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Protein

Nucleolar and coiled-body phosphoprotein 1

Gene

Nolc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities.By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • box C/D snoRNA binding Source: RGD
  • box H/ACA snoRNA binding Source: RGD
  • GTP binding Source: UniProtKB-KW
  • nuclear localization sequence binding Source: RGD
  • protein domain specific binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: RGD
  • transcription factor binding Source: RGD

GO - Biological processi

  • box H/ACA snoRNA metabolic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of transcription, DNA-templated Source: RGD
  • regulation of protein import into nucleus Source: RGD
  • response to osmotic stress Source: RGD

Keywordsi

LigandATP-binding, GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar and coiled-body phosphoprotein 1Curated
Alternative name(s):
140 kDa nucleolar phosphoprotein1 Publication
Short name:
Nopp1401 Publication
Nucleolar 130 kDa protein
Nucleolar phosphoprotein p130
Gene namesi
Name:Nolc1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componentsi: Chromosome 1, Unplaced

Organism-specific databases

RGDi621578. Nolc1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleusnucleolus 1 Publication

  • Note: Shuttles on curvilinear tracks between nucleolus and cytoplasm. These tracks extend from the dense fibrillar component of the nucleolus across the nucleoplasm to a limited number of nuclear pore complexes.1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: RGD

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000969431 – 704Nucleolar and coiled-body phosphoprotein 1Add BLAST704

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-acetyllysineBy similarity1
Cross-linki67Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei87PhosphoserineBy similarity1
Modified residuei91PhosphoserineBy similarity1
Cross-linki196Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei372PhosphoserineBy similarity1
Modified residuei373PhosphoserineBy similarity1
Modified residuei376PhosphoserineBy similarity1
Modified residuei424N6-acetyllysine; alternateBy similarity1
Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei465PhosphoserineBy similarity1
Modified residuei567PhosphoserineCombined sources1
Modified residuei587PhosphoserineBy similarity1
Cross-linki609Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei612PhosphothreonineBy similarity1
Modified residuei615PhosphothreonineBy similarity1
Modified residuei627PhosphoserineBy similarity1
Modified residuei648PhosphoserineBy similarity1
Modified residuei668N6-acetyllysineBy similarity1
Modified residuei688Omega-N-methylarginineBy similarity1
Modified residuei691PhosphoserineBy similarity1
Modified residuei703PhosphoserineCombined sources1

Post-translational modificationi

Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. NOLC1 is one of the mostly phosphorylated proteins in the cell.1 Publication
Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP41777.
PRIDEiP41777.

PTM databases

iPTMnetiP41777.
PhosphoSitePlusiP41777.

Interactioni

Subunit structurei

Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II (By similarity). Interacts with DKC1/NAP57, NOP58 and fibrillarin (PubMed:10679015).By similarity1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: RGD
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi249215. 2 interactors.
STRINGi10116.ENSRNOP00000025788.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 42LisHPROSITE-ProRule annotationAdd BLAST33
Repeati84 – 95Acidic serine cluster 1Add BLAST12
Repeati127 – 138Acidic serine cluster 2Add BLAST12
Repeati170 – 181Acidic serine cluster 3Add BLAST12
Repeati231 – 242Acidic serine cluster 4Add BLAST12
Repeati274 – 285Acidic serine cluster 5Add BLAST12
Repeati335 – 346Acidic serine cluster 6Add BLAST12
Repeati373 – 384Acidic serine cluster 7Add BLAST12
Repeati434 – 445Acidic serine cluster 8Add BLAST12
Repeati479 – 490Acidic serine cluster 9Add BLAST12
Repeati524 – 535Acidic serine cluster 10Add BLAST12
Repeati559 – 570Acidic serine cluster 11Add BLAST12

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 57011 X 12 AA approximate repeats of an acidic serine clusterAdd BLAST487

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2992. Eukaryota.
ENOG4111U5P. LUCA.
HOGENOMiHOG000113823.
HOVERGENiHBG075370.
InParanoidiP41777.
PhylomeDBiP41777.

Family and domain databases

InterProiView protein in InterPro
IPR006594. LisH.
IPR007718. SRP40_C.
PfamiView protein in Pfam
PF05022. SRP40_C. 1 hit.
PROSITEiView protein in PROSITE
PS50896. LISH. 1 hit.

