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Protein

tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6

Gene

GCD10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Binds RNA. Also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.3 Publications

GO - Molecular functioni

GO - Biological processi

  • tRNA methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33092-MONOMER.
YEAST:G3O-33092-MONOMER.
BRENDAi2.1.1.220. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Alternative name(s):
General control non-derepressible protein 10
Short name:
Protein GCD10
tRNA(m1A58)-methyltransferase subunit TRM6
Short name:
tRNA(m1A58)MTase subunit TRM6
Gene namesi
Name:GCD10
Synonyms:TIF33, TRM6
Ordered Locus Names:YNL062C
ORF Names:N2422
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL062C.
SGDiS000005006. GCD10.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • tRNA (m1A) methyltransferase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001235581 – 478tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6Add BLAST478

Proteomic databases

MaxQBiP41814.
PRIDEiP41814.

PTM databases

iPTMnetiP41814.

Interactioni

Subunit structurei

tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GDC10 and TRM61/GDC14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GCD14P469593EBI-8995,EBI-7416

Protein-protein interaction databases

BioGridi35761. 16 interactors.
DIPiDIP-3842N.
IntActiP41814. 2 interactors.
MINTiMINT-533615.

Structurei

Secondary structure

1478
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3 – 5Combined sources3
Beta strandi12 – 16Combined sources5
Beta strandi22 – 26Combined sources5
Beta strandi32 – 34Combined sources3
Helixi36 – 38Combined sources3
Beta strandi40 – 42Combined sources3
Helixi43 – 46Combined sources4
Beta strandi53 – 58Combined sources6
Beta strandi82 – 86Combined sources5
Beta strandi189 – 194Combined sources6
Helixi198 – 208Combined sources11
Turni211 – 216Combined sources6
Helixi219 – 228Combined sources10
Beta strandi236 – 241Combined sources6
Beta strandi243 – 245Combined sources3
Helixi246 – 256Combined sources11
Beta strandi267 – 276Combined sources10
Helixi280 – 284Combined sources5
Helixi289 – 295Combined sources7
Beta strandi296 – 300Combined sources5
Helixi301 – 305Combined sources5
Helixi310 – 314Combined sources5
Helixi330 – 353Combined sources24
Beta strandi361 – 364Combined sources4
Helixi369 – 371Combined sources3
Helixi373 – 376Combined sources4
Helixi377 – 379Combined sources3
Beta strandi385 – 391Combined sources7
Helixi393 – 404Combined sources12
Beta strandi409 – 423Combined sources15
Beta strandi441 – 447Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EQJX-ray2.20A1-478[»]
5ERGX-ray2.20A1-478[»]
ProteinModelPortaliP41814.
SMRiP41814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TRM6/GCD10 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000008327.
HOGENOMiHOG000248840.
InParanoidiP41814.
KOiK03256.
OMAiGNVHHGG.
OrthoDBiEOG092C22ME.

Family and domain databases

InterProiIPR017423. TRM6.
[Graphical view]
PANTHERiPTHR12945. PTHR12945. 2 hits.
PfamiPF04189. Gcd10p. 1 hit.
[Graphical view]
PIRSFiPIRSF038170. tRNA_m1A_mtfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

P41814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALTTIDFN QHVIVRLPSK NYKIVELKPN TSVSLGKFGA FEVNDIIGYP
60 70 80 90 100
FGLTFEIYYD GEEVSSDENR DSKPKNKIPI GKVRLLSQEI KDVNNDKDDG
110 120 130 140 150
QSEPPLSIKE KSVSLELSSI DSSATNQNLV NMGSKAQELT VEEIEKMKQE
160 170 180 190 200
SLSSKEIIDK IIKSHKSFHN KTVYSQEKYV NRKKQKFAKY FTVEYLSSSN
210 220 230 240 250
LLQFLIDKGD IQRVLDMSQE SMGMLLNLAN IQSEGNYLCM DETGGLLVYF
260 270 280 290 300
LLERMFGGDN ESKSKGKVIV IHENEHANLD LLKFANYSEK FIKEHVHTIS
310 320 330 340 350
LLDFFEPPTL QEIQSRFTPL PKEEARALKG GKKNSYYRKL RWYNTQWQIL
360 370 380 390 400
ELTGEFLYDG LVMATTLHLP TLVPKLAEKI HGSRPIVCYG QFKETLLELA
410 420 430 440 450
HTLYSDLRFL APSILETRCR PYQSIRGKLH PLMTMKGGGG YLMWCHRVIP
460 470
APEPVSENAT AADSSEKLAE HGAKKQKI
Length:478
Mass (Da):54,389
Last modified:November 1, 1995 - v1
Checksum:i99790A1AACE88609
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83511 Genomic DNA. Translation: CAA58501.1.
U12141 Genomic DNA. Translation: AAA99649.1.
Z71338 Genomic DNA. Translation: CAA95935.1.
BK006947 Genomic DNA. Translation: DAA10484.1.
PIRiS51669.
RefSeqiNP_014337.3. NM_001182900.3.

Genome annotation databases

EnsemblFungiiYNL062C; YNL062C; YNL062C.
GeneIDi855663.
KEGGisce:YNL062C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83511 Genomic DNA. Translation: CAA58501.1.
U12141 Genomic DNA. Translation: AAA99649.1.
Z71338 Genomic DNA. Translation: CAA95935.1.
BK006947 Genomic DNA. Translation: DAA10484.1.
PIRiS51669.
RefSeqiNP_014337.3. NM_001182900.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EQJX-ray2.20A1-478[»]
5ERGX-ray2.20A1-478[»]
ProteinModelPortaliP41814.
SMRiP41814.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35761. 16 interactors.
DIPiDIP-3842N.
IntActiP41814. 2 interactors.
MINTiMINT-533615.

PTM databases

iPTMnetiP41814.

Proteomic databases

MaxQBiP41814.
PRIDEiP41814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL062C; YNL062C; YNL062C.
GeneIDi855663.
KEGGisce:YNL062C.

Organism-specific databases

EuPathDBiFungiDB:YNL062C.
SGDiS000005006. GCD10.

Phylogenomic databases

GeneTreeiENSGT00390000008327.
HOGENOMiHOG000248840.
InParanoidiP41814.
KOiK03256.
OMAiGNVHHGG.
OrthoDBiEOG092C22ME.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33092-MONOMER.
YEAST:G3O-33092-MONOMER.
BRENDAi2.1.1.220. 984.

Miscellaneous databases

PROiP41814.

Family and domain databases

InterProiIPR017423. TRM6.
[Graphical view]
PANTHERiPTHR12945. PTHR12945. 2 hits.
PfamiPF04189. Gcd10p. 1 hit.
[Graphical view]
PIRSFiPIRSF038170. tRNA_m1A_mtfrase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRM6_YEAST
AccessioniPrimary (citable) accession number: P41814
Secondary accession number(s): D6W1B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.