P41818 (GLC8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein GLC8

Gene names

Name:	GLC8
Ordered Locus Names:	YMR311C
ORF Names:	YM9924.03C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulator of GLC7 type-1 protein phosphatase.
Post-translational modification	Phosphorylated by the cyclin-CDKs PCL6-PHO85 and PCL7-PHO85. Phosphorylation of Thr-118 inactivates GLC8. Ref.5
Miscellaneous	Present with 3440 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromosome segregation Inferred from mutant phenotype PubMed 7565759. Source: SGD glycogen biosynthetic process Traceable author statement PubMed 9584086. Source: SGD regulation of phosphoprotein phosphatase activity Inferred from electronic annotation. Source: InterPro regulation of signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay PubMed 11914276PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	enzyme activator activity Inferred from direct assay PubMed 12127071. Source: SGD protein phosphatase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GLC7	P32598	8	EBI-7629,EBI-13715

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Protein GLC8		PRO_0000071517
Amino acid modifications
	Modified residue	12	1	Phosphoserine Ref.8 Ref.9
	Modified residue	118	1	Phosphothreonine; by PHO85 Ref.5
	Modified residue	158	1	Phosphoserine Ref.7 Ref.9
	Modified residue	184	1	Phosphoserine Ref.8 Ref.9
	Modified residue	224	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P41818 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D4017CA8B3B089C2	FASTA	229	26,649
	10 20 30 40 50 60 MGGILKNPLA LSPEQLAQQD PETLEEFRRQ VYENTQKNAK LTSHKRNIPG LDNTKEEGEI 70 80 90 100 110 120 IGTSSTFLPK DTLSLKHEQD MLAKMTPEER VQWNQRNLAE NEITKKQFQD IHIDEPKTPY 130 140 150 160 170 180 QGAVDPHGEY YRVDDDEDED NSDKKPCQVA NDDIDDLSLG EPEFEIKENK QPDFETNDEN 190 200 210 220 DEDSPEARHK KFEEMRKKHY DVRAIFNKKS REALKDEDED EDDSTTKEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae." Cannon J.F., Pringle J.R., Fiechter A., Khalil M. Genetics 136:485-503(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo." Tan Y.S.H., Morcos P.A., Cannon J.F. J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-118.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-184 AND SER-224, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-158 AND SER-184, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L22000 Genomic DNA. Translation: AAA53673.1. Z54141 Genomic DNA. Translation: CAA90829.1. AY557977 Genomic DNA. Translation: AAS56303.1. BK006946 Genomic DNA. Translation: DAA10212.1.
PIR	S47859.
RefSeq	NP_014042.1. NM_001182822.1.
3D structure databases
ProteinModelPortal	P41818.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2349N.
IntAct	P41818. 3 interactions.
MINT	MINT-674661.
STRING	4932.YMR311C.
Proteomic databases
PaxDb	P41818.
PeptideAtlas	P41818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR311C; YMR311C; YMR311C.
GeneID	855359.
KEGG	sce:YMR311C.
Organism-specific databases
CYGD	YMR311c.
SGD	S000004928. GLC8.
Phylogenomic databases
eggNOG	NOG43787.
HOGENOM	HOG000000770.
KO	K16833.
OMA	VANDDID.
OrthoDB	EOG45XC5P.
Gene expression databases
Genevestigator	P41818.
GermOnline	YMR311C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007062. PPI-2. [Graphical view]
Pfam	PF04979. IPP-2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979127.

Entry information

Entry name

GLC8_YEAST

Accession

Primary (citable) accession number: P41818
Secondary accession number(s): D6W0D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program