P41813 (FKH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fork head protein homolog 2

Gene names

Name:	FKH2
Ordered Locus Names:	YNL068C
ORF Names:	N2403, YNL2403C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with NDD1. Ref.7
Subcellular location	Nucleus Potential.
Domain	The FHA domain is necessary for the interaction with NDD1. Ref.7
Miscellaneous	Present with 656 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 FHA domain. Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromatin remodeling Inferred from mutant phenotype PubMed 17283050. Source: SGD mitochondrion organization Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of chromatin silencing at silent mating-type cassette Inferred from mutant phenotype PubMed 10747051. Source: SGD negative regulation of pseudohyphal growth Inferred from mutant phenotype PubMed 10894548. Source: SGD negative regulation of transcription involved in G1 phase of mitotic cell cycle Inferred from genetic interaction PubMed 17898805. Source: SGD negative regulation of transcription involved in G2/M-phase of mitotic cell cycle Inferred from genetic interaction PubMed 10894549. Source: SGD positive regulation of transcription elongation from RNA polymerase II promoter Inferred from mutant phenotype PubMed 12702877. Source: SGD positive regulation of transcription involved in G2/M-phase of mitotic cell cycle Inferred from mutant phenotype PubMed 10899128PubMed 10959837. Source: SGD regulation of sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	nuclear chromatin Inferred from Biological aspect of Ancestor. Source: RefGenome transcription factor complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome RNA polymerase II transcription factor binding Inferred from direct assay PubMed 10899128PubMed 10959837. Source: SGD RNA polymerase II transcription factor binding transcription factor activity Inferred from direct assay PubMed 10959837. Source: SGD double-stranded DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome sequence-specific DNA binding Inferred from direct assay PubMed 19111667PubMed 19158363. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 862	862	Fork head protein homolog 2		PRO_0000091904
Regions
	Domain	83 – 152	70	FHA
	DNA binding	339 – 430	92	Fork-head
Amino acid modifications
	Modified residue	441	1	Phosphoserine Ref.9
	Modified residue	813	1	Phosphothreonine Ref.10
	Modified residue	818	1	Phosphoserine Ref.10
	Modified residue	820	1	Phosphoserine Ref.10
	Modified residue	832	1	Phosphoserine Ref.10 Ref.11
	Modified residue	833	1	Phosphoserine Ref.10 Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P41813 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 68A03F5EB7BB5CF3	FASTA	862	94,374
	10 20 30 40 50 60 MSSSNFNEMN ELNMTQTNYG STKYTAQHHQ GVINAIISSL TAPDQPTTVS LQYSNDKNMA 70 80 90 100 110 120 TEIQAYAKLS GPNWTYYVKD LEVSIGRNTD PLNSALQENS DGVKNSYRVN IDLGPAKVVS 130 140 150 160 170 180 RKHAIIKYNM NIGGWELHIL GRNGAKVNFQ RTHNGPNNPP IRLSSGTLLD IGGTQMMFIL 190 200 210 220 230 240 PDSDPVVAPI CIEHLMPNLI NMFGLEGNNN PLLRDIIKQS NYAKQRQLTS NQQIKGFKLY 250 260 270 280 290 300 GSGGNAPFGS GANLGPSEQG IFNNNNNSKN KNGYFTSINP NYTASTTTSN TINPQAASPQ 310 320 330 340 350 360 GPPNTIIAAN FVDSYKSSNA YPQALDFTSD LSHDENRNVK PPHSYATMIT QAILSSPEGV 370 380 390 400 410 420 ISLADIYKYI SSNYAYYRFA KSGWQNSIRH NLSLNKAFEK VPRRPNEPGK GMKWRISESY 430 440 450 460 470 480 QQEFLNKWNT GKVGKIRRGS SVARQLQLHM AKFNSLPMEM DYRLSLNMAQ PPKRQLQSHN 490 500 510 520 530 540 VLEPSNNNII EGFVQHVPSK GNLPASQQSQ PPVSHQNQSQ QPPPQEQRQE IQFTFADTQN 550 560 570 580 590 600 RNIALARPIK TPQLQAPNSN ANLNQNNMKE YKESLHPPAI SISQMNRQSP NNALVSFTNA 610 620 630 640 650 660 CANSKIINNI SDSADKSTNN NGGTKMNLPA ISTSSLDENG NLEPTTTTSS GNSNSVPQTG 670 680 690 700 710 720 TTTSSLAANS LRLSQPYDTL LRSPTKAFHI TAMEAYTPER GSANRARSPL HSNSNNTNNN 730 740 750 760 770 780 GANNSNLQTS GMENKQTGLV LDSNVLKSME SNNDNRRLTP STSKSQNVKS SPGVWNLLQF 790 800 810 820 830 840 SSTNNTPAAD SGGNKRGFSI NPDIKAKENE NATSEKDSDS NSNDLETKDI NSSPLKNQGG 850 860 STANAKELIL DTDGAKISII NN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two fork head homologs in S. cerevisiae." Zhu G., Davis T.N. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs." Poehlmann R., Philippsen P. Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae." Bergez P., Doignon F., Crouzet M. Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-440. Strain: S288c / FY1676.
[6]	Erratum Bergez P., Doignon F., Crouzet M. Yeast 12:297-297(1996) [PubMed] [Europe PMC] [Abstract]
[7]	"Recruitment of Thr 319-phosphorylated Ndd1p to the FHA domain of Fkh2p requires Clb kinase activity: a mechanism for CLB cluster gene activation." Reynolds D., Shi B.J., McLean C., Katsis F., Kemp B., Dalton S. Genes Dev. 17:1789-1802(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN, INTERACTION WITH NDD1.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-441, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-813; SER-818; SER-820; SER-832 AND SER-833, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-832 AND SER-833, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L38850 Genomic DNA. Translation: AAA60939.1. X86470 Genomic DNA. Translation: CAA60193.1. Z71343 Genomic DNA. Translation: CAA95941.1. Z71344 Genomic DNA. Translation: CAA95942.1. U12141 Genomic DNA. Translation: AAA99643.1. BK006947 Genomic DNA. Translation: DAA10477.1.
PIR	S53913.
RefSeq	NP_014331.3. NM_001182906.3.
3D structure databases
ProteinModelPortal	P41813.
SMR	P41813. Positions 340-427.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5944N.
IntAct	P41813. 4 interactions.
MINT	MINT-673870.
STRING	4932.YNL068C.
Proteomic databases
PaxDb	P41813.
PeptideAtlas	P41813.
PRIDE	P41813.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL068C; YNL068C; YNL068C.
GeneID	855656.
KEGG	sce:YNL062C. sce:YNL068C.
Organism-specific databases
CYGD	YNL068c.
SGD	S000005012. FKH2.
Phylogenomic databases
eggNOG	COG5025.
GeneTree	ENSGT00690000101938.
HOGENOM	HOG000093809.
KO	K03256.
OMA	GMKWRIS.
OrthoDB	EOG4R7ZKG.
Gene expression databases
Genevestigator	P41813.
GermOnline	YNL068C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 2.60.200.20. 1 hit.
InterPro	IPR000253. FHA_dom. IPR008984. SMAD_FHA_domain. IPR001766. TF_fork_head. IPR018122. TF_fork_head_CS. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00498. FHA. 1 hit. PF00250. Fork_head. 1 hit. [Graphical view]
PRINTS	PR00053. FORKHEAD.
SMART	SM00339. FH. 1 hit. SM00240. FHA. 1 hit. [Graphical view]
SUPFAM	SSF49879. SMAD_FHA. 1 hit.
PROSITE	PS50006. FHA_DOMAIN. 1 hit. PS00657. FORK_HEAD_1. 1 hit. PS00658. FORK_HEAD_2. 1 hit. PS50039. FORK_HEAD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979914.

Entry information

Entry name

FKH2_YEAST

Accession

Primary (citable) accession number: P41813
Secondary accession number(s): D6W1B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program