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P41833

- IME4_YEAST

UniProt

P41833 - IME4_YEAST

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Protein

N6-adenosine-methyltransferase IME4

Gene
IME4, SPO8, YGL192W, G1337
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic component of the MIS complex, a complex that mediates N6-methyladenosine (m6A) methylation on some mRNAs during meiosis and is required for sporulation. M6A, which takes place on the adenosine of 5'-[AG]GAC-3' consensus sites of some mRNAs, is probably required to initiate sporulation. Positive regulator for IME2.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

GO - Molecular functioni

  1. mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Source: UniProtKB-EC
  2. mRNA (N6-adenosine)-methyltransferase activity Source: SGD
  3. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. meiotic nuclear division Source: SGD
  2. mRNA methylation Source: SGD
  3. negative regulation of pseudohyphal growth Source: SGD
  4. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Meiosis, Sporulation

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-30673-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N6-adenosine-methyltransferase IME4 (EC:2.1.1.62)
Gene namesi
Name:IME4
Synonyms:SPO8
Ordered Locus Names:YGL192W
ORF Names:G1337
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL192w.
SGDiS000003160. IME4.

Subcellular locationi

Cytoplasm. Nucleusnucleolus
Note: Localizes in the nucleolus during the period of m6A mRNA accumulation.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. MIS complex Source: UniProtKB
  3. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi348 – 3481D → A: Impairs ability to sporulate. 1 Publication
Mutagenesisi351 – 3511W → A: Impairs ability to sporulate. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600N6-adenosine-methyltransferase IME4PRO_0000207634Add
BLAST

Expressioni

Inductioni

In response to starvation conditions.1 Publication

Gene expression databases

GenevestigatoriP41833.

Interactioni

Subunit structurei

Component of the MIS complex, composed of SLZ1, MUM2 and IME4.2 Publications

Protein-protein interaction databases

BioGridi33064. 11 interactions.
DIPiDIP-1382N.
IntActiP41833. 2 interactions.
MINTiMINT-399649.
STRINGi4932.YGL192W.

Structurei

3D structure databases

ProteinModelPortaliP41833.

Family & Domainsi

Sequence similaritiesi

Belongs to the MT-A70-like family.

Phylogenomic databases

eggNOGiCOG4725.
GeneTreeiENSGT00550000075058.
HOGENOMiHOG000247956.
OrthoDBiEOG7KDFNB.

Family and domain databases

InterProiIPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41833-1 [UniParc]FASTAAdd to Basket

« Hide

MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD    50
EVLQFEQFWN DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY 100
NKFHAFYYTL KSSSSSCEEK VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN 150
VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK ERAKYTTEVI EYIPICSDYS 200
HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS KIHFIPNIKP 250
QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR 300
SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA 350
WNIHMNLPYG TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW 400
GYNVINEVSW IKTNQLGRTI VTGRTGHWLN HSKEHLLVGL KGNPKWINKH 450
IDVDLIVSMT RETSRKPDEL YGIAERLAGT HARKLEIFGR DHNTRPGWFT 500
IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK QPSKLQQQHQ 550
QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ 600
Length:600
Mass (Da):69,396
Last modified:November 1, 1995 - v1
Checksum:iA99BE7EBA76790BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U30859 Genomic DNA. Translation: AAA74443.1.
D23721 Genomic DNA. Translation: BAA04938.1.
X91837 Genomic DNA. Translation: CAA62952.1.
Z72714 Genomic DNA. Translation: CAA96904.1.
BK006941 Genomic DNA. Translation: DAA07923.1.
PIRiS48509.
RefSeqiNP_011323.3. NM_001181057.3.

Genome annotation databases

EnsemblFungiiYGL192W; YGL192W; YGL192W.
GeneIDi852683.
KEGGisce:YGL192W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U30859 Genomic DNA. Translation: AAA74443.1 .
D23721 Genomic DNA. Translation: BAA04938.1 .
X91837 Genomic DNA. Translation: CAA62952.1 .
Z72714 Genomic DNA. Translation: CAA96904.1 .
BK006941 Genomic DNA. Translation: DAA07923.1 .
PIRi S48509.
RefSeqi NP_011323.3. NM_001181057.3.

3D structure databases

ProteinModelPortali P41833.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33064. 11 interactions.
DIPi DIP-1382N.
IntActi P41833. 2 interactions.
MINTi MINT-399649.
STRINGi 4932.YGL192W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL192W ; YGL192W ; YGL192W .
GeneIDi 852683.
KEGGi sce:YGL192W.

Organism-specific databases

CYGDi YGL192w.
SGDi S000003160. IME4.

Phylogenomic databases

eggNOGi COG4725.
GeneTreei ENSGT00550000075058.
HOGENOMi HOG000247956.
OrthoDBi EOG7KDFNB.

Enzyme and pathway databases

BioCyci YEAST:G3O-30673-MONOMER.

Miscellaneous databases

NextBioi 972002.
PROi P41833.

Gene expression databases

Genevestigatori P41833.

Family and domain databases

InterProi IPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF05063. MT-A70. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51143. MT_A70. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae."
    Shah J.C., Clancy M.J.
    Mol. Cell. Biol. 12:1078-1086(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GKY5.
  2. Sakurai M.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
    Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
    Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene."
    Clancy M.J., Shambaugh M.E., Timpte C.S., Bokar J.A.
    Nucleic Acids Res. 30:4509-4518(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF ASP-348 AND TRP-351.
  7. "RNA methylation by the MIS complex regulates a cell fate decision in yeast."
    Agarwala S.D., Blitzblau H.G., Hochwagen A., Fink G.R.
    PLoS Genet. 8:E1002732-E1002732(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MIS COMPLEX, FUNCTION.
  8. "High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis."
    Schwartz S., Agarwala S.D., Mumbach M.R., Jovanovic M., Mertins P., Shishkin A., Tabach Y., Mikkelsen T.S., Satija R., Ruvkun G., Carr S.A., Lander E.S., Fink G.R., Regev A.
    Cell 155:1409-1421(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiIME4_YEAST
AccessioniPrimary (citable) accession number: P41833
Secondary accession number(s): D6VTW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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