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P41809

- HKR1_YEAST

UniProt

P41809 - HKR1_YEAST

Protein

Signaling mucin HKR1

Gene

HKR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    Plasma membrane signaling mucin that promotes activation of the MAPK for the filamentous growth pathway. May regulate beta-glucan synthesis. Overexpression provides resistance to HM-1 killer toxin.2 Publications

    GO - Molecular functioni

    1. osmosensor activity Source: SGD

    GO - Biological processi

    1. (1->3)-beta-D-glucan biosynthetic process Source: SGD
    2. cellular bud site selection Source: SGD
    3. fungal-type cell wall organization Source: SGD
    4. hyperosmotic response Source: SGD
    5. osmosensory signaling pathway via Sho1 osmosensor Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29961-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signaling mucin HKR1
    Alternative name(s):
    Hansenula MRAKII killer toxin-resistant protein 1
    Gene namesi
    Name:HKR1
    Ordered Locus Names:YDR420W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR420w.
    SGDiS000002828. HKR1.

    Subcellular locationi

    Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD
    3. site of polarized growth Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 18021781Signaling mucin HKR1PRO_0000021444Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1252 – 12521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1293 – 12931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1342 – 13421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1400 – 14001N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Could be O-glycosylated in the serine/threonine-rich domain.

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Inductioni

    Expression is highly increased during spheroplast regeneration.1 Publication

    Gene expression databases

    GenevestigatoriP41809.

    Interactioni

    Protein-protein interaction databases

    BioGridi32479. 73 interactions.
    IntActiP41809. 3 interactions.
    MINTiMINT-5223587.
    STRINGi4932.YDR420W.

    Structurei

    3D structure databases

    ProteinModelPortaliP41809.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 14851464ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1507 – 1802296CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1486 – 150621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati453 – 480281; approximateAdd
    BLAST
    Repeati481 – 508282Add
    BLAST
    Repeati509 – 536283Add
    BLAST
    Repeati537 – 564284Add
    BLAST
    Repeati565 – 592285Add
    BLAST
    Repeati593 – 620286Add
    BLAST
    Repeati621 – 648287Add
    BLAST
    Repeati649 – 676288Add
    BLAST
    Repeati677 – 704289Add
    BLAST
    Repeati705 – 7322810Add
    BLAST
    Repeati733 – 7602811Add
    BLAST
    Repeati761 – 7882812Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni453 – 78833612 X 28 AA tandem repeats of S-[AV]-[P]-V-A-V-S-S-T-Y-T-S-S-P-S-A-P-A-A-I-S-S-T-Y-T-S-S-PAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi23 – 14781456Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HKR1/MSB2 family.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00740000116199.
    OMAiYHESEVE.
    OrthoDBiEOG78H456.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P41809-1 [UniParc]FASTAAdd to Basket

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    MVSLKIKKIL LLVSLLNAIE AYSNDTIYST SYNNGIESTP SYSTSAISST     50
    GSSNKENAIT SSSETTTMAG QYGESGSTTI MDEQETGTSS QYISVTTTTQ 100
    TSDTMSSVKK STEIATPSSS IVPTPLQSYS DESQISQTLS HNPKSVAESD 150
    SDTTSSESSS SVIISTSDSS AVPREISPII TTDSQISKEE GTLAQTSSIS 200
    ETTRIAQMVT RVSQISSITA ASTIDGFSSE STQTDFSNTV SFENSVEEEY 250
    AMSKSQLSES YSSSSTVYSG GESTADKTSS SPITSFSSSY SQTTSTETSE 300
    SSRVAVGVSR PSSITQTTSI DSFSMSEVEL STYYDLSAGN YPDQELIVDR 350
    PATSSTAETS SEASQGVSRE SNTFAVSSIS TTNFIVSSAS DTVVSTSSTN 400
    TVPYSSVHST FVHATSSSTY ISSSLYSSPS LSASVSSHFG VAPFPSAYIS 450
    FSSVPVAVSS TYTSSPSASV VVPSAYASSP SVPVAVSSTY TSSPSAPAAI 500
    SSTYTSSPSA PVAVSSTYTS SPSAPAAISS TYTSSPSAPV AVSSTYTSSP 550
    SAPAAISSTY TSSPSAPVAV SSTYTSSPSA PVAISSTYTS SPSVPVAVSS 600
    TYTSSPSAPA AISSTYTSSP SAPVAVSSTY TSSPSAPAAI SSTYTSSPSV 650
    PVAVSSTYTS SPSAPAAISS TYTSSPSVPV AVSSTYTSSP SAPAAISSTY 700
    TSSPSAPVAV SSTYTSSPSA PAAISSTYTS SPSAPVAVSS TYTSSPSAPA 750
    AISSTYTSSP SAPVAVSSTY TSSPSALVVL SSTSTSSPYD IVYSPSTFAA 800
    ISSGYTPSPS ASVAMSSTSS SSPYDIVYSL SSSASRSSIA TYEFSPSPST 850
    SLPTSSTYTY FSSAYAFEFS SERYSTTSTI APTQIHSTLS RITDFLLQTS 900
    MAIQSIVSQQ ISTSSTLNDE IHSSALSVFN PSASNLVETS LIISSTQASI 950
    TSPKNSAKIS SLQSQLSSST KNPYDTANKN TETSGRSTVV SNFLYTSSAA 1000
    KPDNEKFSAT PTEITTISSS SHAYSLSIPS SHNSVTGLSH NFVDSSKSAT 1050
    SFGYSSSSIS SIKLSKETIP ASKSVSNTQE RITSFTSTLR ANSQSEKSEG 1100
    RNSVGSLQSS HISSNPSLST NTKVDSKSLS RKVSKTMGEN GEETGLTTTK 1150
    TQYKSSSETS GSYSRSFTKI SIGPATTAVQ TQASTNSVFT APALSTYPTT 1200
    PYPSPNSYAW LPTAIIVESS ETGPTTASFN PSITGSLPNA IEPAVAVSEP 1250
    INHTLITIGF TAALNYVFLV QNPLSSAQIF NFLPLVLKYP FSNTSSELDN 1300
    SIGELSTFIL SYRSGSSTTT LSPKSISSLS VVKKKKNQQK KNATKSTEDL 1350
    HPPQVDTSSI AVKKIVPMVD SSKAYIVSVA EVYFPTEAVT YLQQLILDEN 1400
    STLYSNPQTP LRSLAGLIDS GIPLGGLTLY GSGDGGYVPS LTSSSVLDSS 1450
    KGNSQNIDGT YKYGALDDFI NSFTDSASAG KYAVKIIIFL IVLTIGVLLW 1500
    LFVAFFAFRH RNILLKRHPR NCIGKSLNNE RELESTELSR SSSGNQVYNE 1550
    KPPESENESV YSAVDDHYIV TGENTVYNTI HRLHYTINDD GDLLYRDAIP 1600
    LDFDQTNGDD GSGIDSIVRD CVYDKNQDAT EAFLNDEESI SGILDVDENG 1650
    DIRLYDSYSD NEESNSFHLP DEVIENYNKN HLCETKLHGL GTESCTTDDP 1700
    DTGNQITNEF STGSQTCLPS TAYTTPLHTN SIKLHTLRYT ESSLPKPNQT 1750
    LFSNLEDLEI EDIDDNGSVS DVHIEELDAL DEELYKRMSK VIKQQNHQTT 1800
    KI 1802
    Length:1,802
    Mass (Da):188,948
    Last modified:August 31, 2004 - v2
    Checksum:i0FF726990C7CDC51
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti582 – 5821V → A in AAB30051. (PubMed:8113191)Curated
    Sequence conflicti594 – 5941V → A in AAB30051. (PubMed:8113191)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69101 Genomic DNA. Translation: AAB30051.1.
    U33007 Genomic DNA. Translation: AAB64857.1.
    BK006938 Genomic DNA. Translation: DAA12260.1.
    PIRiS69703.
    RefSeqiNP_010708.1. NM_001180728.1.

    Genome annotation databases

    EnsemblFungiiYDR420W; YDR420W; YDR420W.
    GeneIDi852030.
    KEGGisce:YDR420W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S69101 Genomic DNA. Translation: AAB30051.1 .
    U33007 Genomic DNA. Translation: AAB64857.1 .
    BK006938 Genomic DNA. Translation: DAA12260.1 .
    PIRi S69703.
    RefSeqi NP_010708.1. NM_001180728.1.

    3D structure databases

    ProteinModelPortali P41809.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32479. 73 interactions.
    IntActi P41809. 3 interactions.
    MINTi MINT-5223587.
    STRINGi 4932.YDR420W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR420W ; YDR420W ; YDR420W .
    GeneIDi 852030.
    KEGGi sce:YDR420W.

    Organism-specific databases

    CYGDi YDR420w.
    SGDi S000002828. HKR1.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00740000116199.
    OMAi YHESEVE.
    OrthoDBi EOG78H456.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29961-MONOMER.

    Miscellaneous databases

    NextBioi 970260.

    Gene expression databases

    Genevestigatori P41809.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the Saccharomyces cerevisiae gene whose overexpression overcomes the effects of HM-1 killer toxin, which inhibits beta-glucan synthesis."
      Kasahara S., Yamada H., Mio T., Shiratori Y., Miyamoto C., Yabe T., Nakajima T., Ichishima E., Furuichi Y.
      J. Bacteriol. 176:1488-1499(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 200358 / YNN 295.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "HKR1 encodes a cell surface protein that regulates both cell wall beta-glucan synthesis and budding pattern in the yeast Saccharomyces cerevisiae."
      Yabe T., Yamada-Okabe T., Kasahara S., Furuichi Y., Nakajima T., Ichishima E., Arisawa M., Yamada-Okabe H.
      J. Bacteriol. 178:477-483(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. "Variations in mRNA transcript levels of cell wall-associated genes of Saccharomyces cerevisiae following spheroplasting."
      Braley R., Chaffin W.L.
      FEMS Microbiol. Lett. 181:177-185(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response."
      Pitoniak A., Birkaya B., Dionne H.M., Vadaie N., Cullen P.J.
      Mol. Biol. Cell 20:3101-3114(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHKR1_YEAST
    AccessioniPrimary (citable) accession number: P41809
    Secondary accession number(s): D6VT50, Q04051
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3