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Protein

N6-adenosine-methyltransferase IME4

Gene

IME4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the MIS complex, a complex that mediates N6-methyladenosine (m6A) methylation on some mRNAs during meiosis and is required for sporulation. M6A, which takes place on the adenosine of 5'-[AG]GAC-3' consensus sites of some mRNAs, is probably required to initiate sporulation. Positive regulator for IME2.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

GO - Molecular functioni

  • mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Source: GO_Central
  • mRNA (N6-adenosine)-methyltransferase activity Source: SGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • meiotic nuclear division Source: SGD
  • mRNA methylation Source: SGD
  • negative regulation of pseudohyphal growth Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Meiosis, Sporulation

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-30673-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N6-adenosine-methyltransferase IME4 (EC:2.1.1.62)
Gene namesi
Name:IME4
Synonyms:SPO8
Ordered Locus Names:YGL192W
ORF Names:G1337
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL192W.
SGDiS000003160. IME4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • MIS complex Source: UniProtKB
  • nuclear speck Source: GO_Central
  • nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi348D → A: Impairs ability to sporulate. 1 Publication1
Mutagenesisi351W → A: Impairs ability to sporulate. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002076341 – 600N6-adenosine-methyltransferase IME4Add BLAST600

Proteomic databases

PRIDEiP41833.

Expressioni

Inductioni

In response to starvation conditions.1 Publication

Interactioni

Subunit structurei

Component of the MIS complex, composed of SLZ1, MUM2 and IME4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MUM2P382363EBI-9210,EBI-11619
SLZ1P401672EBI-9210,EBI-29095

Protein-protein interaction databases

BioGridi33064. 11 interactors.
DIPiDIP-1382N.
IntActiP41833. 3 interactors.
MINTiMINT-399649.

Structurei

3D structure databases

ProteinModelPortaliP41833.
SMRiP41833.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MT-A70-like family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075058.
HOGENOMiHOG000247956.
InParanoidiP41833.
KOiK05925.
OMAiWIRCDVR.
OrthoDBiEOG092C1MSI.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD
60 70 80 90 100
EVLQFEQFWN DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY
110 120 130 140 150
NKFHAFYYTL KSSSSSCEEK VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN
160 170 180 190 200
VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK ERAKYTTEVI EYIPICSDYS
210 220 230 240 250
HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS KIHFIPNIKP
260 270 280 290 300
QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR
310 320 330 340 350
SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA
360 370 380 390 400
WNIHMNLPYG TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW
410 420 430 440 450
GYNVINEVSW IKTNQLGRTI VTGRTGHWLN HSKEHLLVGL KGNPKWINKH
460 470 480 490 500
IDVDLIVSMT RETSRKPDEL YGIAERLAGT HARKLEIFGR DHNTRPGWFT
510 520 530 540 550
IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK QPSKLQQQHQ
560 570 580 590 600
QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ
Length:600
Mass (Da):69,396
Last modified:November 1, 1995 - v1
Checksum:iA99BE7EBA76790BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30859 Genomic DNA. Translation: AAA74443.1.
D23721 Genomic DNA. Translation: BAA04938.1.
X91837 Genomic DNA. Translation: CAA62952.1.
Z72714 Genomic DNA. Translation: CAA96904.1.
BK006941 Genomic DNA. Translation: DAA07923.1.
PIRiS48509.
RefSeqiNP_011323.3. NM_001181057.3.

Genome annotation databases

EnsemblFungiiYGL192W; YGL192W; YGL192W.
GeneIDi852683.
KEGGisce:YGL192W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30859 Genomic DNA. Translation: AAA74443.1.
D23721 Genomic DNA. Translation: BAA04938.1.
X91837 Genomic DNA. Translation: CAA62952.1.
Z72714 Genomic DNA. Translation: CAA96904.1.
BK006941 Genomic DNA. Translation: DAA07923.1.
PIRiS48509.
RefSeqiNP_011323.3. NM_001181057.3.

3D structure databases

ProteinModelPortaliP41833.
SMRiP41833.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33064. 11 interactors.
DIPiDIP-1382N.
IntActiP41833. 3 interactors.
MINTiMINT-399649.

Proteomic databases

PRIDEiP41833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL192W; YGL192W; YGL192W.
GeneIDi852683.
KEGGisce:YGL192W.

Organism-specific databases

EuPathDBiFungiDB:YGL192W.
SGDiS000003160. IME4.

Phylogenomic databases

GeneTreeiENSGT00550000075058.
HOGENOMiHOG000247956.
InParanoidiP41833.
KOiK05925.
OMAiWIRCDVR.
OrthoDBiEOG092C1MSI.

Enzyme and pathway databases

BioCyciYEAST:G3O-30673-MONOMER.

Miscellaneous databases

PROiP41833.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIME4_YEAST
AccessioniPrimary (citable) accession number: P41833
Secondary accession number(s): D6VTW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.