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Protein

60S ribosomal protein L10

Gene

RPL10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • ribosomal large subunit assembly Source: SGD
  • translational termination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32227-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L10
Alternative name(s):
L9
Ubiquinol-cytochrome C reductase complex subunit VI-requiring protein
Gene namesi
Name:RPL10
Synonyms:GRC5, QSR1
Ordered Locus Names:YLR075W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR075W.
SGDiS000004065. RPL10.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi194 – 1941G → D in QSR1-1; synthetic lethal. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 22122060S ribosomal protein L10PRO_0000147129Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei143 – 1431PhosphoserineCombined sources
Modified residuei205 – 2051PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41805.
PeptideAtlasiP41805.
PRIDEiP41805.

PTM databases

iPTMnetiP41805.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31349. 91 interactions.
DIPiDIP-2507N.
IntActiP41805. 89 interactions.
MINTiMINT-1326100.

Structurei

Secondary structure

1
221
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 116Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-I4-168[»]
3J6Xelectron microscopy6.10501-221[»]
3J6Yelectron microscopy6.10501-221[»]
3J77electron microscopy6.20601-221[»]
3J78electron microscopy6.30601-221[»]
4U3MX-ray3.00M0/m02-221[»]
4U3NX-ray3.20M0/m02-221[»]
4U3UX-ray2.90M0/m02-221[»]
4U4NX-ray3.10M0/m02-221[»]
4U4OX-ray3.60M0/m02-221[»]
4U4QX-ray3.00M0/m02-221[»]
4U4RX-ray2.80M0/m02-221[»]
4U4UX-ray3.00M0/m02-221[»]
4U4YX-ray3.20M0/m02-221[»]
4U4ZX-ray3.10M0/m02-221[»]
4U50X-ray3.20M0/m02-221[»]
4U51X-ray3.20M0/m02-221[»]
4U52X-ray3.00M0/m02-221[»]
4U53X-ray3.30M0/m02-221[»]
4U55X-ray3.20M0/m02-221[»]
4U56X-ray3.45M0/m02-221[»]
4U6FX-ray3.10M0/m02-221[»]
4V4Belectron microscopy11.70BI1-168[»]
4V6Ielectron microscopy8.80BI1-221[»]
4V7RX-ray4.00BJ/DJ1-221[»]
4V88X-ray3.00BI/DI1-221[»]
4V8Telectron microscopy8.10I1-221[»]
4V8Yelectron microscopy4.30BI2-221[»]
4V8Zelectron microscopy6.60BI2-221[»]
4V91electron microscopy3.70I1-221[»]
4ZOXX-ray1.60B1-20[»]
ProteinModelPortaliP41805.
SMRiP41805. Positions 3-221.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41805.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati167 – 181151Add
BLAST
Repeati200 – 216172Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni167 – 216502 X 15-17 AA approximate repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein L10e family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000003897.
HOGENOMiHOG000223754.
InParanoidiP41805.
KOiK02866.
OMAiEHKMATG.
OrthoDBiEOG71P2N4.

Family and domain databases

Gene3Di3.90.1170.10. 1 hit.
InterProiIPR001197. Ribosomal_L10e.
IPR016180. Ribosomal_L10e/L16.
IPR018255. Ribosomal_L10e_CS.
[Graphical view]
PfamiPF00252. Ribosomal_L16. 1 hit.
[Graphical view]
PIRSFiPIRSF005590. Ribosomal_L10. 1 hit.
SUPFAMiSSF54686. SSF54686. 1 hit.
TIGRFAMsiTIGR00279. uL16_euk_arch. 1 hit.
PROSITEiPS01257. RIBOSOMAL_L10E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41805-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV
60 70 80 90 100
HLVSNELEQL SSEALEAARI CANKYMTTVS GRDAFHLRVR VHPFHVLRIN
110 120 130 140 150
KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE
160 170 180 190 200
GLRRARYKFP GQQKIILSKK WGFTNLDRPE YLKKREAGEV KDDGAFVKFL
210 220
SKKGSLENNI REFPEYFAAQ A
Length:221
Mass (Da):25,361
Last modified:November 1, 1995 - v1
Checksum:i6FF976A3800F347D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06952 Genomic DNA. Translation: AAA81534.1.
X78887 Genomic DNA. Translation: CAA55485.1.
Z73247 Genomic DNA. Translation: CAA97632.1.
AY693034 Genomic DNA. Translation: AAT93053.1.
BK006945 Genomic DNA. Translation: DAA09392.1.
PIRiA57296.
RefSeqiNP_013176.1. NM_001181962.1.

