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P41835

- THI6_YEAST

UniProt

P41835 - THI6_YEAST

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Protein
Thiamine biosynthetic bifunctional enzyme
Gene
THI6, YPL214C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Essential for thiamine biosynthesis. The kinase activity is involved in the salvage synthesis of TH-P from the thiazole.UniRule annotation
Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) By similarity.UniRule annotation

Catalytic activityi

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.UniRule annotation
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751HMP-PP By similarity
Metal bindingi76 – 761Magnesium By similarity
Metal bindingi95 – 951Magnesium By similarity
Binding sitei114 – 1141HMP-PP By similarity
Binding sitei146 – 1461HMP-PP By similarity
Binding sitei181 – 1811THZ-P; via amide nitrogen By similarity
Binding sitei290 – 29014-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogen By similarity
Binding sitei365 – 3651ATP By similarity
Binding sitei415 – 4151ATP By similarity
Binding sitei462 – 46214-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogen By similarity
Active sitei465 – 4651Proton acceptor; for hydroxyethylthiazole kinase activity By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxyethylthiazole kinase activity Source: SGD
  3. metal ion binding Source: UniProtKB-KW
  4. thiamine-phosphate diphosphorylase activity Source: SGD

GO - Biological processi

  1. thiamine biosynthetic process Source: SGD
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YPL214C-MONOMER.
YEAST:YPL214C-MONOMER.
UniPathwayiUPA00060; UER00139.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthetic bifunctional enzyme
Including the following 2 domains:
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Short name:
TMP-PPase
Hydroxyethylthiazole kinase (EC:2.7.1.50)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
THZ kinase
Gene namesi
Name:THI6
Ordered Locus Names:YPL214C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL214c.
SGDiS000006135. THI6.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540Thiamine biosynthetic bifunctional enzymeUniRule annotation
PRO_0000157090Add
BLAST

Proteomic databases

MaxQBiP41835.
PaxDbiP41835.

Expressioni

Gene expression databases

GenevestigatoriP41835.

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

BioGridi35971. 46 interactions.
DIPiDIP-1666N.
IntActiP41835. 1 interaction.
MINTiMINT-403731.
STRINGi4932.YPL214C.

Structurei

3D structure databases

ProteinModelPortaliP41835.
SMRiP41835. Positions 3-536.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 238238Thiamine-phosphate synthaseUniRule annotation
Add
BLAST
Regioni43 – 475HMP-PP binding By similarity
Regioni143 – 1453THZ-P binding By similarity
Regioni209 – 2102THZ-P binding By similarity
Regioni239 – 540302Hydroxyethylthiazole kinaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the thiamine-phosphate synthase family.
In the C-terminal section; belongs to the Thz kinase family.

Phylogenomic databases

eggNOGiCOG0352.
HOGENOMiHOG000214306.
KOiK14154.
OMAiNLVVQNF.
OrthoDBiEOG7KDFMZ.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
InterProiIPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.
TIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

P41835-1 [UniParc]FASTAAdd to Basket

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MVFTKEEVDY SLYLVTDSTM LPPGTTLCSQ VEAGLKNGVT LVQIREKDIE    50
TKNFVAEALE VQKICKKYNV PLIINDRIDV AMAIDADGVH VGQDDMPIPM 100
VRKLLGPSKI LGWSVGKPSE VETLAKWGPD MVDYIGVGTL FPTSTKKNPK 150
KSPMGPQGAI AILDALEEFK ATWCRTVGIG GLHPDNIQRV LCQCVASNGK 200
RSLDGISLVS DIMAAPDACA ATKRLRGLLD ATRYQFVECE LNNTFPTTTS 250
IQNVISQVSN NRPLVQHITN KVHQNFGANV TLALGSSPIM SEIESEVSEL 300
ARIPNASLLL NTGSVAPIEM LKAAINAYNE VNRPITFDPV GYSATETRLC 350
LNNTLLTYGQ FACIKGNCSE ILSLAKLNNH KMKGVDSSSG KTNIDTLVRA 400
TQIVAFQYRT VAVCTGEFDC VADGTFGGEY KLSSGTEGIT AEDLPCVIIE 450
DGPIPIMGDI TASGCSLGST IASFIGGLDS TGKLFDAVVG AVLLYKSAGK 500
LASTRCQGSG SFHVELIDAL YQLFHENKPE KWSASLKKFK 540
Length:540
Mass (Da):58,059
Last modified:November 1, 1995 - v1
Checksum:iF34FA1E0B76E3930
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti370 – 3701E → K in THI6-3; possesses TMP-PPASE activity only.
Natural varianti476 – 4761G → D in THI6-2; both enzyme activities greatly decreased.
Natural varianti510 – 5101G → D in THI6-1; both enzyme activities greatly decreased.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D31908 Genomic DNA. Translation: BAA06703.1.
Z73570 Genomic DNA. Translation: CAA97929.1.
BK006949 Genomic DNA. Translation: DAA11222.1.
PIRiA55145.
RefSeqiNP_015110.1. NM_001184028.1.

Genome annotation databases

EnsemblFungiiYPL214C; YPL214C; YPL214C.
GeneIDi855887.
KEGGisce:YPL214C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D31908 Genomic DNA. Translation: BAA06703.1 .
Z73570 Genomic DNA. Translation: CAA97929.1 .
BK006949 Genomic DNA. Translation: DAA11222.1 .
PIRi A55145.
RefSeqi NP_015110.1. NM_001184028.1.

3D structure databases

ProteinModelPortali P41835.
SMRi P41835. Positions 3-536.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35971. 46 interactions.
DIPi DIP-1666N.
IntActi P41835. 1 interaction.
MINTi MINT-403731.
STRINGi 4932.YPL214C.

Proteomic databases

MaxQBi P41835.
PaxDbi P41835.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL214C ; YPL214C ; YPL214C .
GeneIDi 855887.
KEGGi sce:YPL214C.

Organism-specific databases

CYGDi YPL214c.
SGDi S000006135. THI6.

Phylogenomic databases

eggNOGi COG0352.
HOGENOMi HOG000214306.
KOi K14154.
OMAi NLVVQNF.
OrthoDBi EOG7KDFMZ.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
UPA00060 ; UER00141 .
BioCyci MetaCyc:YPL214C-MONOMER.
YEAST:YPL214C-MONOMER.

Miscellaneous databases

NextBioi 980553.

Gene expression databases

Genevestigatori P41835.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPi MF_00097. TMP_synthase.
InterProi IPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view ]
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00693. thiE. 1 hit.
TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the THI6 gene encoding a bifunctional thiamin-phosphate pyrophosphorylase/hydroxyethylthiazole kinase from Saccharomyces cerevisiae."
    Nosaka K., Nishimura H., Kawasaki Y., Tsujihara T., Iwashima A.
    J. Biol. Chem. 269:30510-30516(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ND5-5A.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiTHI6_YEAST
AccessioniPrimary (citable) accession number: P41835
Secondary accession number(s): D6W3F6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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