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P41809 (HKR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signaling mucin HKR1
Alternative name(s):
Hansenula MRAKII killer toxin-resistant protein 1
Gene names
Name:HKR1
Ordered Locus Names:YDR420W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1802 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plasma membrane signaling mucin that promotes activation of the MAPK for the filamentous growth pathway. May regulate beta-glucan synthesis. Overexpression provides resistance to HM-1 killer toxin. Ref.4 Ref.6

Subcellular location

Cell membrane; Single-pass type I membrane protein Ref.4.

Induction

Expression is highly increased during spheroplast regeneration. Ref.5

Post-translational modification

Could be O-glycosylated in the serine/threonine-rich domain.

Sequence similarities

Belongs to the HKR1/MSB2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 18021781Signaling mucin HKR1
PRO_0000021444

Regions

Topological domain22 – 14851464Extracellular Potential
Transmembrane1486 – 150621Helical; Potential
Topological domain1507 – 1802296Cytoplasmic Potential
Repeat453 – 480281; approximate
Repeat481 – 508282
Repeat509 – 536283
Repeat537 – 564284
Repeat565 – 592285
Repeat593 – 620286
Repeat621 – 648287
Repeat649 – 676288
Repeat677 – 704289
Repeat705 – 7322810
Repeat733 – 7602811
Repeat761 – 7882812
Region453 – 78833612 X 28 AA tandem repeats of S-[AV]-[P]-V-A-V-S-S-T-Y-T-S-S-P-S-A-P-A-A-I-S-S-T-Y-T-S-S-P
Compositional bias23 – 14781456Ser/Thr-rich

Amino acid modifications

Glycosylation241N-linked (GlcNAc...) Potential
Glycosylation12521N-linked (GlcNAc...) Potential
Glycosylation12931N-linked (GlcNAc...) Potential
Glycosylation13421N-linked (GlcNAc...) Potential
Glycosylation14001N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5821V → A in AAB30051. Ref.1
Sequence conflict5941V → A in AAB30051. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P41809 [UniParc].

Last modified August 31, 2004. Version 2.
Checksum: 0FF726990C7CDC51

FASTA1,802188,948
        10         20         30         40         50         60 
MVSLKIKKIL LLVSLLNAIE AYSNDTIYST SYNNGIESTP SYSTSAISST GSSNKENAIT 

        70         80         90        100        110        120 
SSSETTTMAG QYGESGSTTI MDEQETGTSS QYISVTTTTQ TSDTMSSVKK STEIATPSSS 

       130        140        150        160        170        180 
IVPTPLQSYS DESQISQTLS HNPKSVAESD SDTTSSESSS SVIISTSDSS AVPREISPII 

       190        200        210        220        230        240 
TTDSQISKEE GTLAQTSSIS ETTRIAQMVT RVSQISSITA ASTIDGFSSE STQTDFSNTV 

       250        260        270        280        290        300 
SFENSVEEEY AMSKSQLSES YSSSSTVYSG GESTADKTSS SPITSFSSSY SQTTSTETSE 

       310        320        330        340        350        360 
SSRVAVGVSR PSSITQTTSI DSFSMSEVEL STYYDLSAGN YPDQELIVDR PATSSTAETS 

       370        380        390        400        410        420 
SEASQGVSRE SNTFAVSSIS TTNFIVSSAS DTVVSTSSTN TVPYSSVHST FVHATSSSTY 

       430        440        450        460        470        480 
ISSSLYSSPS LSASVSSHFG VAPFPSAYIS FSSVPVAVSS TYTSSPSASV VVPSAYASSP 

       490        500        510        520        530        540 
SVPVAVSSTY TSSPSAPAAI SSTYTSSPSA PVAVSSTYTS SPSAPAAISS TYTSSPSAPV 

       550        560        570        580        590        600 
AVSSTYTSSP SAPAAISSTY TSSPSAPVAV SSTYTSSPSA PVAISSTYTS SPSVPVAVSS 

       610        620        630        640        650        660 
TYTSSPSAPA AISSTYTSSP SAPVAVSSTY TSSPSAPAAI SSTYTSSPSV PVAVSSTYTS 

       670        680        690        700        710        720 
SPSAPAAISS TYTSSPSVPV AVSSTYTSSP SAPAAISSTY TSSPSAPVAV SSTYTSSPSA 

       730        740        750        760        770        780 
PAAISSTYTS SPSAPVAVSS TYTSSPSAPA AISSTYTSSP SAPVAVSSTY TSSPSALVVL 

       790        800        810        820        830        840 
SSTSTSSPYD IVYSPSTFAA ISSGYTPSPS ASVAMSSTSS SSPYDIVYSL SSSASRSSIA 

       850        860        870        880        890        900 
TYEFSPSPST SLPTSSTYTY FSSAYAFEFS SERYSTTSTI APTQIHSTLS RITDFLLQTS 

       910        920        930        940        950        960 
MAIQSIVSQQ ISTSSTLNDE IHSSALSVFN PSASNLVETS LIISSTQASI TSPKNSAKIS 

