SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P41814

- TRM6_YEAST

UniProt

P41814 - TRM6_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Gene
GCD10, TIF33, TRM6, YNL062C, N2422
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Binds RNA. Also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.3 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. translation initiation factor activity Source: InterPro

GO - Biological processi

  1. regulation of translational initiation Source: InterPro
  2. tRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16692.
YEAST:G3O-33092-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Alternative name(s):
General control non-derepressible protein 10
Short name:
Protein GCD10
tRNA(m1A58)-methyltransferase subunit TRM6
Short name:
tRNA(m1A58)MTase subunit TRM6
Gene namesi
Name:GCD10
Synonyms:TIF33, TRM6
Ordered Locus Names:YNL062C
ORF Names:N2422
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL062c.
SGDiS000005006. GCD10.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: SGD
  2. tRNA (m1A) methyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
PRO_0000123558Add
BLAST

Proteomic databases

MaxQBiP41814.
PaxDbiP41814.
PeptideAtlasiP41814.

Expressioni

Gene expression databases

GenevestigatoriP41814.

Interactioni

Subunit structurei

tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GDC10 and TRM61/GDC14.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GCD14P469593EBI-8995,EBI-7416

Protein-protein interaction databases

BioGridi35761. 14 interactions.
DIPiDIP-3842N.
IntActiP41814. 2 interactions.
MINTiMINT-533615.
STRINGi4932.YNL062C.

Structurei

3D structure databases

ProteinModelPortaliP41814.

Family & Domainsi

Sequence similaritiesi

Belongs to the TRM6/GCD10 family.

Phylogenomic databases

eggNOGiNOG254601.
GeneTreeiENSGT00390000008327.
HOGENOMiHOG000248840.
KOiK03256.
OMAiGNVHHGG.
OrthoDBiEOG706113.

Family and domain databases

InterProiIPR007316. EIF3_gamma.
IPR017423. tRNA_m1A_mtfrase.
[Graphical view]
PANTHERiPTHR12945. PTHR12945. 1 hit.
PfamiPF04189. Gcd10p. 1 hit.
[Graphical view]
PIRSFiPIRSF038170. tRNA_m1A_mtfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

P41814-1 [UniParc]FASTAAdd to Basket

« Hide

MNALTTIDFN QHVIVRLPSK NYKIVELKPN TSVSLGKFGA FEVNDIIGYP    50
FGLTFEIYYD GEEVSSDENR DSKPKNKIPI GKVRLLSQEI KDVNNDKDDG 100
QSEPPLSIKE KSVSLELSSI DSSATNQNLV NMGSKAQELT VEEIEKMKQE 150
SLSSKEIIDK IIKSHKSFHN KTVYSQEKYV NRKKQKFAKY FTVEYLSSSN 200
LLQFLIDKGD IQRVLDMSQE SMGMLLNLAN IQSEGNYLCM DETGGLLVYF 250
LLERMFGGDN ESKSKGKVIV IHENEHANLD LLKFANYSEK FIKEHVHTIS 300
LLDFFEPPTL QEIQSRFTPL PKEEARALKG GKKNSYYRKL RWYNTQWQIL 350
ELTGEFLYDG LVMATTLHLP TLVPKLAEKI HGSRPIVCYG QFKETLLELA 400
HTLYSDLRFL APSILETRCR PYQSIRGKLH PLMTMKGGGG YLMWCHRVIP 450
APEPVSENAT AADSSEKLAE HGAKKQKI 478
Length:478
Mass (Da):54,389
Last modified:November 1, 1995 - v1
Checksum:i99790A1AACE88609
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83511 Genomic DNA. Translation: CAA58501.1.
U12141 Genomic DNA. Translation: AAA99649.1.
Z71338 Genomic DNA. Translation: CAA95935.1.
BK006947 Genomic DNA. Translation: DAA10484.1.
PIRiS51669.
RefSeqiNP_014337.3. NM_001182900.3.

Genome annotation databases

EnsemblFungiiYNL062C; YNL062C; YNL062C.
GeneIDi855663.
KEGGisce:YNL062C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83511 Genomic DNA. Translation: CAA58501.1 .
U12141 Genomic DNA. Translation: AAA99649.1 .
Z71338 Genomic DNA. Translation: CAA95935.1 .
BK006947 Genomic DNA. Translation: DAA10484.1 .
PIRi S51669.
RefSeqi NP_014337.3. NM_001182900.3.

3D structure databases

ProteinModelPortali P41814.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35761. 14 interactions.
DIPi DIP-3842N.
IntActi P41814. 2 interactions.
MINTi MINT-533615.
STRINGi 4932.YNL062C.

Proteomic databases

MaxQBi P41814.
PaxDbi P41814.
PeptideAtlasi P41814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL062C ; YNL062C ; YNL062C .
GeneIDi 855663.
KEGGi sce:YNL062C.

Organism-specific databases

CYGDi YNL062c.
SGDi S000005006. GCD10.

Phylogenomic databases

eggNOGi NOG254601.
GeneTreei ENSGT00390000008327.
HOGENOMi HOG000248840.
KOi K03256.
OMAi GNVHHGG.
OrthoDBi EOG706113.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16692.
YEAST:G3O-33092-MONOMER.

Miscellaneous databases

NextBioi 979932.
PROi P41814.

Gene expression databases

Genevestigatori P41814.

Family and domain databases

InterProi IPR007316. EIF3_gamma.
IPR017423. tRNA_m1A_mtfrase.
[Graphical view ]
PANTHERi PTHR12945. PTHR12945. 1 hit.
Pfami PF04189. Gcd10p. 1 hit.
[Graphical view ]
PIRSFi PIRSF038170. tRNA_m1A_mtfrase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "GCD10, a translational repressor of GCN4, is the RNA-binding subunit of eukaryotic translation initiation factor-3."
    Garcia-Barrio M.T., Naranda T., Vazquez De Aldana C.R., Cuesta R., Hinnebusch A.G., Hershey J.W., Tamame M.
    Genes Dev. 9:1781-1796(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN TRANSLATIONAL REPRESSION, RNA-BINDING.
    Strain: ATCC 204508 / S288c.
  2. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Bergez P., Doignon F., Crouzet M.
    Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA."
    Anderson J., Phan L., Cuesta R., Carlson B.A., Pak M., Asano K., Bjoerk G.R., Tamame M., Hinnebusch A.G.
    Genes Dev. 12:3650-3662(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE SUBUNIT, SUBUNIT, SUBCELLULAR LOCATION.
  6. "The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae."
    Anderson J., Phan L., Hinnebusch A.G.
    Proc. Natl. Acad. Sci. U.S.A. 97:5173-5178(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE SUBUNIT.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRM6_YEAST
AccessioniPrimary (citable) accession number: P41814
Secondary accession number(s): D6W1B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi