Reviewed, UniProtKB/Swiss-Prot P41756 (PGK_ASPOR)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase
    EC=2.7.2.3

Gene names

Name:	pgkA
ORF Names:	AO090038000395

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Phosphoglycerate kinase		PRO_0000145875
Regions
	Nucleotide binding	373 – 376	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	64 – 67	4	Substrate binding By similarity
Sites
	Binding site	40	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	171	1	Substrate By similarity
	Binding site	220	1	ATP By similarity
	Binding site	313	1	ATP; via carbonyl oxygen By similarity
	Binding site	344	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41756-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6A25309DF5922A5A	FASTA	417	44,383
	10 20 30 40 50 60 MSLSNKLAIT DVDLKDKRVL IRVDFNVPLD ADKKITNNQR IVGALPTIKY AIENGAKAVV 70 80 90 100 110 120 LMSHLGRPDG KANPKYSLKP VATELEKLLS KSVIFAENCV GKETEEIVNK ATGGQIILLE 130 140 150 160 170 180 NLRFHAEEEG SSKDAEGKKV KADKEKVEEF RKGLTALGDV YINDAFGTAH RAHSSMVGVD 190 200 210 220 230 240 LPQKASGFLV KKELEYFAKA LESPQRPFLA ILGGAKVSDK IQLIDNLLPK VNSLIITGAM 250 260 270 280 290 300 AFTFKKTLEN VKIGNSLFDE AGSKIVGDIV EKAKKNNVKI VLPVDYVTAD KFAADAKTGY 310 320 330 340 350 360 ATDADGIPDG YMGLDVGEKS VELYKKTIAE AKTILWNGPP GVFELEPFAN ATKKTLDAAV 370 380 390 400 410 AAAQSGSIVI IGGGDTATVA AKYGAEAKLS HVSTGGGASL ELLEGKVLPG VDALSSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the phosphoglycerate kinase gene (pgkA) from Aspergillus oryzae." Ogawa M., Kitamoto K., Gomi K., Kumagai C., Tamura G. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D28484 Genomic DNA. Translation: BAA05843.1. AP007169 Genomic DNA. Translation: BAE64188.1.
RefSeq	XP_001825321.1.
3D structure databases
HSSP	HSSP built from PDB template 1QPG based on UniProtKB P00560.
ModBase	Search...
Genome annotation databases
GeneID	5997416.
GenomeReviews	Gene locus pgkA in contig AP007169_GR.
KEGG	aor:AO090038000395.
Enzyme and pathway databases
BRENDA	2.7.2.3. 2240.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_ASPOR

Accession

Primary (citable) accession number: P41756
Secondary accession number(s): Q2U2M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 22, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)