Coatomer subunit beta' - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P41811 (COPB2_YEAST)

Last modified February 9, 2010. Version 95. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Coatomer subunit beta'
Alternative name(s):
    Beta'-coat protein
      Short name=Beta'-COP

Gene names

Name:	SEC27
Ordered Locus Names:	YGL137W
ORF Names:	G2827

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	889 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Ref.7
Subunit structure	Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with the ESCRT-0 subunit VPS27. Ref.7
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesicle › COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it By similarity. Ref.7 Ref.5
Miscellaneous	Present with 129000 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 6 WD repeats.

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Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Ref.1 Inferred from mutant phenotype. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro late endosome to vacuole transport via multivesicular body sorting pathway Ref.7 Inferred from mutant phenotype. Source: SGD retrograde vesicle-mediated transport, Golgi to ER Inferred from mutant phenotype. Source: SGD
Cellular component	COPI vesicle coat Ref.1 Inferred from genetic interaction. Source: SGD extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.7 Inferred from physical interaction. Source: IntAct structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
	P53839	1	EBI-4898,EBI-28263
	Q03687	1	EBI-4898,EBI-28024
ACB1	P31787	1	EBI-4898,EBI-2060
ADE17	P38009	1	EBI-4898,EBI-14223
ARC18	Q05933	1	EBI-4898,EBI-2764
AUR1	P36107	1	EBI-4898,EBI-2046036
DNM1	P54861	1	EBI-4898,EBI-6002
INO1	P11986	1	EBI-4898,EBI-9257
KEX1	P09620	1	EBI-4898,EBI-9653
LSP1	Q12230	1	EBI-4898,EBI-34978
PGM2	P37012	1	EBI-4898,EBI-13296
PLM2	Q04383	1	EBI-4898,EBI-2079237
PRO2	P54885	1	EBI-4898,EBI-13872
PRO3	P32263	1	EBI-4898,EBI-13885
RAD25	Q00578	1	EBI-4898,EBI-14683
RBD2	Q12270	1	EBI-4898,EBI-31471
RET1	P53622	2	EBI-4898,EBI-4860
RET3	P53600	1	EBI-4898,EBI-4905
SEC18	P18759	1	EBI-4898,EBI-16565
SEC28	P40509	2	EBI-4898,EBI-4884
SIS1	P25294	1	EBI-4898,EBI-17244
SLY1	P22213	1	EBI-4898,EBI-17387
SMI1	P32566	1	EBI-4898,EBI-17452
SNA3	P14359	1	EBI-4898,EBI-26122
SNC2	P33328	1	EBI-4898,EBI-17512
SRV2	P17555	1	EBI-4898,EBI-4024
TRP2	P00899	1	EBI-4898,EBI-19575
TVP15	Q03860	1	EBI-4898,EBI-37537
URA7	P28274	1	EBI-4898,EBI-20128
VPS27	P40343	1	EBI-4898,EBI-20380
YOP1	Q12402	1	EBI-4898,EBI-37092

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 889

889

Coatomer subunit beta'

PRO_0000050918

Regions

Repeat

11 – 41

WD 1

Repeat

53 – 83

WD 2

Repeat

95 – 125

WD 3

Repeat

138 – 169

WD 4

Repeat

182 – 214

WD 5

Repeat

226 – 256

WD 6

Amino acid modifications

Modified residue

669

Phosphothreonine Ref.8

Modified residue

829

Phosphothreonine Ref.8

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Sequences

Sequence

Length

Mass (Da)

Tools

P41811-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 6A5E50BBEB02CB58
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FASTA

889

99,445

        10         20         30         40         50         60 
MKLDIKKTFS NRSDRVKGID FHPTEPWVLT TLYSGRVELW NYETQVEVRS IQVTETPVRA 

        70         80         90        100        110        120 
GKFIARKNWI IVGSDDFRIR VFNYNTGEKV VDFEAHPDYI RSIAVHPTKP YVLSGSDDLT 

       130        140        150        160        170        180 
VKLWNWENNW ALEQTFEGHE HFVMCVAFNP KDPSTFASGC LDRTVKVWSL GQSTPNFTLT 

       190        200        210        220        230        240 
TGQERGVNYV DYYPLPDKPY MITASDDLTI KIWDYQTKSC VATLEGHMSN VSFAVFHPTL 

