Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P41813 (FKH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fork head protein homolog 2
Gene names
Name:FKH2
Ordered Locus Names:YNL068C
ORF Names:N2403, YNL2403C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length862 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with NDD1. Ref.7

Subcellular location

Nucleus Potential.

Domain

The FHA domain is necessary for the interaction with NDD1. Ref.7

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 FHA domain.

Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
   Cellular componentNucleus
   LigandDNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchromatin remodeling

Inferred from mutant phenotype PubMed 17283050. Source: SGD

negative regulation of chromatin silencing at silent mating-type cassette

Inferred from mutant phenotype PubMed 10747051. Source: SGD

negative regulation of pseudohyphal growth

Inferred from mutant phenotype PubMed 10894548. Source: SGD

negative regulation of transcription involved in G1/S transition of mitotic cell cycle

Inferred from genetic interaction PubMed 17898805. Source: SGD

negative regulation of transcription involved in G2/M transition of mitotic cell cycle

Inferred from genetic interaction PubMed 10894549. Source: SGD

positive regulation of DNA-dependent DNA replication initiation

Inferred from mutant phenotype PubMed 22265405. Source: SGD

positive regulation of transcription elongation from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 12702877. Source: SGD

positive regulation of transcription involved in G2/M transition of mitotic cell cycle

Inferred from mutant phenotype PubMed 10899128PubMed 10959837. Source: SGD

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 10899128PubMed 10959837PubMed 15509804. Source: GOC

   Cellular_componentcytosol

Inferred from direct assay PubMed 22932476. Source: SGD

nucleus

Inferred from direct assay PubMed 10899128PubMed 20847055PubMed 22932476. Source: SGD

   Molecular_functionDNA replication origin binding

Inferred from direct assay PubMed 22265405. Source: SGD

RNA polymerase II transcription factor binding

Inferred from direct assay PubMed 10899128PubMed 10959837PubMed 12711672PubMed 15509804. Source: SGD

RNA polymerase II transcription factor binding transcription factor activity

Inferred from direct assay PubMed 10899128PubMed 10959837PubMed 15509804. Source: SGD

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 862862Fork head protein homolog 2
PRO_0000091904

Regions

Domain83 – 15270FHA
DNA binding339 – 43092Fork-head

Amino acid modifications

Modified residue7081Phosphoserine Ref.11
Modified residue8321Phosphoserine Ref.10 Ref.11
Modified residue8331Phosphoserine Ref.10 Ref.11

Sequences

Sequence LengthMass (Da)Tools
P41813 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 68A03F5EB7BB5CF3

FASTA86294,374
        10         20         30         40         50         60 
MSSSNFNEMN ELNMTQTNYG STKYTAQHHQ GVINAIISSL TAPDQPTTVS LQYSNDKNMA 

        70         80         90        100        110        120 
TEIQAYAKLS GPNWTYYVKD LEVSIGRNTD PLNSALQENS DGVKNSYRVN IDLGPAKVVS 

       130        140        150        160        170        180 
RKHAIIKYNM NIGGWELHIL GRNGAKVNFQ RTHNGPNNPP IRLSSGTLLD IGGTQMMFIL 

       190        200        210        220        230        240 
PDSDPVVAPI CIEHLMPNLI NMFGLEGNNN PLLRDIIKQS NYAKQRQLTS NQQIKGFKLY 

       250        260        270        280        290        300 
GSGGNAPFGS GANLGPSEQG IFNNNNNSKN KNGYFTSINP NYTASTTTSN TINPQAASPQ 

       310        320        330        340        350        360 
GPPNTIIAAN FVDSYKSSNA YPQALDFTSD LSHDENRNVK PPHSYATMIT QAILSSPEGV 

       370        380        390        400        410        420 
ISLADIYKYI SSNYAYYRFA KSGWQNSIRH NLSLNKAFEK VPRRPNEPGK GMKWRISESY 

       430        440        450        460        470        480 
QQEFLNKWNT GKVGKIRRGS SVARQLQLHM AKFNSLPMEM DYRLSLNMAQ PPKRQLQSHN 

       490        500        510        520        530        540 
VLEPSNNNII EGFVQHVPSK GNLPASQQSQ PPVSHQNQSQ QPPPQEQRQE IQFTFADTQN 

       550        560        570        580        590        600 
RNIALARPIK TPQLQAPNSN ANLNQNNMKE YKESLHPPAI SISQMNRQSP NNALVSFTNA 

       610        620        630        640        650        660 
CANSKIINNI SDSADKSTNN NGGTKMNLPA ISTSSLDENG NLEPTTTTSS GNSNSVPQTG 

       670        680        690        700        710        720 
TTTSSLAANS LRLSQPYDTL LRSPTKAFHI TAMEAYTPER GSANRARSPL HSNSNNTNNN 

       730        740        750        760        770        780 
GANNSNLQTS GMENKQTGLV LDSNVLKSME SNNDNRRLTP STSKSQNVKS SPGVWNLLQF 

       790        800        810        820        830        840 
SSTNNTPAAD SGGNKRGFSI NPDIKAKENE NATSEKDSDS NSNDLETKDI NSSPLKNQGG 

       850        860 
STANAKELIL DTDGAKISII NN 

« Hide

References

« Hide 'large scale' references
[1]"Two fork head homologs in S. cerevisiae."
Zhu G., Davis T.N.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
Poehlmann R., Philippsen P.
Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
Bergez P., Doignon F., Crouzet M.
Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-440.
Strain: S288c / FY1676.
[6]Erratum
Bergez P., Doignon F., Crouzet M.
Yeast 12:297-297(1996) [PubMed] [Europe PMC] [Abstract]
[7]"Recruitment of Thr 319-phosphorylated Ndd1p to the FHA domain of Fkh2p requires Clb kinase activity: a mechanism for CLB cluster gene activation."
Reynolds D., Shi B.J., McLean C., Katsis F., Kemp B., Dalton S.
Genes Dev. 17:1789-1802(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN, INTERACTION WITH NDD1.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-832 AND SER-833, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-708; SER-832 AND SER-833, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L38850 Genomic DNA. Translation: AAA60939.1.
X86470 Genomic DNA. Translation: CAA60193.1.
Z71343 Genomic DNA. Translation: CAA95941.1.
Z71344 Genomic DNA. Translation: CAA95942.1.
U12141 Genomic DNA. Translation: AAA99643.1.
BK006947 Genomic DNA. Translation: DAA10477.1.
PIRS53913.
RefSeqNP_014331.3. NM_001182906.3.

3D structure databases

ProteinModelPortalP41813.
SMRP41813. Positions 81-172, 340-427.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35755. 131 interactions.
DIPDIP-5944N.
IntActP41813. 1 interaction.
MINTMINT-673870.
STRING4932.YNL068C.

Proteomic databases

PaxDbP41813.
PeptideAtlasP41813.
PRIDEP41813.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL068C; YNL068C; YNL068C.
GeneID855656.
KEGGsce:YNL068C.

Organism-specific databases

CYGDYNL068c.
SGDS000005012. FKH2.

Phylogenomic databases

eggNOGCOG5025.
GeneTreeENSGT00750000117242.
HOGENOMHOG000093809.
OMAGMKWRIS.
OrthoDBEOG7647BS.

Enzyme and pathway databases

BioCycYEAST:G3O-33098-MONOMER.

Gene expression databases

GenevestigatorP41813.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.200.20. 1 hit.
InterProIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00498. FHA. 1 hit.
PF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSPR00053. FORKHEAD.
SMARTSM00339. FH. 1 hit.
SM00240. FHA. 1 hit.
[Graphical view]
SUPFAMSSF49879. SSF49879. 1 hit.
PROSITEPS50006. FHA_DOMAIN. 1 hit.
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979914.

Entry information

Entry nameFKH2_YEAST
AccessionPrimary (citable) accession number: P41813
Secondary accession number(s): D6W1B1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families