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P41814 (TRM6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Alternative name(s):
General control non-derepressible protein 10
Short name=Protein GCD10
tRNA(m1A58)-methyltransferase subunit TRM6
Short name=tRNA(m1A58)MTase subunit TRM6
Gene names
Name:GCD10
Synonyms:TIF33, TRM6
Ordered Locus Names:YNL062C
ORF Names:N2422
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Binds RNA. Also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency. Ref.1 Ref.6 Ref.7

Subunit structure

tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GDC10 and TRM61/GDC14. Ref.6 Ref.7

Subcellular location

Nucleus Ref.6.

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TRM6/GCD10 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GCD14P469593EBI-8995,EBI-7416

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
PRO_0000123558

Sequences

Sequence LengthMass (Da)Tools
P41814 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 99790A1AACE88609

FASTA47854,389
        10         20         30         40         50         60 
MNALTTIDFN QHVIVRLPSK NYKIVELKPN TSVSLGKFGA FEVNDIIGYP FGLTFEIYYD 

        70         80         90        100        110        120 
GEEVSSDENR DSKPKNKIPI GKVRLLSQEI KDVNNDKDDG QSEPPLSIKE KSVSLELSSI 

       130        140        150        160        170        180 
DSSATNQNLV NMGSKAQELT VEEIEKMKQE SLSSKEIIDK IIKSHKSFHN KTVYSQEKYV 

       190        200        210        220        230        240 
NRKKQKFAKY FTVEYLSSSN LLQFLIDKGD IQRVLDMSQE SMGMLLNLAN IQSEGNYLCM 

       250        260        270        280        290        300 
DETGGLLVYF LLERMFGGDN ESKSKGKVIV IHENEHANLD LLKFANYSEK FIKEHVHTIS 

       310        320        330        340        350        360 
LLDFFEPPTL QEIQSRFTPL PKEEARALKG GKKNSYYRKL RWYNTQWQIL ELTGEFLYDG 

       370        380        390        400        410        420 
LVMATTLHLP TLVPKLAEKI HGSRPIVCYG QFKETLLELA HTLYSDLRFL APSILETRCR 

       430        440        450        460        470 
PYQSIRGKLH PLMTMKGGGG YLMWCHRVIP APEPVSENAT AADSSEKLAE HGAKKQKI 

« Hide

References

« Hide 'large scale' references
[1]"GCD10, a translational repressor of GCN4, is the RNA-binding subunit of eukaryotic translation initiation factor-3."
Garcia-Barrio M.T., Naranda T., Vazquez De Aldana C.R., Cuesta R., Hinnebusch A.G., Hershey J.W., Tamame M.
Genes Dev. 9:1781-1796(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN TRANSLATIONAL REPRESSION, RNA-BINDING.
Strain: ATCC 204508 / S288c.
[2]"The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
Bergez P., Doignon F., Crouzet M.
Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[3]Erratum
Bergez P., Doignon F., Crouzet M.
Yeast 12:297-297(1996) [PubMed] [Europe PMC] [Abstract]
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA."
Anderson J., Phan L., Cuesta R., Carlson B.A., Pak M., Asano K., Bjoerk G.R., Tamame M., Hinnebusch A.G.
Genes Dev. 12:3650-3662(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE SUBUNIT, SUBUNIT, SUBCELLULAR LOCATION.
[7]"The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae."
Anderson J., Phan L., Hinnebusch A.G.
Proc. Natl. Acad. Sci. U.S.A. 97:5173-5178(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE SUBUNIT.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83511 Genomic DNA. Translation: CAA58501.1.
U12141 Genomic DNA. Translation: AAA99649.1.
Z71338 Genomic DNA. Translation: CAA95935.1.
BK006947 Genomic DNA. Translation: DAA10484.1.
PIRS51669.
RefSeqNP_014337.3. NM_001182900.3.

3D structure databases

ProteinModelPortalP41814.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35761. 14 interactions.
DIPDIP-3842N.
IntActP41814. 2 interactions.
MINTMINT-533615.
STRING4932.YNL062C.

Proteomic databases

MaxQBP41814.
PaxDbP41814.
PeptideAtlasP41814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL062C; YNL062C; YNL062C.
GeneID855663.
KEGGsce:YNL062C.

Organism-specific databases

CYGDYNL062c.
SGDS000005006. GCD10.

Phylogenomic databases

eggNOGNOG254601.
GeneTreeENSGT00390000008327.
HOGENOMHOG000248840.
KOK03256.
OMAGNVHHGG.
OrthoDBEOG706113.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16692.
YEAST:G3O-33092-MONOMER.

Gene expression databases

GenevestigatorP41814.

Family and domain databases

InterProIPR007316. EIF3_gamma.
IPR017423. tRNA_m1A_mtfrase.
[Graphical view]
PANTHERPTHR12945. PTHR12945. 1 hit.
PfamPF04189. Gcd10p. 1 hit.
[Graphical view]
PIRSFPIRSF038170. tRNA_m1A_mtfrase. 1 hit.
ProtoNetSearch...

Other

NextBio979932.
PROP41814.

Entry information

Entry nameTRM6_YEAST
AccessionPrimary (citable) accession number: P41814
Secondary accession number(s): D6W1B8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families