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P41813

- FKH2_YEAST

UniProt

P41813 - FKH2_YEAST

Protein

Fork head protein homolog 2

Gene

FKH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi339 – 43092Fork-headPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA replication origin binding Source: SGD
    2. RNA polymerase II transcription factor binding Source: SGD
    3. RNA polymerase II transcription factor binding transcription factor activity Source: SGD
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. chromatin remodeling Source: SGD
    2. negative regulation of chromatin silencing at silent mating-type cassette Source: SGD
    3. negative regulation of pseudohyphal growth Source: SGD
    4. negative regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
    5. negative regulation of transcription involved in G2/M transition of mitotic cell cycle Source: SGD
    6. positive regulation of DNA-dependent DNA replication initiation Source: SGD
    7. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
    8. positive regulation of transcription involved in G2/M transition of mitotic cell cycle Source: SGD
    9. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33098-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fork head protein homolog 2
    Gene namesi
    Name:FKH2
    Ordered Locus Names:YNL068C
    ORF Names:N2403, YNL2403C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL068c.
    SGDiS000005012. FKH2.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 862862Fork head protein homolog 2PRO_0000091904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei708 – 7081Phosphoserine1 Publication
    Modified residuei832 – 8321Phosphoserine2 Publications
    Modified residuei833 – 8331Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP41813.
    PaxDbiP41813.
    PeptideAtlasiP41813.
    PRIDEiP41813.

    Expressioni

    Gene expression databases

    GenevestigatoriP41813.

    Interactioni

    Subunit structurei

    Interacts with NDD1.1 Publication

    Protein-protein interaction databases

    BioGridi35755. 132 interactions.
    DIPiDIP-5944N.
    IntActiP41813. 1 interaction.
    MINTiMINT-673870.
    STRINGi4932.YNL068C.

    Structurei

    3D structure databases

    ProteinModelPortaliP41813.
    SMRiP41813. Positions 340-427.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini83 – 15270FHAPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The FHA domain is necessary for the interaction with NDD1.1 Publication

    Sequence similaritiesi

    Contains 1 FHA domain.PROSITE-ProRule annotation
    Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5025.
    GeneTreeiENSGT00750000117242.
    HOGENOMiHOG000093809.
    OMAiGMKWRIS.
    OrthoDBiEOG7647BS.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.200.20. 1 hit.
    InterProiIPR000253. FHA_dom.
    IPR008984. SMAD_FHA_domain.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00498. FHA. 1 hit.
    PF00250. Fork_head. 1 hit.
    [Graphical view]
    PRINTSiPR00053. FORKHEAD.
    SMARTiSM00339. FH. 1 hit.
    SM00240. FHA. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    PROSITEiPS50006. FHA_DOMAIN. 1 hit.
    PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41813-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSNFNEMN ELNMTQTNYG STKYTAQHHQ GVINAIISSL TAPDQPTTVS    50
    LQYSNDKNMA TEIQAYAKLS GPNWTYYVKD LEVSIGRNTD PLNSALQENS 100
    DGVKNSYRVN IDLGPAKVVS RKHAIIKYNM NIGGWELHIL GRNGAKVNFQ 150
    RTHNGPNNPP IRLSSGTLLD IGGTQMMFIL PDSDPVVAPI CIEHLMPNLI 200
    NMFGLEGNNN PLLRDIIKQS NYAKQRQLTS NQQIKGFKLY GSGGNAPFGS 250
    GANLGPSEQG IFNNNNNSKN KNGYFTSINP NYTASTTTSN TINPQAASPQ 300
    GPPNTIIAAN FVDSYKSSNA YPQALDFTSD LSHDENRNVK PPHSYATMIT 350
    QAILSSPEGV ISLADIYKYI SSNYAYYRFA KSGWQNSIRH NLSLNKAFEK 400
    VPRRPNEPGK GMKWRISESY QQEFLNKWNT GKVGKIRRGS SVARQLQLHM 450
    AKFNSLPMEM DYRLSLNMAQ PPKRQLQSHN VLEPSNNNII EGFVQHVPSK 500
    GNLPASQQSQ PPVSHQNQSQ QPPPQEQRQE IQFTFADTQN RNIALARPIK 550
    TPQLQAPNSN ANLNQNNMKE YKESLHPPAI SISQMNRQSP NNALVSFTNA 600
    CANSKIINNI SDSADKSTNN NGGTKMNLPA ISTSSLDENG NLEPTTTTSS 650
    GNSNSVPQTG TTTSSLAANS LRLSQPYDTL LRSPTKAFHI TAMEAYTPER 700
    GSANRARSPL HSNSNNTNNN GANNSNLQTS GMENKQTGLV LDSNVLKSME 750
    SNNDNRRLTP STSKSQNVKS SPGVWNLLQF SSTNNTPAAD SGGNKRGFSI 800
    NPDIKAKENE NATSEKDSDS NSNDLETKDI NSSPLKNQGG STANAKELIL 850
    DTDGAKISII NN 862
    Length:862
    Mass (Da):94,374
    Last modified:November 1, 1995 - v1
    Checksum:i68A03F5EB7BB5CF3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38850 Genomic DNA. Translation: AAA60939.1.
    X86470 Genomic DNA. Translation: CAA60193.1.
    Z71343 Genomic DNA. Translation: CAA95941.1.
    Z71344 Genomic DNA. Translation: CAA95942.1.
    U12141 Genomic DNA. Translation: AAA99643.1.
    BK006947 Genomic DNA. Translation: DAA10477.1.
    PIRiS53913.
    RefSeqiNP_014331.3. NM_001182906.3.