Genome annotation databases

EnsemblFungiiYLR075W; YLR075W; YLR075W.
GeneIDi850764.
KEGGisce:YLR075W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06952 Genomic DNA. Translation: AAA81534.1.
X78887 Genomic DNA. Translation: CAA55485.1.
Z73247 Genomic DNA. Translation: CAA97632.1.
AY693034 Genomic DNA. Translation: AAT93053.1.
BK006945 Genomic DNA. Translation: DAA09392.1.
PIRiA57296.
RefSeqiNP_013176.1. NM_001181962.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-I4-168[»]
3J6Xelectron microscopy6.10501-221[»]
3J6Yelectron microscopy6.10501-221[»]
3J77electron microscopy6.20601-221[»]
3J78electron microscopy6.30601-221[»]
4U3MX-ray3.00M0/m02-221[»]
4U3NX-ray3.20M0/m02-221[»]
4U3UX-ray2.90M0/m02-221[»]
4U4NX-ray3.10M0/m02-221[»]
4U4OX-ray3.60M0/m02-221[»]
4U4QX-ray3.00M0/m02-221[»]
4U4RX-ray2.80M0/m02-221[»]
4U4UX-ray3.00M0/m02-221[»]
4U4YX-ray3.20M0/m02-221[»]
4U4ZX-ray3.10M0/m02-221[»]
4U50X-ray3.20M0/m02-221[»]
4U51X-ray3.20M0/m02-221[»]
4U52X-ray3.00M0/m02-221[»]
4U53X-ray3.30M0/m02-221[»]
4U55X-ray3.20M0/m02-221[»]
4U56X-ray3.45M0/m02-221[»]
4U6FX-ray3.10M0/m02-221[»]
4V4Belectron microscopy11.70BI1-168[»]
4V6Ielectron microscopy8.80BI1-221[»]
4V7RX-ray4.00BJ/DJ1-221[»]
4V88X-ray3.00BI/DI1-221[»]
4V8Telectron microscopy8.10I1-221[»]
4V8Yelectron microscopy4.30BI2-221[»]
4V8Zelectron microscopy6.60BI2-221[»]
4V91electron microscopy3.70I1-221[»]
4ZOXX-ray1.60B1-20[»]
ProteinModelPortaliP41805.
SMRiP41805. Positions 3-221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31349. 91 interactions.
DIPiDIP-2507N.
IntActiP41805. 89 interactions.
MINTiMINT-1326100.

PTM databases

iPTMnetiP41805.

Proteomic databases

MaxQBiP41805.
PeptideAtlasiP41805.
PRIDEiP41805.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR075W; YLR075W; YLR075W.
GeneIDi850764.
KEGGisce:YLR075W.

Organism-specific databases

EuPathDBiFungiDB:YLR075W.
SGDiS000004065. RPL10.

Phylogenomic databases

GeneTreeiENSGT00390000003897.
HOGENOMiHOG000223754.
InParanoidiP41805.
KOiK02866.
OMAiEHKMATG.
OrthoDBiEOG71P2N4.

Enzyme and pathway databases

BioCyciYEAST:G3O-32227-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP41805.
NextBioi966921.
PROiP41805.

Family and domain databases

Gene3Di3.90.1170.10. 1 hit.
InterProiIPR001197. Ribosomal_L10e.
IPR016180. Ribosomal_L10e/L16.
IPR018255. Ribosomal_L10e_CS.
[Graphical view]
PfamiPF00252. Ribosomal_L16. 1 hit.
[Graphical view]
PIRSFiPIRSF005590. Ribosomal_L10. 1 hit.
SUPFAMiSSF54686. SSF54686. 1 hit.
TIGRFAMsiTIGR00279. uL16_euk_arch. 1 hit.
PROSITEiPS01257. RIBOSOMAL_L10E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "QSR1, an essential yeast gene with a genetic relationship to a subunit of the mitochondrial cytochrome bc1 complex, is homologous to a gene implicated in eukaryotic cell differentiation."
    Tron T., Yang M., Dick F.A., Schmitt M.E., Trumpower B.L.
    J. Biol. Chem. 270:9961-9970(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-194.
  2. "The yeast growth control gene GRC5 is highly homologous to the mammalian putative tumor suppressor gene QM."
    Koller H.T., Klade T., Ellinger A., Breitenbach M.
    Yeast 12:53-65(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Ribosomal protein L9 is the product of GRC5, a homolog of the putative tumor suppressor QM in S. cerevisiae."
    Nika J., Erickson F.L., Hannig E.M.
    Yeast 13:1155-1166(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS A RIBOSOMAL PROTEIN.
  7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  8. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143 AND SER-205, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-205, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 4-168, ELECTRON MICROSCOPY.
  14. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 2-169, ELECTRON MICROSCOPY.
  15. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  16. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL10_YEAST
AccessioniPrimary (citable) accession number: P41805
Secondary accession number(s): D6VY76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 20, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.