P41895 (T2FA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor IIF subunit alpha
Short name=TFIIF-alpha

Alternative name(s):
TFIIF large subunit
Transcription factor G 105 kDa subunit
Short name=P105

Gene names

Name:	TFG1
Synonyms:	SSU71
Ordered Locus Names:	YGR186W
ORF Names:	G7526

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. Ref.6
Subunit structure	TFIIF is composed of three different subunits: TFG1/RAP74, TFG2/RAP30 and TAF14.
Subcellular location	Nucleus.
Post-translational modification	Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases By similarity. Ref.8 Ref.9 Ref.10 Ref.11
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the TFIIF alpha subunit family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	RNA polymerase II transcriptional preinitiation complex assembly Inferred by curator. Source: GOC dephosphorylation of RNA polymerase II C-terminal domain Inferred from direct assay. Source: SGD positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro regulation of protein serine/threonine phosphatase activity Inferred from direct assay. Source: SGD
Cellular component	transcription factor TFIIF complex Inferred from direct assay Ref.1. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW catalytic activity Inferred from electronic annotation. Source: InterPro protein phosphatase activator activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Transcription initiation factor IIF subunit alpha		PRO_0000211234
Regions
	Compositional bias	455 – 580	126	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	198	1	Phosphoserine Ref.10 Ref.11
	Modified residue	200	1	Phosphothreonine Ref.8 Ref.11
	Modified residue	515	1	Phosphoserine Ref.8 Ref.10 Ref.11
	Modified residue	571	1	Phosphoserine Ref.9
	Modified residue	655	1	Phosphoserine Ref.8 Ref.10 Ref.11
	Modified residue	673	1	Phosphothreonine Ref.10 Ref.11
Experimental info
	Sequence conflict	238	1	V → I in AAA61640. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P41895 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: A6D4685F187BB593	FASTA	735	82,194
	10 20 30 40 50 60 MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK 70 80 90 100 110 120 KDDPEYAEER EKMLLQIGVE ADAGRSNVKV KDEDPNEYNE FPLRAIPKED LENMRTHLLK 130 140 150 160 170 180 FQSKKKINPV TDFHLPVRLH RKDTRNLQFQ LTRAEIVQRQ KEISEYKKKA EQERSTPNSG 190 200 210 220 230 240 GMNKSGTVSL NNTVKDGSQT PTVDSVTKDN TANGVNSSIP TVTGSSVPPA SPTTVSAVES 250 260 270 280 290 300 NGLSNGSTSA ANGLDGNAST ANLANGRPLV TKLEDAGPAE DPTKVGMVKY DGKEVTNEPE 310 320 330 340 350 360 FEEGTMDPLA DVAPDGGGRA KRGNLRRKTR QLKVLDENAK KLRFEEFYPW VMEDFDGYNT 370 380 390 400 410 420 WVGSYEAGNS DSYVLLSVED DGSFTMIPAD KVYKFTARNK YATLTIDEAE KRMDKKSGEV 430 440 450 460 470 480 PRWLMKHLDN IGTTTTRYDR TRRKLKAVAD QQAMDEDDRD DNSEVELDYD EEFADDEEAP 490 500 510 520 530 540 IIDGNEQENK ESEQRIKKEM LQANAMGLRD EEAPSENEED ELFGEKKIDE DGERIKKALQ 550 560 570 580 590 600 KTELAALYSS DENEINPYLS ESDIENKENE SPVKKEEDSD TLSKSKRSSP KKQQKKATNA 610 620 630 640 650 660 HVHKEPTLRV KSIKNCVIIL KGDKKILKSF PEGEWNPQTT KAVDSSNNAS NTVPSPIKQE 670 680 690 700 710 720 EGLNSTVAER EETPAPTITE KDIIEAIGDG KVNIKEFGKF IRRKYPGAEN KKLMFAIVKK 730 LCRKVGNDHM ELKKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TFIIF-TAF-RNA polymerase II connection." Henry N.L., Campbell A.M., Feaver W.J., Poon D., Weil P.A., Kornberg R.D. Genes Dev. 8:2868-2878(1994) [PubMed: 7995524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 125-142 AND 143-161. Strain: ATCC 208279 / BJ926.
[2]	"Identification of the gene (SSU71/TFG1) encoding the largest subunit of transcription factor TFIIF as a suppressor of a TFIIB mutation in Saccharomyces cerevisiae." Sun Z.W., Hampsey M. Proc. Natl. Acad. Sci. U.S.A. 92:3127-3131(1995) [PubMed: 7724527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII." Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C. Yeast 13:357-363(1997) [PubMed: 9133739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Purification and characterization of yeast RNA polymerase II general initiation factor g." Henry N.L., Sayre M.H., Kornberg R.D. J. Biol. Chem. 267:23388-23392(1992) [PubMed: 1331085] [Abstract] Cited for: FUNCTION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-200; SER-515 AND SER-655, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-571, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198; SER-515; SER-655 AND THR-673, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198; THR-200; SER-515; SER-655 AND THR-673, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13015 Genomic DNA. Translation: AAA61640.1. U14527 Genomic DNA. Translation: AAA68478.1. X99074 Genomic DNA. Translation: CAA67530.1. Z72971 Genomic DNA. Translation: CAA97212.1. BK006941 Genomic DNA. Translation: DAA08280.1.
PIR	S64504.
RefSeq	NP_011702.1. NM_001181315.1.
3D structure databases
ProteinModelPortal	P41895.
SMR	P41895. Positions 344-417.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1151N.
IntAct	P41895. 13 interactions.
MINT	MINT-614625.
STRING	P41895.
Proteomic databases
PeptideAtlas	P41895.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR186W; YGR186W; YGR186W.
GeneID	853098.
KEGG	sce:YGR186W.
NMPDR	fig|4932.3.peg.2829.
Organism-specific databases
CYGD	YGR186w.
SGD	S000003418. TFG1.
Phylogenomic databases
eggNOG	fuNOG08967.
GeneTree	EFGT00050000000437.
HOGENOM	HBG397129.
OMA	ESEQRIK.
OrthoDB	EOG422DTW.
Gene expression databases
ArrayExpress	P41895.
Genevestigator	P41895.
GermOnline	YGR186W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008851. TFIIF-alpha. IPR011039. TFIIF_interaction. [Graphical view]
KO	K03138.
Pfam	PF05793. TFIIF_alpha. 1 hit. [Graphical view]
SUPFAM	SSF50916. TFIIF_interactn. 1 hit.
ProtoNet	Search...
Other
NextBio	973093.

Entry information

Entry name

T2FA_YEAST

Accession

Primary (citable) accession number: P41895
Secondary accession number(s): D6VUW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program