P41808 (SMK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sporulation-specific mitogen-activated protein kinase SMK1
Short name=MAP kinase SMK1
EC=2.7.11.24

Gene names

Name:	SMK1
Ordered Locus Names:	YPR054W
ORF Names:	YP9499.10

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for spore wall assembly. Required for proper deposition of the two outer layers of the spore wall, the chitosan and dityrosine layers. Negatively regulates GSC2, an alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program. Ref.1 Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subunit structure	Interacts with GSC2. Ref.4
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-207 and Tyr-209, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Sporulation
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ascospore wall assembly Inferred from mutant phenotype Ref.4Ref.1. Source: SGD negative regulation of sporulation resulting in formation of a cellular spore Inferred from genetic interaction Ref.4. Source: SGD regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Sporulation-specific mitogen-activated protein kinase SMK1		PRO_0000186339
Regions
	Domain	38 – 337	300	Protein kinase
	Nucleotide binding	44 – 52	9	ATP By similarity
	Motif	207 – 209	3	TXY
	Compositional bias	384 – 387	4	Poly-Ser
Sites
	Active site	166	1	Proton acceptor By similarity
	Binding site	69	1	ATP By similarity
Amino acid modifications
	Modified residue	207	1	Phosphothreonine By similarity
	Modified residue	209	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41808 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 081BD00B9DBC3F23	FASTA	388	44,300
	10 20 30 40 50 60 MNCTLTDNTR AINVASNLGA PQQRTIFAKE RISIPGYYEI IQFLGKGAYG TVCSVKFKGR 70 80 90 100 110 120 SPAARIAVKK ISNIFNKEIL LKRAIRELKF MNFFKGHKNI VNLIDLEIVT SSPYDGLYCY 130 140 150 160 170 180 QELIDYDLAK VIHSSVQLSE FHIKYFLYQI LCGLKYIHSA DVIHRDLKPG NILCTLNGCL 190 200 210 220 230 240 KICDFGLARG IHAGFFKCHS TVQPHITNYV ATRWYRAPEL LLSNQPYSKS VDIWAVGCIL 250 260 270 280 290 300 AEFYARKPVF MGRDSMHQIF EIIKVLGTPD KDILIKFGTI KAWNLGKNSN NPVYKKIPWS 310 320 330 340 350 360 NIFPFASHEA INLIESLLHW DSTHRLNVEQ AISHPFLNEV RKPDDEPVCL QGPFDFTYES 370 380 ELNSMSKLRD YLVEEVKNFK TDLSSSSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae." Krisak L., Strich R., Winters R.S., Hall J.P., Mallory M.J., Kreitzer D., Tuan R.S., Winter E. Genes Dev. 8:2151-2161(1994) [PubMed: 7958885] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae." Huang L.S., Doherty H.K., Herskowitz I. Proc. Natl. Acad. Sci. U.S.A. 102:12431-12436(2005) [PubMed: 16116083] [Abstract] Cited for: FUNCTION, INTERACTION WITH GSC2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L35047 Genomic DNA. Translation: AAB59325.1. Z49219 Genomic DNA. Translation: CAA89172.1. Z71255 Genomic DNA. Translation: CAA94999.1. BK006949 Genomic DNA. Translation: DAA11476.1.
PIR	S48879.
RefSeq	NP_015379.1. NM_001184151.1.
3D structure databases
ProteinModelPortal	P41808.
SMR	P41808. Positions 29-381.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1636N.
IntAct	P41808. 14 interactions.
MINT	MINT-400914.
STRING	P41808.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR054W; YPR054W; YPR054W.
GeneID	856167.
KEGG	sce:YPR054W.
NMPDR	fig|4932.3.peg.6515.
Organism-specific databases
CYGD	YPR054w.
SGD	S000006258. SMK1.
Phylogenomic databases
eggNOG	fuNOG07907.
GeneTree	EFGT00050000000591.
HOGENOM	HBG755340.
OMA	LYCYQEL.
OrthoDB	EOG447K2W.
Enzyme and pathway databases
BRENDA	2.7.11.24. 984.
Gene expression databases
ArrayExpress	P41808.
Genevestigator	P41808.
GermOnline	YPR054W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08293.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. False negative. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981316.

Entry information

Entry name

SMK1_YEAST

Accession

Primary (citable) accession number: P41808
Secondary accession number(s): D6W460

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program