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Protein

Brefeldin A resistance protein

Gene

bfr1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Confers hyper-resistance to brefeldin A (BFA), an inhibitor of intracellular protein transport. Could serve as an efflux pump of various antibiotics.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi918 – 925ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAntibiotic resistance, Transport
LigandATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.1.205.11 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Brefeldin A resistance protein
Gene namesi
Name:bfr1
Synonyms:hba2
ORF Names:SPCC18B5.01c, SPCPJ732.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18B5.01c
PomBaseiSPCC18B5.01c bfr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei539 – 559HelicalSequence analysisAdd BLAST21
Transmembranei575 – 595HelicalSequence analysisAdd BLAST21
Transmembranei620 – 640HelicalSequence analysisAdd BLAST21
Transmembranei649 – 669HelicalSequence analysisAdd BLAST21
Transmembranei684 – 704HelicalSequence analysisAdd BLAST21
Transmembranei791 – 811HelicalSequence analysisAdd BLAST21
Transmembranei1220 – 1240HelicalSequence analysisAdd BLAST21
Transmembranei1255 – 1275HelicalSequence analysisAdd BLAST21
Transmembranei1300 – 1320HelicalSequence analysisAdd BLAST21
Transmembranei1338 – 1358HelicalSequence analysisAdd BLAST21
Transmembranei1367 – 1387HelicalSequence analysisAdd BLAST21
Transmembranei1392 – 1412HelicalSequence analysisAdd BLAST21
Transmembranei1492 – 1512HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000934511 – 1530Brefeldin A resistance proteinAdd BLAST1530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi67N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi273N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi334N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi450N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei486Phosphoserine1 Publication1
Modified residuei489Phosphoserine1 Publication1
Modified residuei491Phosphothreonine1 Publication1
Glycosylationi1159N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1175N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1186Phosphothreonine1 Publication1
Glycosylationi1449N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1460N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP41820
PaxDbiP41820
PRIDEiP41820

PTM databases

iPTMnetiP41820

Interactioni

Protein-protein interaction databases

BioGridi275766, 5 interactors
STRINGi4896.SPCC18B5.01c.1

Structurei

3D structure databases

ProteinModelPortaliP41820
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 410ABC transporter 1PROSITE-ProRule annotationAdd BLAST258
Domaini882 – 1125ABC transporter 2PROSITE-ProRule annotationAdd BLAST244

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000162078
InParanoidiP41820
KOiK08712
OMAiWMYWLTP
OrthoDBiEOG092C1HKF
PhylomeDBiP41820

Family and domain databases

CDDicd03233 ABCG_PDR_domain1, 1 hit
cd03232 ABCG_PDR_domain2, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR013525 ABC_2_trans
IPR029481 ABC_trans_N
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR034001 ABCG_PDR_1
IPR034003 ABCG_PDR_2
IPR005285 Drug-R_PDR/CDR
IPR027417 P-loop_NTPase
IPR010929 PDR_CDR_ABC
PfamiView protein in Pfam
PF01061 ABC2_membrane, 2 hits
PF00005 ABC_tran, 2 hits
PF14510 ABC_trans_N, 1 hit
PF06422 PDR_CDR, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00956 3a01205, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 2 hits

Sequencei

Sequence statusi: Complete.

