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Protein

Coatomer subunit beta'

Gene

SEC27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.1 Publication

GO - Molecular functioni

  • structural molecule activity Source: InterPro
  • ubiquitin binding Source: SGD

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • intracellular protein transport Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: GO_Central
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30632-MONOMER.
ReactomeiR-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit beta'
Alternative name(s):
Beta'-coat protein
Short name:
Beta'-COP
Gene namesi
Name:SEC27
Ordered Locus Names:YGL137W
ORF Names:G2827
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL137W.
SGDiS000003105. SEC27.

Subcellular locationi

GO - Cellular componenti

  • COPI vesicle coat Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509181 – 889Coatomer subunit beta'Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei326PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41811.
PRIDEiP41811.

PTM databases

iPTMnetiP41811.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with the ESCRT-0 subunit VPS27.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
COP1P5362217EBI-4898,EBI-4860
RET3P536003EBI-4898,EBI-4905
SEC28P405094EBI-4898,EBI-4884

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi33115. 251 interactors.
DIPiDIP-6468N.
IntActiP41811. 71 interactors.
MINTiMINT-428887.

Structurei

Secondary structure

1889
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Beta strandi16 – 21Combined sources6
Beta strandi23 – 32Combined sources10
Beta strandi35 – 41Combined sources7
Turni42 – 45Combined sources4
Beta strandi46 – 52Combined sources7
Beta strandi58 – 64Combined sources7
Helixi65 – 67Combined sources3
Beta strandi69 – 74Combined sources6
Beta strandi77 – 83Combined sources7
Turni84 – 86Combined sources3
Beta strandi89 – 94Combined sources6
Beta strandi100 – 105Combined sources6
Beta strandi107 – 116Combined sources10
Beta strandi121 – 125Combined sources5
Turni126 – 130Combined sources5
Beta strandi132 – 136Combined sources5
Beta strandi143 – 148Combined sources6
Beta strandi155 – 160Combined sources6
Beta strandi163 – 169Combined sources7
Beta strandi176 – 180Combined sources5
Beta strandi189 – 192Combined sources4
Beta strandi200 – 204Combined sources5
Beta strandi208 – 214Combined sources7
Turni215 – 217Combined sources3
Beta strandi220 – 225Combined sources6
Beta strandi231 – 236Combined sources6
Beta strandi238 – 247Combined sources10
Beta strandi252 – 256Combined sources5
Turni257 – 259Combined sources3
Beta strandi262 – 266Combined sources5
Beta strandi269 – 278Combined sources10
Helixi283 – 285Combined sources3
Beta strandi287 – 291Combined sources5
Beta strandi294 – 299Combined sources6
Beta strandi313 – 318Combined sources6
Beta strandi321 – 323Combined sources3
Beta strandi327 – 332Combined sources6
Beta strandi341 – 343Combined sources3
Beta strandi350 – 354Combined sources5
Beta strandi360 – 364Combined sources5
Beta strandi368 – 375Combined sources8
Beta strandi378 – 383Combined sources6
Turni384 – 386Combined sources3
Beta strandi389 – 394Combined sources6
Beta strandi396 – 400Combined sources5
Beta strandi404 – 410Combined sources7
Beta strandi416 – 420Combined sources5
Beta strandi423 – 427Combined sources5
Beta strandi438 – 440Combined sources3
Beta strandi442 – 448Combined sources7
Beta strandi453 – 456Combined sources4
Turni458 – 460Combined sources3
Beta strandi463 – 467Combined sources5
Beta strandi471 – 476Combined sources6
Beta strandi480 – 487Combined sources8
Turni490 – 493Combined sources4
Beta strandi496 – 502Combined sources7
Helixi504 – 512Combined sources9
Beta strandi518 – 520Combined sources3
Helixi523 – 525Combined sources3
Beta strandi526 – 532Combined sources7
Beta strandi537 – 542Combined sources6
Beta strandi545 – 549Combined sources5
Beta strandi554 – 558Combined sources5
Beta strandi563 – 568Combined sources6
Beta strandi573 – 579Combined sources7
Turni580 – 583Combined sources4
Beta strandi584 – 589Combined sources6
Beta strandi594 – 598Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YNNX-ray1.78A1-304[»]
2YNOX-ray1.80A/B1-304[»]
2YNPX-ray2.96A1-604[»]
4J73X-ray1.44A1-301[»]
4J77X-ray1.76A/B1-301[»]
4J78X-ray1.48A1-301[»]
4J79X-ray1.56A1-300[»]
4J81X-ray1.74A/B1-301[»]
4J82X-ray1.46A/B1-301[»]
4J84X-ray1.47A/B1-301[»]
4J86X-ray1.48A/B1-301[»]
ProteinModelPortaliP41811.
SMRiP41811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 41WD 1Add BLAST31
Repeati53 – 83WD 2Add BLAST31
Repeati95 – 125WD 3Add BLAST31
Repeati138 – 169WD 4Add BLAST32
Repeati182 – 214WD 5Add BLAST33
Repeati226 – 256WD 6Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat COPB2 family.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00780000122009.
HOGENOMiHOG000163444.
KOiK17302.
OMAiWASKEYS.
OrthoDBiEOG092C10HM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 4 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDIKKTFS NRSDRVKGID FHPTEPWVLT TLYSGRVELW NYETQVEVRS
60 70 80 90 100
IQVTETPVRA GKFIARKNWI IVGSDDFRIR VFNYNTGEKV VDFEAHPDYI
110 120 130 140 150
RSIAVHPTKP YVLSGSDDLT VKLWNWENNW ALEQTFEGHE HFVMCVAFNP
160 170 180 190 200
KDPSTFASGC LDRTVKVWSL GQSTPNFTLT TGQERGVNYV DYYPLPDKPY
210 220 230 240 250
MITASDDLTI KIWDYQTKSC VATLEGHMSN VSFAVFHPTL PIIISGSEDG
260 270 280 290 300
TLKIWNSSTY KVEKTLNVGL ERSWCIATHP TGRKNYIASG FDNGFTVLSL
310 320 330 340 350
GNDEPTLSLD PVGKLVWSGG KNAAASDIFT AVIRGNEEVE QDEPLSLQTK
360 370 380 390 400
ELGSVDVFPQ SLAHSPNGRF VTVVGDGEYV IYTALAWRNK AFGKCQDFVW
410 420 430 440 450
GPDSNSYALI DETGQIKYYK NFKEVTSWSV PMHSAIDRLF SGALLGVKSD
460 470 480 490 500
GFVYFFDWDN GTLVRRIDVN AKDVIWSDNG ELVMIVNTNS NGDEASGYTL
510 520 530 540 550
LFNKDAYLEA ANNGNIDDSE GVDEAFDVLY ELSESITSGK WVGDVFIFTT
560 570 580 590 600
ATNRLNYFVG GKTYNLAHYT KEMYLLGYLA RDNKVYLADR EVHVYGYEIS
610 620 630 640 650
LEVLEFQTLT LRGEIEEAIE NVLPNVEGKD SLTKIARFLE GQEYYEEALN
660 670 680 690 700
ISPDQDQKFE LALKVGQLTL ARDLLTDESA EMKWRALGDA SLQRFNFKLA
710 720 730 740 750
VEAFTNAHDL ESLFLLHSSF NNKEGLVTLA KDAERAGKFN LAFNAYWIAG
760 770 780 790 800
DIQGAKDLLI KSQRFSEAAF LGSTYGLGDD AVNDIVTKWK ENLILNGKNT
810 820 830 840 850
VSERVCGAEG LPGSSSSGDA QPLIDLDSTP APEQADENKE AEVEDSEFKE
860 870 880
SNSEAVEAEK KEEEAPQQQQ SEQQPEQGEA VPEPVEEES
Length:889
Mass (Da):99,445
Last modified:November 1, 1995 - v1
Checksum:i6A5E50BBEB02CB58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11237 Genomic DNA. Translation: AAA61711.1.
X92670 Genomic DNA. Translation: CAA63359.1.
Z72659 Genomic DNA. Translation: CAA96848.1.
BK006941 Genomic DNA. Translation: DAA07973.1.
PIRiB55123.
RefSeqiNP_011378.1. NM_001181002.1.

