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Protein

Sporulation-specific mitogen-activated protein kinase SMK1

Gene

SMK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for spore wall assembly. Required for proper deposition of the two outer layers of the spore wall, the chitosan and dityrosine layers. Negatively regulates GSC2, an alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691ATPPROSITE-ProRule annotation
Active sitei166 – 1661Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 529ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: SGD

GO - Biological processi

  1. ascospore wall assembly Source: SGD
  2. MAPK cascade Source: GOC
  3. negative regulation of sporulation resulting in formation of a cellular spore Source: SGD
  4. protein autophosphorylation Source: SGD
  5. protein phosphorylation Source: SGD
  6. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Sporulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34206-MONOMER.
BRENDAi2.7.11.24. 984.
ReactomeiREACT_301206. Signalling to ERK5.
REACT_302117. ERK/MAPK targets.
REACT_311526. ERKs are inactivated.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation-specific mitogen-activated protein kinase SMK1 (EC:2.7.11.24)
Short name:
MAP kinase SMK1
Gene namesi
Name:SMK1
Ordered Locus Names:YPR054W
ORF Names:YP9499.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPR054w.
EuPathDBiFungiDB:YPR054W.
SGDiS000006258. SMK1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Sporulation-specific mitogen-activated protein kinase SMK1PRO_0000186339Add
BLAST

Post-translational modificationi

Dually phosphorylated on Thr-207 and Tyr-209, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41808.

Expressioni

Gene expression databases

GenevestigatoriP41808.

Interactioni

Subunit structurei

Interacts with GSC2.1 Publication

Protein-protein interaction databases

BioGridi36228. 46 interactions.
DIPiDIP-1636N.
IntActiP41808. 5 interactions.
MINTiMINT-400914.
STRINGi4932.YPR054W.

Structurei

3D structure databases

ProteinModelPortaliP41808.
SMRiP41808. Positions 38-382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 337300Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi207 – 2093TXY

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi384 – 3874Poly-Ser

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120669.
HOGENOMiHOG000233024.
InParanoidiP41808.
KOiK08293.
OMAiQFSEFHI.
OrthoDBiEOG78WM27.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCTLTDNTR AINVASNLGA PQQRTIFAKE RISIPGYYEI IQFLGKGAYG
60 70 80 90 100
TVCSVKFKGR SPAARIAVKK ISNIFNKEIL LKRAIRELKF MNFFKGHKNI
110 120 130 140 150
VNLIDLEIVT SSPYDGLYCY QELIDYDLAK VIHSSVQLSE FHIKYFLYQI
160 170 180 190 200
LCGLKYIHSA DVIHRDLKPG NILCTLNGCL KICDFGLARG IHAGFFKCHS
210 220 230 240 250
TVQPHITNYV ATRWYRAPEL LLSNQPYSKS VDIWAVGCIL AEFYARKPVF
260 270 280 290 300
MGRDSMHQIF EIIKVLGTPD KDILIKFGTI KAWNLGKNSN NPVYKKIPWS
310 320 330 340 350
NIFPFASHEA INLIESLLHW DSTHRLNVEQ AISHPFLNEV RKPDDEPVCL
360 370 380
QGPFDFTYES ELNSMSKLRD YLVEEVKNFK TDLSSSSL
Length:388
Mass (Da):44,300
Last modified:November 1, 1995 - v1
Checksum:i081BD00B9DBC3F23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35047 Genomic DNA. Translation: AAB59325.1.
Z49219 Genomic DNA. Translation: CAA89172.1.
Z71255 Genomic DNA. Translation: CAA94999.1.
BK006949 Genomic DNA. Translation: DAA11476.1.
PIRiS48879.
RefSeqiNP_015379.1. NM_001184151.1.

Genome annotation databases

EnsemblFungiiYPR054W; YPR054W; YPR054W.
GeneIDi856167.
KEGGisce:YPR054W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35047 Genomic DNA. Translation: AAB59325.1.
Z49219 Genomic DNA. Translation: CAA89172.1.
Z71255 Genomic DNA. Translation: CAA94999.1.
BK006949 Genomic DNA. Translation: DAA11476.1.
PIRiS48879.
RefSeqiNP_015379.1. NM_001184151.1.

3D structure databases

ProteinModelPortaliP41808.
SMRiP41808. Positions 38-382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36228. 46 interactions.
DIPiDIP-1636N.
IntActiP41808. 5 interactions.
MINTiMINT-400914.
STRINGi4932.YPR054W.

Proteomic databases

PaxDbiP41808.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR054W; YPR054W; YPR054W.
GeneIDi856167.
KEGGisce:YPR054W.

Organism-specific databases

CYGDiYPR054w.
EuPathDBiFungiDB:YPR054W.
SGDiS000006258. SMK1.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120669.
HOGENOMiHOG000233024.
InParanoidiP41808.
KOiK08293.
OMAiQFSEFHI.
OrthoDBiEOG78WM27.

Enzyme and pathway databases

BioCyciYEAST:G3O-34206-MONOMER.
BRENDAi2.7.11.24. 984.
ReactomeiREACT_301206. Signalling to ERK5.
REACT_302117. ERK/MAPK targets.
REACT_311526. ERKs are inactivated.

Miscellaneous databases

NextBioi981316.
PROiP41808.

Gene expression databases

GenevestigatoriP41808.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae."
    Krisak L., Strich R., Winters R.S., Hall J.P., Mallory M.J., Kreitzer D., Tuan R.S., Winter E.
    Genes Dev. 8:2151-2161(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae."
    Huang L.S., Doherty H.K., Herskowitz I.
    Proc. Natl. Acad. Sci. U.S.A. 102:12431-12436(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GSC2.

Entry informationi

Entry nameiSMK1_YEAST
AccessioniPrimary (citable) accession number: P41808
Secondary accession number(s): D6W460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 29, 2015
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.