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Protein

Thiamine pyrophosphokinase

Gene

tnr3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of thiamine metabolism, phosphate metabolism, mating and cell growth.1 Publication

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

GO - Molecular functioni

  1. 8-oxo-dGDP phosphatase activity Source: PomBase
  2. ATP binding Source: PomBase
  3. kinase activity Source: UniProtKB-KW
  4. magnesium ion binding Source: PomBase
  5. thiamine binding Source: PomBase
  6. thiamine diphosphokinase activity Source: PomBase

GO - Biological processi

  1. thiamine biosynthetic process Source: PomBase
  2. thiamine diphosphate biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Thiamine pyrophosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase (EC:2.7.6.2)
Short name:
TPK
Short name:
Thiamine kinase
Gene namesi
Name:tnr3
ORF Names:SPAC6F12.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC6F12.05c.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Thiamine pyrophosphokinasePRO_0000072610Add
BLAST

Proteomic databases

MaxQBiP41888.
PRIDEiP41888.

Interactioni

Protein-protein interaction databases

MINTiMINT-4690210.
STRINGi4896.SPAC6F12.05c-1.

Structurei

3D structure databases

ProteinModelPortaliP41888.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini136 – 276141Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0494.
InParanoidiP41888.
KOiK00949.
OrthoDBiEOG708W8M.
PhylomeDBiP41888.

Family and domain databases

Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
3.90.79.10. 1 hit.
InterProiIPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
IPR006282. Thi_PPkinase.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
PF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
SSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41888-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMSSITSAK QLNAEELLDE CDSFNGEFVP GTIPFRANGA AIGYVTPLVL
60 70 80 90 100
EILIKADNFK FNWVYVPGEY IEINASTFEK RTDILAKVLE HWRHNNTFGI
110 120 130 140 150
ADQWRNELYT VYGKSKKPVL AVERGGFWLF GFLSTGVHCT MYIPATKEHP
160 170 180 190 200
LRIWVPRRSP TKQTWPNYLD NSVAGGIAHG DSVIGTMIKE FSEEANLDVS
210 220 230 240 250
SMNLIPCGTV SYIKMEKRHW IQPELQYVFD LPVDDLVIPR INDGEVAGFS
260 270 280 290 300
LLPLNQVLHE LELKSFKPNC ALVLLDFLIR HGIITPQHPQ YLQTLERIHR
310 320 330 340 350
PLPVPVGKYE RGDSFEDTSK KAETCVPAKP QKATHQLAPC KAWLRDYDTD
360 370 380 390 400
QKFAVLLLNQ PIDIPDDRFR TLWKRASIRV CADGGANQLR NYDSSLKPDY
410 420 430 440 450
VVGDFDSLTD ETKAYYKEMG VNIVFDPCQN TTDFMKCHKI IKEHGIDTIF
460 470 480 490 500
VLCGMGGRVD HAIGNLNHLF WAASISEKNE VFLLTELNVS TLLQPGINHV
510 520 530 540 550
DCHDNIGLHC GLLPVGQSVY VKKTSGLEWN IEDRICQFGG LVSSCNVVTK
560
ATVTIEVNNF IVWTMETRL
Length:569
Mass (Da):64,236
Last modified:November 1, 1995 - v1
Checksum:i5118042478DF00FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84417 Genomic DNA. Translation: CAA59135.1.
CU329670 Genomic DNA. Translation: CAB11089.1.
PIRiS52350.
RefSeqiNP_593291.1. NM_001018721.2.

Genome annotation databases

EnsemblFungiiSPAC6F12.05c.1; SPAC6F12.05c.1:pep; SPAC6F12.05c.
GeneIDi2543032.
KEGGispo:SPAC6F12.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84417 Genomic DNA. Translation: CAA59135.1.
CU329670 Genomic DNA. Translation: CAB11089.1.
PIRiS52350.
RefSeqiNP_593291.1. NM_001018721.2.

3D structure databases

ProteinModelPortaliP41888.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4690210.
STRINGi4896.SPAC6F12.05c-1.

Proteomic databases

MaxQBiP41888.
PRIDEiP41888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6F12.05c.1; SPAC6F12.05c.1:pep; SPAC6F12.05c.
GeneIDi2543032.
KEGGispo:SPAC6F12.05c.

Organism-specific databases

PomBaseiSPAC6F12.05c.

Phylogenomic databases

eggNOGiCOG0494.
InParanoidiP41888.
KOiK00949.
OrthoDBiEOG708W8M.
PhylomeDBiP41888.

Miscellaneous databases

NextBioi20804063.

Family and domain databases

Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
3.90.79.10. 1 hit.
InterProiIPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
IPR006282. Thi_PPkinase.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
PF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
SSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Schizosaccharomyces pombe thiamine pyrophosphokinase is encoded by gene tnr3 and is a regulator of thiamine metabolism, phosphate metabolism, mating, and growth."
    Fankhauser H., Zurlinden A., Schweingruber A.-M., Edenharter E., Schweingruber M.E.
    J. Biol. Chem. 270:28457-28462(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Isolation and characterization of regulatory mutants from Schizosaccharomyces pombe involved in thiamine-regulated gene expression."
    Schweingruber A.-M., Fankhauser H., Dlugonski J., Steinmann-Loss C., Schweingruber M.E.
    Genetics 130:445-449(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTNR3_SCHPO
AccessioniPrimary (citable) accession number: P41888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.