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P41811

- COPB2_YEAST

UniProt

P41811 - COPB2_YEAST

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Protein

Coatomer subunit beta'

Gene
SEC27, YGL137W, G2827
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. structural molecule activity Source: InterPro
  3. ubiquitin binding Source: SGD

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
  2. intracellular protein transport Source: InterPro
  3. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  4. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30632-MONOMER.
ReactomeiREACT_33101. COPI Mediated Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit beta'
Alternative name(s):
Beta'-coat protein
Short name:
Beta'-COP
Gene namesi
Name:SEC27
Ordered Locus Names:YGL137W
ORF Names:G2827
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL137w.
SGDiS000003105. SEC27.

Subcellular locationi

Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesicleCOPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity
Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it By similarity.2 Publications

GO - Cellular componenti

  1. COPI vesicle coat Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 889889Coatomer subunit beta'PRO_0000050918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei326 – 3261Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41811.
PaxDbiP41811.
PeptideAtlasiP41811.

Expressioni

Gene expression databases

GenevestigatoriP41811.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with the ESCRT-0 subunit VPS27.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
COP1P5362217EBI-4898,EBI-4860
RET3P536003EBI-4898,EBI-4905
SEC28P405094EBI-4898,EBI-4884

Protein-protein interaction databases

BioGridi33115. 246 interactions.
DIPiDIP-6468N.
IntActiP41811. 70 interactions.
MINTiMINT-428887.
STRINGi4932.YGL137W.

Structurei

Secondary structure

1
889
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127
Beta strandi16 – 216
Beta strandi23 – 3210
Beta strandi35 – 417
Turni42 – 454
Beta strandi46 – 527
Beta strandi58 – 647
Helixi65 – 673
Beta strandi69 – 746
Beta strandi77 – 837
Turni84 – 863
Beta strandi89 – 946
Beta strandi100 – 1056
Beta strandi107 – 11610
Beta strandi121 – 1255
Turni126 – 1305
Beta strandi132 – 1365
Beta strandi143 – 1486
Beta strandi155 – 1606
Beta strandi163 – 1697
Beta strandi176 – 1805
Beta strandi189 – 1924
Beta strandi200 – 2045
Beta strandi208 – 2147
Turni215 – 2173
Beta strandi220 – 2256
Beta strandi231 – 2366
Beta strandi238 – 24710
Beta strandi252 – 2565
Turni257 – 2593
Beta strandi262 – 2665
Beta strandi269 – 27810
Helixi283 – 2853
Beta strandi287 – 2915
Beta strandi294 – 2996
Beta strandi313 – 3186
Beta strandi321 – 3233
Beta strandi327 – 3326
Beta strandi341 – 3433
Beta strandi350 – 3545
Beta strandi360 – 3645
Beta strandi368 – 3758
Beta strandi378 – 3836
Turni384 – 3863
Beta strandi389 – 3946
Beta strandi396 – 4005
Beta strandi404 – 4107
Beta strandi416 – 4205
Beta strandi423 – 4275
Beta strandi438 – 4403
Beta strandi442 – 4487
Beta strandi453 – 4564
Turni458 – 4603
Beta strandi463 – 4675
Beta strandi471 – 4766
Beta strandi480 – 4878
Turni490 – 4934
Beta strandi496 – 5027
Helixi504 – 5129
Beta strandi518 – 5203
Helixi523 – 5253
Beta strandi526 – 5327
Beta strandi537 – 5426
Beta strandi545 – 5495
Beta strandi554 – 5585
Beta strandi563 – 5686
Beta strandi573 – 5797
Turni580 – 5834
Beta strandi584 – 5896
Beta strandi594 – 5985

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YNNX-ray1.78A1-304[»]
2YNOX-ray1.80A/B1-304[»]
2YNPX-ray2.96A1-604[»]
4J73X-ray1.44A1-301[»]
4J77X-ray1.76A/B1-301[»]
4J78X-ray1.48A1-301[»]
4J79X-ray1.56A1-300[»]
4J81X-ray1.74A/B1-301[»]
4J82X-ray1.46A/B1-301[»]
4J84X-ray1.47A/B1-301[»]
4J86X-ray1.48A/B1-301[»]
ProteinModelPortaliP41811.
SMRiP41811. Positions 1-812.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 4131WD 1Add
BLAST
Repeati53 – 8331WD 2Add
BLAST
Repeati95 – 12531WD 3Add
BLAST
Repeati138 – 16932WD 4Add
BLAST
Repeati182 – 21433WD 5Add
BLAST
Repeati226 – 25631WD 6Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat COPB2 family.
Contains 6 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00740000115578.
HOGENOMiHOG000163444.
KOiK17302.
OMAiIAHNPNG.
OrthoDBiEOG7TN016.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 4 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41811-1 [UniParc]FASTAAdd to Basket

