Reviewed, UniProtKB/Swiss-Prot P41773 (LIPB_PSEFL)

Names and origin

Protein names	Recommended name:     Lipase     EC=3.1.1.3 Alternative name(s):     Triacylglycerol lipase
Organism	Pseudomonas fluorescens
Taxonomic identifier	294 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family. Contains 3 hemolysin-type calcium-binding repeats.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 476	453	Lipase		PRO_0000017743
Regions
	Repeat	372 – 389	18	Hemolysin-type calcium-binding 1
	Repeat	390 – 407	18	Hemolysin-type calcium-binding 2
	Repeat	410 – 427	18	Hemolysin-type calcium-binding 3
Sites
	Active site	207	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41773-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: FC38C080F0A3BC55	FASTA	476	50,239
	10 20 30 40 50 60 MGIFDYKNLG TEGSKTLFAD AMAITLYSYH NLDNGFAVGY QHNGLGLGLP ATLVGALLGS 70 80 90 100 110 120 TDSQGVIPGI PWNPDSEKAA LEAVQKAGWT PISASALGYA GKVDARGTFF GEKAGYTTAQ 130 140 150 160 170 180 VEVLGKYDDA GKLLEIGIGF RGTSGPRETL ISDSIGDLIS DLLAALGPKD YAKNYAGEAF 190 200 210 220 230 240 GGLLKNVADY AGAHGLTGKD VVVSGHSLGG LAVNSMADLS NYKWAGFYKD ANYVAYASPT 250 260 270 280 290 300 QSAGDKVLNI GYENDPVFRA LDGSSFNLSS LGVHDKPHES TTDNIVSFND HYASTLWNVL 310 320 330 340 350 360 PFSIVNLPTW VSHLPTAYGD GMTRILESGF YDQMTRDSTV IVANLSDPAR ANTWVQDLNR 370 380 390 400 410 420 NAEPHKGNTF IIGSDGNDLI QGGNGADFIE GGKGNDTIRD NSGHNTFLFS GHFGNDRVIG 430 440 450 460 470 YQPTDKLVFK DVQGSTDLRD HAKVVGADTV LTFGADSVTL VGVGHGGLWT EGVVIG

References

[1]	"Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52." Tan Y., Miller K.J. Appl. Environ. Microbiol. 58:1402-1407(1992) [PubMed: 1599260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B52.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M86350 Genomic DNA. Translation: AAA25882.1.
PIR	A43942.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.3. 329.
Family and domain databases
InterPro	IPR001343. Hemolysn_Ca-bd. IPR018512. Hemolysn_Ca-bd_NodA. IPR018511. Hemolysn_Ca-bd_sg. IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF00353. HemolysinCabind. 3 hits. PF01764. Lipase_3. 1 hit. [Graphical view]
PRINTS	PR00313. CABNDNGRPT.
PROSITE	PS00330. HEMOLYSIN_CALCIUM. 1 hit. PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPB_PSEFL

Accession

Primary (citable) accession number: P41773

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 22, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)