P41772 (AMT1_CANGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metal-activated transcriptional activator protein AMT1

Gene names

Name:	AMT1
Ordered Locus Names:	CAGL0I04180g

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

284593 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces › Candida glabrata

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Trans-acting regulatory protein that activates transcription of the MT genes (metallothionein) in response to copper or silver ions.
Subcellular location	Nucleus.
Sequence similarities	Contains 1 copper-fist DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Copper DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 265

265

Metal-activated transcriptional activator protein AMT1

PRO_0000194927

Regions

DNA binding

1 – 40

40

Copper-fist

Sites

Metal binding

11

1

Zinc

Metal binding

14

1

Zinc

Metal binding

23

1

Zinc

Metal binding

25

1

Zinc

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

265

Helix Strand Turn

Details...

Beta strand

2 – 4

3

Beta strand

7 – 11

5

Turn

12 – 17

6

Helix

19 – 22

4

Beta strand

30 – 33

4

Sequences

	Sequence		Length	Mass (Da)
	P41772 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 7AC1E182C4D70F64	FASTA	265	29,426
	10 20 30 40 50 60 MVVINGVKYA CDSCIKSHKA AQCEHNDRPL KILKPRGRPP TTCDHCKDMR KTKNVNPSGS 70 80 90 100 110 120 CNCSKLEKIR QEKGITIEED MLMSGNMDMC LCVRGEPCRC HARRKRTQKS NKKDNLSINS 130 140 150 160 170 180 PTNNSPSPAL SVNIGGMVVA NDDILKSLGP IQNVDLTAPL DFPPNGIDNK PMESFYTQTS 190 200 210 220 230 240 KSDAVDSLEF DHLMNMQMRN DNSLSFPMSA NQNEVGYQFN NEGNNSMNST MKNTITQMDQ 250 260 GNSHSMTLHD IDEILNNGIE LGNVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a metal-activated transcription factor gene from Candida glabrata by complementation in Saccharomyces cerevisiae." Zhou P., Thiele D.J. Proc. Natl. Acad. Sci. U.S.A. 88:6112-6116(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65.
[3]	"Regulation of metallothionein genes by the ACE1 and AMT1 transcription factors." Thorvaldsen J.L., Sewell A.K., McCowen C.L., Winge D.R. J. Biol. Chem. 268:12512-12518(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Solution structure of a zinc domain conserved in yeast copper-regulated transcription factors." Turner R.B., Smith D.L., Zawrotny M.E., Summers M.F., Posewitz M.C., Winge D.R. Nat. Struct. Biol. 5:551-555(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1-42.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M69146 Genomic DNA. Translation: AAA35271.1.
CR380955 Genomic DNA. Translation: CAG60367.1.

PIR

A41116.

RefSeq

XP_447430.1. XM_447430.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CO4	NMR	-	A	1-42	[»]

ProteinModelPortal

P41772.

SMR

P41772. Positions 1-42.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2889368.

KEGG

cgr:CAGL0I04180g.

Phylogenomic databases

OrthoDB

EOG4X0R35.

Family and domain databases

Gene3D

3.90.430.10. 1 hit.

InterPro

IPR001083. Cu_fist_DNA-bd.
[Graphical view]

Pfam

PF00649. Copper-fist. 1 hit.
[Graphical view]

PRINTS

PR00617. COPPERFIST.

SMART

SM00412. Cu_FIST. 1 hit.
[Graphical view]

SUPFAM

SSF57879. Cu_fist_DNA_bd. 1 hit.

PROSITE

PS01119. COPPER_FIST_1. 1 hit.
PS50073. COPPER_FIST_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P41772.

Entry information

Entry name

AMT1_CANGA

Accession

Primary (citable) accession number: P41772

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program