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Protein

NADPH dehydrogenase 3

Gene

OYE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381FMNBy similarity
Binding sitei192 – 1921FMNBy similarity
Binding sitei192 – 1921SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei197 – 1971Proton donorBy similarity
Binding sitei244 – 2441FMNBy similarity
Binding sitei349 – 3491FMNBy similarity
Binding sitei376 – 3761SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciYEAST:YPL171C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH dehydrogenase 3 (EC:1.6.99.1)
Alternative name(s):
Old yellow enzyme 3
Gene namesi
Name:OYE3
Ordered Locus Names:YPL171C
ORF Names:P2291
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL171C.
SGDiS000006092. OYE3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400NADPH dehydrogenase 3PRO_0000194475Add
BLAST

Proteomic databases

PeptideAtlasiP41816.

Interactioni

Subunit structurei

Homodimer or heterodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
OYE2Q035582EBI-12734,EBI-12729

Protein-protein interaction databases

BioGridi36012. 43 interactions.
DIPiDIP-6338N.
IntActiP41816. 6 interactions.
MINTiMINT-4083787.

Structurei

3D structure databases

ProteinModelPortaliP41816.
SMRiP41816. Positions 3-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000116231.
InParanoidiP41816.
KOiK00354.
OrthoDBiEOG7M6DK6.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFVKGFEPI SLRDTNLFEP IKIGNTQLAH RAVMPPLTRM RATHPGNIPN
60 70 80 90 100
KEWAAVYYGQ RAQRPGTMII TEGTFISPQA GGYDNAPGIW SDEQVAEWKN
110 120 130 140 150
IFLAIHDCQS FAWVQLWSLG WASFPDVLAR DGLRYDCASD RVYMNATLQE
160 170 180 190 200
KAKDANNLEH SLTKDDIKQY IKDYIHAAKN SIAAGADGVE IHSANGYLLN
210 220 230 240 250
QFLDPHSNKR TDEYGGTIEN RARFTLEVVD ALIETIGPER VGLRLSPYGT
260 270 280 290 300
FNSMSGGAEP GIIAQYSYVL GELEKRAKAG KRLAFVHLVE PRVTDPSLVE
310 320 330 340 350
GEGEYSEGTN DFAYSIWKGP IIRAGNYALH PEVVREQVKD PRTLIGYGRF
360 370 380 390 400
FISNPDLVYR LEEGLPLNKY DRSTFYTMSA EGYTDYPTYE EAVDLGWNKN
Length:400
Mass (Da):44,920
Last modified:January 23, 2007 - v2
Checksum:iCC66CAC4B0748FE1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781P → S in AAT93245 (PubMed:17322287).Curated

Mass spectrometryi

Molecular mass is 44788 Da from positions 2 - 400. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29279 Genomic DNA. Translation: AAA64522.1.
Z73526 Genomic DNA. Translation: CAA97877.1.
Z73527 Genomic DNA. Translation: CAA97878.1.
AY693226 Genomic DNA. Translation: AAT93245.1.
X96770 Genomic DNA. Translation: CAA65573.1.
BK006949 Genomic DNA. Translation: DAA11263.1.
PIRiA55569.
RefSeqiNP_015154.1. NM_001183985.1.

Genome annotation databases

EnsemblFungiiYPL171C; YPL171C; YPL171C.
GeneIDi855932.
KEGGisce:YPL171C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29279 Genomic DNA. Translation: AAA64522.1.
Z73526 Genomic DNA. Translation: CAA97877.1.
Z73527 Genomic DNA. Translation: CAA97878.1.
AY693226 Genomic DNA. Translation: AAT93245.1.
X96770 Genomic DNA. Translation: CAA65573.1.
BK006949 Genomic DNA. Translation: DAA11263.1.
PIRiA55569.
RefSeqiNP_015154.1. NM_001183985.1.

3D structure databases

ProteinModelPortaliP41816.
SMRiP41816. Positions 3-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36012. 43 interactions.
DIPiDIP-6338N.
IntActiP41816. 6 interactions.
MINTiMINT-4083787.

Proteomic databases

PeptideAtlasiP41816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL171C; YPL171C; YPL171C.
GeneIDi855932.
KEGGisce:YPL171C.

Organism-specific databases

EuPathDBiFungiDB:YPL171C.
SGDiS000006092. OYE3.

Phylogenomic databases

HOGENOMiHOG000116231.
InParanoidiP41816.
KOiK00354.
OrthoDBiEOG7M6DK6.

Enzyme and pathway databases

BioCyciYEAST:YPL171C-MONOMER.

Miscellaneous databases

NextBioi980675.
PROiP41816.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new old yellow enzyme of Saccharomyces cerevisiae."
    Niino Y.S., Chakraborty S., Brown B.J., Massey V.
    J. Biol. Chem. 270:1983-1991(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MASS SPECTROMETRY.
    Strain: RZ49-1.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-181.
    Strain: ATCC 204511 / S288c / AB972.

Entry informationi

Entry nameiOYE3_YEAST
AccessioniPrimary (citable) accession number: P41816
Secondary accession number(s): D6W3J7, E9P937
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.