       250        260        270        280        290        300 
PIIISGSEDG TLKIWNSSTY KVEKTLNVGL ERSWCIATHP TGRKNYIASG FDNGFTVLSL 

       310        320        330        340        350        360 
GNDEPTLSLD PVGKLVWSGG KNAAASDIFT AVIRGNEEVE QDEPLSLQTK ELGSVDVFPQ 

       370        380        390        400        410        420 
SLAHSPNGRF VTVVGDGEYV IYTALAWRNK AFGKCQDFVW GPDSNSYALI DETGQIKYYK 

       430        440        450        460        470        480 
NFKEVTSWSV PMHSAIDRLF SGALLGVKSD GFVYFFDWDN GTLVRRIDVN AKDVIWSDNG 

       490        500        510        520        530        540 
ELVMIVNTNS NGDEASGYTL LFNKDAYLEA ANNGNIDDSE GVDEAFDVLY ELSESITSGK 

       550        560        570        580        590        600 
WVGDVFIFTT ATNRLNYFVG GKTYNLAHYT KEMYLLGYLA RDNKVYLADR EVHVYGYEIS 

       610        620        630        640        650        660 
LEVLEFQTLT LRGEIEEAIE NVLPNVEGKD SLTKIARFLE GQEYYEEALN ISPDQDQKFE 

       670        680        690        700        710        720 
LALKVGQLTL ARDLLTDESA EMKWRALGDA SLQRFNFKLA VEAFTNAHDL ESLFLLHSSF 

       730        740        750        760        770        780 
NNKEGLVTLA KDAERAGKFN LAFNAYWIAG DIQGAKDLLI KSQRFSEAAF LGSTYGLGDD 

       790        800        810        820        830        840 
AVNDIVTKWK ENLILNGKNT VSERVCGAEG LPGSSSSGDA QPLIDLDSTP APEQADENKE 

       850        860        870        880 
AEVEDSEFKE SNSEAVEAEK KEEEAPQQQQ SEQQPEQGEA VPEPVEEES

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic." Duden R., Hosobuchi M., Hamamoto S., Winey M., Byers B., Schekman R. J. Biol. Chem. 269:24486-24495(1994) [PubMed: 7929113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-11. Strain: RSY255.
[2]	"Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames." Escribano V., Eraso P., Portillo F., Mazon M.J. Yeast 12:887-892(1996) [PubMed: 8840506] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Yeast coatomer contains a subunit homologous to mammalian beta'-COP." Harter C., Draken E., Lottspeich F., Wieland F.T. FEBS Lett. 332:71-73(1993) [PubMed: 8405452] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-27. Strain: ATCC 208279 / BJ926.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast." Gabriely G., Kama R., Gerst J.E. Mol. Cell. Biol. 27:526-540(2007) [PubMed: 17101773] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS27.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-669 AND THR-829, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	U11237 Genomic DNA. Translation: AAA61711.1. X92670 Genomic DNA. Translation: CAA63359.1. Z72659 Genomic DNA. Translation: CAA96848.1.
PIR	B55123.
RefSeq	NP_011378.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6468N.
IntAct	P41811. 118 interactions.
STRING	P41811.
Proteomic databases
PeptideAtlas	P41811.
PRIDE	P41811.
Genome annotation databases
Ensembl	YGL137W; YGL137W; YGL137W; Saccharomyces cerevisiae. [Genome view]
GeneID	852740.
KEGG	sce:YGL137W.
NMPDR	fig\|4932.3.peg.2482.
Organism-specific databases
CYGD	YGL137w.
SGD	S000003105. SEC27.
Phylogenomic databases
eggNOG	fuNOG05815.
HOGENOM	HBG330931.
OMA	FYDWENT.
OrthoDB	EOG99S7PG.
PhylomeDB	P41811.
Gene expression databases
ArrayExpress	P41811.
Genevestigator	P41811.
GermOnline	YGL137W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016453. Coatomer_beta'_subunit. IPR006692. Coatomer_WD-assoc_reg. IPR020472. G-protein_beta_WD-40_rep_reg. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF04053. Coatomer_WDAD. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
PIRSF	PIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972153.

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Entry information

Entry name

COPB2_YEAST

Accession

Primary (citable) accession number: P41811

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents