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P41835

- THI6_YEAST

UniProt

P41835 - THI6_YEAST

Protein

Thiamine biosynthetic bifunctional enzyme

Gene

THI6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Essential for thiamine biosynthesis. The kinase activity is involved in the salvage synthesis of TH-P from the thiazole.
    Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).By similarity

    Catalytic activityi

    2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.
    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei75 – 751HMP-PPBy similarity
    Metal bindingi76 – 761MagnesiumBy similarity
    Metal bindingi95 – 951MagnesiumBy similarity
    Binding sitei114 – 1141HMP-PPBy similarity
    Binding sitei146 – 1461HMP-PPBy similarity
    Binding sitei181 – 1811THZ-P; via amide nitrogenBy similarity
    Binding sitei290 – 29014-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity
    Binding sitei365 – 3651ATPBy similarity
    Binding sitei415 – 4151ATPBy similarity
    Binding sitei462 – 46214-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity
    Active sitei465 – 4651Proton acceptor; for hydroxyethylthiazole kinase activityBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydroxyethylthiazole kinase activity Source: SGD
    3. metal ion binding Source: UniProtKB-KW
    4. thiamine-phosphate diphosphorylase activity Source: SGD

    GO - Biological processi

    1. thiamine biosynthetic process Source: SGD
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YPL214C-MONOMER.
    YEAST:YPL214C-MONOMER.
    UniPathwayiUPA00060; UER00139.
    UPA00060; UER00141.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiamine biosynthetic bifunctional enzyme
    Including the following 2 domains:
    Thiamine-phosphate synthase (EC:2.5.1.3)
    Short name:
    TP synthase
    Short name:
    TPS
    Alternative name(s):
    Thiamine-phosphate pyrophosphorylase
    Short name:
    TMP pyrophosphorylase
    Short name:
    TMP-PPase
    Hydroxyethylthiazole kinase (EC:2.7.1.50)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
    Short name:
    TH kinase
    Short name:
    THZ kinase
    Gene namesi
    Name:THI6
    Ordered Locus Names:YPL214C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL214c.
    SGDiS000006135. THI6.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 540540Thiamine biosynthetic bifunctional enzymePRO_0000157090Add
    BLAST

    Proteomic databases

    MaxQBiP41835.
    PaxDbiP41835.

    Expressioni

    Gene expression databases

    GenevestigatoriP41835.

    Interactioni

    Subunit structurei

    Homooctamer.

    Protein-protein interaction databases

    BioGridi35971. 46 interactions.
    DIPiDIP-1666N.
    IntActiP41835. 1 interaction.
    MINTiMINT-403731.
    STRINGi4932.YPL214C.

    Structurei

    3D structure databases

    ProteinModelPortaliP41835.
    SMRiP41835. Positions 3-536.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 238238Thiamine-phosphate synthaseAdd
    BLAST
    Regioni43 – 475HMP-PP bindingBy similarity
    Regioni143 – 1453THZ-P bindingBy similarity
    Regioni209 – 2102THZ-P bindingBy similarity
    Regioni239 – 540302Hydroxyethylthiazole kinaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the thiamine-phosphate synthase family.Curated
    In the C-terminal section; belongs to the Thz kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0352.
    HOGENOMiHOG000214306.
    KOiK14154.
    OMAiNLVVQNF.
    OrthoDBiEOG7KDFMZ.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.40.1190.20. 1 hit.
    HAMAPiMF_00097. TMP_synthase.
    InterProiIPR013785. Aldolase_TIM.
    IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    IPR022998. ThiaminP_synth_SF.
    IPR003733. TMP_synthase.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    PF02581. TMP-TENI. 1 hit.
    [Graphical view]
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF51391. SSF51391. 1 hit.
    SSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00693. thiE. 1 hit.
    TIGR00694. thiM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P41835-1 [UniParc]FASTAAdd to Basket

