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P41806

- VMA21_YEAST

UniProt

P41806 - VMA21_YEAST

Protein

Vacuolar ATPase assembly integral membrane protein VMA21

Gene

VMA21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Functions with VOA1 in assembly of the integral membrane sector (also called V0 sector) of the V-ATPase in the endoplasmic reticulum. Escorts the assembled V0 sector in COPII vesicles. Also required for normal packaging of the SNARE BOS1 and possibly the ER to Golgi transport receptor ERV29.6 PublicationsUniRule annotation

    GO - Biological processi

    1. vacuolar proton-transporting V-type ATPase complex assembly Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30815-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar ATPase assembly integral membrane protein VMA21UniRule annotation
    Gene namesi
    Name:VMA21UniRule annotation
    Ordered Locus Names:YGR105W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR105w.
    SGDiS000003337. VMA21.

    Subcellular locationi

    Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane UniRule annotation; Multi-pass membrane protein UniRule annotation. Cytoplasmic vesicleCOPII-coated vesicle membrane; Multi-pass membrane protein

    GO - Cellular componenti

    1. endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
    2. endoplasmic reticulum membrane Source: SGD
    3. ER to Golgi transport vesicle membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi74 – 752KK → QQ: Mislocalizes to the vacuole membrane and stay associated with the V0 sector after transport out of the ER.
    Mutagenesisi74 – 741K → Q: Secretion from ER.
    Mutagenesisi75 – 751K → Q: Secretion from ER.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7777Vacuolar ATPase assembly integral membrane protein VMA21PRO_0000065875Add
    BLAST

    Proteomic databases

    MaxQBiP41806.
    PaxDbiP41806.

    Expressioni

    Gene expression databases

    GenevestigatoriP41806.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGP1P253761EBI-20323,EBI-2357
    CHO1P084561EBI-20323,EBI-14055
    EMC2P471331EBI-20323,EBI-25568
    ERG11P106141EBI-20323,EBI-5127
    ERP1Q053591EBI-20323,EBI-6581
    ERV25P548371EBI-20323,EBI-6642
    GAP1P191451EBI-20323,EBI-7314
    OST1P415431EBI-20323,EBI-12651
    PHO84P252971EBI-20323,EBI-13320
    PKR1Q038801EBI-20323,EBI-27252
    PMP2P409751EBI-20323,EBI-2043041
    SAC1P323681EBI-20323,EBI-16210
    SPF1P399861EBI-20323,EBI-3128
    UBA4P388201EBI-20323,EBI-24676
    VMA13P418071EBI-20323,EBI-20281
    VMA6P323661EBI-20323,EBI-20201
    VMA7P391111EBI-20323,EBI-20272
    VMA8P326101EBI-20323,EBI-20264
    VOA1P532621EBI-20323,EBI-2061527
    VPH1P325631EBI-20323,EBI-20455
    YHR146WP388451EBI-20323,EBI-24770

    Protein-protein interaction databases

    BioGridi33348. 583 interactions.
    DIPiDIP-5526N.
    IntActiP41806. 10 interactions.
    MINTiMINT-496678.
    STRINGi4932.YGR105W.

    Structurei

    3D structure databases

    ProteinModelPortaliP41806.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1313CytoplasmicUniRule annotationAdd
    BLAST
    Topological domaini35 – 384LumenalUniRule annotation
    Topological domaini60 – 7718CytoplasmicUniRule annotationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei14 – 3421HelicalUniRule annotationAdd
    BLAST
    Transmembranei39 – 5921HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi74 – 774Prevents secretion from ER

    Sequence similaritiesi

    Belongs to the VMA21 family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG140611.
    HOGENOMiHOG000234560.
    OMAiVFFTGAM.
    OrthoDBiEOG7SBP20.

    Family and domain databases

    HAMAPiMF_03058. VMA21.
    InterProiIPR019013. VMA21-like_domain.
    [Graphical view]
    PfamiPF09446. VMA21. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41806-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVDVPRAVI NKLMLFTAAM VVLPVLTFFI IQQFTPNTLI SGGLAAAMAN   50
    VVLIVYIVVA FREDTEDHKV DGNKKED 77
    Length:77
    Mass (Da):8,435
    Last modified:November 1, 1995 - v1
    Checksum:iD09719B2A2839A4B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701V → G in AAS56142. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09329 Genomic DNA. Translation: AAA74455.1.
    Z72890 Genomic DNA. Translation: CAA97109.1.
    AY557816 Genomic DNA. Translation: AAS56142.1.
    BK006941 Genomic DNA. Translation: DAA08198.1.
    PIRiS47941.
    RefSeqiNP_011619.3. NM_001181234.3.

    Genome annotation databases

    EnsemblFungiiYGR105W; YGR105W; YGR105W.
    GeneIDi852997.
    KEGGisce:YGR105W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09329 Genomic DNA. Translation: AAA74455.1 .
    Z72890 Genomic DNA. Translation: CAA97109.1 .
    AY557816 Genomic DNA. Translation: AAS56142.1 .
    BK006941 Genomic DNA. Translation: DAA08198.1 .
    PIRi S47941.
    RefSeqi NP_011619.3. NM_001181234.3.

    3D structure databases

    ProteinModelPortali P41806.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33348. 583 interactions.
    DIPi DIP-5526N.
    IntActi P41806. 10 interactions.
    MINTi MINT-496678.
    STRINGi 4932.YGR105W.

    Proteomic databases

    MaxQBi P41806.
    PaxDbi P41806.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR105W ; YGR105W ; YGR105W .
    GeneIDi 852997.
    KEGGi sce:YGR105W.

    Organism-specific databases

    CYGDi YGR105w.
    SGDi S000003337. VMA21.

    Phylogenomic databases

    eggNOGi NOG140611.
    HOGENOMi HOG000234560.
    OMAi VFFTGAM.
    OrthoDBi EOG7SBP20.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30815-MONOMER.

    Miscellaneous databases

    NextBioi 972836.

    Gene expression databases

    Genevestigatori P41806.

    Family and domain databases

    HAMAPi MF_03058. VMA21.
    InterProi IPR019013. VMA21-like_domain.
    [Graphical view ]
    Pfami PF09446. VMA21. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vma21p is a yeast membrane protein retained in the endoplasmic reticulum by a di-lysine motif and is required for the assembly of the vacuolar H(+)-ATPase complex."
      Hill K.J., Stevens T.H.
      Mol. Biol. Cell 5:1039-1050(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 74-LYS-LYS-75.
      Strain: SF838-1D.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase."
      Ho M.N., Hill K.J., Lindorfer M.A., Stevens T.H.
      J. Biol. Chem. 268:221-227(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Role of Vma21p in assembly and transport of the yeast vacuolar ATPase."
      Malkus P., Graham L.A., Stevens T.H., Schekman R.
      Mol. Biol. Cell 15:5075-5091(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VMA3; VMA6; VMA11; VMA16 AND VPH1, MUTAGENESIS OF 74-LYS-LYS-75.
    8. "Vma9p (subunit e) is an integral membrane V0 subunit of the yeast V-ATPase."
      Compton M.A., Graham L.A., Stevens T.H.
      J. Biol. Chem. 281:15312-15319(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VMA3; VMA6; VMA11 AND VPH1.
    9. Cited for: FUNCTION.
    10. "Genetic and molecular interactions of the Erv41p-Erv46p complex involved in transport between the endoplasmic reticulum and Golgi complex."
      Welsh L.M., Tong A.H.Y., Boone C., Jensen O.N., Otte S.
      J. Cell Sci. 119:4730-4740(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    12. "Voa1p functions in V-ATPase assembly in the yeast endoplasmic reticulum."
      Ryan M., Graham L.A., Stevens T.H.
      Mol. Biol. Cell 19:5131-5142(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VMA3; VMA6; VMA11; VMA16 AND VOA1.

    Entry informationi

    Entry nameiVMA21_YEAST
    AccessioniPrimary (citable) accession number: P41806
    Secondary accession number(s): D6VUN7, Q6Q5Q3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1480 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3