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P41758

- PGKH_CHLRE

UniProt

P41758 - PGKH_CHLRE

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Protein
Phosphoglycerate kinase, chloroplastic
Gene
N/A
Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001Substrate By similarity
Binding sitei182 – 1821Substrate By similarity
Binding sitei215 – 2151Substrate By similarity
Binding sitei266 – 2661ATP By similarity
Binding sitei357 – 3571ATP; via carbonyl oxygen By similarity
Binding sitei388 – 3881ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi417 – 4204ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoglycerate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: InterPro
  2. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  3. response to cadmium ion Source: EnsemblPlants/Gramene
  4. response to cold Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase, chloroplastic (EC:2.7.2.3)
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: EnsemblPlants/Gramene
  2. cell wall Source: EnsemblPlants/Gramene
  3. chloroplast envelope Source: EnsemblPlants/Gramene
  4. chloroplast stroma Source: EnsemblPlants/Gramene
  5. cytosol Source: EnsemblPlants/Gramene
  6. mitochondrion Source: EnsemblPlants/Gramene
  7. nucleus Source: EnsemblPlants/Gramene
  8. plasma membrane Source: EnsemblPlants/Gramene
  9. plasmodesma Source: EnsemblPlants/Gramene
  10. stromule Source: EnsemblPlants/Gramene
  11. thylakoid Source: EnsemblPlants/Gramene
  12. vacuolar membrane Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6060Chloroplast Reviewed prediction
Add
BLAST
Chaini61 – 461401Phosphoglycerate kinase, chloroplasticUniRule annotation
PRO_0000023890Add
BLAST

Proteomic databases

PRIDEiP41758.
ProMEXiP41758.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP41758.
SMRiP41758. Positions 62-461.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 853Substrate binding By similarity
Regioni123 – 1264Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0126.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41758-1 [UniParc]FASTAAdd to Basket

« Hide

MALSMKMRAN ARVSGRRVAA VAPRVVPFSS ASSSVLRSGF ALRCLWTSAA    50
WAALASVVEA VKKSVGDLHK ADLEGKRVFV RADLNVPLDK ATLAITDDTR 100
IRAAVPTLKY LLDNGAKVLL TSHLGRPKGG PEDKYRLTPV VARLSELLGK 150
PVTKVDDCIG PEVEKAVGAM KNGELLLLEN CRFYKEEEKN EPEFAKKLAA 200
NADLYVNDAF GTAHRAHAST EGVTKFLKPS VAGFLLQKEL DYLDGAVSNP 250
KRPFVAIVGG SKVSSKITVI EALMEKCDKI IIGGGMIFTF YKARALKVGS 300
SLVEDDKIEL AKKLEEMAKA KGVQLLLPTD VVVADKFDAN ANTQTVPITA 350
IPDGWMGLDI GPDSVKTFND ALADAKTVVW NGPMGVFEFP QVRQRTVSIA 400
NTLAGLTPKG CITIIGGGDS VAAVEQAGVA EKMSHISTGG GASLELLEGK 450
VLPGVAALDE K 461
Length:461
Mass (Da):49,008
Last modified:November 1, 1995 - v1
Checksum:iBA886FDD9E4DCCB9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14912 mRNA. Translation: AAA70082.1.
PIRiT08041.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U14912 mRNA. Translation: AAA70082.1 .
PIRi T08041.

3D structure databases

ProteinModelPortali P41758.
SMRi P41758. Positions 62-461.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P41758.
ProMEXi P41758.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0126.

Enzyme and pathway databases

UniPathwayi UPA00116 .

Family and domain databases

Gene3Di 3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPi MF_00145. Phosphoglyc_kinase.
InterProi IPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view ]
PANTHERi PTHR11406. PTHR11406. 1 hit.
Pfami PF00162. PGK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000724. Pgk. 1 hit.
PRINTSi PR00477. PHGLYCKINASE.
SUPFAMi SSF53748. SSF53748. 1 hit.
PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification and cDNA isolation of chloroplastic phosphoglycerate kinase from Chlamydomonas reinhardtii."
    Kitayama M., Togasaki R.K.
    Plant Physiol. 107:393-400(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiPGKH_CHLRE
AccessioniPrimary (citable) accession number: P41758
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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