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Protein

Brefeldin A resistance protein

Gene

bfr1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Confers hyper-resistance to brefeldin A (BFA), an inhibitor of intracellular protein transport. Could serve as an efflux pump of various antibiotics.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi918 – 9258ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-1369062. ABC transporters in lipid homeostasis.
R-SPO-265473. Trafficking of dietary sterols.

Protein family/group databases

TCDBi3.A.1.205.11. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Brefeldin A resistance protein
Gene namesi
Name:bfr1
Synonyms:hba2
ORF Names:SPCC18B5.01c, SPCPJ732.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18B5.01c.
PomBaseiSPCC18B5.01c. bfr1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei539 – 55921HelicalSequence analysisAdd
BLAST
Transmembranei575 – 59521HelicalSequence analysisAdd
BLAST
Transmembranei620 – 64021HelicalSequence analysisAdd
BLAST
Transmembranei649 – 66921HelicalSequence analysisAdd
BLAST
Transmembranei684 – 70421HelicalSequence analysisAdd
BLAST
Transmembranei791 – 81121HelicalSequence analysisAdd
BLAST
Transmembranei1220 – 124021HelicalSequence analysisAdd
BLAST
Transmembranei1255 – 127521HelicalSequence analysisAdd
BLAST
Transmembranei1300 – 132021HelicalSequence analysisAdd
BLAST
Transmembranei1338 – 135821HelicalSequence analysisAdd
BLAST
Transmembranei1367 – 138721HelicalSequence analysisAdd
BLAST
Transmembranei1392 – 141221HelicalSequence analysisAdd
BLAST
Transmembranei1492 – 151221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15301530Brefeldin A resistance proteinPRO_0000093451Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...)Sequence analysis
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence analysis
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence analysis
Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence analysis
Glycosylationi450 – 4501N-linked (GlcNAc...)Sequence analysis
Modified residuei486 – 4861Phosphoserine1 Publication
Modified residuei489 – 4891Phosphoserine1 Publication
Modified residuei491 – 4911Phosphothreonine1 Publication
Glycosylationi1159 – 11591N-linked (GlcNAc...)Sequence analysis
Glycosylationi1175 – 11751N-linked (GlcNAc...)Sequence analysis
Modified residuei1186 – 11861Phosphothreonine1 Publication
Glycosylationi1449 – 14491N-linked (GlcNAc...)Sequence analysis
Glycosylationi1460 – 14601N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP41820.

PTM databases

iPTMnetiP41820.

Interactioni

Protein-protein interaction databases

BioGridi275766. 4 interactions.
MINTiMINT-4690107.

Structurei

3D structure databases

ProteinModelPortaliP41820.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini153 – 410258ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini882 – 1125244ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000162078.
InParanoidiP41820.
KOiK08712.
OrthoDBiEOG092C1HKF.
PhylomeDBiP41820.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR029481. ABC_trans_N.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005285. Drug-R_PDR/CDR.
IPR027417. P-loop_NTPase.
IPR010929. PDR_CDR_ABC.
[Graphical view]
PfamiPF01061. ABC2_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
PF14510. ABC_trans_N. 1 hit.
PF06422. PDR_CDR. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00956. 3a01205. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQNSDTTHG QALGSTLNHT TEVTRISNSS DHFEDSSSNV DESLDSSNPS
60 70 80 90 100
SNEKASHTNE EYRSKGNQSY VPSSSNEPSP ESSSNSDSSS SDDSSVDRLA
110 120 130 140 150
GDPFELGENF NLKHYLRAYK DSLQRDDIIT RSSGVCMRDH SVYGVGSGYE
160 170 180 190 200
FLKTFPDIFL QPYRAITEKQ VVEKAILSHC HALANAGELV MVLGQPGSGC
210 220 230 240 250
STFLRSVTSD TVHYKRVEGT THYDGIDKAD MKKFFPGDLL YSGENDVHFP
260 270 280 290 300
SLTTAETLDF AAKCRTPNNR PCNLTRQEYV SRERHLIATA FGLTHTFNTK
310 320 330 340 350
VGNDFVRGVS GGERKRVTIS EGFATRPTIA CWDNSTRGLD SSTAFEFVNV
360 370 380 390 400
LRTCANELKM TSFVTAYQAS EKIYKLFDRI CVLYAGRQIY YGPADKAKQY
410 420 430 440 450
FLDMGFDCHP RETTPDFLTA ISDPKARFPR KGFENRVPRT PDEFEQMWRN
460 470 480 490 500
SSVYADLMAE MESYDKRWTE TTPASSEAPE KDNFGSDISA TTKHELYRQS
510 520 530 540 550
AVAEKSKRVK DTSPYTVTFS QQLWYCLARS WERYINDPAY IGSMAFAFLF
560 570 580 590 600
QSLIIGSIFY DMKLNTVDVF SRGGVLFFSI LFCALQSLSE IANMFSQRPI
610 620 630 640 650
IAKHRASALY HPAADVISSL IVDLPFRFIN ISVFSIVLYF LTNLKRTAGG
660 670 680 690 700
FWTYFLFLFI GATCMSAFFR SLAGIMPNVE SASALGGIGV LAIAIYTGYA
710 720 730 740 750
IPNIDVGWWF RWIAYLDPLQ FGFESLMINE FKARQFECSQ LIPYGSGYDN
760 770 780 790 800
YPVANKICPV TSAEPGTDYV DGSTYLYISF NYKTRQLWRN LAIIIGYYAF
810 820 830 840 850
LVFVNIVASE TLNFNDLKGE YLVFRRGHAP DAVKAAVNEG GKPLDLETGQ
860 870 880 890 900
DTQGGDVVKE SPDNEEELNK EYEGIEKGHD IFSWRNLNYD IQIKGEHRRL
910 920 930 940 950
LNGVQGFVVP GKLTALMGES GAGKTTLLNV LAQRVDTGVV TGDMLVNGRG
960 970 980 990 1000
LDSTFQRRTG YVQQQDVHIG ESTVREALRF SAALRQPASV PLSEKYEYVE
1010 1020 1030 1040 1050
SVIKLLEMES YAEAIIGTPG SGLNVEQRKR ATIGVELAAK PALLLFLDEP
1060 1070 1080 1090 1100
TSGLDSQSAW SIVCFLRKLA DAGQAILCTI HQPSAVLFDQ FDRLLLLQKG
1110 1120 1130 1140 1150
GKTVYFGDIG EHSKTLLNYF ESHGAVHCPD DGNPAEYILD VIGAGATATT
1160 1170 1180 1190 1200
NRDWHEVWNN SEERKAISAE LDKINASFSN SEDKKTLSKE DRSTYAMPLW
1210 1220 1230 1240 1250
FQVKMVMTRN FQSYWREPSI LMSKLALDIF AGLFIGFTFY NQGLGVQNIQ
1260 1270 1280 1290 1300
NKLFAVFMAT VLAVPLINGL QPKFIELRNV FEVREKPSNI YSWVAFVFSA
1310 1320 1330 1340 1350
IIVEIPFNLV FGTLFFLCWF YPIKFYKHIH HPGDKTGYAW LLYMFFQMYF
1360 1370 1380 1390 1400
STFGQAVASA CPNAQTASVV NSLLFTFVIT FNGVLQPNSN LVGFWHWMHS
1410 1420 1430 1440 1450
LTPFTYLIEG LLSDLVHGLP VECKSHEMLT INPPSGQTCG EYMSAFLTNN
1460 1470 1480 1490 1500
TAAGNLLNPN ATTSCSYCPY QTADQFLERF SMRYTHRWRN LGIFVGYVFF
1510 1520 1530
NIFAVLLLFY VFRVMKLRST WLGKKITGTG
Length:1,530
Mass (Da):171,751
Last modified:November 1, 1995 - v1
Checksum:iAD38B029AD14F8AB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti245 – 2451N → H in BAA19929 (PubMed:7883711).Curated
Sequence conflicti245 – 2451N → H in AAB33744 (PubMed:7883711).Curated
Sequence conflicti478 – 4781A → D in AAB33744 (PubMed:7883711).Curated
Sequence conflicti830 – 8301P → A in AAB33744 (PubMed:7883711).Curated
Sequence conflicti997 – 9971E → G in BAA19929 (PubMed:7883711).Curated
Sequence conflicti997 – 9971E → G in AAB33744 (PubMed:7883711).Curated
Sequence conflicti1060 – 10601W → C in AAB33744 (PubMed:7883711).Curated
Sequence conflicti1075 – 10751A → E in AAB33744 (PubMed:7883711).Curated
Sequence conflicti1079 – 10791T → P in AAB33744 (PubMed:7883711).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003671 Genomic DNA. Translation: BAA19929.1.
X82891 Genomic DNA. Translation: CAA58062.1.
S76267 Genomic DNA. Translation: AAB33744.1.
CU329672 Genomic DNA. Translation: CAC34990.1.
PIRiS52239.
T52010.
RefSeqiNP_587932.3. NM_001022923.3.

Genome annotation databases

EnsemblFungiiSPCC18B5.01c.1; SPCC18B5.01c.1:pep; SPCC18B5.01c.
GeneIDi2539195.
KEGGispo:SPCC18B5.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003671 Genomic DNA. Translation: BAA19929.1.
X82891 Genomic DNA. Translation: CAA58062.1.
S76267 Genomic DNA. Translation: AAB33744.1.
CU329672 Genomic DNA. Translation: CAC34990.1.
PIRiS52239.
T52010.
RefSeqiNP_587932.3. NM_001022923.3.

3D structure databases

ProteinModelPortaliP41820.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275766. 4 interactions.
MINTiMINT-4690107.

Protein family/group databases

TCDBi3.A.1.205.11. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiP41820.

Proteomic databases

MaxQBiP41820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC18B5.01c.1; SPCC18B5.01c.1:pep; SPCC18B5.01c.
GeneIDi2539195.
KEGGispo:SPCC18B5.01c.

Organism-specific databases

EuPathDBiFungiDB:SPCC18B5.01c.
PomBaseiSPCC18B5.01c. bfr1.

Phylogenomic databases

HOGENOMiHOG000162078.
InParanoidiP41820.
KOiK08712.
OrthoDBiEOG092C1HKF.
PhylomeDBiP41820.

Enzyme and pathway databases

ReactomeiR-SPO-1369062. ABC transporters in lipid homeostasis.
R-SPO-265473. Trafficking of dietary sterols.

Miscellaneous databases

PROiP41820.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR029481. ABC_trans_N.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005285. Drug-R_PDR/CDR.
IPR027417. P-loop_NTPase.
IPR010929. PDR_CDR_ABC.
[Graphical view]
PfamiPF01061. ABC2_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
PF14510. ABC_trans_N. 1 hit.
PF06422. PDR_CDR. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00956. 3a01205. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBFR1_SCHPO
AccessioniPrimary (citable) accession number: P41820
Secondary accession number(s): O00035
, P87254, Q09194, Q9C0U4, Q9USL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.