Reviewed, UniProtKB/Swiss-Prot P41816 (OYE3_YEAST)

Names and origin

Protein names

Recommended name:
    NADPH dehydrogenase 3
    EC=1.6.99.1
Alternative name(s):
    Old yellow enzyme 3

Gene names

Name:	OYE3
Ordered Locus Names:	YPL171C
ORF Names:	P2291

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor.
Cofactor	FMN.
Subunit structure	Homodimer or heterodimer.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.
Mass spectrometry	Molecular mass is 44788 Da from positions 2 - 400. Determined by ESI. Ref.1

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to toxin Inferred from expression pattern. Source: SGD
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Ref.1 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AUS1	Q08409	1	EBI-12734,EBI-35723
ESS1	P22696	1	EBI-12734,EBI-6679
OYE2	Q03558	1	EBI-12734,EBI-12729
RSP5	P39940	1	EBI-12734,EBI-16219
SET2	P46995	1	EBI-12734,EBI-16985
SSM4	P40318	1	EBI-12734,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 400	399	NADPH dehydrogenase 3		PRO_0000194475
Sites
	Active site	197	1	Proton donor By similarity
	Binding site	38	1	FMN By similarity
	Binding site	192	1	FMN By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity
	Binding site	244	1	FMN By similarity
	Binding site	349	1	FMN By similarity
	Binding site	376	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41816-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: CC66CAC4B0748FE1	FASTA	400	44,920
	10 20 30 40 50 60 MPFVKGFEPI SLRDTNLFEP IKIGNTQLAH RAVMPPLTRM RATHPGNIPN KEWAAVYYGQ 70 80 90 100 110 120 RAQRPGTMII TEGTFISPQA GGYDNAPGIW SDEQVAEWKN IFLAIHDCQS FAWVQLWSLG 130 140 150 160 170 180 WASFPDVLAR DGLRYDCASD RVYMNATLQE KAKDANNLEH SLTKDDIKQY IKDYIHAAKN 190 200 210 220 230 240 SIAAGADGVE IHSANGYLLN QFLDPHSNKR TDEYGGTIEN RARFTLEVVD ALIETIGPER 250 260 270 280 290 300 VGLRLSPYGT FNSMSGGAEP GIIAQYSYVL GELEKRAKAG KRLAFVHLVE PRVTDPSLVE 310 320 330 340 350 360 GEGEYSEGTN DFAYSIWKGP IIRAGNYALH PEVVREQVKD PRTLIGYGRF FISNPDLVYR 370 380 390 400 LEEGLPLNKY DRSTFYTMSA EGYTDYPTYE EAVDLGWNKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new old yellow enzyme of Saccharomyces cerevisiae." Niino Y.S., Chakraborty S., Brown B.J., Massey V. J. Biol. Chem. 270:1983-1991(1995) [PubMed: 7836424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MASS SPECTROMETRY. Strain: RZ49-1.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Purnelle B., Coster F., Goffeau A. Yeast 12:1483-1492(1996) [PubMed: 8948103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-181. Strain: ATCC 204511 / S288c / AB972.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L29279 Genomic DNA. Translation: AAA64522.1. Z73526 Genomic DNA. Translation: CAA97877.1. Z73527 Genomic DNA. Translation: CAA97878.1. X96770 Genomic DNA. Translation: CAA65573.1.
PIR	A55569.
RefSeq	NP_015154.1.
3D structure databases
HSSP	HSSP built from PDB template 1OYC based on UniProtKB Q02899.
SMR	P41816. Positions 3-399.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6338N.
IntAct	P41816. 7 interactions.
Proteomic databases
PeptideAtlas	P41816.
Genome annotation databases
Ensembl	YPL171C. Saccharomyces cerevisiae. [Contig view]
GeneID	855932.
GenomeReviews	Gene locus YPL171C in contig U00094_GR.
KEGG	sce:YPL171C.
NMPDR	fig|4932.3.peg.6283.
Organism-specific databases
CYGD	YPL171c.
SGD	S000006092. OYE3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P41816.
OMA	P41816. NIYLMSE.
Enzyme and pathway databases
BRENDA	1.6.99.1. 250.
Gene expression databases
ArrayExpress	P41816.
GermOnline	YPL171C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	980675.

Entry information

Entry name

OYE3_YEAST

Accession

Primary (citable) accession number: P41816

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)