P41888 (TNR3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamine pyrophosphokinase
Short name=TPK
Short name=Thiamine kinase
EC=2.7.6.2

Gene names

Name:	tnr3
ORF Names:	SPAC6F12.05c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the regulation of thiamine metabolism, phosphate metabolism, mating and cell growth. Ref.3
Catalytic activity	ATP + thiamine = AMP + thiamine diphosphate.
Subcellular location	Cytoplasm Ref.4.
Sequence similarities	Contains 1 nudix hydrolase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding Thiamine pyrophosphate
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of thiamine biosynthetic process Inferred from mutant phenotype. Source: GeneDB_Spombe thiamine diphosphate biosynthetic process Inferred by curator. Source: GeneDB_Spombe thiamine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphokinase activity Inferred from genetic interaction. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Thiamine pyrophosphokinase		PRO_0000072610
Regions
	Domain	136 – 276	141	Nudix hydrolase

Sequences

	Sequence		Length	Mass (Da)
	P41888 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5118042478DF00FC	FASTA	569	64,236
	10 20 30 40 50 60 MAMSSITSAK QLNAEELLDE CDSFNGEFVP GTIPFRANGA AIGYVTPLVL EILIKADNFK 70 80 90 100 110 120 FNWVYVPGEY IEINASTFEK RTDILAKVLE HWRHNNTFGI ADQWRNELYT VYGKSKKPVL 130 140 150 160 170 180 AVERGGFWLF GFLSTGVHCT MYIPATKEHP LRIWVPRRSP TKQTWPNYLD NSVAGGIAHG 190 200 210 220 230 240 DSVIGTMIKE FSEEANLDVS SMNLIPCGTV SYIKMEKRHW IQPELQYVFD LPVDDLVIPR 250 260 270 280 290 300 INDGEVAGFS LLPLNQVLHE LELKSFKPNC ALVLLDFLIR HGIITPQHPQ YLQTLERIHR 310 320 330 340 350 360 PLPVPVGKYE RGDSFEDTSK KAETCVPAKP QKATHQLAPC KAWLRDYDTD QKFAVLLLNQ 370 380 390 400 410 420 PIDIPDDRFR TLWKRASIRV CADGGANQLR NYDSSLKPDY VVGDFDSLTD ETKAYYKEMG 430 440 450 460 470 480 VNIVFDPCQN TTDFMKCHKI IKEHGIDTIF VLCGMGGRVD HAIGNLNHLF WAASISEKNE 490 500 510 520 530 540 VFLLTELNVS TLLQPGINHV DCHDNIGLHC GLLPVGQSVY VKKTSGLEWN IEDRICQFGG 550 560 LVSSCNVVTK ATVTIEVNNF IVWTMETRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Schizosaccharomyces pombe thiamine pyrophosphokinase is encoded by gene tnr3 and is a regulator of thiamine metabolism, phosphate metabolism, mating, and growth." Fankhauser H., Zurlinden A., Schweingruber A.-M., Edenharter E., Schweingruber M.E. J. Biol. Chem. 270:28457-28462(1995) [PubMed: 7499352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Isolation and characterization of regulatory mutants from Schizosaccharomyces pombe involved in thiamine-regulated gene expression." Schweingruber A.-M., Fankhauser H., Dlugonski J., Steinmann-Loss C., Schweingruber M.E. Genetics 130:445-449(1992) [PubMed: 1551569] [Abstract] Cited for: FUNCTION.
[4]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X84417 Genomic DNA. Translation: CAA59135.1. CU329670 Genomic DNA. Translation: CAB11089.1.
PIR	S52350.
RefSeq	NP_593291.1. NM_001018721.1.
3D structure databases
ProteinModelPortal	P41888.
ModBase	Search...
Protein-protein interaction databases
STRING	P41888.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC6F12.05c.1; SPAC6F12.05c.1:pep; SPAC6F12.05c.
GeneID	2543032.
GenomeReviews	Gene locus tnr3 in contig CU329670_GR.
KEGG	spo:SPAC6F12.05c.
NMPDR	fig|4896.1.peg.3261.
Organism-specific databases
GeneDB_Spombe	SPAC6F12.05c.
Phylogenomic databases
eggNOG	fuNOG06534.
GeneTree	EFGT00050000003517.
OrthoDB	EOG4JMC07.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000990-MONOMER.
Gene expression databases
ArrayExpress	P41888.
Family and domain databases
InterPro	IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. IPR006282. Thi_PPkinase. IPR007373. Thiamin_PyroPKinase_B1-bd. IPR007371. TPK_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.120.320. G3DSA:2.60.120.320. 1 hit. G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit. G3DSA:3.40.50.10240. TPK_catalytic. 1 hit.
KO	K00949.
Pfam	PF00293. NUDIX. 1 hit. PF04265. TPK_B1_binding. 1 hit. PF04263. TPK_catalytic. 1 hit. [Graphical view]
SMART	SM00983. TPK_B1_binding. 1 hit. [Graphical view]
SUPFAM	SSF55811. NUDIX_hydrolase. 1 hit. SSF63862. TPK_B1_bd. 1 hit. SSF63999. TPK_catalytic. 1 hit.
TIGRFAMs	TIGR01378. Thi_PPkinase. 1 hit.
PROSITE	PS51462. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TNR3_SCHPO

Accession

Primary (citable) accession number: P41888

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program