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Protein

26S proteasome regulatory subunit rpn11

Gene

rpn11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.1 Publication
Transcription factor pap1 is controlled by the functional interaction between the positive regulator pad1 and negative regulator crm1. Both these proteins are also essential for cell viability and for the maintenance of chromosome structure. Pad1 is also responsible for resistance to staurosporine, and other drugs such as cycloheximide and caffeine.1 Publication

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: PomBase

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Cycloheximide resistance

Enzyme and pathway databases

ReactomeiR-SPO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit rpn11
Alternative name(s):
Protein pad1
Gene namesi
Name:rpn11
Synonyms:bfr2, pad1, sks1
ORF Names:SPAC31G5.13
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC31G5.13.
PomBaseiSPAC31G5.13. rpn11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • nuclear periphery Source: PomBase
  • nuclear proteasome complex Source: PomBase
  • nucleus Source: PomBase
  • proteasome regulatory particle, lid subcomplex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 30830826S proteasome regulatory subunit rpn11PRO_0000213959Add
BLAST

Proteomic databases

MaxQBiP41878.
PRIDEiP41878.

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity).By similarity

Protein-protein interaction databases

BioGridi279475. 47 interactions.
IntActiP41878. 1 interaction.
MINTiMINT-4690174.

Structurei

3D structure databases

ProteinModelPortaliP41878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M67A family.Curated
Contains 1 MPN (JAB/Mov34) domain.Curated

Phylogenomic databases

HOGENOMiHOG000183690.
InParanoidiP41878.
KOiK03030.
OMAiTQQSFES.
OrthoDBiEOG7RV9S0.
PhylomeDBiP41878.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41878-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLQRLLQG ARMGTGMMGD QPLVDNSECV YISSLALLKM LRHGRHGTPM
60 70 80 90 100
EVMGLMLGEF VDDFTVRVVD VFAMPQSGTG VSVEAVDPVF QKNMMDMLKQ
110 120 130 140 150
TGRPEMVVGW YHSHPGFGCW LSSVDINTQQ SFEQLTPRAV AVVVDPIQSV
160 170 180 190 200
KGKVVIDAFR LINPSTLMMG QEPRQTTSNL GHINKPSIQA LIHGLGRHYY
210 220 230 240 250
SLRINYKKTE LEEIMLLNLH KQPWAHGLLL ENFNSAAEKN HASIDKMKSL
260 270 280 290 300
SEQYTERVQN EVTLSPEQLR IQYVGKQDPK KHLDAEVQKC IDNNISSMLA

CMLDSVAF
Length:308
Mass (Da):34,572
Last modified:July 15, 1998 - v2
Checksum:i57E27C556B34E214
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121H → N in BAA06529 (PubMed:7642144).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31731 Genomic DNA. Translation: BAA06529.1.
D45047 Genomic DNA. Translation: BAA08087.1.
D84656 Genomic DNA. Translation: BAA12708.1.
CU329670 Genomic DNA. Translation: CAB11697.1.
PIRiT43293.
T44427.
RefSeqiNP_594014.1. NM_001019440.2.

Genome annotation databases

EnsemblFungiiSPAC31G5.13.1; SPAC31G5.13.1:pep; SPAC31G5.13.
GeneIDi2543040.
KEGGispo:SPAC31G5.13.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31731 Genomic DNA. Translation: BAA06529.1.
D45047 Genomic DNA. Translation: BAA08087.1.
D84656 Genomic DNA. Translation: BAA12708.1.
CU329670 Genomic DNA. Translation: CAB11697.1.
PIRiT43293.
T44427.
RefSeqiNP_594014.1. NM_001019440.2.

3D structure databases

ProteinModelPortaliP41878.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279475. 47 interactions.
IntActiP41878. 1 interaction.
MINTiMINT-4690174.

Proteomic databases

MaxQBiP41878.
PRIDEiP41878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC31G5.13.1; SPAC31G5.13.1:pep; SPAC31G5.13.
GeneIDi2543040.
KEGGispo:SPAC31G5.13.

Organism-specific databases

EuPathDBiFungiDB:SPAC31G5.13.
PomBaseiSPAC31G5.13. rpn11.

Phylogenomic databases

HOGENOMiHOG000183690.
InParanoidiP41878.
KOiK03030.
OMAiTQQSFES.
OrthoDBiEOG7RV9S0.
PhylomeDBiP41878.

Enzyme and pathway databases

ReactomeiR-SPO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SPO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiP41878.

Family and domain databases

InterProiIPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel essential fission yeast gene pad1+ positively regulates pap1(+)-dependent transcription and is implicated in the maintenance of chromosome structure."
    Shimanuki M., Saka Y., Yanagida M., Toda T.
    J. Cell Sci. 108:569-579(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A K-252a-resistance gene, sks1+, encodes a protein similar to the Caenorhabditis elegans F37 A4.5 gene product and confers multidrug resistance in Schizosaccharomyces pombe."
    Usui T., Yoshida M., Honda A., Beppu T., Horinouchi S.
    Gene 161:93-96(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of a 9.1 kb DNA fragment of Schizosaccharomyces pombe chromosome reveals the presence of pad1+/sks1+ gene and three previously unknown open reading frames."
    Nagao K., Arioka M., Kadokura H., Yoda K., Yamasaki M.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  5. Cited for: IDENTIFICATION AS A 26S PROTEASOME SUBUNIT, FUNCTION.

Entry informationi

Entry nameiRPN11_SCHPO
AccessioniPrimary (citable) accession number: P41878
Secondary accession number(s): Q10278
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.