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Protein

Nucleolar and coiled-body phosphoprotein 1

Gene

Nolc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • box C/D snoRNA binding Source: RGD
  • box H/ACA snoRNA binding Source: RGD
  • GTP binding Source: UniProtKB-KW
  • nuclear localization sequence binding Source: RGD
  • protein domain specific binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: RGD
  • transcription factor binding Source: RGD

GO - Biological processi

  • box H/ACA snoRNA metabolic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of transcription, DNA-templated Source: RGD
  • regulation of protein import into nucleus Source: RGD
  • response to osmotic stress Source: RGD
Complete GO annotation...

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar and coiled-body phosphoprotein 1
Alternative name(s):
140 kDa nucleolar phosphoprotein
Short name:
Nopp140
Nucleolar 130 kDa protein
Nucleolar phosphoprotein p130
Gene namesi
Name:Nolc1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621578. Nolc1.

Subcellular locationi

  • Cytoplasm
  • Nucleusnucleolus

  • Note: Shuttles on curvilinear tracks between nucleolus and cytoplasm. These tracks extend from the dense fibrillar component of the nucleolus across the nucleoplasm to a limited number of nuclear pore complexes.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleolus Source: RGD
  • nucleoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 704704Nucleolar and coiled-body phosphoprotein 1PRO_0000096943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331N6-acetyllysineBy similarity
Cross-linki67 – 67Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei87 – 871PhosphoserineBy similarity
Modified residuei91 – 911PhosphoserineBy similarity
Cross-linki196 – 196Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei372 – 3721PhosphoserineBy similarity
Modified residuei373 – 3731PhosphoserineBy similarity
Modified residuei376 – 3761PhosphoserineBy similarity
Modified residuei424 – 4241N6-acetyllysine; alternateBy similarity
Cross-linki424 – 424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei465 – 4651PhosphoserineBy similarity
Modified residuei567 – 5671PhosphoserineCombined sources
Modified residuei587 – 5871PhosphoserineBy similarity
Cross-linki609 – 609Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei612 – 6121PhosphothreonineBy similarity
Modified residuei615 – 6151PhosphothreonineBy similarity
Modified residuei627 – 6271PhosphoserineBy similarity
Modified residuei648 – 6481PhosphoserineBy similarity
Modified residuei668 – 6681N6-acetyllysineBy similarity
Modified residuei691 – 6911PhosphoserineBy similarity
Modified residuei703 – 7031PhosphoserineCombined sources

Post-translational modificationi

Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. NOPP140 is one of the mostly phosphorylated proteins in the cell.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP41777.
PRIDEiP41777.

PTM databases

iPTMnetiP41777.
PhosphoSiteiP41777.

Interactioni

Subunit structurei

Interacts with DKC1/NAP57, NOP58 and fibrillarin.1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: RGD
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi249215. 2 interactions.
STRINGi10116.ENSRNOP00000025788.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 4233LisHPROSITE-ProRule annotationAdd
BLAST
Repeati84 – 9512Acidic serine cluster 1Add
BLAST
Repeati127 – 13812Acidic serine cluster 2Add
BLAST
Repeati170 – 18112Acidic serine cluster 3Add
BLAST
Repeati231 – 24212Acidic serine cluster 4Add
BLAST
Repeati274 – 28512Acidic serine cluster 5Add
BLAST
Repeati335 – 34612Acidic serine cluster 6Add
BLAST
Repeati373 – 38412Acidic serine cluster 7Add
BLAST
Repeati434 – 44512Acidic serine cluster 8Add
BLAST
Repeati479 – 49012Acidic serine cluster 9Add
BLAST
Repeati524 – 53512Acidic serine cluster 10Add
BLAST
Repeati559 – 57012Acidic serine cluster 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni84 – 57048711 X 12 AA approximate repeats of an acidic serine clusterAdd
BLAST

Sequence similaritiesi

Contains 1 LisH domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2992. Eukaryota.
ENOG4111U5P. LUCA.
HOGENOMiHOG000113823.
HOVERGENiHBG075370.
InParanoidiP41777.
PhylomeDBiP41777.

Family and domain databases

InterProiIPR006594. LisH.
IPR007718. SRP40_C.
[Graphical view]
PfamiPF05022. SRP40_C. 1 hit.
[Graphical view]
PROSITEiPS50896. LISH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTGLRRVV PSDLYPLVLG FLRDNQLSEV ASKFAKATGA TQQDANASSL
60 70 80 90 100
LDIYSFWLKS TKAPKVKLQS NGPVAKKAKK ETSSSDSSED SSEEEDKAQV
110 120 130 140 150
PTQKAAAPAK RASLPQHAGK AAAKASESSS SEESSEEEEE KDKKKKPVQQ
160 170 180 190 200
KAVKPQAKAV RPPPKKAESS ESESDSSSED EAPQTQKPKA AATAAKAPTK
210 220 230 240 250
AQTKAPAKPG PPAKAQPKAA NGKAGSSSSS SSSSSSDDSE EEKKAAAPLK
260 270 280 290 300
KTAPKKQVVA KAPVKVTAAP TQKSSSSEDS SSEEEEEQKK PMKKKAGPYS
310 320 330 340 350
SVPPPSVSLS KKSVGAQSPK KAAAQTQPAD SSADSSEESD SSSEEEKKTP
360 370 380 390 400
AKTVVSKTPA KPAPVKKKAE SSSDSSDSDS SEDEAPAKPV SATKSPLSKP
410 420 430 440 450
AVTPKPPAAK AVATPKQPAG SGQKPQSRKA DSSSSEEESS SSEEEATKKS
460 470 480 490 500
VTTPKARVTA KAAPSLPAKQ APRAGGDSSS DSESSSSEEE KKTPPKPPAK
510 520 530 540 550
KKAAGAAVPK PTPVKKAAAE SSSSSSSSED SSEEEKKKPK SKATPKPQAG
560 570 580 590 600
KANGVPASQN GKAGKESEEE EEDTEQNKKA AGTKPGSGKK RKHNETADEA
610 620 630 640 650
ATPQSKKVKL QTPNTFPKRK KGEKRASSPF RRVREEEIEV DSRVADNSFD
660 670 680 690 700
AKRGAAGDWG ERANQVLKFT KGKSFRHEKT KKKRGSYRGG SISVQVNSVK

FDSE
Length:704
Mass (Da):73,563
Last modified:November 1, 1995 - v1
Checksum:i14DF1BF2DE483EA3
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti150 – 1501Missing in Nopp140b.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94287 mRNA. Translation: AAA41718.1.
M94288 mRNA. Translation: AAA41719.1.
PIRiB42680.
RefSeqiNP_074060.1. NM_022869.1.
UniGeneiRn.9517.

Genome annotation databases

GeneIDi64896.
KEGGirno:64896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94287 mRNA. Translation: AAA41718.1.
M94288 mRNA. Translation: AAA41719.1.
PIRiB42680.
RefSeqiNP_074060.1. NM_022869.1.
UniGeneiRn.9517.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249215. 2 interactions.
STRINGi10116.ENSRNOP00000025788.

PTM databases

iPTMnetiP41777.
PhosphoSiteiP41777.

Proteomic databases

PaxDbiP41777.
PRIDEiP41777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64896.
KEGGirno:64896.

Organism-specific databases

CTDi9221.
RGDi621578. Nolc1.

Phylogenomic databases

eggNOGiKOG2992. Eukaryota.
ENOG4111U5P. LUCA.
HOGENOMiHOG000113823.
HOVERGENiHBG075370.
InParanoidiP41777.
PhylomeDBiP41777.

Miscellaneous databases

PROiP41777.

Family and domain databases

InterProiIPR006594. LisH.
IPR007718. SRP40_C.
[Graphical view]
PfamiPF05022. SRP40_C. 1 hit.
[Graphical view]
PROSITEiPS50896. LISH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOLC1_RAT
AccessioniPrimary (citable) accession number: P41777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.