P41809 (HKR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signaling mucin HKR1

Alternative name(s):
Hansenula MRAKII killer toxin-resistant protein 1

Gene names

Name:	HKR1
Ordered Locus Names:	YDR420W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1802 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plasma membrane signaling mucin that promotes activation of the MAPK for the filamentous growth pathway. May regulate beta-glucan synthesis. Overexpression provides resistance to HM-1 killer toxin. Ref.4 Ref.6
Subcellular location	Cell membrane; Single-pass type I membrane protein Ref.4.
Induction	Expression is highly increased during spheroplast regeneration. Ref.5
Post-translational modification	Could be O-glycosylated in the serine/threonine-rich domain.
Sequence similarities	Belongs to the HKR1/MSB2 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	(1->3)-beta-D-glucan biosynthetic process Inferred from mutant phenotype Ref.4. Source: SGD cellular bud site selection Inferred from mutant phenotype Ref.4. Source: SGD fungal-type cell wall organization Inferred from mutant phenotype Ref.4. Source: SGD hyperosmotic response Inferred from genetic interaction PubMed 17627274. Source: SGD osmosensory signaling pathway via Sho1 osmosensor Inferred from genetic interaction PubMed 17627274. Source: SGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.4. Source: SGD site of polarized growth Inferred from direct assay PubMed 17627274. Source: SGD
Molecular_function	osmosensor activity Inferred from genetic interaction PubMed 17627274. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 1802	1781	Signaling mucin HKR1		PRO_0000021444
Regions
	Topological domain	22 – 1485	1464	Extracellular Potential
	Transmembrane	1486 – 1506	21	Helical; Potential
	Topological domain	1507 – 1802	296	Cytoplasmic Potential
	Repeat	453 – 480	28	1; approximate
	Repeat	481 – 508	28	2
	Repeat	509 – 536	28	3
	Repeat	537 – 564	28	4
	Repeat	565 – 592	28	5
	Repeat	593 – 620	28	6
	Repeat	621 – 648	28	7
	Repeat	649 – 676	28	8
	Repeat	677 – 704	28	9
	Repeat	705 – 732	28	10
	Repeat	733 – 760	28	11
	Repeat	761 – 788	28	12
	Region	453 – 788	336	12 X 28 AA tandem repeats of S-[AV]-[P]-V-A-V-S-S-T-Y-T-S-S-P-S-A-P-A-A-I-S-S-T-Y-T-S-S-P
	Compositional bias	23 – 1478	1456	Ser/Thr-rich
Amino acid modifications
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	1252	1	N-linked (GlcNAc...) Potential
	Glycosylation	1293	1	N-linked (GlcNAc...) Potential
	Glycosylation	1342	1	N-linked (GlcNAc...) Potential
	Glycosylation	1400	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	582	1	V → A in AAB30051. Ref.1
	Sequence conflict	594	1	V → A in AAB30051. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P41809 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 0FF726990C7CDC51	FASTA	1,802	188,948
	10 20 30 40 50 60 MVSLKIKKIL LLVSLLNAIE AYSNDTIYST SYNNGIESTP SYSTSAISST GSSNKENAIT 70 80 90 100 110 120 SSSETTTMAG QYGESGSTTI MDEQETGTSS QYISVTTTTQ TSDTMSSVKK STEIATPSSS 130 140 150 160 170 180 IVPTPLQSYS DESQISQTLS HNPKSVAESD SDTTSSESSS SVIISTSDSS AVPREISPII 190 200 210 220 230 240 TTDSQISKEE GTLAQTSSIS ETTRIAQMVT RVSQISSITA ASTIDGFSSE STQTDFSNTV 250 260 270 280 290 300 SFENSVEEEY AMSKSQLSES YSSSSTVYSG GESTADKTSS SPITSFSSSY SQTTSTETSE 310 320 330 340 350 360 SSRVAVGVSR PSSITQTTSI DSFSMSEVEL STYYDLSAGN YPDQELIVDR PATSSTAETS 370 380 390 400 410 420 SEASQGVSRE SNTFAVSSIS TTNFIVSSAS DTVVSTSSTN TVPYSSVHST FVHATSSSTY 430 440 450 460 470 480 ISSSLYSSPS LSASVSSHFG VAPFPSAYIS FSSVPVAVSS TYTSSPSASV VVPSAYASSP 490 500 510 520 530 540 SVPVAVSSTY TSSPSAPAAI SSTYTSSPSA PVAVSSTYTS SPSAPAAISS TYTSSPSAPV 550 560 570 580 590 600 AVSSTYTSSP SAPAAISSTY TSSPSAPVAV SSTYTSSPSA PVAISSTYTS SPSVPVAVSS 610 620 630 640 650 660 TYTSSPSAPA AISSTYTSSP SAPVAVSSTY TSSPSAPAAI SSTYTSSPSV PVAVSSTYTS 670 680 690 700 710 720 SPSAPAAISS TYTSSPSVPV AVSSTYTSSP SAPAAISSTY TSSPSAPVAV SSTYTSSPSA 730 