Sequencei

Sequence statusi: Complete.

P41777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTGLRRVV PSDLYPLVLG FLRDNQLSEV ASKFAKATGA TQQDANASSL
60 70 80 90 100
LDIYSFWLKS TKAPKVKLQS NGPVAKKAKK ETSSSDSSED SSEEEDKAQV
110 120 130 140 150
PTQKAAAPAK RASLPQHAGK AAAKASESSS SEESSEEEEE EDKKKKPVQQ
160 170 180 190 200
KAVKPQAKAV RPPPKKAESS ESESDSSSED EAPQTQKPKA AATAAKAPTK
210 220 230 240 250
AQTKAPAKPG PPAKAQPKAA NGKAGSSSSS SSSSSSDDSE EEKKAAAPLK
260 270 280 290 300
KTAPKKQVVA KAPVKVTAAP TQKSSSSEDS SSEEEEEQKK PMKKKAGPYS
310 320 330 340 350
SVPPPSVSLS KKSVGAQSPK KAAAQTQPAD SSADSSEESD SSSEEEKKTP
360 370 380 390 400
AKTVVSKTPA KPAPVKKKAE SSSDSSDSDS SEDEAPAKPV SATKSPLSKP
410 420 430 440 450
AVTPKPPAAK AVATPKQPAG SGQKPQSRKA DSSSSEEESS SSEEEATKKS
460 470 480 490 500
VTTPKARVTA KAAPSLPAKQ APRAGGDSSS DSESSSSEEE KKTPPKPPAK
510 520 530 540 550
KKAAGAAVPK PTPVKKAAAE SSSSSSSSED SSEEEKKKPK SKATPKPQAG
560 570 580 590 600
KANGVPASQN GKAGKESEEE EEDTEQNKKA AGTKPGSGKK RKHNETADEA
610 620 630 640 650
ATPQSKKVKL QTPNTFPKRK KGEKRASSPF RRVREEEIEV DSRVADNSFD
660 670 680 690 700
AKRGAAGDWG ERANQVLKFT KGKSFRHEKT KKKRGSYRGG SISVQVNSVK

FDSE
Length:704
Mass (Da):73,564
Last modified:April 12, 2017 - v2
Checksum:i0A911BF1C15E9DBC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141E → K in AAA41718 (PubMed:1623516).1
Sequence conflicti141E → K in AAA41719 (PubMed:1623516).1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti150Missing in Nopp140b. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94287 mRNA. Translation: AAA41718.1.
M94288 mRNA. Translation: AAA41719.1.
AC119478 Genomic DNA. No translation available.
PIRiB42680.
RefSeqiNP_074060.1. NM_022869.1.
UniGeneiRn.9517.

Genome annotation databases

GeneIDi64896.
KEGGirno:64896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94287 mRNA. Translation: AAA41718.1.
M94288 mRNA. Translation: AAA41719.1.
AC119478 Genomic DNA. No translation available.
PIRiB42680.
RefSeqiNP_074060.1. NM_022869.1.
UniGeneiRn.9517.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249215. 2 interactors.
STRINGi10116.ENSRNOP00000025788.

PTM databases

iPTMnetiP41777.
PhosphoSitePlusiP41777.

Proteomic databases

PaxDbiP41777.
PRIDEiP41777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64896.
KEGGirno:64896.

Organism-specific databases

CTDi9221.
RGDi621578. Nolc1.

Phylogenomic databases

eggNOGiKOG2992. Eukaryota.
ENOG4111U5P. LUCA.
HOGENOMiHOG000113823.
HOVERGENiHBG075370.
InParanoidiP41777.
PhylomeDBiP41777.

Miscellaneous databases

PROiPR:P41777.

Family and domain databases

InterProiView protein in InterPro
IPR006594. LisH.
IPR007718. SRP40_C.
PfamiView protein in Pfam
PF05022. SRP40_C. 1 hit.
PROSITEiView protein in PROSITE
PS50896. LISH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNOLC1_RAT
AccessioniPrimary (citable) accession number: P41777
Secondary accession number(s): F1LPS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 12, 2017
Last modified: April 12, 2017
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.