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P41806 (VMA21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar ATPase assembly integral membrane protein VMA21
Gene names
Name:VMA21
Ordered Locus Names:YGR105W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length77 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions with VOA1 in assembly of the integral membrane sector (also called V0 sector) of the V-ATPase in the endoplasmic reticulum. Escorts the assembled V0 sector in COPII vesicles. Also required for normal packaging of the SNARE BOS1 and possibly the ER to Golgi transport receptor ERV29. Ref.1 Ref.5 Ref.7 Ref.9 Ref.10 Ref.12

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesicleCOPII-coated vesicle membrane; Multi-pass membrane protein Ref.1 Ref.12.

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the VMA21 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7777Vacuolar ATPase assembly integral membrane protein VMA21 HAMAP-Rule MF_03058
PRO_0000065875

Regions

Topological domain1 – 1313Cytoplasmic Potential
Transmembrane14 – 3421Helical; Potential
Topological domain35 – 384Lumenal Potential
Transmembrane39 – 5921Helical; Potential
Topological domain60 – 7718Cytoplasmic Potential
Motif74 – 774Prevents secretion from ER HAMAP-Rule MF_03058

Experimental info

Mutagenesis74 – 752KK → QQ: Mislocalizes to the vacuole membrane and stay associated with the V0 sector after transport out of the ER. Ref.1 Ref.7
Mutagenesis741K → Q: Secretion from ER. Ref.1 Ref.7
Mutagenesis751K → Q: Secretion from ER. Ref.1 Ref.7
Sequence conflict701V → G in AAS56142. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P41806 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D09719B2A2839A4B

FASTA778,435
        10         20         30         40         50         60 
MAVDVPRAVI NKLMLFTAAM VVLPVLTFFI IQQFTPNTLI SGGLAAAMAN VVLIVYIVVA 

        70 
FREDTEDHKV DGNKKED 

« Hide

References

« Hide 'large scale' references
[1]"Vma21p is a yeast membrane protein retained in the endoplasmic reticulum by a di-lysine motif and is required for the assembly of the vacuolar H(+)-ATPase complex."
Hill K.J., Stevens T.H.
Mol. Biol. Cell 5:1039-1050(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 74-LYS-LYS-75.
Strain: SF838-1D.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase."
Ho M.N., Hill K.J., Lindorfer M.A., Stevens T.H.
J. Biol. Chem. 268:221-227(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Role of Vma21p in assembly and transport of the yeast vacuolar ATPase."
Malkus P., Graham L.A., Stevens T.H., Schekman R.
Mol. Biol. Cell 15:5075-5091(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH VMA3; VMA6; VMA11; VMA16 AND VPH1, MUTAGENESIS OF 74-LYS-LYS-75.
[8]"Vma9p (subunit e) is an integral membrane V0 subunit of the yeast V-ATPase."
Compton M.A., Graham L.A., Stevens T.H.
J. Biol. Chem. 281:15312-15319(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VMA3; VMA6; VMA11 AND VPH1.
[9]"PKR1 encodes an assembly factor for the yeast V-type ATPase."
Davis-Kaplan S.R., Compton M.A., Flannery A.R., Ward D.M., Kaplan J., Stevens T.H., Graham L.A.
J. Biol. Chem. 281:32025-32035(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Genetic and molecular interactions of the Erv41p-Erv46p complex involved in transport between the endoplasmic reticulum and Golgi complex."
Welsh L.M., Tong A.H.Y., Boone C., Jensen O.N., Otte S.
J. Cell Sci. 119:4730-4740(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[12]"Voa1p functions in V-ATPase assembly in the yeast endoplasmic reticulum."
Ryan M., Graham L.A., Stevens T.H.
Mol. Biol. Cell 19:5131-5142(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VMA3; VMA6; VMA11; VMA16 AND VOA1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09329 Genomic DNA. Translation: AAA74455.1.
Z72890 Genomic DNA. Translation: CAA97109.1.
AY557816 Genomic DNA. Translation: AAS56142.1.
BK006941 Genomic DNA. Translation: DAA08198.1.
PIRS47941.
RefSeqNP_011619.3. NM_001181234.3.

3D structure databases

ProteinModelPortalP41806.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33348. 580 interactions.
DIPDIP-5526N.
IntActP41806. 10 interactions.
MINTMINT-496678.
STRING4932.YGR105W.

Proteomic databases

MaxQBP41806.
PaxDbP41806.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR105W; YGR105W; YGR105W.
GeneID852997.
KEGGsce:YGR105W.

Organism-specific databases

CYGDYGR105w.
SGDS000003337. VMA21.

Phylogenomic databases

eggNOGNOG140611.
HOGENOMHOG000234560.
OMAVFFTGAM.
OrthoDBEOG7SBP20.

Enzyme and pathway databases

BioCycYEAST:G3O-30815-MONOMER.

Gene expression databases

GenevestigatorP41806.

Family and domain databases

HAMAPMF_03058. VMA21.
InterProIPR019013. VMA21-like_domain.
[Graphical view]
PfamPF09446. VMA21. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972836.

Entry information

Entry nameVMA21_YEAST
AccessionPrimary (citable) accession number: P41806
Secondary accession number(s): D6VUN7, Q6Q5Q3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families