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P41811

- COPB2_YEAST

UniProt

P41811 - COPB2_YEAST

Protein

Coatomer subunit beta'

Gene

SEC27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. structural molecule activity Source: InterPro
    3. ubiquitin binding Source: SGD

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: SGD
    2. intracellular protein transport Source: InterPro
    3. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
    4. retrograde vesicle-mediated transport, Golgi to ER Source: SGD

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30632-MONOMER.
    ReactomeiREACT_33101. COPI Mediated Transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coatomer subunit beta'
    Alternative name(s):
    Beta'-coat protein
    Short name:
    Beta'-COP
    Gene namesi
    Name:SEC27
    Ordered Locus Names:YGL137W
    ORF Names:G2827
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL137w.
    SGDiS000003105. SEC27.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesicleCOPI-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.By similarity

    GO - Cellular componenti

    1. COPI vesicle coat Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 889889Coatomer subunit beta'PRO_0000050918Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei326 – 3261Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP41811.
    PaxDbiP41811.
    PeptideAtlasiP41811.

    Expressioni

    Gene expression databases

    GenevestigatoriP41811.

    Interactioni

    Subunit structurei

    Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with the ESCRT-0 subunit VPS27.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    COP1P5362217EBI-4898,EBI-4860
    RET3P536003EBI-4898,EBI-4905
    SEC28P405094EBI-4898,EBI-4884

    Protein-protein interaction databases

    BioGridi33115. 246 interactions.
    DIPiDIP-6468N.
    IntActiP41811. 70 interactions.
    MINTiMINT-428887.
    STRINGi4932.YGL137W.

    Structurei

    Secondary structure

    1
    889
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 127
    Beta strandi16 – 216
    Beta strandi23 – 3210
    Beta strandi35 – 417
    Turni42 – 454
    Beta strandi46 – 527
    Beta strandi58 – 647
    Helixi65 – 673
    Beta strandi69 – 746
    Beta strandi77 – 837
    Turni84 – 863
    Beta strandi89 – 946
    Beta strandi100 – 1056
    Beta strandi107 – 11610
    Beta strandi121 – 1255
    Turni126 – 1305
    Beta strandi132 – 1365
    Beta strandi143 – 1486
    Beta strandi155 – 1606
    Beta strandi163 – 1697
    Beta strandi176 – 1805
    Beta strandi189 – 1924
    Beta strandi200 – 2045
    Beta strandi208 – 2147
    Turni215 – 2173
    Beta strandi220 – 2256
    Beta strandi231 – 2366
    Beta strandi238 – 24710
    Beta strandi252 – 2565
    Turni257 – 2593
    Beta strandi262 – 2665
    Beta strandi269 – 27810
    Helixi283 – 2853
    Beta strandi287 – 2915
    Beta strandi294 – 2996
    Beta strandi313 – 3186
    Beta strandi321 – 3233
    Beta strandi327 – 3326
    Beta strandi341 – 3433
    Beta strandi350 – 3545
    Beta strandi360 – 3645
    Beta strandi368 – 3758
    Beta strandi378 – 3836
    Turni384 – 3863
    Beta strandi389 – 3946
    Beta strandi396 – 4005
    Beta strandi404 – 4107
    Beta strandi416 – 4205
    Beta strandi423 – 4275
    Beta strandi438 – 4403
    Beta strandi442 – 4487
    Beta strandi453 – 4564
    Turni458 – 4603
    Beta strandi463 – 4675
    Beta strandi471 – 4766
    Beta strandi480 – 4878
    Turni490 – 4934
    Beta strandi496 – 5027
    Helixi504 – 5129
    Beta strandi518 – 5203
    Helixi523 – 5253
    Beta strandi526 – 5327
    Beta strandi537 – 5426
    Beta strandi545 – 5495
    Beta strandi554 – 5585
    Beta strandi563 – 5686
    Beta strandi573 – 5797
    Turni580 – 5834
    Beta strandi584 – 5896
    Beta strandi594 – 5985

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YNNX-ray1.78A1-304[»]
    2YNOX-ray1.80A/B1-304[»]
    2YNPX-ray2.96A1-604[»]
    4J73X-ray1.44A1-301[»]
    4J77X-ray1.76A/B1-301[»]
    4J78X-ray1.48A1-301[»]
    4J79X-ray1.56A1-300[»]
    4J81X-ray1.74A/B1-301[»]
    4J82X-ray1.46A/B1-301[»]
    4J84X-ray1.47A/B1-301[»]
    4J86X-ray1.48A/B1-301[»]
    ProteinModelPortaliP41811.
    SMRiP41811. Positions 1-812.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati11 – 4131WD 1Add
    BLAST
    Repeati53 – 8331WD 2Add
    BLAST
    Repeati95 – 12531WD 3Add
    BLAST
    Repeati138 – 16932WD 4Add
    BLAST
    Repeati182 – 21433WD 5Add
    BLAST
    Repeati226 – 25631WD 6Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat COPB2 family.Curated
    Contains 6 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00740000115578.
    HOGENOMiHOG000163444.
    KOiK17302.
    OMAiIAHNPNG.
    OrthoDBiEOG7TN016.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    InterProiIPR006692. Coatomer_WD-assoc_reg.
    IPR016453. COPB2.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF04053. Coatomer_WDAD. 1 hit.
    PF00400. WD40. 4 hits.
    [Graphical view]
    PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 4 hits.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41811-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLDIKKTFS NRSDRVKGID FHPTEPWVLT TLYSGRVELW NYETQVEVRS    50
    IQVTETPVRA GKFIARKNWI IVGSDDFRIR VFNYNTGEKV VDFEAHPDYI 100
    RSIAVHPTKP YVLSGSDDLT VKLWNWENNW ALEQTFEGHE HFVMCVAFNP 150
    KDPSTFASGC LDRTVKVWSL GQSTPNFTLT TGQERGVNYV DYYPLPDKPY 200
    MITASDDLTI KIWDYQTKSC VATLEGHMSN VSFAVFHPTL PIIISGSEDG 250
    TLKIWNSSTY KVEKTLNVGL ERSWCIATHP TGRKNYIASG FDNGFTVLSL 300
    GNDEPTLSLD PVGKLVWSGG KNAAASDIFT AVIRGNEEVE QDEPLSLQTK 350
    ELGSVDVFPQ SLAHSPNGRF VTVVGDGEYV IYTALAWRNK AFGKCQDFVW 400
    GPDSNSYALI DETGQIKYYK NFKEVTSWSV PMHSAIDRLF SGALLGVKSD 450
    GFVYFFDWDN GTLVRRIDVN AKDVIWSDNG ELVMIVNTNS NGDEASGYTL 500
    LFNKDAYLEA ANNGNIDDSE GVDEAFDVLY ELSESITSGK WVGDVFIFTT 550
    ATNRLNYFVG GKTYNLAHYT KEMYLLGYLA RDNKVYLADR EVHVYGYEIS 600
    LEVLEFQTLT LRGEIEEAIE NVLPNVEGKD SLTKIARFLE GQEYYEEALN 650
    ISPDQDQKFE LALKVGQLTL ARDLLTDESA EMKWRALGDA SLQRFNFKLA 700
    VEAFTNAHDL ESLFLLHSSF NNKEGLVTLA KDAERAGKFN LAFNAYWIAG 750
    DIQGAKDLLI KSQRFSEAAF LGSTYGLGDD AVNDIVTKWK ENLILNGKNT 800
    VSERVCGAEG LPGSSSSGDA QPLIDLDSTP APEQADENKE AEVEDSEFKE 850
    SNSEAVEAEK KEEEAPQQQQ SEQQPEQGEA VPEPVEEES 889
    Length:889
    Mass (Da):99,445
    Last modified:November 1, 1995 - v1
    Checksum:i6A5E50BBEB02CB58
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U11237 Genomic DNA. Translation: AAA61711.1.
    X92670 Genomic DNA. Translation: CAA63359.1.
    Z72659 Genomic DNA. Translation: CAA96848.1.
    BK006941 Genomic DNA. Translation: DAA07973.1.
    PIRiB55123.
    RefSeqiNP_011378.1. NM_001181002.1.

    Genome annotation databases

    EnsemblFungiiYGL137W; YGL137W; YGL137W.
    GeneIDi852740.
    KEGGisce:YGL137W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U11237 Genomic DNA. Translation: AAA61711.1 .
    X92670 Genomic DNA. Translation: CAA63359.1 .
    Z72659 Genomic DNA. Translation: CAA96848.1 .
    BK006941 Genomic DNA. Translation: DAA07973.1 .
    PIRi B55123.
    RefSeqi NP_011378.1. NM_001181002.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YNN X-ray 1.78 A 1-304 [» ]
    2YNO X-ray 1.80 A/B 1-304 [» ]
    2YNP X-ray 2.96 A 1-604 [» ]
    4J73 X-ray 1.44 A 1-301 [» ]
    4J77 X-ray 1.76 A/B 1-301 [» ]
    4J78 X-ray 1.48 A 1-301 [» ]
    4J79 X-ray 1.56 A 1-300 [» ]
    4J81 X-ray 1.74 A/B 1-301 [» ]
    4J82 X-ray 1.46 A/B 1-301 [» ]
    4J84 X-ray 1.47 A/B 1-301 [» ]
    4J86 X-ray 1.48 A/B 1-301 [» ]
    ProteinModelPortali P41811.
    SMRi P41811. Positions 1-812.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33115. 246 interactions.
    DIPi DIP-6468N.
    IntActi P41811. 70 interactions.
    MINTi MINT-428887.
    STRINGi 4932.YGL137W.

    Proteomic databases

    MaxQBi P41811.
    PaxDbi P41811.
    PeptideAtlasi P41811.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL137W ; YGL137W ; YGL137W .
    GeneIDi 852740.
    KEGGi sce:YGL137W.

    Organism-specific databases

    CYGDi YGL137w.
    SGDi S000003105. SEC27.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00740000115578.
    HOGENOMi HOG000163444.
    KOi K17302.
    OMAi IAHNPNG.
    OrthoDBi EOG7TN016.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30632-MONOMER.
    Reactomei REACT_33101. COPI Mediated Transport.

    Miscellaneous databases

    NextBioi 972153.
    PROi P41811.

    Gene expression databases

    Genevestigatori P41811.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    InterProi IPR006692. Coatomer_WD-assoc_reg.
    IPR016453. COPB2.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF04053. Coatomer_WDAD. 1 hit.
    PF00400. WD40. 4 hits.
    [Graphical view ]
    PIRSFi PIRSF005567. Coatomer_beta'_subunit. 1 hit.
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 4 hits.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic."
      Duden R., Hosobuchi M., Hamamoto S., Winey M., Byers B., Schekman R.
      J. Biol. Chem. 269:24486-24495(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-11.
      Strain: RSY255.
    2. "Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames."
      Escribano V., Eraso P., Portillo F., Mazon M.J.
      Yeast 12:887-892(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Yeast coatomer contains a subunit homologous to mammalian beta'-COP."
      Harter C., Draken E., Lottspeich F., Wieland F.T.
      FEBS Lett. 332:71-73(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-27.
      Strain: ATCC 208279 / BJ926.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast."
      Gabriely G., Kama R., Gerst J.E.
      Mol. Cell. Biol. 27:526-540(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS27.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCOPB2_YEAST
    AccessioniPrimary (citable) accession number: P41811
    Secondary accession number(s): D6VU12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 129000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3