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Protein

tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6

Gene

GCD10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Binds RNA. Also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.3 Publications

GO - Molecular functioni

  1. translation initiation factor activity Source: InterPro

GO - Biological processi

  1. regulation of translational initiation Source: InterPro
  2. tRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16692.
YEAST:G3O-33092-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Alternative name(s):
General control non-derepressible protein 10
Short name:
Protein GCD10
tRNA(m1A58)-methyltransferase subunit TRM6
Short name:
tRNA(m1A58)MTase subunit TRM6
Gene namesi
Name:GCD10
Synonyms:TIF33, TRM6
Ordered Locus Names:YNL062C
ORF Names:N2422
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL062c.
SGDiS000005006. GCD10.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: SGD
  2. tRNA (m1A) methyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6PRO_0000123558Add
BLAST

Proteomic databases

MaxQBiP41814.
PaxDbiP41814.
PeptideAtlasiP41814.

Expressioni

Gene expression databases

GenevestigatoriP41814.

Interactioni

Subunit structurei

tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GDC10 and TRM61/GDC14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GCD14P469593EBI-8995,EBI-7416

Protein-protein interaction databases

BioGridi35761. 15 interactions.
DIPiDIP-3842N.
IntActiP41814. 2 interactions.
MINTiMINT-533615.
STRINGi4932.YNL062C.

Structurei

3D structure databases

ProteinModelPortaliP41814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TRM6/GCD10 family.Curated

Phylogenomic databases

eggNOGiNOG254601.
GeneTreeiENSGT00390000008327.
HOGENOMiHOG000248840.
InParanoidiP41814.
KOiK03256.
OMAiGNVHHGG.
OrthoDBiEOG706113.

Family and domain databases

InterProiIPR007316. EIF3_gamma.
IPR017423. tRNA_m1A_mtfrase.
[Graphical view]
PANTHERiPTHR12945. PTHR12945. 1 hit.
PfamiPF04189. Gcd10p. 1 hit.
[Graphical view]
PIRSFiPIRSF038170. tRNA_m1A_mtfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

P41814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALTTIDFN QHVIVRLPSK NYKIVELKPN TSVSLGKFGA FEVNDIIGYP
60 70 80 90 100
FGLTFEIYYD GEEVSSDENR DSKPKNKIPI GKVRLLSQEI KDVNNDKDDG
110 120 130 140 150
QSEPPLSIKE KSVSLELSSI DSSATNQNLV NMGSKAQELT VEEIEKMKQE
160 170 180 190 200
SLSSKEIIDK IIKSHKSFHN KTVYSQEKYV NRKKQKFAKY FTVEYLSSSN
210 220 230 240 250
LLQFLIDKGD IQRVLDMSQE SMGMLLNLAN IQSEGNYLCM DETGGLLVYF
260 270 280 290 300
LLERMFGGDN ESKSKGKVIV IHENEHANLD LLKFANYSEK FIKEHVHTIS
310 320 330 340 350
LLDFFEPPTL QEIQSRFTPL PKEEARALKG GKKNSYYRKL RWYNTQWQIL
360 370 380 390 400
ELTGEFLYDG LVMATTLHLP TLVPKLAEKI HGSRPIVCYG QFKETLLELA
410 420 430 440 450
HTLYSDLRFL APSILETRCR PYQSIRGKLH PLMTMKGGGG YLMWCHRVIP
460 470
APEPVSENAT AADSSEKLAE HGAKKQKI
Length:478
Mass (Da):54,389
Last modified:November 1, 1995 - v1
Checksum:i99790A1AACE88609
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83511 Genomic DNA. Translation: CAA58501.1.
U12141 Genomic DNA. Translation: AAA99649.1.
Z71338 Genomic DNA. Translation: CAA95935.1.
BK006947 Genomic DNA. Translation: DAA10484.1.
PIRiS51669.
RefSeqiNP_014337.3. NM_001182900.3.

Genome annotation databases

EnsemblFungiiYNL062C; YNL062C; YNL062C.
GeneIDi855663.
KEGGisce:YNL062C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83511 Genomic DNA. Translation: CAA58501.1.
U12141 Genomic DNA. Translation: AAA99649.1.
Z71338 Genomic DNA. Translation: CAA95935.1.
BK006947 Genomic DNA. Translation: DAA10484.1.
PIRiS51669.
RefSeqiNP_014337.3. NM_001182900.3.

3D structure databases

ProteinModelPortaliP41814.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35761. 15 interactions.
DIPiDIP-3842N.
IntActiP41814. 2 interactions.
MINTiMINT-533615.
STRINGi4932.YNL062C.

Proteomic databases

MaxQBiP41814.
PaxDbiP41814.
PeptideAtlasiP41814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL062C; YNL062C; YNL062C.
GeneIDi855663.
KEGGisce:YNL062C.

Organism-specific databases

CYGDiYNL062c.
SGDiS000005006. GCD10.

Phylogenomic databases

eggNOGiNOG254601.
GeneTreeiENSGT00390000008327.
HOGENOMiHOG000248840.
InParanoidiP41814.
KOiK03256.
OMAiGNVHHGG.
OrthoDBiEOG706113.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16692.
YEAST:G3O-33092-MONOMER.

Miscellaneous databases

NextBioi979932.
PROiP41814.

Gene expression databases

GenevestigatoriP41814.

Family and domain databases

InterProiIPR007316. EIF3_gamma.
IPR017423. tRNA_m1A_mtfrase.
[Graphical view]
PANTHERiPTHR12945. PTHR12945. 1 hit.
PfamiPF04189. Gcd10p. 1 hit.
[Graphical view]
PIRSFiPIRSF038170. tRNA_m1A_mtfrase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GCD10, a translational repressor of GCN4, is the RNA-binding subunit of eukaryotic translation initiation factor-3."
    Garcia-Barrio M.T., Naranda T., Vazquez De Aldana C.R., Cuesta R., Hinnebusch A.G., Hershey J.W., Tamame M.
    Genes Dev. 9:1781-1796(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN TRANSLATIONAL REPRESSION, RNA-BINDING.
    Strain: ATCC 204508 / S288c.
  2. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Bergez P., Doignon F., Crouzet M.
    Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA."
    Anderson J., Phan L., Cuesta R., Carlson B.A., Pak M., Asano K., Bjoerk G.R., Tamame M., Hinnebusch A.G.
    Genes Dev. 12:3650-3662(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE SUBUNIT, SUBUNIT, SUBCELLULAR LOCATION.
  6. "The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae."
    Anderson J., Phan L., Hinnebusch A.G.
    Proc. Natl. Acad. Sci. U.S.A. 97:5173-5178(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE SUBUNIT.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRM6_YEAST
AccessioniPrimary (citable) accession number: P41814
Secondary accession number(s): D6W1B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.