    Genome annotation databases

    EnsemblFungiiYNL068C; YNL068C; YNL068C.
    GeneIDi855656.
    KEGGisce:YNL068C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38850 Genomic DNA. Translation: AAA60939.1 .
    X86470 Genomic DNA. Translation: CAA60193.1 .
    Z71343 Genomic DNA. Translation: CAA95941.1 .
    Z71344 Genomic DNA. Translation: CAA95942.1 .
    U12141 Genomic DNA. Translation: AAA99643.1 .
    BK006947 Genomic DNA. Translation: DAA10477.1 .
    PIRi S53913.
    RefSeqi NP_014331.3. NM_001182906.3.

    3D structure databases

    ProteinModelPortali P41813.
    SMRi P41813. Positions 340-427.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35755. 132 interactions.
    DIPi DIP-5944N.
    IntActi P41813. 1 interaction.
    MINTi MINT-673870.
    STRINGi 4932.YNL068C.

    Proteomic databases

    MaxQBi P41813.
    PaxDbi P41813.
    PeptideAtlasi P41813.
    PRIDEi P41813.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL068C ; YNL068C ; YNL068C .
    GeneIDi 855656.
    KEGGi sce:YNL068C.

    Organism-specific databases

    CYGDi YNL068c.
    SGDi S000005012. FKH2.

    Phylogenomic databases

    eggNOGi COG5025.
    GeneTreei ENSGT00750000117242.
    HOGENOMi HOG000093809.
    OMAi GMKWRIS.
    OrthoDBi EOG7647BS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33098-MONOMER.

    Miscellaneous databases

    NextBioi 979914.

    Gene expression databases

    Genevestigatori P41813.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.200.20. 1 hit.
    InterProi IPR000253. FHA_dom.
    IPR008984. SMAD_FHA_domain.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00498. FHA. 1 hit.
    PF00250. Fork_head. 1 hit.
    [Graphical view ]
    PRINTSi PR00053. FORKHEAD.
    SMARTi SM00339. FH. 1 hit.
    SM00240. FHA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    PROSITEi PS50006. FHA_DOMAIN. 1 hit.
    PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two fork head homologs in S. cerevisiae."
      Zhu G., Davis T.N.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
      Poehlmann R., Philippsen P.
      Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Bergez P., Doignon F., Crouzet M.
      Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-440.
      Strain: S288c / FY1676.
    6. "Recruitment of Thr 319-phosphorylated Ndd1p to the FHA domain of Fkh2p requires Clb kinase activity: a mechanism for CLB cluster gene activation."
      Reynolds D., Shi B.J., McLean C., Katsis F., Kemp B., Dalton S.
      Genes Dev. 17:1789-1802(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN, INTERACTION WITH NDD1.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-832 AND SER-833, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-708; SER-832 AND SER-833, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFKH2_YEAST
    AccessioniPrimary (citable) accession number: P41813
    Secondary accession number(s): D6W1B1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 656 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3