P41833 (IME4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N6-adenosine-methyltransferase IME4
EC=2.1.1.62

Gene names

Name:	IME4
Synonyms:	SPO8
Ordered Locus Names:	YGL192W
ORF Names:	G1337

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	N6-methyltransferase that methylates adenosine residues of some mRNAs and is essential to activate sporulation. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, is probably required to initiate sporulation. Positive regulator for IME2. Ref.6
Catalytic activity	S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.
Subcellular location	Nucleus Probable.
Induction	In response to starvation conditions. Ref.6
Sequence similarities	Belongs to the MT-A70-like family.

Ontologies

Keywords
Biological process	Meiosis Sporulation
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	meiosis Inferred from mutant phenotype Ref.6Ref.1. Source: SGD sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: EC mRNA (N6-adenosine)-methyltransferase activity Inferred from mutant phenotype Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	N6-adenosine-methyltransferase IME4		PRO_0000207634
Experimental info
	Mutagenesis	348	1	D → A: Impairs ability to sporulate. Ref.6
	Mutagenesis	351	1	W → A: Impairs ability to sporulate. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P41833 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A99BE7EBA76790BA	FASTA	600	69,396
	10 20 30 40 50 60 MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD EVLQFEQFWN 70 80 90 100 110 120 DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY NKFHAFYYTL KSSSSSCEEK 130 140 150 160 170 180 VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK 190 200 210 220 230 240 ERAKYTTEVI EYIPICSDYS HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS 250 260 270 280 290 300 KIHFIPNIKP QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR 310 320 330 340 350 360 SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA WNIHMNLPYG 370 380 390 400 410 420 TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW GYNVINEVSW IKTNQLGRTI 430 440 450 460 470 480 VTGRTGHWLN HSKEHLLVGL KGNPKWINKH IDVDLIVSMT RETSRKPDEL YGIAERLAGT 490 500 510 520 530 540 HARKLEIFGR DHNTRPGWFT IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK 550 560 570 580 590 600 QPSKLQQQHQ QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae." Shah J.C., Clancy M.J. Mol. Cell. Biol. 12:1078-1086(1992) [PubMed: 1545790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GKY5.
[2]	Sakurai M. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene." Clancy M.J., Shambaugh M.E., Timpte C.S., Bokar J.A. Nucleic Acids Res. 30:4509-4518(2002) [PubMed: 12384598] [Abstract] Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF ASP-348 AND TRP-351.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30859 Genomic DNA. Translation: AAA74443.1. D23721 Genomic DNA. Translation: BAA04938.1. X91837 Genomic DNA. Translation: CAA62952.1. Z72714 Genomic DNA. Translation: CAA96904.1. BK006941 Genomic DNA. Translation: DAA07923.1.
PIR	S48509.
RefSeq	NP_011323.1. NM_001181057.1.
3D structure databases
ProteinModelPortal	P41833.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1382N.
IntAct	P41833. 3 interactions.
MINT	MINT-399649.
STRING	P41833.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL192W; YGL192W; YGL192W.
GeneID	852683.
KEGG	sce:YGL192W.
NMPDR	fig|4932.3.peg.2426.
Organism-specific databases
CYGD	YGL192w.
SGD	S000003160. IME4.
Phylogenomic databases
eggNOG	fuNOG07883.
GeneTree	EFGT00070000010637.
HOGENOM	HBG396087.
OrthoDB	EOG476P80.
Gene expression databases
ArrayExpress	P41833.
Genevestigator	P41833.
GermOnline	YGL192W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007757. MT-A70. [Graphical view]
Pfam	PF05063. MT-A70. 1 hit. [Graphical view]
PROSITE	PS51143. MT_A70. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972002.

Entry information

Entry name

IME4_YEAST

Accession

Primary (citable) accession number: P41833
Secondary accession number(s): D6VTW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program