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P41833

- IME4_YEAST

UniProt

P41833 - IME4_YEAST

Protein

N6-adenosine-methyltransferase IME4

Gene

IME4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic component of the MIS complex, a complex that mediates N6-methyladenosine (m6A) methylation on some mRNAs during meiosis and is required for sporulation. M6A, which takes place on the adenosine of 5'-[AG]GAC-3' consensus sites of some mRNAs, is probably required to initiate sporulation. Positive regulator for IME2.3 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

    GO - Molecular functioni

    1. mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Source: UniProtKB-EC
    2. mRNA (N6-adenosine)-methyltransferase activity Source: SGD
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. meiotic nuclear division Source: SGD
    2. mRNA methylation Source: SGD
    3. negative regulation of pseudohyphal growth Source: SGD
    4. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Meiosis, Sporulation

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30673-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N6-adenosine-methyltransferase IME4 (EC:2.1.1.62)
    Gene namesi
    Name:IME4
    Synonyms:SPO8
    Ordered Locus Names:YGL192W
    ORF Names:G1337
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL192w.
    SGDiS000003160. IME4.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleusnucleolus 1 Publication
    Note: Localizes in the nucleolus during the period of m6A mRNA accumulation.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. MIS complex Source: UniProtKB
    3. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi348 – 3481D → A: Impairs ability to sporulate. 1 Publication
    Mutagenesisi351 – 3511W → A: Impairs ability to sporulate. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 600600N6-adenosine-methyltransferase IME4PRO_0000207634Add
    BLAST

    Expressioni

    Inductioni

    In response to starvation conditions.1 Publication

    Gene expression databases

    GenevestigatoriP41833.

    Interactioni

    Subunit structurei

    Component of the MIS complex, composed of SLZ1, MUM2 and IME4.2 Publications

    Protein-protein interaction databases

    BioGridi33064. 11 interactions.
    DIPiDIP-1382N.
    IntActiP41833. 2 interactions.
    MINTiMINT-399649.
    STRINGi4932.YGL192W.

    Structurei

    3D structure databases

    ProteinModelPortaliP41833.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MT-A70-like family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4725.
    GeneTreeiENSGT00550000075058.
    HOGENOMiHOG000247956.
    OrthoDBiEOG7KDFNB.

    Family and domain databases

    InterProiIPR007757. MT-A70-like.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF05063. MT-A70. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51143. MT_A70. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41833-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD    50
    EVLQFEQFWN DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY 100
    NKFHAFYYTL KSSSSSCEEK VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN 150
    VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK ERAKYTTEVI EYIPICSDYS 200
    HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS KIHFIPNIKP 250
    QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR 300
    SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA 350
    WNIHMNLPYG TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW 400
    GYNVINEVSW IKTNQLGRTI VTGRTGHWLN HSKEHLLVGL KGNPKWINKH 450
    IDVDLIVSMT RETSRKPDEL YGIAERLAGT HARKLEIFGR DHNTRPGWFT 500
    IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK QPSKLQQQHQ 550
    QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ 600
    Length:600
    Mass (Da):69,396
    Last modified:November 1, 1995 - v1
    Checksum:iA99BE7EBA76790BA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U30859 Genomic DNA. Translation: AAA74443.1.
    D23721 Genomic DNA. Translation: BAA04938.1.
    X91837 Genomic DNA. Translation: CAA62952.1.
    Z72714 Genomic DNA. Translation: CAA96904.1.
    BK006941 Genomic DNA. Translation: DAA07923.1.
    PIRiS48509.
    RefSeqiNP_011323.3. NM_001181057.3.

    Genome annotation databases

    EnsemblFungiiYGL192W; YGL192W; YGL192W.
    GeneIDi852683.
    KEGGisce:YGL192W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U30859 Genomic DNA. Translation: AAA74443.1 .
    D23721 Genomic DNA. Translation: BAA04938.1 .
    X91837 Genomic DNA. Translation: CAA62952.1 .
    Z72714 Genomic DNA. Translation: CAA96904.1 .
    BK006941 Genomic DNA. Translation: DAA07923.1 .
    PIRi S48509.
    RefSeqi NP_011323.3. NM_001181057.3.

    3D structure databases

    ProteinModelPortali P41833.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33064. 11 interactions.
    DIPi DIP-1382N.
    IntActi P41833. 2 interactions.
    MINTi MINT-399649.
    STRINGi 4932.YGL192W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL192W ; YGL192W ; YGL192W .
    GeneIDi 852683.
    KEGGi sce:YGL192W.

    Organism-specific databases

    CYGDi YGL192w.
    SGDi S000003160. IME4.

    Phylogenomic databases

    eggNOGi COG4725.
    GeneTreei ENSGT00550000075058.
    HOGENOMi HOG000247956.
    OrthoDBi EOG7KDFNB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30673-MONOMER.

    Miscellaneous databases

    NextBioi 972002.
    PROi P41833.

    Gene expression databases

    Genevestigatori P41833.

    Family and domain databases

    InterProi IPR007757. MT-A70-like.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF05063. MT-A70. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51143. MT_A70. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae."
      Shah J.C., Clancy M.J.
      Mol. Cell. Biol. 12:1078-1086(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GKY5.
    2. Sakurai M.
      Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
      Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
      Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene."
      Clancy M.J., Shambaugh M.E., Timpte C.S., Bokar J.A.
      Nucleic Acids Res. 30:4509-4518(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF ASP-348 AND TRP-351.
    7. "RNA methylation by the MIS complex regulates a cell fate decision in yeast."
      Agarwala S.D., Blitzblau H.G., Hochwagen A., Fink G.R.
      PLoS Genet. 8:E1002732-E1002732(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MIS COMPLEX, FUNCTION.
    8. "High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis."
      Schwartz S., Agarwala S.D., Mumbach M.R., Jovanovic M., Mertins P., Shishkin A., Tabach Y., Mikkelsen T.S., Satija R., Ruvkun G., Carr S.A., Lander E.S., Fink G.R., Regev A.
      Cell 155:1409-1421(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiIME4_YEAST
    AccessioniPrimary (citable) accession number: P41833
    Secondary accession number(s): D6VTW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3