       970        980        990       1000       1010       1020 
SLQSQLSSST KNPYDTANKN TETSGRSTVV SNFLYTSSAA KPDNEKFSAT PTEITTISSS 

      1030       1040       1050       1060       1070       1080 
SHAYSLSIPS SHNSVTGLSH NFVDSSKSAT SFGYSSSSIS SIKLSKETIP ASKSVSNTQE 

      1090       1100       1110       1120       1130       1140 
RITSFTSTLR ANSQSEKSEG RNSVGSLQSS HISSNPSLST NTKVDSKSLS RKVSKTMGEN 

      1150       1160       1170       1180       1190       1200 
GEETGLTTTK TQYKSSSETS GSYSRSFTKI SIGPATTAVQ TQASTNSVFT APALSTYPTT 

      1210       1220       1230       1240       1250       1260 
PYPSPNSYAW LPTAIIVESS ETGPTTASFN PSITGSLPNA IEPAVAVSEP INHTLITIGF 

      1270       1280       1290       1300       1310       1320 
TAALNYVFLV QNPLSSAQIF NFLPLVLKYP FSNTSSELDN SIGELSTFIL SYRSGSSTTT 

      1330       1340       1350       1360       1370       1380 
LSPKSISSLS VVKKKKNQQK KNATKSTEDL HPPQVDTSSI AVKKIVPMVD SSKAYIVSVA 

      1390       1400       1410       1420       1430       1440 
EVYFPTEAVT YLQQLILDEN STLYSNPQTP LRSLAGLIDS GIPLGGLTLY GSGDGGYVPS 

      1450       1460       1470       1480       1490       1500 
LTSSSVLDSS KGNSQNIDGT YKYGALDDFI NSFTDSASAG KYAVKIIIFL IVLTIGVLLW 

      1510       1520       1530       1540       1550       1560 
LFVAFFAFRH RNILLKRHPR NCIGKSLNNE RELESTELSR SSSGNQVYNE KPPESENESV 

      1570       1580       1590       1600       1610       1620 
YSAVDDHYIV TGENTVYNTI HRLHYTINDD GDLLYRDAIP LDFDQTNGDD GSGIDSIVRD 

      1630       1640       1650       1660       1670       1680 
CVYDKNQDAT EAFLNDEESI SGILDVDENG DIRLYDSYSD NEESNSFHLP DEVIENYNKN 

      1690       1700       1710       1720       1730       1740 
HLCETKLHGL GTESCTTDDP DTGNQITNEF STGSQTCLPS TAYTTPLHTN SIKLHTLRYT 

      1750       1760       1770       1780       1790       1800 
ESSLPKPNQT LFSNLEDLEI EDIDDNGSVS DVHIEELDAL DEELYKRMSK VIKQQNHQTT 


KI 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the Saccharomyces cerevisiae gene whose overexpression overcomes the effects of HM-1 killer toxin, which inhibits beta-glucan synthesis."
Kasahara S., Yamada H., Mio T., Shiratori Y., Miyamoto C., Yabe T., Nakajima T., Ichishima E., Furuichi Y.
J. Bacteriol. 176:1488-1499(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 200358 / YNN 295.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"HKR1 encodes a cell surface protein that regulates both cell wall beta-glucan synthesis and budding pattern in the yeast Saccharomyces cerevisiae."
Yabe T., Yamada-Okabe T., Kasahara S., Furuichi Y., Nakajima T., Ichishima E., Arisawa M., Yamada-Okabe H.
J. Bacteriol. 178:477-483(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Variations in mRNA transcript levels of cell wall-associated genes of Saccharomyces cerevisiae following spheroplasting."
Braley R., Chaffin W.L.
FEMS Microbiol. Lett. 181:177-185(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response."
Pitoniak A., Birkaya B., Dionne H.M., Vadaie N., Cullen P.J.
Mol. Biol. Cell 20:3101-3114(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S69101 Genomic DNA. Translation: AAB30051.1.
U33007 Genomic DNA. Translation: AAB64857.1.
BK006938 Genomic DNA. Translation: DAA12260.1.
PIRS69703.
RefSeqNP_010708.1. NM_001180728.1.

3D structure databases

ProteinModelPortalP41809.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32479. 72 interactions.
IntActP41809. 3 interactions.
MINTMINT-5223587.
STRING4932.YDR420W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR420W; YDR420W; YDR420W.
GeneID852030.
KEGGsce:YDR420W.

Organism-specific databases

CYGDYDR420w.
SGDS000002828. HKR1.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00740000116199.
OMAHESEVES.
OrthoDBEOG78H456.

Enzyme and pathway databases

BioCycYEAST:G3O-29961-MONOMER.

Gene expression databases

GenevestigatorP41809.

Family and domain databases

ProtoNetSearch...

Other

NextBio970260.

Entry information

Entry nameHKR1_YEAST
AccessionPrimary (citable) accession number: P41809
Secondary accession number(s): D6VT50, Q04051
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 31, 2004
Last modified: February 19, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families