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P41809

- HKR1_YEAST

UniProt

P41809 - HKR1_YEAST

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Protein

Signaling mucin HKR1

Gene

HKR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plasma membrane signaling mucin that promotes activation of the MAPK for the filamentous growth pathway. May regulate beta-glucan synthesis. Overexpression provides resistance to HM-1 killer toxin.2 Publications

GO - Molecular functioni

  1. osmosensor activity Source: SGD

GO - Biological processi

  1. (1->3)-beta-D-glucan biosynthetic process Source: SGD
  2. cellular bud site selection Source: SGD
  3. fungal-type cell wall organization Source: SGD
  4. hyperosmotic response Source: SGD
  5. osmosensory signaling pathway via Sho1 osmosensor Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29961-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signaling mucin HKR1
Alternative name(s):
Hansenula MRAKII killer toxin-resistant protein 1
Gene namesi
Name:HKR1
Ordered Locus Names:YDR420W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR420w.
SGDiS000002828. HKR1.

Subcellular locationi

Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 14851464ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1486 – 150621HelicalSequence AnalysisAdd
BLAST
Topological domaini1507 – 1802296CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
  3. site of polarized growth Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 18021781Signaling mucin HKR1PRO_0000021444Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1252 – 12521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1293 – 12931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1342 – 13421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1400 – 14001N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Could be O-glycosylated in the serine/threonine-rich domain.

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

Expression is highly increased during spheroplast regeneration.1 Publication

Gene expression databases

GenevestigatoriP41809.

Interactioni

Protein-protein interaction databases

BioGridi32479. 73 interactions.
IntActiP41809. 3 interactions.
MINTiMINT-5223587.
STRINGi4932.YDR420W.

Structurei

3D structure databases

ProteinModelPortaliP41809.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati453 – 480281; approximateAdd
BLAST
Repeati481 – 508282Add
BLAST
Repeati509 – 536283Add
BLAST
Repeati537 – 564284Add
BLAST
Repeati565 – 592285Add
BLAST
Repeati593 – 620286Add
BLAST
Repeati621 – 648287Add
BLAST
Repeati649 – 676288Add
BLAST
Repeati677 – 704289Add
BLAST
Repeati705 – 7322810Add
BLAST
Repeati733 – 7602811Add
BLAST
Repeati761 – 7882812Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni453 – 78833612 X 28 AA tandem repeats of S-[AV]-[P]-V-A-V-S-S-T-Y-T-S-S-P-S-A-P-A-A-I-S-S-T-Y-T-S-S-PAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 14781456Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the HKR1/MSB2 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00770000121599.
InParanoidiP41809.
OMAiYHESEVE.
OrthoDBiEOG78H456.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41809-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVSLKIKKIL LLVSLLNAIE AYSNDTIYST SYNNGIESTP SYSTSAISST
60 70 80 90 100
GSSNKENAIT SSSETTTMAG QYGESGSTTI MDEQETGTSS QYISVTTTTQ
110 120 130 140 150
TSDTMSSVKK STEIATPSSS IVPTPLQSYS DESQISQTLS HNPKSVAESD
160 170 180 190 200
SDTTSSESSS SVIISTSDSS AVPREISPII TTDSQISKEE GTLAQTSSIS
210 220 230 240 250
ETTRIAQMVT RVSQISSITA ASTIDGFSSE STQTDFSNTV SFENSVEEEY
260 270 280 290 300
AMSKSQLSES YSSSSTVYSG GESTADKTSS SPITSFSSSY SQTTSTETSE
310 320 330 340 350
SSRVAVGVSR PSSITQTTSI DSFSMSEVEL STYYDLSAGN YPDQELIVDR
360 370 380 390 400
PATSSTAETS SEASQGVSRE SNTFAVSSIS TTNFIVSSAS DTVVSTSSTN
410 420 430 440 450
TVPYSSVHST FVHATSSSTY ISSSLYSSPS LSASVSSHFG VAPFPSAYIS
460 470 480 490 500
FSSVPVAVSS TYTSSPSASV VVPSAYASSP SVPVAVSSTY TSSPSAPAAI
510 520 530 540 550
SSTYTSSPSA PVAVSSTYTS SPSAPAAISS TYTSSPSAPV AVSSTYTSSP
560 570 580 590 600
SAPAAISSTY TSSPSAPVAV SSTYTSSPSA PVAISSTYTS SPSVPVAVSS
610 620 630 640 650
TYTSSPSAPA AISSTYTSSP SAPVAVSSTY TSSPSAPAAI SSTYTSSPSV
660 670 680 690 700
PVAVSSTYTS SPSAPAAISS TYTSSPSVPV AVSSTYTSSP SAPAAISSTY
710 720 730 740 750
TSSPSAPVAV SSTYTSSPSA PAAISSTYTS SPSAPVAVSS TYTSSPSAPA
760 770 780 790 800
AISSTYTSSP SAPVAVSSTY TSSPSALVVL SSTSTSSPYD IVYSPSTFAA
810 820 830 840 850
ISSGYTPSPS ASVAMSSTSS SSPYDIVYSL SSSASRSSIA TYEFSPSPST
860 870 880 890 900
SLPTSSTYTY FSSAYAFEFS SERYSTTSTI APTQIHSTLS RITDFLLQTS
910 920 930 940 950
MAIQSIVSQQ ISTSSTLNDE IHSSALSVFN PSASNLVETS LIISSTQASI
960 970 980 990 1000
TSPKNSAKIS SLQSQLSSST KNPYDTANKN TETSGRSTVV SNFLYTSSAA
1010 1020 1030 1040 1050
KPDNEKFSAT PTEITTISSS SHAYSLSIPS SHNSVTGLSH NFVDSSKSAT
1060 1070 1080 1090 1100
SFGYSSSSIS SIKLSKETIP ASKSVSNTQE RITSFTSTLR ANSQSEKSEG
1110 1120 1130 1140 1150
RNSVGSLQSS HISSNPSLST NTKVDSKSLS RKVSKTMGEN GEETGLTTTK
1160 1170 1180 1190 1200
TQYKSSSETS GSYSRSFTKI SIGPATTAVQ TQASTNSVFT APALSTYPTT
1210 1220 1230 1240 1250
PYPSPNSYAW LPTAIIVESS ETGPTTASFN PSITGSLPNA IEPAVAVSEP
1260 1270 1280 1290 1300
INHTLITIGF TAALNYVFLV QNPLSSAQIF NFLPLVLKYP FSNTSSELDN
1310 1320 1330 1340 1350
SIGELSTFIL SYRSGSSTTT LSPKSISSLS VVKKKKNQQK KNATKSTEDL
1360 1370 1380 1390 1400
HPPQVDTSSI AVKKIVPMVD SSKAYIVSVA EVYFPTEAVT YLQQLILDEN
1410 1420 1430 1440 1450
STLYSNPQTP LRSLAGLIDS GIPLGGLTLY GSGDGGYVPS LTSSSVLDSS
1460 1470 1480 1490 1500
KGNSQNIDGT YKYGALDDFI NSFTDSASAG KYAVKIIIFL IVLTIGVLLW
1510 1520 1530 1540 1550
LFVAFFAFRH RNILLKRHPR NCIGKSLNNE RELESTELSR SSSGNQVYNE
1560 1570 1580 1590 1600
KPPESENESV YSAVDDHYIV TGENTVYNTI HRLHYTINDD GDLLYRDAIP
1610 1620 1630 1640 1650
LDFDQTNGDD GSGIDSIVRD CVYDKNQDAT EAFLNDEESI SGILDVDENG
1660 1670 1680 1690 1700
DIRLYDSYSD NEESNSFHLP DEVIENYNKN HLCETKLHGL GTESCTTDDP
1710 1720 1730 1740 1750
DTGNQITNEF STGSQTCLPS TAYTTPLHTN SIKLHTLRYT ESSLPKPNQT
1760 1770 1780 1790 1800
LFSNLEDLEI EDIDDNGSVS DVHIEELDAL DEELYKRMSK VIKQQNHQTT

KI
Length:1,802
Mass (Da):188,948
Last modified:August 31, 2004 - v2
Checksum:i0FF726990C7CDC51
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti582 – 5821V → A in AAB30051. (PubMed:8113191)Curated
Sequence conflicti594 – 5941V → A in AAB30051. (PubMed:8113191)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69101 Genomic DNA. Translation: AAB30051.1.
U33007 Genomic DNA. Translation: AAB64857.1.
BK006938 Genomic DNA. Translation: DAA12260.1.
PIRiS69703.
RefSeqiNP_010708.1. NM_001180728.1.

Genome annotation databases

EnsemblFungiiYDR420W; YDR420W; YDR420W.
GeneIDi852030.
KEGGisce:YDR420W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69101 Genomic DNA. Translation: AAB30051.1 .
U33007 Genomic DNA. Translation: AAB64857.1 .
BK006938 Genomic DNA. Translation: DAA12260.1 .
PIRi S69703.
RefSeqi NP_010708.1. NM_001180728.1.

3D structure databases

ProteinModelPortali P41809.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32479. 73 interactions.
IntActi P41809. 3 interactions.
MINTi MINT-5223587.
STRINGi 4932.YDR420W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR420W ; YDR420W ; YDR420W .
GeneIDi 852030.
KEGGi sce:YDR420W.

Organism-specific databases

CYGDi YDR420w.
SGDi S000002828. HKR1.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00770000121599.
InParanoidi P41809.
OMAi YHESEVE.
OrthoDBi EOG78H456.

Enzyme and pathway databases

BioCyci YEAST:G3O-29961-MONOMER.

Miscellaneous databases

NextBioi 970260.

Gene expression databases

Genevestigatori P41809.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the Saccharomyces cerevisiae gene whose overexpression overcomes the effects of HM-1 killer toxin, which inhibits beta-glucan synthesis."
    Kasahara S., Yamada H., Mio T., Shiratori Y., Miyamoto C., Yabe T., Nakajima T., Ichishima E., Furuichi Y.
    J. Bacteriol. 176:1488-1499(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 200358 / YNN 295.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "HKR1 encodes a cell surface protein that regulates both cell wall beta-glucan synthesis and budding pattern in the yeast Saccharomyces cerevisiae."
    Yabe T., Yamada-Okabe T., Kasahara S., Furuichi Y., Nakajima T., Ichishima E., Arisawa M., Yamada-Okabe H.
    J. Bacteriol. 178:477-483(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Variations in mRNA transcript levels of cell wall-associated genes of Saccharomyces cerevisiae following spheroplasting."
    Braley R., Chaffin W.L.
    FEMS Microbiol. Lett. 181:177-185(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response."
    Pitoniak A., Birkaya B., Dionne H.M., Vadaie N., Cullen P.J.
    Mol. Biol. Cell 20:3101-3114(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHKR1_YEAST
AccessioniPrimary (citable) accession number: P41809
Secondary accession number(s): D6VT50, Q04051
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 31, 2004
Last modified: November 26, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3