P41758 (PGKH_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phosphoglycerate kinase, chloroplastic EC=2.7.2.3
Organism	Chlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier	3055 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: InterPro reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 60	60	Chloroplast Potential
	Chain	61 – 461	401	Phosphoglycerate kinase, chloroplastic		PRO_0000023890
Regions
	Nucleotide binding	417 – 420	4	ATP By similarity
	Region	83 – 85	3	Substrate binding By similarity
	Region	123 – 126	4	Substrate binding By similarity
Sites
	Binding site	100	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	215	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	357	1	ATP; via carbonyl oxygen By similarity
	Binding site	388	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41758 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: BA886FDD9E4DCCB9	FASTA	461	49,008
	10 20 30 40 50 60 MALSMKMRAN ARVSGRRVAA VAPRVVPFSS ASSSVLRSGF ALRCLWTSAA WAALASVVEA 70 80 90 100 110 120 VKKSVGDLHK ADLEGKRVFV RADLNVPLDK ATLAITDDTR IRAAVPTLKY LLDNGAKVLL 130 140 150 160 170 180 TSHLGRPKGG PEDKYRLTPV VARLSELLGK PVTKVDDCIG PEVEKAVGAM KNGELLLLEN 190 200 210 220 230 240 CRFYKEEEKN EPEFAKKLAA NADLYVNDAF GTAHRAHAST EGVTKFLKPS VAGFLLQKEL 250 260 270 280 290 300 DYLDGAVSNP KRPFVAIVGG SKVSSKITVI EALMEKCDKI IIGGGMIFTF YKARALKVGS 310 320 330 340 350 360 SLVEDDKIEL AKKLEEMAKA KGVQLLLPTD VVVADKFDAN ANTQTVPITA IPDGWMGLDI 370 380 390 400 410 420 GPDSVKTFND ALADAKTVVW NGPMGVFEFP QVRQRTVSIA NTLAGLTPKG CITIIGGGDS 430 440 450 460 VAAVEQAGVA EKMSHISTGG GASLELLEGK VLPGVAALDE K

References

[1]	"Purification and cDNA isolation of chloroplastic phosphoglycerate kinase from Chlamydomonas reinhardtii." Kitayama M., Togasaki R.K. Plant Physiol. 107:393-400(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14912 mRNA. Translation: AAA70082.1.
PIR	T08041.
3D structure databases
ProteinModelPortal	P41758.
SMR	P41758. Positions 62-461.
ModBase	Search...
Proteomic databases
PRIDE	P41758.
ProMEX	P41758.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG0126.
Enzyme and pathway databases
UniPathway	UPA00116.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKH_CHLRE

Accession

Primary (citable) accession number: P41758

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program