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Protein

N6-adenosine-methyltransferase IME4

Gene

IME4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the MIS complex, a complex that mediates N6-methyladenosine (m6A) methylation on some mRNAs during meiosis and is required for sporulation. M6A, which takes place on the adenosine of 5'-[AG]GAC-3' consensus sites of some mRNAs, is probably required to initiate sporulation. Positive regulator for IME2.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

GO - Molecular functioni

  1. mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Source: UniProtKB-EC
  2. mRNA (N6-adenosine)-methyltransferase activity Source: SGD
  3. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. meiotic nuclear division Source: SGD
  2. mRNA methylation Source: SGD
  3. negative regulation of pseudohyphal growth Source: SGD
  4. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Meiosis, Sporulation

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-30673-MONOMER.
ReactomeiREACT_317581. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
N6-adenosine-methyltransferase IME4 (EC:2.1.1.62)
Gene namesi
Name:IME4
Synonyms:SPO8
Ordered Locus Names:YGL192W
ORF Names:G1337
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGL192w.
EuPathDBiFungiDB:YGL192W.
SGDiS000003160. IME4.

Subcellular locationi

  1. Cytoplasm 1 Publication
  2. Nucleusnucleolus 1 Publication

  3. Note: Localizes in the nucleolus during the period of m6A mRNA accumulation.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. MIS complex Source: UniProtKB
  3. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi348 – 3481D → A: Impairs ability to sporulate. 1 Publication
Mutagenesisi351 – 3511W → A: Impairs ability to sporulate. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600N6-adenosine-methyltransferase IME4PRO_0000207634Add
BLAST

Expressioni

Inductioni

In response to starvation conditions.1 Publication

Gene expression databases

GenevestigatoriP41833.

Interactioni

Subunit structurei

Component of the MIS complex, composed of SLZ1, MUM2 and IME4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MUM2P382363EBI-9210,EBI-11619
SLZ1P401672EBI-9210,EBI-29095

Protein-protein interaction databases

BioGridi33064. 11 interactions.
DIPiDIP-1382N.
IntActiP41833. 3 interactions.
MINTiMINT-399649.
STRINGi4932.YGL192W.

Structurei

3D structure databases

ProteinModelPortaliP41833.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MT-A70-like family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4725.
GeneTreeiENSGT00550000075058.
HOGENOMiHOG000247956.
InParanoidiP41833.
KOiK05925.
OrthoDBiEOG7KDFNB.

Family and domain databases

InterProiIPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD
60 70 80 90 100
EVLQFEQFWN DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY
110 120 130 140 150
NKFHAFYYTL KSSSSSCEEK VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN
160 170 180 190 200
VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK ERAKYTTEVI EYIPICSDYS
210 220 230 240 250
HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS KIHFIPNIKP
260 270 280 290 300
QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR
310 320 330 340 350
SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA
360 370 380 390 400
WNIHMNLPYG TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW
410 420 430 440 450
GYNVINEVSW IKTNQLGRTI VTGRTGHWLN HSKEHLLVGL KGNPKWINKH
460 470 480 490 500
IDVDLIVSMT RETSRKPDEL YGIAERLAGT HARKLEIFGR DHNTRPGWFT
510 520 530 540 550
IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK QPSKLQQQHQ
560 570 580 590 600
QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ
Length:600
Mass (Da):69,396
Last modified:November 1, 1995 - v1
Checksum:iA99BE7EBA76790BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30859 Genomic DNA. Translation: AAA74443.1.
D23721 Genomic DNA. Translation: BAA04938.1.
X91837 Genomic DNA. Translation: CAA62952.1.
Z72714 Genomic DNA. Translation: CAA96904.1.
BK006941 Genomic DNA. Translation: DAA07923.1.
PIRiS48509.
RefSeqiNP_011323.3. NM_001181057.3.

Genome annotation databases

EnsemblFungiiYGL192W; YGL192W; YGL192W.
GeneIDi852683.
KEGGisce:YGL192W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30859 Genomic DNA. Translation: AAA74443.1.
D23721 Genomic DNA. Translation: BAA04938.1.
X91837 Genomic DNA. Translation: CAA62952.1.
Z72714 Genomic DNA. Translation: CAA96904.1.
BK006941 Genomic DNA. Translation: DAA07923.1.
PIRiS48509.
RefSeqiNP_011323.3. NM_001181057.3.

3D structure databases

ProteinModelPortaliP41833.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33064. 11 interactions.
DIPiDIP-1382N.
IntActiP41833. 3 interactions.
MINTiMINT-399649.
STRINGi4932.YGL192W.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL192W; YGL192W; YGL192W.
GeneIDi852683.
KEGGisce:YGL192W.

Organism-specific databases

CYGDiYGL192w.
EuPathDBiFungiDB:YGL192W.
SGDiS000003160. IME4.

Phylogenomic databases

eggNOGiCOG4725.
GeneTreeiENSGT00550000075058.
HOGENOMiHOG000247956.
InParanoidiP41833.
KOiK05925.
OrthoDBiEOG7KDFNB.

Enzyme and pathway databases

BioCyciYEAST:G3O-30673-MONOMER.
ReactomeiREACT_317581. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

NextBioi972002.
PROiP41833.

Gene expression databases

GenevestigatoriP41833.

Family and domain databases

InterProiIPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae."
    Shah J.C., Clancy M.J.
    Mol. Cell. Biol. 12:1078-1086(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GKY5.
  2. Sakurai M.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
    Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
    Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene."
    Clancy M.J., Shambaugh M.E., Timpte C.S., Bokar J.A.
    Nucleic Acids Res. 30:4509-4518(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF ASP-348 AND TRP-351.
  7. "RNA methylation by the MIS complex regulates a cell fate decision in yeast."
    Agarwala S.D., Blitzblau H.G., Hochwagen A., Fink G.R.
    PLoS Genet. 8:E1002732-E1002732(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MIS COMPLEX, FUNCTION.
  8. "High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis."
    Schwartz S., Agarwala S.D., Mumbach M.R., Jovanovic M., Mertins P., Shishkin A., Tabach Y., Mikkelsen T.S., Satija R., Ruvkun G., Carr S.A., Lander E.S., Fink G.R., Regev A.
    Cell 155:1409-1421(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiIME4_YEAST
AccessioniPrimary (citable) accession number: P41833
Secondary accession number(s): D6VTW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 29, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.