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P41808

- SMK1_YEAST

UniProt

P41808 - SMK1_YEAST

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Protein
Sporulation-specific mitogen-activated protein kinase SMK1
Gene
SMK1, YPR054W, YP9499.10
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for spore wall assembly. Required for proper deposition of the two outer layers of the spore wall, the chitosan and dityrosine layers. Negatively regulates GSC2, an alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691ATP By similarity
Active sitei166 – 1661Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 529ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: SGD

GO - Biological processi

  1. MAPK cascade Source: GOC
  2. ascospore wall assembly Source: SGD
  3. negative regulation of sporulation resulting in formation of a cellular spore Source: SGD
  4. protein phosphorylation Source: SGD
  5. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Sporulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34206-MONOMER.
BRENDAi2.7.11.24. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation-specific mitogen-activated protein kinase SMK1 (EC:2.7.11.24)
Short name:
MAP kinase SMK1
Gene namesi
Name:SMK1
Ordered Locus Names:YPR054W
ORF Names:YP9499.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR054w.
SGDiS000006258. SMK1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Sporulation-specific mitogen-activated protein kinase SMK1
PRO_0000186339Add
BLAST

Post-translational modificationi

Dually phosphorylated on Thr-207 and Tyr-209, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41808.

Expressioni

Gene expression databases

GenevestigatoriP41808.

Interactioni

Subunit structurei

Interacts with GSC2.1 Publication

Protein-protein interaction databases

BioGridi36228. 46 interactions.
DIPiDIP-1636N.
IntActiP41808. 5 interactions.
MINTiMINT-400914.
STRINGi4932.YPR054W.

Structurei

3D structure databases

ProteinModelPortaliP41808.
SMRiP41808. Positions 23-382.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 337300Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi207 – 2093TXY

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi384 – 3874Poly-Ser

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00730000112267.
HOGENOMiHOG000233024.
KOiK08293.
OMAiIRELKFM.
OrthoDBiEOG78WM27.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41808-1 [UniParc]FASTAAdd to Basket

« Hide

MNCTLTDNTR AINVASNLGA PQQRTIFAKE RISIPGYYEI IQFLGKGAYG    50
TVCSVKFKGR SPAARIAVKK ISNIFNKEIL LKRAIRELKF MNFFKGHKNI 100
VNLIDLEIVT SSPYDGLYCY QELIDYDLAK VIHSSVQLSE FHIKYFLYQI 150
LCGLKYIHSA DVIHRDLKPG NILCTLNGCL KICDFGLARG IHAGFFKCHS 200
TVQPHITNYV ATRWYRAPEL LLSNQPYSKS VDIWAVGCIL AEFYARKPVF 250
MGRDSMHQIF EIIKVLGTPD KDILIKFGTI KAWNLGKNSN NPVYKKIPWS 300
NIFPFASHEA INLIESLLHW DSTHRLNVEQ AISHPFLNEV RKPDDEPVCL 350
QGPFDFTYES ELNSMSKLRD YLVEEVKNFK TDLSSSSL 388
Length:388
Mass (Da):44,300
Last modified:November 1, 1995 - v1
Checksum:i081BD00B9DBC3F23
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L35047 Genomic DNA. Translation: AAB59325.1.
Z49219 Genomic DNA. Translation: CAA89172.1.
Z71255 Genomic DNA. Translation: CAA94999.1.
BK006949 Genomic DNA. Translation: DAA11476.1.
PIRiS48879.
RefSeqiNP_015379.1. NM_001184151.1.

Genome annotation databases

EnsemblFungiiYPR054W; YPR054W; YPR054W.
GeneIDi856167.
KEGGisce:YPR054W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L35047 Genomic DNA. Translation: AAB59325.1 .
Z49219 Genomic DNA. Translation: CAA89172.1 .
Z71255 Genomic DNA. Translation: CAA94999.1 .
BK006949 Genomic DNA. Translation: DAA11476.1 .
PIRi S48879.
RefSeqi NP_015379.1. NM_001184151.1.

3D structure databases

ProteinModelPortali P41808.
SMRi P41808. Positions 23-382.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36228. 46 interactions.
DIPi DIP-1636N.
IntActi P41808. 5 interactions.
MINTi MINT-400914.
STRINGi 4932.YPR054W.

Proteomic databases

PaxDbi P41808.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR054W ; YPR054W ; YPR054W .
GeneIDi 856167.
KEGGi sce:YPR054W.

Organism-specific databases

CYGDi YPR054w.
SGDi S000006258. SMK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00730000112267.
HOGENOMi HOG000233024.
KOi K08293.
OMAi IRELKFM.
OrthoDBi EOG78WM27.

Enzyme and pathway databases

BioCyci YEAST:G3O-34206-MONOMER.
BRENDAi 2.7.11.24. 984.

Miscellaneous databases

NextBioi 981316.

Gene expression databases

Genevestigatori P41808.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae."
    Krisak L., Strich R., Winters R.S., Hall J.P., Mallory M.J., Kreitzer D., Tuan R.S., Winter E.
    Genes Dev. 8:2151-2161(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae."
    Huang L.S., Doherty H.K., Herskowitz I.
    Proc. Natl. Acad. Sci. U.S.A. 102:12431-12436(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GSC2.

Entry informationi

Entry nameiSMK1_YEAST
AccessioniPrimary (citable) accession number: P41808
Secondary accession number(s): D6W460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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