Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein GLC8

Gene

GLC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Modulator of GLC7 type-1 protein phosphatase.

GO - Molecular functioni

  1. enzyme activator activity Source: SGD
  2. protein phosphatase inhibitor activity Source: InterPro

GO - Biological processi

  1. chromosome segregation Source: SGD
  2. glycogen biosynthetic process Source: SGD
  3. positive regulation of catalytic activity Source: GOC
  4. regulation of phosphoprotein phosphatase activity Source: InterPro
  5. regulation of signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-32975-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GLC8
Gene namesi
Name:GLC8
Ordered Locus Names:YMR311C
ORF Names:YM9924.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR311c.
SGDiS000004928. GLC8.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 229229Protein GLC8PRO_0000071517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine3 Publications
Modified residuei118 – 1181Phosphothreonine; by PHO851 Publication
Modified residuei158 – 1581Phosphoserine3 Publications
Modified residuei184 – 1841Phosphoserine2 Publications

Post-translational modificationi

Phosphorylated by the cyclin-CDKs PCL6-PHO85 and PCL7-PHO85. Phosphorylation of Thr-118 inactivates GLC8.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41818.
PaxDbiP41818.
PeptideAtlasiP41818.

Expressioni

Gene expression databases

GenevestigatoriP41818.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GLC7P325983EBI-7629,EBI-13715

Protein-protein interaction databases

BioGridi35491. 42 interactions.
DIPiDIP-2349N.
IntActiP41818. 1 interaction.
MINTiMINT-674661.
STRINGi4932.YMR311C.

Structurei

3D structure databases

ProteinModelPortaliP41818.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG43787.
HOGENOMiHOG000000770.
InParanoidiP41818.
KOiK16833.
OMAiENDEDSP.
OrthoDBiEOG71P2P8.

Family and domain databases

InterProiIPR007062. PPI-2.
[Graphical view]
PfamiPF04979. IPP-2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGILKNPLA LSPEQLAQQD PETLEEFRRQ VYENTQKNAK LTSHKRNIPG
60 70 80 90 100
LDNTKEEGEI IGTSSTFLPK DTLSLKHEQD MLAKMTPEER VQWNQRNLAE
110 120 130 140 150
NEITKKQFQD IHIDEPKTPY QGAVDPHGEY YRVDDDEDED NSDKKPCQVA
160 170 180 190 200
NDDIDDLSLG EPEFEIKENK QPDFETNDEN DEDSPEARHK KFEEMRKKHY
210 220
DVRAIFNKKS REALKDEDED EDDSTTKEP
Length:229
Mass (Da):26,649
Last modified:November 1, 1995 - v1
Checksum:iD4017CA8B3B089C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22000 Genomic DNA. Translation: AAA53673.1.
Z54141 Genomic DNA. Translation: CAA90829.1.
AY557977 Genomic DNA. Translation: AAS56303.1.
BK006946 Genomic DNA. Translation: DAA10212.1.
PIRiS47859.
RefSeqiNP_014042.1. NM_001182822.1.

Genome annotation databases

EnsemblFungiiYMR311C; YMR311C; YMR311C.
GeneIDi855359.
KEGGisce:YMR311C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22000 Genomic DNA. Translation: AAA53673.1.
Z54141 Genomic DNA. Translation: CAA90829.1.
AY557977 Genomic DNA. Translation: AAS56303.1.
BK006946 Genomic DNA. Translation: DAA10212.1.
PIRiS47859.
RefSeqiNP_014042.1. NM_001182822.1.

3D structure databases

ProteinModelPortaliP41818.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35491. 42 interactions.
DIPiDIP-2349N.
IntActiP41818. 1 interaction.
MINTiMINT-674661.
STRINGi4932.YMR311C.

Proteomic databases

MaxQBiP41818.
PaxDbiP41818.
PeptideAtlasiP41818.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR311C; YMR311C; YMR311C.
GeneIDi855359.
KEGGisce:YMR311C.

Organism-specific databases

CYGDiYMR311c.
SGDiS000004928. GLC8.

Phylogenomic databases

eggNOGiNOG43787.
HOGENOMiHOG000000770.
InParanoidiP41818.
KOiK16833.
OMAiENDEDSP.
OrthoDBiEOG71P2P8.

Enzyme and pathway databases

BioCyciYEAST:G3O-32975-MONOMER.

Miscellaneous databases

NextBioi979127.

Gene expression databases

GenevestigatoriP41818.

Family and domain databases

InterProiIPR007062. PPI-2.
[Graphical view]
PfamiPF04979. IPP-2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae."
    Cannon J.F., Pringle J.R., Fiechter A., Khalil M.
    Genetics 136:485-503(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo."
    Tan Y.S.H., Morcos P.A., Cannon J.F.
    J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-118.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-158 AND SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-158 AND SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLC8_YEAST
AccessioniPrimary (citable) accession number: P41818
Secondary accession number(s): D6W0D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 4, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3440 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.