beta. 1 hit.
[Graphical view ]
SUPFAMi SSF50916. SSF50916. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 250-279 AND 349-356.
    Strain: ATCC 208279 / BJ926.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Purification and characterization of yeast RNA polymerase II general initiation factor g."
    Henry N.L., Sayre M.H., Kornberg R.D.
    J. Biol. Chem. 267:23388-23392(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28 AND SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiT2FB_YEAST
AccessioniPrimary (citable) accession number: P41896
Secondary accession number(s): D6VUE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 520 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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