Genome annotation databases

EnsemblFungiiYGL137W; YGL137W; YGL137W.
GeneIDi852740.
KEGGisce:YGL137W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11237 Genomic DNA. Translation: AAA61711.1.
X92670 Genomic DNA. Translation: CAA63359.1.
Z72659 Genomic DNA. Translation: CAA96848.1.
BK006941 Genomic DNA. Translation: DAA07973.1.
PIRiB55123.
RefSeqiNP_011378.1. NM_001181002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YNNX-ray1.78A1-304[»]
2YNOX-ray1.80A/B1-304[»]
2YNPX-ray2.96A1-604[»]
4J73X-ray1.44A1-301[»]
4J77X-ray1.76A/B1-301[»]
4J78X-ray1.48A1-301[»]
4J79X-ray1.56A1-300[»]
4J81X-ray1.74A/B1-301[»]
4J82X-ray1.46A/B1-301[»]
4J84X-ray1.47A/B1-301[»]
4J86X-ray1.48A/B1-301[»]
ProteinModelPortaliP41811.
SMRiP41811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33115. 251 interactors.
DIPiDIP-6468N.
IntActiP41811. 71 interactors.
MINTiMINT-428887.

PTM databases

iPTMnetiP41811.

Proteomic databases

MaxQBiP41811.
PRIDEiP41811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL137W; YGL137W; YGL137W.
GeneIDi852740.
KEGGisce:YGL137W.

Organism-specific databases

EuPathDBiFungiDB:YGL137W.
SGDiS000003105. SEC27.

Phylogenomic databases

GeneTreeiENSGT00780000122009.
HOGENOMiHOG000163444.
KOiK17302.
OMAiWASKEYS.
OrthoDBiEOG092C10HM.

Enzyme and pathway databases

BioCyciYEAST:G3O-30632-MONOMER.
ReactomeiR-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiP41811.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 4 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOPB2_YEAST
AccessioniPrimary (citable) accession number: P41811
Secondary accession number(s): D6VU12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 129000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.