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P41806

- VMA21_YEAST

UniProt

P41806 - VMA21_YEAST

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Protein
Vacuolar ATPase assembly integral membrane protein VMA21
Gene
VMA21, YGR105W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions with VOA1 in assembly of the integral membrane sector (also called V0 sector) of the V-ATPase in the endoplasmic reticulum. Escorts the assembled V0 sector in COPII vesicles. Also required for normal packaging of the SNARE BOS1 and possibly the ER to Golgi transport receptor ERV29.6 Publications

GO - Biological processi

  1. vacuolar proton-transporting V-type ATPase complex assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30815-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar ATPase assembly integral membrane protein VMA21
Gene namesi
Name:VMA21
Ordered Locus Names:YGR105W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR105w.
SGDiS000003337. VMA21.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesicleCOPII-coated vesicle membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei14 – 3421Helical; Reviewed prediction
Add
BLAST
Topological domaini35 – 384Lumenal Reviewed prediction
Transmembranei39 – 5921Helical; Reviewed prediction
Add
BLAST
Topological domaini60 – 7718Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. ER to Golgi transport vesicle membrane Source: UniProtKB-SubCell
  2. endoplasmic reticulum membrane Source: SGD
  3. endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi74 – 752KK → QQ: Mislocalizes to the vacuole membrane and stay associated with the V0 sector after transport out of the ER. 2 Publications
Mutagenesisi74 – 741K → Q: Secretion from ER. 2 Publications
Mutagenesisi75 – 751K → Q: Secretion from ER. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7777Vacuolar ATPase assembly integral membrane protein VMA21UniRule annotation
PRO_0000065875Add
BLAST

Proteomic databases

MaxQBiP41806.
PaxDbiP41806.

Expressioni

Gene expression databases

GenevestigatoriP41806.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP1P253761EBI-20323,EBI-2357
CHO1P084561EBI-20323,EBI-14055
EMC2P471331EBI-20323,EBI-25568
ERG11P106141EBI-20323,EBI-5127
ERP1Q053591EBI-20323,EBI-6581
ERV25P548371EBI-20323,EBI-6642
GAP1P191451EBI-20323,EBI-7314
OST1P415431EBI-20323,EBI-12651
PHO84P252971EBI-20323,EBI-13320
PKR1Q038801EBI-20323,EBI-27252
PMP2P409751EBI-20323,EBI-2043041
SAC1P323681EBI-20323,EBI-16210
SPF1P399861EBI-20323,EBI-3128
UBA4P388201EBI-20323,EBI-24676
VMA13P418071EBI-20323,EBI-20281
VMA6P323661EBI-20323,EBI-20201
VMA7P391111EBI-20323,EBI-20272
VMA8P326101EBI-20323,EBI-20264
VOA1P532621EBI-20323,EBI-2061527
VPH1P325631EBI-20323,EBI-20455
YHR146WP388451EBI-20323,EBI-24770

Protein-protein interaction databases

BioGridi33348. 583 interactions.
DIPiDIP-5526N.
IntActiP41806. 10 interactions.
MINTiMINT-496678.
STRINGi4932.YGR105W.

Structurei

3D structure databases

ProteinModelPortaliP41806.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi74 – 774Prevents secretion from ERUniRule annotation

Sequence similaritiesi

Belongs to the VMA21 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG140611.
HOGENOMiHOG000234560.
OMAiVFFTGAM.
OrthoDBiEOG7SBP20.

Family and domain databases

HAMAPiMF_03058. VMA21.
InterProiIPR019013. VMA21-like_domain.
[Graphical view]
PfamiPF09446. VMA21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41806-1 [UniParc]FASTAAdd to Basket

« Hide

MAVDVPRAVI NKLMLFTAAM VVLPVLTFFI IQQFTPNTLI SGGLAAAMAN   50
VVLIVYIVVA FREDTEDHKV DGNKKED 77
Length:77
Mass (Da):8,435
Last modified:November 1, 1995 - v1
Checksum:iD09719B2A2839A4B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701V → G in AAS56142. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09329 Genomic DNA. Translation: AAA74455.1.
Z72890 Genomic DNA. Translation: CAA97109.1.
AY557816 Genomic DNA. Translation: AAS56142.1.
BK006941 Genomic DNA. Translation: DAA08198.1.
PIRiS47941.
RefSeqiNP_011619.3. NM_001181234.3.

Genome annotation databases

EnsemblFungiiYGR105W; YGR105W; YGR105W.
GeneIDi852997.
KEGGisce:YGR105W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09329 Genomic DNA. Translation: AAA74455.1 .
Z72890 Genomic DNA. Translation: CAA97109.1 .
AY557816 Genomic DNA. Translation: AAS56142.1 .
BK006941 Genomic DNA. Translation: DAA08198.1 .
PIRi S47941.
RefSeqi NP_011619.3. NM_001181234.3.

3D structure databases

ProteinModelPortali P41806.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33348. 583 interactions.
DIPi DIP-5526N.
IntActi P41806. 10 interactions.
MINTi MINT-496678.
STRINGi 4932.YGR105W.

Proteomic databases

MaxQBi P41806.
PaxDbi P41806.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR105W ; YGR105W ; YGR105W .
GeneIDi 852997.
KEGGi sce:YGR105W.

Organism-specific databases

CYGDi YGR105w.
SGDi S000003337. VMA21.

Phylogenomic databases

eggNOGi NOG140611.
HOGENOMi HOG000234560.
OMAi VFFTGAM.
OrthoDBi EOG7SBP20.

Enzyme and pathway databases

BioCyci YEAST:G3O-30815-MONOMER.

Miscellaneous databases

NextBioi 972836.

Gene expression databases

Genevestigatori P41806.

Family and domain databases

HAMAPi MF_03058. VMA21.
InterProi IPR019013. VMA21-like_domain.
[Graphical view ]
Pfami PF09446. VMA21. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Vma21p is a yeast membrane protein retained in the endoplasmic reticulum by a di-lysine motif and is required for the assembly of the vacuolar H(+)-ATPase complex."
    Hill K.J., Stevens T.H.
    Mol. Biol. Cell 5:1039-1050(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 74-LYS-LYS-75.
    Strain: SF838-1D.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase."
    Ho M.N., Hill K.J., Lindorfer M.A., Stevens T.H.
    J. Biol. Chem. 268:221-227(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Role of Vma21p in assembly and transport of the yeast vacuolar ATPase."
    Malkus P., Graham L.A., Stevens T.H., Schekman R.
    Mol. Biol. Cell 15:5075-5091(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VMA3; VMA6; VMA11; VMA16 AND VPH1, MUTAGENESIS OF 74-LYS-LYS-75.
  8. "Vma9p (subunit e) is an integral membrane V0 subunit of the yeast V-ATPase."
    Compton M.A., Graham L.A., Stevens T.H.
    J. Biol. Chem. 281:15312-15319(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VMA3; VMA6; VMA11 AND VPH1.
  9. Cited for: FUNCTION.
  10. "Genetic and molecular interactions of the Erv41p-Erv46p complex involved in transport between the endoplasmic reticulum and Golgi complex."
    Welsh L.M., Tong A.H.Y., Boone C., Jensen O.N., Otte S.
    J. Cell Sci. 119:4730-4740(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  12. "Voa1p functions in V-ATPase assembly in the yeast endoplasmic reticulum."
    Ryan M., Graham L.A., Stevens T.H.
    Mol. Biol. Cell 19:5131-5142(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VMA3; VMA6; VMA11; VMA16 AND VOA1.

Entry informationi

Entry nameiVMA21_YEAST
AccessioniPrimary (citable) accession number: P41806
Secondary accession number(s): D6VUN7, Q6Q5Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi