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P41808

- SMK1_YEAST

UniProt

P41808 - SMK1_YEAST

Protein

Sporulation-specific mitogen-activated protein kinase SMK1

Gene

SMK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Required for spore wall assembly. Required for proper deposition of the two outer layers of the spore wall, the chitosan and dityrosine layers. Negatively regulates GSC2, an alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691ATPPROSITE-ProRule annotation
    Active sitei166 – 1661Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi44 – 529ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: SGD

    GO - Biological processi

    1. ascospore wall assembly Source: SGD
    2. MAPK cascade Source: GOC
    3. negative regulation of sporulation resulting in formation of a cellular spore Source: SGD
    4. protein phosphorylation Source: SGD
    5. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradation, Sporulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34206-MONOMER.
    BRENDAi2.7.11.24. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sporulation-specific mitogen-activated protein kinase SMK1 (EC:2.7.11.24)
    Short name:
    MAP kinase SMK1
    Gene namesi
    Name:SMK1
    Ordered Locus Names:YPR054W
    ORF Names:YP9499.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR054w.
    SGDiS000006258. SMK1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 388388Sporulation-specific mitogen-activated protein kinase SMK1PRO_0000186339Add
    BLAST

    Post-translational modificationi

    Dually phosphorylated on Thr-207 and Tyr-209, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP41808.

    Expressioni

    Gene expression databases

    GenevestigatoriP41808.

    Interactioni

    Subunit structurei

    Interacts with GSC2.1 Publication

    Protein-protein interaction databases

    BioGridi36228. 46 interactions.
    DIPiDIP-1636N.
    IntActiP41808. 5 interactions.
    MINTiMINT-400914.
    STRINGi4932.YPR054W.

    Structurei

    3D structure databases

    ProteinModelPortaliP41808.
    SMRiP41808. Positions 23-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 337300Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi207 – 2093TXY

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi384 – 3874Poly-Ser

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000112267.
    HOGENOMiHOG000233024.
    KOiK08293.
    OMAiIRELKFM.
    OrthoDBiEOG78WM27.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41808-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNCTLTDNTR AINVASNLGA PQQRTIFAKE RISIPGYYEI IQFLGKGAYG    50
    TVCSVKFKGR SPAARIAVKK ISNIFNKEIL LKRAIRELKF MNFFKGHKNI 100
    VNLIDLEIVT SSPYDGLYCY QELIDYDLAK VIHSSVQLSE FHIKYFLYQI 150
    LCGLKYIHSA DVIHRDLKPG NILCTLNGCL KICDFGLARG IHAGFFKCHS 200
    TVQPHITNYV ATRWYRAPEL LLSNQPYSKS VDIWAVGCIL AEFYARKPVF 250
    MGRDSMHQIF EIIKVLGTPD KDILIKFGTI KAWNLGKNSN NPVYKKIPWS 300
    NIFPFASHEA INLIESLLHW DSTHRLNVEQ AISHPFLNEV RKPDDEPVCL 350
    QGPFDFTYES ELNSMSKLRD YLVEEVKNFK TDLSSSSL 388
    Length:388
    Mass (Da):44,300
    Last modified:November 1, 1995 - v1
    Checksum:i081BD00B9DBC3F23
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35047 Genomic DNA. Translation: AAB59325.1.
    Z49219 Genomic DNA. Translation: CAA89172.1.
    Z71255 Genomic DNA. Translation: CAA94999.1.
    BK006949 Genomic DNA. Translation: DAA11476.1.
    PIRiS48879.
    RefSeqiNP_015379.1. NM_001184151.1.

    Genome annotation databases

    EnsemblFungiiYPR054W; YPR054W; YPR054W.
    GeneIDi856167.
    KEGGisce:YPR054W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35047 Genomic DNA. Translation: AAB59325.1 .
    Z49219 Genomic DNA. Translation: CAA89172.1 .
    Z71255 Genomic DNA. Translation: CAA94999.1 .
    BK006949 Genomic DNA. Translation: DAA11476.1 .
    PIRi S48879.
    RefSeqi NP_015379.1. NM_001184151.1.

    3D structure databases

    ProteinModelPortali P41808.
    SMRi P41808. Positions 23-382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36228. 46 interactions.
    DIPi DIP-1636N.
    IntActi P41808. 5 interactions.
    MINTi MINT-400914.
    STRINGi 4932.YPR054W.

    Proteomic databases

    PaxDbi P41808.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR054W ; YPR054W ; YPR054W .
    GeneIDi 856167.
    KEGGi sce:YPR054W.

    Organism-specific databases

    CYGDi YPR054w.
    SGDi S000006258. SMK1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000112267.
    HOGENOMi HOG000233024.
    KOi K08293.
    OMAi IRELKFM.
    OrthoDBi EOG78WM27.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34206-MONOMER.
    BRENDAi 2.7.11.24. 984.

    Miscellaneous databases

    NextBioi 981316.

    Gene expression databases

    Genevestigatori P41808.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae."
      Krisak L., Strich R., Winters R.S., Hall J.P., Mallory M.J., Kreitzer D., Tuan R.S., Winter E.
      Genes Dev. 8:2151-2161(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae."
      Huang L.S., Doherty H.K., Herskowitz I.
      Proc. Natl. Acad. Sci. U.S.A. 102:12431-12436(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GSC2.

    Entry informationi

    Entry nameiSMK1_YEAST
    AccessioniPrimary (citable) accession number: P41808
    Secondary accession number(s): D6W460
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3