« Hide

MKLDIKKTFS NRSDRVKGID FHPTEPWVLT TLYSGRVELW NYETQVEVRS    50
IQVTETPVRA GKFIARKNWI IVGSDDFRIR VFNYNTGEKV VDFEAHPDYI 100
RSIAVHPTKP YVLSGSDDLT VKLWNWENNW ALEQTFEGHE HFVMCVAFNP 150
KDPSTFASGC LDRTVKVWSL GQSTPNFTLT TGQERGVNYV DYYPLPDKPY 200
MITASDDLTI KIWDYQTKSC VATLEGHMSN VSFAVFHPTL PIIISGSEDG 250
TLKIWNSSTY KVEKTLNVGL ERSWCIATHP TGRKNYIASG FDNGFTVLSL 300
GNDEPTLSLD PVGKLVWSGG KNAAASDIFT AVIRGNEEVE QDEPLSLQTK 350
ELGSVDVFPQ SLAHSPNGRF VTVVGDGEYV IYTALAWRNK AFGKCQDFVW 400
GPDSNSYALI DETGQIKYYK NFKEVTSWSV PMHSAIDRLF SGALLGVKSD 450
GFVYFFDWDN GTLVRRIDVN AKDVIWSDNG ELVMIVNTNS NGDEASGYTL 500
LFNKDAYLEA ANNGNIDDSE GVDEAFDVLY ELSESITSGK WVGDVFIFTT 550
ATNRLNYFVG GKTYNLAHYT KEMYLLGYLA RDNKVYLADR EVHVYGYEIS 600
LEVLEFQTLT LRGEIEEAIE NVLPNVEGKD SLTKIARFLE GQEYYEEALN 650
ISPDQDQKFE LALKVGQLTL ARDLLTDESA EMKWRALGDA SLQRFNFKLA 700
VEAFTNAHDL ESLFLLHSSF NNKEGLVTLA KDAERAGKFN LAFNAYWIAG 750
DIQGAKDLLI KSQRFSEAAF LGSTYGLGDD AVNDIVTKWK ENLILNGKNT 800
VSERVCGAEG LPGSSSSGDA QPLIDLDSTP APEQADENKE AEVEDSEFKE 850
SNSEAVEAEK KEEEAPQQQQ SEQQPEQGEA VPEPVEEES 889
Length:889
Mass (Da):99,445
Last modified:November 1, 1995 - v1
Checksum:i6A5E50BBEB02CB58
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U11237 Genomic DNA. Translation: AAA61711.1.
X92670 Genomic DNA. Translation: CAA63359.1.
Z72659 Genomic DNA. Translation: CAA96848.1.
BK006941 Genomic DNA. Translation: DAA07973.1.
PIRiB55123.
RefSeqiNP_011378.1. NM_001181002.1.

Genome annotation databases

EnsemblFungiiYGL137W; YGL137W; YGL137W.
GeneIDi852740.
KEGGisce:YGL137W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U11237 Genomic DNA. Translation: AAA61711.1 .
X92670 Genomic DNA. Translation: CAA63359.1 .
Z72659 Genomic DNA. Translation: CAA96848.1 .
BK006941 Genomic DNA. Translation: DAA07973.1 .
PIRi B55123.
RefSeqi NP_011378.1. NM_001181002.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YNN X-ray 1.78 A 1-304 [» ]
2YNO X-ray 1.80 A/B 1-304 [» ]
2YNP X-ray 2.96 A 1-604 [» ]
4J73 X-ray 1.44 A 1-301 [» ]
4J77 X-ray 1.76 A/B 1-301 [» ]
4J78 X-ray 1.48 A 1-301 [» ]
4J79 X-ray 1.56 A 1-300 [» ]
4J81 X-ray 1.74 A/B 1-301 [» ]
4J82 X-ray 1.46 A/B 1-301 [» ]
4J84 X-ray 1.47 A/B 1-301 [» ]
4J86 X-ray 1.48 A/B 1-301 [» ]
ProteinModelPortali P41811.
SMRi P41811. Positions 1-812.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33115. 246 interactions.
DIPi DIP-6468N.
IntActi P41811. 70 interactions.
MINTi MINT-428887.
STRINGi 4932.YGL137W.

Proteomic databases

MaxQBi P41811.
PaxDbi P41811.
PeptideAtlasi P41811.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL137W ; YGL137W ; YGL137W .
GeneIDi 852740.
KEGGi sce:YGL137W.

Organism-specific databases

CYGDi YGL137w.
SGDi S000003105. SEC27.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00740000115578.
HOGENOMi HOG000163444.
KOi K17302.
OMAi IAHNPNG.
OrthoDBi EOG7TN016.

Enzyme and pathway databases

BioCyci YEAST:G3O-30632-MONOMER.
Reactomei REACT_33101. COPI Mediated Transport.

Miscellaneous databases

NextBioi 972153.
PROi P41811.

Gene expression databases

Genevestigatori P41811.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
InterProi IPR006692. Coatomer_WD-assoc_reg.
IPR016453. COPB2.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF04053. Coatomer_WDAD. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view ]
PIRSFi PIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 4 hits.
PROSITEi PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic."
    Duden R., Hosobuchi M., Hamamoto S., Winey M., Byers B., Schekman R.
    J. Biol. Chem. 269:24486-24495(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-11.
    Strain: RSY255.
  2. "Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames."
    Escribano V., Eraso P., Portillo F., Mazon M.J.
    Yeast 12:887-892(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Yeast coatomer contains a subunit homologous to mammalian beta'-COP."
    Harter C., Draken E., Lottspeich F., Wieland F.T.
    FEBS Lett. 332:71-73(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-27.
    Strain: ATCC 208279 / BJ926.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast."
    Gabriely G., Kama R., Gerst J.E.
    Mol. Cell. Biol. 27:526-540(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS27.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCOPB2_YEAST
AccessioniPrimary (citable) accession number: P41811
Secondary accession number(s): D6VU12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 129000 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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