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    MVFTKEEVDY SLYLVTDSTM LPPGTTLCSQ VEAGLKNGVT LVQIREKDIE    50
    TKNFVAEALE VQKICKKYNV PLIINDRIDV AMAIDADGVH VGQDDMPIPM 100
    VRKLLGPSKI LGWSVGKPSE VETLAKWGPD MVDYIGVGTL FPTSTKKNPK 150
    KSPMGPQGAI AILDALEEFK ATWCRTVGIG GLHPDNIQRV LCQCVASNGK 200
    RSLDGISLVS DIMAAPDACA ATKRLRGLLD ATRYQFVECE LNNTFPTTTS 250
    IQNVISQVSN NRPLVQHITN KVHQNFGANV TLALGSSPIM SEIESEVSEL 300
    ARIPNASLLL NTGSVAPIEM LKAAINAYNE VNRPITFDPV GYSATETRLC 350
    LNNTLLTYGQ FACIKGNCSE ILSLAKLNNH KMKGVDSSSG KTNIDTLVRA 400
    TQIVAFQYRT VAVCTGEFDC VADGTFGGEY KLSSGTEGIT AEDLPCVIIE 450
    DGPIPIMGDI TASGCSLGST IASFIGGLDS TGKLFDAVVG AVLLYKSAGK 500
    LASTRCQGSG SFHVELIDAL YQLFHENKPE KWSASLKKFK 540
    Length:540
    Mass (Da):58,059
    Last modified:November 1, 1995 - v1
    Checksum:iF34FA1E0B76E3930
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti370 – 3701E → K in THI6-3; possesses TMP-PPASE activity only.
    Natural varianti476 – 4761G → D in THI6-2; both enzyme activities greatly decreased.
    Natural varianti510 – 5101G → D in THI6-1; both enzyme activities greatly decreased.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31908 Genomic DNA. Translation: BAA06703.1.
    Z73570 Genomic DNA. Translation: CAA97929.1.
    BK006949 Genomic DNA. Translation: DAA11222.1.
    PIRiA55145.
    RefSeqiNP_015110.1. NM_001184028.1.

    Genome annotation databases

    EnsemblFungiiYPL214C; YPL214C; YPL214C.
    GeneIDi855887.
    KEGGisce:YPL214C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31908 Genomic DNA. Translation: BAA06703.1 .
    Z73570 Genomic DNA. Translation: CAA97929.1 .
    BK006949 Genomic DNA. Translation: DAA11222.1 .
    PIRi A55145.
    RefSeqi NP_015110.1. NM_001184028.1.

    3D structure databases

    ProteinModelPortali P41835.
    SMRi P41835. Positions 3-536.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35971. 46 interactions.
    DIPi DIP-1666N.
    IntActi P41835. 1 interaction.
    MINTi MINT-403731.
    STRINGi 4932.YPL214C.

    Proteomic databases

    MaxQBi P41835.
    PaxDbi P41835.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL214C ; YPL214C ; YPL214C .
    GeneIDi 855887.
    KEGGi sce:YPL214C.

    Organism-specific databases

    CYGDi YPL214c.
    SGDi S000006135. THI6.

    Phylogenomic databases

    eggNOGi COG0352.
    HOGENOMi HOG000214306.
    KOi K14154.
    OMAi NLVVQNF.
    OrthoDBi EOG7KDFMZ.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    UPA00060 ; UER00141 .
    BioCyci MetaCyc:YPL214C-MONOMER.
    YEAST:YPL214C-MONOMER.

    Miscellaneous databases

    NextBioi 980553.

    Gene expression databases

    Genevestigatori P41835.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.40.1190.20. 1 hit.
    HAMAPi MF_00097. TMP_synthase.
    InterProi IPR013785. Aldolase_TIM.
    IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    IPR022998. ThiaminP_synth_SF.
    IPR003733. TMP_synthase.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    PF02581. TMP-TENI. 1 hit.
    [Graphical view ]
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF51391. SSF51391. 1 hit.
    SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00693. thiE. 1 hit.
    TIGR00694. thiM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the THI6 gene encoding a bifunctional thiamin-phosphate pyrophosphorylase/hydroxyethylthiazole kinase from Saccharomyces cerevisiae."
      Nosaka K., Nishimura H., Kawasaki Y., Tsujihara T., Iwashima A.
      J. Biol. Chem. 269:30510-30516(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ND5-5A.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiTHI6_YEAST
    AccessioniPrimary (citable) accession number: P41835
    Secondary accession number(s): D6W3F6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3