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Protein

Dimethyladenosine transferase

Gene

DIM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1779)/adenine(1780) in 18S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1779)/N(6)-dimethyladenine(1780) in 18S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei39 – 391S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei64 – 641S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei85 – 851S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei113 – 1131S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei128 – 1281S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • rRNA modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-34149-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase (EC:2.1.1.183)
Alternative name(s):
18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase
18S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene namesi
Name:DIM1
Ordered Locus Names:YPL266W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL266W.
SGDiS000006187. DIM1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: GO_Central
  • nucleolus Source: UniProtKB-SubCell
  • preribosome, small subunit precursor Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Dimethyladenosine transferasePRO_0000101464Add
BLAST

Proteomic databases

MaxQBiP41819.

PTM databases

iPTMnetiP41819.

Interactioni

Protein-protein interaction databases

BioGridi35947. 51 interactions.
DIPiDIP-3924N.
IntActiP41819. 19 interactions.
MINTiMINT-477008.

Structurei

3D structure databases

ProteinModelPortaliP41819.
SMRiP41819. Positions 36-318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 1614Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063389.
HOGENOMiHOG000227963.
InParanoidiP41819.
KOiK14191.
OMAiKNPRPQI.
OrthoDBiEOG092C481U.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41819-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKAAKKKYS GATSSKQVSA EKHLSSVFKF NTDLGQHILK NPLVAQGIVD
60 70 80 90 100
KAQIRPSDVV LEVGPGTGNL TVRILEQAKN VVAVEMDPRM AAELTKRVRG
110 120 130 140 150
TPVEKKLEIM LGDFMKTELP YFDICISNTP YQISSPLVFK LINQPRPPRV
160 170 180 190 200
SILMFQREFA LRLLARPGDS LYCRLSANVQ MWANVTHIMK VGKNNFRPPP
210 220 230 240 250
QVESSVVRLE IKNPRPQVDY NEWDGLLRIV FVRKNRTISA GFKSTTVMDI
260 270 280 290 300
LEKNYKTFLA MNNEMVDDTK GSMHDVVKEK IDTVLKETDL GDKRAGKCDQ
310
NDFLRLLYAF HQVGIHFS
Length:318
Mass (Da):35,951
Last modified:November 1, 1995 - v1
Checksum:iEE77C9DF574A042A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26480 Genomic DNA. Translation: AAA57357.1.
Z73622 Genomic DNA. Translation: CAA98001.1.
BK006949 Genomic DNA. Translation: DAA11170.1.
PIRiS47985.
RefSeqiNP_015057.1. NM_001184080.1.

Genome annotation databases

EnsemblFungiiYPL266W; YPL266W; YPL266W.
GeneIDi855862.
KEGGisce:YPL266W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26480 Genomic DNA. Translation: AAA57357.1.
Z73622 Genomic DNA. Translation: CAA98001.1.
BK006949 Genomic DNA. Translation: DAA11170.1.
PIRiS47985.
RefSeqiNP_015057.1. NM_001184080.1.

3D structure databases

ProteinModelPortaliP41819.
SMRiP41819. Positions 36-318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35947. 51 interactions.
DIPiDIP-3924N.
IntActiP41819. 19 interactions.
MINTiMINT-477008.

PTM databases

iPTMnetiP41819.

Proteomic databases

MaxQBiP41819.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL266W; YPL266W; YPL266W.
GeneIDi855862.
KEGGisce:YPL266W.

Organism-specific databases

EuPathDBiFungiDB:YPL266W.
SGDiS000006187. DIM1.

Phylogenomic databases

GeneTreeiENSGT00530000063389.
HOGENOMiHOG000227963.
InParanoidiP41819.
KOiK14191.
OMAiKNPRPQI.
OrthoDBiEOG092C481U.

Enzyme and pathway databases

BioCyciYEAST:G3O-34149-MONOMER.

Miscellaneous databases

PROiP41819.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDIM1_YEAST
AccessioniPrimary (citable) accession number: P41819
Secondary accession number(s): D6W3A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.