740 750 760 770 780 PAAISSTYTS SPSAPVAVSS TYTSSPSAPA AISSTYTSSP SAPVAVSSTY TSSPSALVVL 790 800 810 820 830 840 SSTSTSSPYD IVYSPSTFAA ISSGYTPSPS ASVAMSSTSS SSPYDIVYSL SSSASRSSIA 850 860 870 880 890 900 TYEFSPSPST SLPTSSTYTY FSSAYAFEFS SERYSTTSTI APTQIHSTLS RITDFLLQTS 910 920 930 940 950 960 MAIQSIVSQQ ISTSSTLNDE IHSSALSVFN PSASNLVETS LIISSTQASI TSPKNSAKIS 970 980 990 1000 1010 1020 SLQSQLSSST KNPYDTANKN TETSGRSTVV SNFLYTSSAA KPDNEKFSAT PTEITTISSS 1030 1040 1050 1060 1070 1080 SHAYSLSIPS SHNSVTGLSH NFVDSSKSAT SFGYSSSSIS SIKLSKETIP ASKSVSNTQE 1090 1100 1110 1120 1130 1140 RITSFTSTLR ANSQSEKSEG RNSVGSLQSS HISSNPSLST NTKVDSKSLS RKVSKTMGEN 1150 1160 1170 1180 1190 1200 GEETGLTTTK TQYKSSSETS GSYSRSFTKI SIGPATTAVQ TQASTNSVFT APALSTYPTT 1210 1220 1230 1240 1250 1260 PYPSPNSYAW LPTAIIVESS ETGPTTASFN PSITGSLPNA IEPAVAVSEP INHTLITIGF 1270 1280 1290 1300 1310 1320 TAALNYVFLV QNPLSSAQIF NFLPLVLKYP FSNTSSELDN SIGELSTFIL SYRSGSSTTT 1330 1340 1350 1360 1370 1380 LSPKSISSLS VVKKKKNQQK KNATKSTEDL HPPQVDTSSI AVKKIVPMVD SSKAYIVSVA 1390 1400 1410 1420 1430 1440 EVYFPTEAVT YLQQLILDEN STLYSNPQTP LRSLAGLIDS GIPLGGLTLY GSGDGGYVPS 1450 1460 1470 1480 1490 1500 LTSSSVLDSS KGNSQNIDGT YKYGALDDFI NSFTDSASAG KYAVKIIIFL IVLTIGVLLW 1510 1520 1530 1540 1550 1560 LFVAFFAFRH RNILLKRHPR NCIGKSLNNE RELESTELSR SSSGNQVYNE KPPESENESV 1570 1580 1590 1600 1610 1620 YSAVDDHYIV TGENTVYNTI HRLHYTINDD GDLLYRDAIP LDFDQTNGDD GSGIDSIVRD 1630 1640 1650 1660 1670 1680 CVYDKNQDAT EAFLNDEESI SGILDVDENG DIRLYDSYSD NEESNSFHLP DEVIENYNKN 1690 1700 1710 1720 1730 1740 HLCETKLHGL GTESCTTDDP DTGNQITNEF STGSQTCLPS TAYTTPLHTN SIKLHTLRYT 1750 1760 1770 1780 1790 1800 ESSLPKPNQT LFSNLEDLEI EDIDDNGSVS DVHIEELDAL DEELYKRMSK VIKQQNHQTT KI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the Saccharomyces cerevisiae gene whose overexpression overcomes the effects of HM-1 killer toxin, which inhibits beta-glucan synthesis." Kasahara S., Yamada H., Mio T., Shiratori Y., Miyamoto C., Yabe T., Nakajima T., Ichishima E., Furuichi Y. J. Bacteriol. 176:1488-1499(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 200358 / YNN 295.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"HKR1 encodes a cell surface protein that regulates both cell wall beta-glucan synthesis and budding pattern in the yeast Saccharomyces cerevisiae." Yabe T., Yamada-Okabe T., Kasahara S., Furuichi Y., Nakajima T., Ichishima E., Arisawa M., Yamada-Okabe H. J. Bacteriol. 178:477-483(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Variations in mRNA transcript levels of cell wall-associated genes of Saccharomyces cerevisiae following spheroplasting." Braley R., Chaffin W.L. FEMS Microbiol. Lett. 181:177-185(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response." Pitoniak A., Birkaya B., Dionne H.M., Vadaie N., Cullen P.J. Mol. Biol. Cell 20:3101-3114(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S69101 Genomic DNA. Translation: AAB30051.1. U33007 Genomic DNA. Translation: AAB64857.1. BK006938 Genomic DNA. Translation: DAA12260.1.
PIR	S69703.
RefSeq	NP_010708.1. NM_001180728.1.
3D structure databases
ProteinModelPortal	P41809.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41809. 2 interactions.
MINT	MINT-5223587.
STRING	4932.YDR420W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR420W; YDR420W; YDR420W.
GeneID	852030.
KEGG	sce:YDR420W.
Organism-specific databases
CYGD	YDR420w.
SGD	S000002828. HKR1.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00700000104744.
OMA	HESEVES.
OrthoDB	EOG4TTKTN.
Gene expression databases
Genevestigator	P41809.
GermOnline	YDR420W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	970260.

Entry information

Entry name

HKR1_YEAST

Accession

Primary (citable) accession number: P41809
Secondary accession number(s): D6VT50, Q04051

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	August 31, 2004
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program