P41820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQNSDTTHG QALGSTLNHT TEVTRISNSS DHFEDSSSNV DESLDSSNPS
60 70 80 90 100
SNEKASHTNE EYRSKGNQSY VPSSSNEPSP ESSSNSDSSS SDDSSVDRLA
110 120 130 140 150
GDPFELGENF NLKHYLRAYK DSLQRDDIIT RSSGVCMRDH SVYGVGSGYE
160 170 180 190 200
FLKTFPDIFL QPYRAITEKQ VVEKAILSHC HALANAGELV MVLGQPGSGC
210 220 230 240 250
STFLRSVTSD TVHYKRVEGT THYDGIDKAD MKKFFPGDLL YSGENDVHFP
260 270 280 290 300
SLTTAETLDF AAKCRTPNNR PCNLTRQEYV SRERHLIATA FGLTHTFNTK
310 320 330 340 350
VGNDFVRGVS GGERKRVTIS EGFATRPTIA CWDNSTRGLD SSTAFEFVNV
360 370 380 390 400
LRTCANELKM TSFVTAYQAS EKIYKLFDRI CVLYAGRQIY YGPADKAKQY
410 420 430 440 450
FLDMGFDCHP RETTPDFLTA ISDPKARFPR KGFENRVPRT PDEFEQMWRN
460 470 480 490 500
SSVYADLMAE MESYDKRWTE TTPASSEAPE KDNFGSDISA TTKHELYRQS
510 520 530 540 550
AVAEKSKRVK DTSPYTVTFS QQLWYCLARS WERYINDPAY IGSMAFAFLF
560 570 580 590 600
QSLIIGSIFY DMKLNTVDVF SRGGVLFFSI LFCALQSLSE IANMFSQRPI
610 620 630 640 650
IAKHRASALY HPAADVISSL IVDLPFRFIN ISVFSIVLYF LTNLKRTAGG
660 670 680 690 700
FWTYFLFLFI GATCMSAFFR SLAGIMPNVE SASALGGIGV LAIAIYTGYA
710 720 730 740 750
IPNIDVGWWF RWIAYLDPLQ FGFESLMINE FKARQFECSQ LIPYGSGYDN
760 770 780 790 800
YPVANKICPV TSAEPGTDYV DGSTYLYISF NYKTRQLWRN LAIIIGYYAF
810 820 830 840 850
LVFVNIVASE TLNFNDLKGE YLVFRRGHAP DAVKAAVNEG GKPLDLETGQ
860 870 880 890 900
DTQGGDVVKE SPDNEEELNK EYEGIEKGHD IFSWRNLNYD IQIKGEHRRL
910 920 930 940 950
LNGVQGFVVP GKLTALMGES GAGKTTLLNV LAQRVDTGVV TGDMLVNGRG
960 970 980 990 1000
LDSTFQRRTG YVQQQDVHIG ESTVREALRF SAALRQPASV PLSEKYEYVE
1010 1020 1030 1040 1050
SVIKLLEMES YAEAIIGTPG SGLNVEQRKR ATIGVELAAK PALLLFLDEP
1060 1070 1080 1090 1100
TSGLDSQSAW SIVCFLRKLA DAGQAILCTI HQPSAVLFDQ FDRLLLLQKG
1110 1120 1130 1140 1150
GKTVYFGDIG EHSKTLLNYF ESHGAVHCPD DGNPAEYILD VIGAGATATT
1160 1170 1180 1190 1200
NRDWHEVWNN SEERKAISAE LDKINASFSN SEDKKTLSKE DRSTYAMPLW
1210 1220 1230 1240 1250
FQVKMVMTRN FQSYWREPSI LMSKLALDIF AGLFIGFTFY NQGLGVQNIQ
1260 1270 1280 1290 1300
NKLFAVFMAT VLAVPLINGL QPKFIELRNV FEVREKPSNI YSWVAFVFSA
1310 1320 1330 1340 1350
IIVEIPFNLV FGTLFFLCWF YPIKFYKHIH HPGDKTGYAW LLYMFFQMYF
1360 1370 1380 1390 1400
STFGQAVASA CPNAQTASVV NSLLFTFVIT FNGVLQPNSN LVGFWHWMHS
1410 1420 1430 1440 1450
LTPFTYLIEG LLSDLVHGLP VECKSHEMLT INPPSGQTCG EYMSAFLTNN
1460 1470 1480 1490 1500
TAAGNLLNPN ATTSCSYCPY QTADQFLERF SMRYTHRWRN LGIFVGYVFF
1510 1520 1530
NIFAVLLLFY VFRVMKLRST WLGKKITGTG
Length:1,530
Mass (Da):171,751
Last modified:November 1, 1995 - v1
Checksum:iAD38B029AD14F8AB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245N → H in BAA19929 (PubMed:7883711).Curated1
Sequence conflicti245N → H in AAB33744 (PubMed:7883711).Curated1
Sequence conflicti478A → D in AAB33744 (PubMed:7883711).Curated1
Sequence conflicti830P → A in AAB33744 (PubMed:7883711).Curated1
Sequence conflicti997E → G in BAA19929 (PubMed:7883711).Curated1
Sequence conflicti997E → G in AAB33744 (PubMed:7883711).Curated1
Sequence conflicti1060W → C in AAB33744 (PubMed:7883711).Curated1
Sequence conflicti1075A → E in AAB33744 (PubMed:7883711).Curated1
Sequence conflicti1079T → P in AAB33744 (PubMed:7883711).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003671 Genomic DNA Translation: BAA19929.1
X82891 Genomic DNA Translation: CAA58062.1
S76267 Genomic DNA Translation: AAB33744.1
CU329672 Genomic DNA Translation: CAC34990.1
PIRiS52239
T52010
RefSeqiNP_587932.3, NM_001022923.3

Genome annotation databases

EnsemblFungiiSPCC18B5.01c.1; SPCC18B5.01c.1:pep; SPCC18B5.01c
GeneIDi2539195
KEGGispo:SPCC18B5.01c

Similar proteinsi

Entry informationi

Entry nameiBFR1_SCHPO
AccessioniPrimary (citable) accession number: P41820
Secondary accession number(s): O00035
, P87254, Q09194, Q9C0U4, Q9USL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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