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P41833 (IME4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N6-adenosine-methyltransferase IME4

EC=2.1.1.62
Gene names
Name:IME4
Synonyms:SPO8
Ordered Locus Names:YGL192W
ORF Names:G1337
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length600 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic component of the MIS complex, a complex that mediates N6-methyladenosine (m6A) methylation on some mRNAs during meiosis and is required for sporulation. M6A, which takes place on the adenosine of 5'-[AG]GAC-3' consensus sites of some mRNAs, is probably required to initiate sporulation. Positive regulator for IME2. Ref.6 Ref.7 Ref.8

Catalytic activity

S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

Subunit structure

Component of the MIS complex, composed of SLZ1, MUM2 and IME4. Ref.7 Ref.8

Subcellular location

Cytoplasm. Nucleusnucleolus. Note: Localizes in the nucleolus during the period of m6A mRNA accumulation. Ref.8

Induction

In response to starvation conditions. Ref.6

Sequence similarities

Belongs to the MT-A70-like family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 600600N6-adenosine-methyltransferase IME4
PRO_0000207634

Experimental info

Mutagenesis3481D → A: Impairs ability to sporulate. Ref.6
Mutagenesis3511W → A: Impairs ability to sporulate. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P41833 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A99BE7EBA76790BA

FASTA60069,396
        10         20         30         40         50         60 
MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD EVLQFEQFWN 

        70         80         90        100        110        120 
DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY NKFHAFYYTL KSSSSSCEEK 

       130        140        150        160        170        180 
VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK 

       190        200        210        220        230        240 
ERAKYTTEVI EYIPICSDYS HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS 

       250        260        270        280        290        300 
KIHFIPNIKP QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR 

       310        320        330        340        350        360 
SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA WNIHMNLPYG 

       370        380        390        400        410        420 
TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW GYNVINEVSW IKTNQLGRTI 

       430        440        450        460        470        480 
VTGRTGHWLN HSKEHLLVGL KGNPKWINKH IDVDLIVSMT RETSRKPDEL YGIAERLAGT 

       490        500        510        520        530        540 
HARKLEIFGR DHNTRPGWFT IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK 

       550        560        570        580        590        600 
QPSKLQQQHQ QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ 

« Hide

References

« Hide 'large scale' references
[1]"IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae."
Shah J.C., Clancy M.J.
Mol. Cell. Biol. 12:1078-1086(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: GKY5.
[2]Sakurai M.
Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene."
Clancy M.J., Shambaugh M.E., Timpte C.S., Bokar J.A.
Nucleic Acids Res. 30:4509-4518(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF ASP-348 AND TRP-351.
[7]"RNA methylation by the MIS complex regulates a cell fate decision in yeast."
Agarwala S.D., Blitzblau H.G., Hochwagen A., Fink G.R.
PLoS Genet. 8:E1002732-E1002732(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MIS COMPLEX, FUNCTION.
[8]"High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis."
Schwartz S., Agarwala S.D., Mumbach M.R., Jovanovic M., Mertins P., Shishkin A., Tabach Y., Mikkelsen T.S., Satija R., Ruvkun G., Carr S.A., Lander E.S., Fink G.R., Regev A.
Cell 155:1409-1421(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U30859 Genomic DNA. Translation: AAA74443.1.
D23721 Genomic DNA. Translation: BAA04938.1.
X91837 Genomic DNA. Translation: CAA62952.1.
Z72714 Genomic DNA. Translation: CAA96904.1.
BK006941 Genomic DNA. Translation: DAA07923.1.
PIRS48509.
RefSeqNP_011323.3. NM_001181057.3.

3D structure databases

ProteinModelPortalP41833.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33064. 11 interactions.
DIPDIP-1382N.
IntActP41833. 2 interactions.
MINTMINT-399649.
STRING4932.YGL192W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL192W; YGL192W; YGL192W.
GeneID852683.
KEGGsce:YGL192W.

Organism-specific databases

CYGDYGL192w.
SGDS000003160. IME4.

Phylogenomic databases

eggNOGCOG4725.
GeneTreeENSGT00550000075058.
HOGENOMHOG000247956.
OrthoDBEOG7KDFNB.

Enzyme and pathway databases

BioCycYEAST:G3O-30673-MONOMER.

Gene expression databases

GenevestigatorP41833.

Family and domain databases

InterProIPR007757. MT-A70-like.
[Graphical view]
PfamPF05063. MT-A70. 1 hit.
[Graphical view]
PROSITEPS51143. MT_A70. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972002.
PROP41833.

Entry information

Entry nameIME4_YEAST
AccessionPrimary (citable) accession number: P41833
Secondary accession number(s): D6VTW2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families