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P41814

- TRM6_YEAST

UniProt

P41814 - TRM6_YEAST

Protein

tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6

Gene

GCD10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Binds RNA. Also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. translation initiation factor activity Source: InterPro

    GO - Biological processi

    1. regulation of translational initiation Source: InterPro
    2. tRNA methylation Source: SGD

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16692.
    YEAST:G3O-33092-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
    Alternative name(s):
    General control non-derepressible protein 10
    Short name:
    Protein GCD10
    tRNA(m1A58)-methyltransferase subunit TRM6
    Short name:
    tRNA(m1A58)MTase subunit TRM6
    Gene namesi
    Name:GCD10
    Synonyms:TIF33, TRM6
    Ordered Locus Names:YNL062C
    ORF Names:N2422
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL062c.
    SGDiS000005006. GCD10.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: SGD
    2. tRNA (m1A) methyltransferase complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6PRO_0000123558Add
    BLAST

    Proteomic databases

    MaxQBiP41814.
    PaxDbiP41814.
    PeptideAtlasiP41814.

    Expressioni

    Gene expression databases

    GenevestigatoriP41814.

    Interactioni

    Subunit structurei

    tRNA (adenine-N(1)-)-methyltransferase is a heterodimer of TRM6/GDC10 and TRM61/GDC14.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GCD14P469593EBI-8995,EBI-7416

    Protein-protein interaction databases

    BioGridi35761. 14 interactions.
    DIPiDIP-3842N.
    IntActiP41814. 2 interactions.
    MINTiMINT-533615.
    STRINGi4932.YNL062C.

    Structurei

    3D structure databases

    ProteinModelPortaliP41814.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TRM6/GCD10 family.Curated

    Phylogenomic databases

    eggNOGiNOG254601.
    GeneTreeiENSGT00390000008327.
    HOGENOMiHOG000248840.
    KOiK03256.
    OMAiGNVHHGG.
    OrthoDBiEOG706113.

    Family and domain databases

    InterProiIPR007316. EIF3_gamma.
    IPR017423. tRNA_m1A_mtfrase.
    [Graphical view]
    PANTHERiPTHR12945. PTHR12945. 1 hit.
    PfamiPF04189. Gcd10p. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038170. tRNA_m1A_mtfrase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P41814-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNALTTIDFN QHVIVRLPSK NYKIVELKPN TSVSLGKFGA FEVNDIIGYP    50
    FGLTFEIYYD GEEVSSDENR DSKPKNKIPI GKVRLLSQEI KDVNNDKDDG 100
    QSEPPLSIKE KSVSLELSSI DSSATNQNLV NMGSKAQELT VEEIEKMKQE 150
    SLSSKEIIDK IIKSHKSFHN KTVYSQEKYV NRKKQKFAKY FTVEYLSSSN 200
    LLQFLIDKGD IQRVLDMSQE SMGMLLNLAN IQSEGNYLCM DETGGLLVYF 250
    LLERMFGGDN ESKSKGKVIV IHENEHANLD LLKFANYSEK FIKEHVHTIS 300
    LLDFFEPPTL QEIQSRFTPL PKEEARALKG GKKNSYYRKL RWYNTQWQIL 350
    ELTGEFLYDG LVMATTLHLP TLVPKLAEKI HGSRPIVCYG QFKETLLELA 400
    HTLYSDLRFL APSILETRCR PYQSIRGKLH PLMTMKGGGG YLMWCHRVIP 450
    APEPVSENAT AADSSEKLAE HGAKKQKI 478
    Length:478
    Mass (Da):54,389
    Last modified:November 1, 1995 - v1
    Checksum:i99790A1AACE88609
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83511 Genomic DNA. Translation: CAA58501.1.
    U12141 Genomic DNA. Translation: AAA99649.1.
    Z71338 Genomic DNA. Translation: CAA95935.1.
    BK006947 Genomic DNA. Translation: DAA10484.1.
    PIRiS51669.
    RefSeqiNP_014337.3. NM_001182900.3.

    Genome annotation databases

    EnsemblFungiiYNL062C; YNL062C; YNL062C.
    GeneIDi855663.
    KEGGisce:YNL062C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83511 Genomic DNA. Translation: CAA58501.1 .
    U12141 Genomic DNA. Translation: AAA99649.1 .
    Z71338 Genomic DNA. Translation: CAA95935.1 .
    BK006947 Genomic DNA. Translation: DAA10484.1 .
    PIRi S51669.
    RefSeqi NP_014337.3. NM_001182900.3.

    3D structure databases

    ProteinModelPortali P41814.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35761. 14 interactions.
    DIPi DIP-3842N.
    IntActi P41814. 2 interactions.
    MINTi MINT-533615.
    STRINGi 4932.YNL062C.

    Proteomic databases

    MaxQBi P41814.
    PaxDbi P41814.
    PeptideAtlasi P41814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL062C ; YNL062C ; YNL062C .
    GeneIDi 855663.
    KEGGi sce:YNL062C.

    Organism-specific databases

    CYGDi YNL062c.
    SGDi S000005006. GCD10.

    Phylogenomic databases

    eggNOGi NOG254601.
    GeneTreei ENSGT00390000008327.
    HOGENOMi HOG000248840.
    KOi K03256.
    OMAi GNVHHGG.
    OrthoDBi EOG706113.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16692.
    YEAST:G3O-33092-MONOMER.

    Miscellaneous databases

    NextBioi 979932.
    PROi P41814.

    Gene expression databases

    Genevestigatori P41814.

    Family and domain databases

    InterProi IPR007316. EIF3_gamma.
    IPR017423. tRNA_m1A_mtfrase.
    [Graphical view ]
    PANTHERi PTHR12945. PTHR12945. 1 hit.
    Pfami PF04189. Gcd10p. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038170. tRNA_m1A_mtfrase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "GCD10, a translational repressor of GCN4, is the RNA-binding subunit of eukaryotic translation initiation factor-3."
      Garcia-Barrio M.T., Naranda T., Vazquez De Aldana C.R., Cuesta R., Hinnebusch A.G., Hershey J.W., Tamame M.
      Genes Dev. 9:1781-1796(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN TRANSLATIONAL REPRESSION, RNA-BINDING.
      Strain: ATCC 204508 / S288c.
    2. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Bergez P., Doignon F., Crouzet M.
      Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA."
      Anderson J., Phan L., Cuesta R., Carlson B.A., Pak M., Asano K., Bjoerk G.R., Tamame M., Hinnebusch A.G.
      Genes Dev. 12:3650-3662(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE SUBUNIT, SUBUNIT, SUBCELLULAR LOCATION.
    6. "The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae."
      Anderson J., Phan L., Hinnebusch A.G.
      Proc. Natl. Acad. Sci. U.S.A. 97:5173-5178(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TRNA METHYLTRANSFERASE SUBUNIT.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTRM6_YEAST
    AccessioniPrimary (citable) accession number: P41814
    Secondary accession number(s): D6W1B8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2580 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3