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P41818 (GLC8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein GLC8
Gene names
Name:GLC8
Ordered Locus Names:YMR311C
ORF Names:YM9924.03C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Modulator of GLC7 type-1 protein phosphatase.

Post-translational modification

Phosphorylated by the cyclin-CDKs PCL6-PHO85 and PCL7-PHO85. Phosphorylation of Thr-118 inactivates GLC8. Ref.5

Miscellaneous

Present with 3440 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GLC7P325983EBI-7629,EBI-13715

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229Protein GLC8
PRO_0000071517

Amino acid modifications

Modified residue121Phosphoserine Ref.7 Ref.9 Ref.10
Modified residue1181Phosphothreonine; by PHO85 Ref.5
Modified residue1581Phosphoserine Ref.8 Ref.9 Ref.10
Modified residue1841Phosphoserine Ref.9 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P41818 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D4017CA8B3B089C2

FASTA22926,649
        10         20         30         40         50         60 
MGGILKNPLA LSPEQLAQQD PETLEEFRRQ VYENTQKNAK LTSHKRNIPG LDNTKEEGEI 

        70         80         90        100        110        120 
IGTSSTFLPK DTLSLKHEQD MLAKMTPEER VQWNQRNLAE NEITKKQFQD IHIDEPKTPY 

       130        140        150        160        170        180 
QGAVDPHGEY YRVDDDEDED NSDKKPCQVA NDDIDDLSLG EPEFEIKENK QPDFETNDEN 

       190        200        210        220 
DEDSPEARHK KFEEMRKKHY DVRAIFNKKS REALKDEDED EDDSTTKEP 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae."
Cannon J.F., Pringle J.R., Fiechter A., Khalil M.
Genetics 136:485-503(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo."
Tan Y.S.H., Morcos P.A., Cannon J.F.
J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-118.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-158 AND SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-158 AND SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L22000 Genomic DNA. Translation: AAA53673.1.
Z54141 Genomic DNA. Translation: CAA90829.1.
AY557977 Genomic DNA. Translation: AAS56303.1.
BK006946 Genomic DNA. Translation: DAA10212.1.
PIRS47859.
RefSeqNP_014042.1. NM_001182822.1.

3D structure databases

ProteinModelPortalP41818.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35491. 42 interactions.
DIPDIP-2349N.
IntActP41818. 1 interaction.
MINTMINT-674661.
STRING4932.YMR311C.

Proteomic databases

MaxQBP41818.
PaxDbP41818.
PeptideAtlasP41818.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR311C; YMR311C; YMR311C.
GeneID855359.
KEGGsce:YMR311C.

Organism-specific databases

CYGDYMR311c.
SGDS000004928. GLC8.

Phylogenomic databases

eggNOGNOG43787.
HOGENOMHOG000000770.
KOK16833.
OMAENDEDSP.
OrthoDBEOG71P2P8.

Enzyme and pathway databases

BioCycYEAST:G3O-32975-MONOMER.

Gene expression databases

GenevestigatorP41818.

Family and domain databases

InterProIPR007062. PPI-2.
[Graphical view]
PfamPF04979. IPP-2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979127.

Entry information

Entry nameGLC8_YEAST
AccessionPrimary (citable) accession number: P41818
Secondary accession number(s): D6W0D8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD