Reviewed, UniProtKB/Swiss-Prot P41755 (DHE2_ACHKL)

Names and origin

Protein names	Recommended name:     NAD-specific glutamate dehydrogenase       Short name=NAD-GDH     EC=1.4.1.2
Organism	Achlya klebsiana
Taxonomic identifier	4767 [NCBI]
Taxonomic lineage	Eukaryota › stramenopiles › Oomycetes › Saprolegniales › Saprolegniaceae › Achlya

Protein attributes

Sequence length	1063 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH.
Enzyme regulation	Allosterically activated by NADP(+).
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family. Highly divergent.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1063	1063	NAD-specific glutamate dehydrogenase		PRO_0000182731

Sequences

	Sequence		Length	Mass (Da)
	P41755-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4EAC1E2C36674860	FASTA	1,063	118,741
	10 20 30 40 50 60 MLLCIVLFLI FHILINQAGI VHVLYNASFV ASSSRFILFC EELFKATPQY WQWGNSIVQN 70 80 90 100 110 120 GDASIASTLY LTMRTFIKHW KYTLLIMINQ SISYSCYSTN EASHLLFTNL PKVAPYTLGP 130 140 150 160 170 180 LNFECCLLSS HHIRHIVLMF YGANIDHGLT KYELYTVCRP SSDEKLCNKI LNSPRLHTHN 190 200 210 220 230 240 RYLYLTEVRI VGAFYLIKQV GARMVGLLVH LAFMVLQNLP KLIWPLDLVH VRQKGTCTAH 250 260 270 280 290 300 IAYHPLAQKH FPPYFTHFYL NVTIYAVVKN ILDNISSSFT LQSNRVRPRP DVAGFDFFHR 310 320 330 340 350 360 FDAFEQSLTY GGDTGITSSN EKVALCFEAT YTDIFFTVEI IDSTSVDSGA IMLPILVDAY 370 380 390 400 410 420 TAVARNFCSV SMVISPLVTL VVYDIYTCSF IGLCTQRRTI NCTRILDAIS SVGNSHTFLP 430 440 450 460 470 480 RYKSPRELTT RQPSGSSVIL LIFLLLGFEF FLFSGLVVVE PVNDVGDLVV NDLLVAFIDL 490 500 510 520 530 540 ALELFVVEGV AEVVGVVFKT VLGFNADVVG FIFRLVLFSF LHHAFNIILG EATLVVGNGN 550 560 570 580 590 600 LVFFTSRLFD GRHVQDTVGI NVEGDINLWN TTRHWRNTIE GELPEQVVVT GHRTLTFKHL 610 620 630 640 650 660 NQHTRLVVSV GGESLRLLGW HSSVTLDEGS HDTTSSFQTK RERSDIKKQQ VLELFRRVVT 670 680 690 700 710 720 AQNGSLDCGT ESNSFIRVDR LAWFLAVEEV RKQLLDLWDT GGTTDKDDFM DLALGELRVT 730 740 750 760 770 780 EDLFNRFHSL AEVVTAHVFE TGTGDGGVEI NTIEERVDFN VSLGRRRKST LGTFTSGTKT 790 800 810 820 830 840 AKGTLVLGHI LAVLALEFSG KVVDEAVIEI FTTQVGITSS SLDFEDTFFN GQKRHIEGTT 850 860 870 880 890 900 TKIENENIAF TTLLVKTVGN GGTSRFVNDT KDVKTSNGTS ILGSLTLRVV EISWDGNDSV 910 920 930 940 950 960 VNSSTNEGFS NFLHLDQNHR GNFFRLESLS FTLEFDGDLW LVTSTRGNLE WPVLNIGLSS 970 980 990 1000 1010 1020 WVVEFTTDQT LSIEHSVGRV HGNLVLSGIT NKTFAVSESN VRWGGTVTLI VGNNFNTIVL 1030 1040 1050 1060 PDTDTRISRT EIDTDGSSLD TRHACSKFKN EVDSPPHLTS AFI

References

[1]

"Molecular characterization of an NAD-specific glutamate dehydrogenase gene inducible by L-glutamine. Antisense gene pair arrangement with L-glutamine-inducible heat shock 70-like protein gene." Lejohn H.B., Cameron L.E., Yang B., Rennie S.L. J. Biol. Chem. 269:4523-4531(1994) [PubMed: 8308022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U02505 Unassigned DNA. Translation: AAA17563.1.
PIR	A53164.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE2_ACHKL

Accession

Primary (citable) accession number: P41755

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 4, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)